Bucher, Gregor

[["dc.bibliographiccitation.firstpage","1819"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.lastpage","1824"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Troelenberg, Nicole"],["dc.contributor.author","Dao, Van Anh"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Klingler, Martin"],["dc.date.accessioned","2020-12-10T18:12:48Z"],["dc.date.available","2020-12-10T18:12:48Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1073/pnas.1716512115"],["dc.identifier.eissn","1091-6490"],["dc.identifier.issn","0027-8424"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74503"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Double abdomen in a short-germ insect: Zygotic control of axis formation revealed in the beetle Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","38"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Biology"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Hakeemi, Muhammad S."],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Teuscher, Matthias"],["dc.contributor.author","Weißkopf, Matthias"],["dc.contributor.author","Großmann, Daniela"],["dc.contributor.author","Kessel, Tobias"],["dc.contributor.author","Dönitz, Jürgen"],["dc.contributor.author","Siemanowski, Janna"],["dc.contributor.author","Wan, Xuebin"],["dc.contributor.author","Schultheis, Dorothea"],["dc.contributor.author","Frasch, Manfred"],["dc.contributor.author","Roth, Siegfried"],["dc.contributor.author","Schoppmeier, Michael"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2022-04-01T10:03:06Z"],["dc.date.accessioned","2022-08-18T12:34:50Z"],["dc.date.available","2022-04-01T10:03:06Z"],["dc.date.available","2022-08-18T12:34:50Z"],["dc.date.issued","2022-02-08"],["dc.date.updated","2022-07-29T12:07:11Z"],["dc.description.abstract","Abstract\r\n \r\n Background\r\n Most of the known genes required for developmental processes have been identified by genetic screens in a few well-studied model organisms, which have been considered representative of related species, and informative—to some degree—for human biology. The fruit fly Drosophila melanogaster is a prime model for insect genetics, and while conservation of many gene functions has been observed among bilaterian animals, a plethora of data show evolutionary divergence of gene function among more closely-related groups, such as within the insects. A quantification of conservation versus divergence of gene functions has been missing, without which it is unclear how representative data from model systems actually are.\r\n \r\n \r\n Results\r\n Here, we systematically compare the gene sets required for a number of homologous but divergent developmental processes between fly and beetle in order to quantify the difference of the gene sets. To that end, we expanded our RNAi screen in the red flour beetle Tribolium castaneum to cover more than half of the protein-coding genes. Then we compared the gene sets required for four different developmental processes between beetle and fly. We found that around 50% of the gene functions were identified in the screens of both species while for the rest, phenotypes were revealed only in fly (~ 10%) or beetle (~ 40%) reflecting both technical and biological differences. Accordingly, we were able to annotate novel developmental GO terms for 96 genes studied in this work. With this work, we publish the final dataset for the pupal injection screen of the iBeetle screen reaching a coverage of 87% (13,020 genes).\r\n \r\n \r\n Conclusions\r\n We conclude that the gene sets required for a homologous process diverge more than widely believed. Hence, the insights gained in flies may be less representative for insects or protostomes than previously thought, and work in complementary model systems is required to gain a comprehensive picture. The RNAi screening resources developed in this project, the expanding transgenic toolkit, and our large-scale functional data make T. castaneum an excellent model system in that endeavor."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.citation","BMC Biology. 2022 Feb 08;20(1):38"],["dc.identifier.doi","10.1186/s12915-022-01231-4"],["dc.identifier.pii","1231"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106084"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112934"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.publisher","BioMed Central"],["dc.relation.eissn","1741-7007"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject","Gene function"],["dc.subject","Comparative genomics"],["dc.subject","RNAi screen"],["dc.subject","iBeetle"],["dc.subject","Tribolium castaneum"],["dc.subject","Drosophila melanogaster"],["dc.subject","Divergence of gene function"],["dc.subject","iBeetle-Base"],["dc.subject","FlyBase"],["dc.title","Screens in fly and beetle reveal vastly divergent gene sets required for developmental