Reuß, Bernhard M.

[["dc.bibliographiccitation.artnumber","3641"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Bernhardt, M."],["dc.contributor.author","Nicolas, J.-D."],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Mittelstädt, H."],["dc.contributor.author","Reuß, Bernhard M."],["dc.contributor.author","Harke, B."],["dc.contributor.author","Wittmeier, A."],["dc.contributor.author","Sprung, M."],["dc.contributor.author","Köster, S."],["dc.contributor.author","Salditt, T."],["dc.date.accessioned","2020-03-10T11:45:32Z"],["dc.date.available","2020-03-10T11:45:32Z"],["dc.date.issued","2018"],["dc.description.abstract","We present a correlative microscopy approach for biology based on holographic X-ray imaging, X-ray scanning diffraction, and stimulated emission depletion (STED) microscopy. All modalities are combined into the same synchrotron endstation. In this way, labeled and unlabeled structures in cells are visualized in a complementary manner. We map out the fluorescently labeled actin cytoskeleton in heart tissue cells and superimpose the data with phase maps from X-ray holography. Furthermore, an array of local far-field diffraction patterns is recorded in the regime of small-angle X-ray scattering (scanning SAXS), which can be interpreted in terms of biomolecular shape and spatial correlations of all contributing scattering constituents. We find that principal directions of anisotropic diffraction patterns coincide to a certain degree with the actin fiber directions and that actin stands out in the phase maps from holographic recordings. In situ STED recordings are proposed to formulate models for diffraction data based on co-localization constraints."],["dc.identifier.doi","10.1038/s41467-018-05885-z"],["dc.identifier.pmid","30194418"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15331"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63281"],["dc.language.iso","en"],["dc.relation.issn","2041-1723"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","530"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.title","Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","579"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Cell Biology and Toxicology"],["dc.bibliographiccitation.lastpage","591"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Dahm, Liane"],["dc.contributor.author","Klugmann, Fanny"],["dc.contributor.author","Gonzalez-Algaba, Angeles"],["dc.contributor.author","Reuss, Bernhard"],["dc.date.accessioned","2018-11-07T08:36:04Z"],["dc.date.available","2018-11-07T08:36:04Z"],["dc.date.issued","2010"],["dc.description.abstract","Gap junctions (GJ) represent a cellular communication system known to influence neuronal differentiation and survival. To assess a putative role of this system for neural effects of tamoxifen (TAM) and raloxifene (RAL), we used the human teratocarcinoma cell line NTera2/D1, retinoic acid (RA)-dependent neuronal differentiation of which is regulated by gap junctions formed of connexin43 (Cx43). As demonstrated by Western blot analysis, concentrations above 1 A mu mol/l for TAM, and 0.1 A mu mol/l for RAL lead to a temporary time- and concentration-dependent increase in Cx43 immunoreactivity, which reached a peak for TAM after 1 day and for RAL after 2 days. Immunocytochemical stainings revealed the increase in Cx43 immunoreactivity to result from an accumulation in intracellular compartments such as the Golgi apparatus or lysosomes. In addition, TAM and RAL were able to prevent the RA-dependent decrease of Cx43 immunoreactivity in NTera2/D1 cells, normally observed during neuronal differentiation. This suggested a suppression of neuronal differentiation to result from these substances. According to this, treatment of NTera2/D1 cells with 10 A mu mol/l TAM or RAL during weeks 1 and 2 of a 6 weeks RA-driven differentiation schedule impaired, whereas treatment during weeks 5 and 6 did not impair, neuronal differentiation of these cells. Modulation of GJ coupling between NTera2/D1 cells by TAM and RAL seems therefore to perturb early neuronal differentiation, whereas differentiated neurons in the mature brain seem to be not affected. These effects could be of importance for actions of TAM and RAL on early embryonic steps of nervous system formation."],["dc.identifier.doi","10.1007/s10565-010-9165-3"],["dc.identifier.isi","000283360100007"],["dc.identifier.pmid","20437090"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7649"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18220"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0742-2091"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Tamoxifen and raloxifene modulate gap junction coupling during early phases of retinoic acid-dependent neuronal differentiation of NTera2/D1 cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]