Brakemann, Tanja

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","European biophysics journal : with biophysics letters"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Brakemann, Tanja"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:45:52Z"],["dc.date.available","2017-09-07T11:45:52Z"],["dc.date.issued","2011"],["dc.identifier.gro","3145539"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3248"],["dc.notes.intern","lifescience"],["dc.notes.status","public"],["dc.notes.submitter","oschaef1"],["dc.publisher","Springer"],["dc.relation.conference","8th EBSA European Biophysics Congress"],["dc.relation.eissn","1432-1017"],["dc.relation.eventend","2011-08-27"],["dc.relation.eventlocation","Budapest, Hungary"],["dc.relation.eventstart","2011-08-23"],["dc.relation.issn","0175-7571"],["dc.title","A new class of reversibly switchable fluorescent proteins"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Protein Science / Supplement"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Brakemann, Tanja"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Weber, Gert"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Wahl, Markus C."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:45:52Z"],["dc.date.available","2017-09-07T11:45:52Z"],["dc.date.issued","2012"],["dc.format.extent","164"],["dc.identifier.gro","3145551"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3261"],["dc.notes.intern","lifescience"],["dc.notes.status","public"],["dc.notes.submitter","oschaef1"],["dc.relation.eissn","1469-896X"],["dc.relation.issn","0961-8368"],["dc.title","Dreiklang - the one, two, three in photoswitching"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","942"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Nature Biotechnology"],["dc.bibliographiccitation.lastpage","U132"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Brakemann, Tanja"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Weber, Gert"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Plessmann, Uwe"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Wahl, Markus C."],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:43:22Z"],["dc.date.available","2017-09-07T11:43:22Z"],["dc.date.issued","2011"],["dc.description.abstract","Photoswitchable fluorescent proteins have enabled new approaches for imaging cells, but their utility has been limited either because they cannot be switched repeatedly or because the wavelengths for switching and fluorescence imaging are strictly coupled. We report a bright, monomeric, reversibly photoswitchable variant of GFP, Dreiklang, whose fluorescence excitation spectrum is decoupled from that for optical switching. Reversible on-and-off switching in living cells is accomplished at illumination wavelengths of similar to 365 nm and similar to 405 nm, respectively, whereas fluorescence is elicited at similar to 515 nm. Mass spectrometry and high-resolution crystallographic analysis of the same protein crystal in the photoswitched on- and off-states demonstrate that switching is based on a reversible hydration/dehydration reaction that modifies the chromophore. The switching properties of Dreiklang enable far-field fluorescence nanoscopy in living mammalian cells using both a coordinate-targeted and a stochastic single molecule switching approach."],["dc.identifier.doi","10.1038/nbt.1952"],["dc.identifier.gro","3142656"],["dc.identifier.isi","000296273000022"],["dc.identifier.pmid","21909082"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Nature Publishing Group"],["dc.relation.eissn","1546-1696"],["dc.relation.issn","1087-0156"],["dc.title","A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14603"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Journal of biological chemistry"],["dc.bibliographiccitation.lastpage","14609"],["dc.bibliographiccitation.volume","285"],["dc.contributor.author","Brakemann, Tanja"],["dc.contributor.author","Weber, Gert"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Groenhof, Gerrit"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Trowitzsch, Simon"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Grubmüller, Helmut"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Wahl, Markus C."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:46:04Z"],["dc.date.available","2017-09-07T11:46:04Z"],["dc.date.issued","2010"],["dc.description.abstract","Reversibly switchable fluorescent proteins can be repeatedly photoswitched between a fluorescent and a nonfluorescent state by irradiation with the light of two different wavelengths. The molecular basis of the switching process remains a controversial topic. Padron0.9 is a reversibly switchable fluorescent protein with \"positive\" switching characteristics, exhibiting excellent spectroscopic properties. Its chromophore is formed by the amino acids Cys-Tyr-Gly. We obtained high resolution x-ray structures of Padron0.9 in both the fluorescent and the nonfluorescent states and used the structural information for molecular dynamics simulations. We found that in Padron0.9 the chromophore undergoes a cis-trans isomerization upon photoswitching. The molecular dynamics simulations clarified the protonation states of the amino acid residues within the chromophore pocket that influence the protonation state of the chromophore. We conclude that a light driven cis-trans isomerization of the chromophore appears to be the fundamental switching mechanism in all photochromic fluorescent proteins known to date. Distinct absorption cross-sections for the switching wavelengths in the fluorescent and the nonfluorescent state are not essential for efficient photochromism in fluorescent proteins, although they may facilitate the switching process."],["dc.identifier.doi","10.1074/jbc.M109.086314"],["dc.identifier.gro","3142927"],["dc.identifier.isi","000277299700057"],["dc.identifier.pmid","20236929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/385"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0021-9258"],["dc.title","Molecular Basis of the Light-driven Switching of the Photochromic Fluorescent Protein Padron"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]