processes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","608"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Lehmann, Sabrina"],["dc.contributor.author","Atika, Bibi"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Strohlein, Nadi"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Weißkopf, Matthias"],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Teuscher, Matthias"],["dc.contributor.author","Hakeemi, Muhammad S."],["dc.contributor.author","Li, Jianwei"],["dc.contributor.author","Weißbecker, Bernhard"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2022-08-22T06:20:44Z"],["dc.date.available","2022-08-22T06:20:44Z"],["dc.date.issued","2022-08-20"],["dc.date.updated","2022-08-21T03:10:50Z"],["dc.description.abstract","Abstract\n \n Background\n Functional genomics uses unbiased systematic genome-wide gene disruption or analyzes natural variations such as gene expression profiles of different tissues from multicellular organisms to link gene functions to particular phenotypes. Functional genomics approaches are of particular importance to identify large sets of genes that are specifically important for a particular biological process beyond known candidate genes, or when the process has not been studied with genetic methods before.\n \n \n Results\n Here, we present a large set of genes whose disruption interferes with the function of the odoriferous defensive stink glands of the red flour beetle Tribolium castaneum. This gene set is the result of a large-scale systematic phenotypic screen using RNA interference applied in a genome-wide forward genetics manner. In this first-pass screen, 130 genes were identified, of which 69 genes could be confirmed to cause phenotypic changes in the glands upon knock-down, which vary from necrotic tissue and irregular reservoir size to irregular color or separation of the secreted gland compounds. Gene ontology analysis revealed that many of those genes are encoding enzymes (peptidases and cytochromes P450) as well as proteins involved in membrane trafficking with an enrichment in lysosome and mineral absorption pathways. The knock-down of 13 genes caused specifically a strong reduction of para-benzoquinones in the gland reservoirs, suggesting a specific function in the synthesis of these toxic compounds. Only 14 of the 69 confirmed gland genes are differentially overexpressed in stink gland tissue and thus could have been detected in a transcriptome-based analysis. However, only one out of eight genes identified by a transcriptomics approach known to cause phenotypic changes of the glands upon knock-down was recognized by this phenotypic screen, indicating the limitation of such a non-redundant first-pass screen.\n \n \n Conclusion\n Our results indicate the importance of combining diverse and independent methodologies to identify genes necessary for the function of a certain biological tissue, as the different approaches do not deliver redundant results but rather complement each other. The presented phenotypic screen together with a transcriptomics approach are now providing a set of close to hundred genes important for odoriferous defensive stink gland physiology in beetles."],["dc.identifier.citation","BMC Genomics. 2022 Aug 20;23(1):608"],["dc.identifier.doi","10.1186/s12864-022-08822-z"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113084"],["dc.language.iso","en"],["dc.publisher","BioMed Central"],["dc.rights.holder","The Author(s)"],["dc.subject","Chemical ecology"],["dc.subject","Genome-wide"],["dc.subject","iBeetle"],["dc.subject","Odoriferous glands"],["dc.subject","RNA interference"],["dc.subject","RNAseq Tribolium castaneum"],["dc.title","Phenotypic screen and transcriptomics approach complement each other in functional genomics of defensive stink gland physiology"],["dc.type","journal_article"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","g3.200995.2018"],["dc.bibliographiccitation.journal","G3: Genes, Genomes, Genetics"],["dc.contributor.author","Schultheis, Dorothea"],["dc.contributor.author","Weißkopf, Matthias"],["dc.contributor.author","Schaub, Christoph"],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Dao, Van Anh"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Hakeemi, Muhammad Salim"],["dc.contributor.author","Troelenberg, Nicole"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Schwirz, Jonas"],["dc.contributor.author","Ströhlein, Nadia"],["dc.contributor.author","Teuscher, Matthias"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Frasch, Manfred"],["dc.date.accessioned","2020-12-10T18:42:40Z"],["dc.date.available","2020-12-10T18:42:40Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1534/g3.118.200995"],["dc.identifier.eissn","2160-1836"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78042"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","A Large Scale Systemic RNAi Screen in the Red Flour Beetle Tribolium castaneum Identifies Novel Genes Involved in Insect Muscle Development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]