Hellen, Peter

[["dc.bibliographiccitation.artnumber","19"],["dc.bibliographiccitation.journal","The Journal of Headache and Pain"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Neeb, Lars"],["dc.contributor.author","Hellen, Peter"],["dc.contributor.author","Hoffmann, Jan"],["dc.contributor.author","Dirnagl, Ulrich"],["dc.contributor.author","Reuter, Uwe"],["dc.date.accessioned","2018-11-07T10:17:50Z"],["dc.date.available","2018-11-07T10:17:50Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: Methylprednisolone (MPD) is a rapid acting highly effective cluster headache preventive and also suppresses the recurrence of migraine attacks. Previously, we could demonstrate that elevated CGRP plasma levels in a cluster headache bout are normalized after a course of high dose corticosteroids. Here we assess whether MPD suppresses interleukin-1 beta (IL-1 beta)- and prostaglandin E-2 (PGE(2))-induced CGRP release in a cell culture model of trigeminal ganglia cells, which could account for the preventive effect in migraine and cluster headache. Metoprololi >>(MTP), a migraine preventive with a slow onset of action, was used for comparison. Methods: Primary cultures of rat trigeminal ganglia were stimulated for 24 h with 10 ng/ml IL-1 beta or for 4 h with 10 mu M PGE(2) following the exposure to 10 or 100 mu M MPD or 100 nM or 10 A mu M MTP for 45 min or 24 h. CGRP was determined by using a commercial enzyme immunoassay. Results: MPD but not MTP blocked IL-1 beta-induced CGRP release from cultured trigeminal cells. PGE(2)-stimulated CGRP release from trigeminal ganglia cell culture was not affected by pre-stimulation whether with MPD or MTP. Conclusion: MPD but not MTP suppresses cytokine (IL-1 beta)-induced CGRP release from trigeminal ganglia cells. We propose that blockade of cytokine mediated trigeminal activation may represent a potential mechanism of action that mediates the preventive effect of MTP on cluster headache and recurrent migraine attacks."],["dc.description.sponsorship","Bundesministerium fur Bildung und Forschung (BMBF) [01EM 0515]"],["dc.identifier.doi","10.1186/s10194-016-0609-x"],["dc.identifier.isi","000371424500001"],["dc.identifier.pmid","26931452"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13180"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41306"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1129-2377"],["dc.relation.issn","1129-2369"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Methylprednisolone blocks interleukin 1 beta induced calcitonin gene related peptide release in trigeminal ganglia cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]