Bucher, Gregor

[["dc.bibliographiccitation.artnumber","73"],["dc.bibliographiccitation.journal","BMC Biology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Trauner, Jochen"],["dc.contributor.author","Schinko, Johannes"],["dc.contributor.author","Lorenzen, Marce D."],["dc.contributor.author","Shippy, Teresa D."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Beeman, Richard W."],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Brown, Susan J."],["dc.date.accessioned","2018-11-07T11:22:11Z"],["dc.date.available","2018-11-07T11:22:11Z"],["dc.date.issued","2009"],["dc.description.abstract","Background: Given its sequenced genome and efficient systemic RNA interference response, the red flour beetle Tribolium castaneum is a model organism well suited for reverse genetics. Even so, there is a pressing need for forward genetic analysis to escape the bias inherent in candidate gene approaches. Results: To produce easy-to-maintain insertional mutations and to obtain fluorescent marker lines to aid phenotypic analysis, we undertook a large-scale transposon mutagenesis screen. In this screen, we produced more than 6,500 new piggyBac insertions. Of these, 421 proved to be recessive lethal, 75 were semi-lethal, and eight indicated recessive sterility, while 505 showed new enhancer-trap patterns. Insertion junctions were determined for 403 lines and often appeared to be located within transcription units. Insertion sites appeared to be randomly distributed throughout the genome, with the exception of a preference for reinsertion near the donor site. Conclusion: A large collection of enhancer-trap and embryonic lethal beetle lines has been made available to the research community and will foster investigations into diverse fields of insect biology, pest control, and evolution. Because the genetic elements used in this screen are species-nonspecific, and because the crossing scheme does not depend on balancer chromosomes, the methods presented herein should be broadly applicable for many insect species."],["dc.identifier.doi","10.1186/1741-7007-7-73"],["dc.identifier.fs","568930"],["dc.identifier.isi","000272336000001"],["dc.identifier.pmid","19891766"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5844"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55941"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1741-7007"],["dc.rights","CC BY 2.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.subject.ddc","570"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Chromosome Mapping"],["dc.subject.mesh","Crosses, Genetic"],["dc.subject.mesh","DNA Transposable Elements"],["dc.subject.mesh","Embryo Loss"],["dc.subject.mesh","Embryo, Nonmammalian"],["dc.subject.mesh","Enhancer Elements, Genetic"],["dc.subject.mesh","Genes, Lethal"],["dc.subject.mesh","Genetic Markers"],["dc.subject.mesh","Germ-Line Mutation"],["dc.subject.mesh","Mutagenesis, Insertional"],["dc.subject.mesh","Transposases"],["dc.subject.mesh","Tribolium"],["dc.title","Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7782"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","7786"],["dc.bibliographiccitation.volume","109"],["dc.contributor.author","Fu, Jinping"],["dc.contributor.author","Posnien, Nico"],["dc.contributor.author","Bolognesi, Renata"],["dc.contributor.author","Fischer, Tamara D."],["dc.contributor.author","Rayl, Parker"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Kitzmann, Peter"],["dc.contributor.author","Brown, Susan J."],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:10:25Z"],["dc.date.available","2018-11-07T09:10:25Z"],["dc.date.issued","2012"],["dc.description.abstract","Canonical Wnt signaling has been implicated in an AP axis polarizing mechanism in most animals, despite limited evidence from arthropods. In the long-germ insect, Drosophila, Wnt signaling is not required for global AP patterning, but in short-germ insects including Tribolium castaneum, loss of Wnt signaling affects development of segments in the growth zone but not those defined in the blastoderm. To determine the effects of ectopic Wnt signaling, we analyzed the expression and function of axin, which encodes a highly conserved negative regulator of the pathway. We found Tc-axin transcripts maternally localized to the anterior pole in freshly laid eggs. Expression spread toward the posterior pole during the early cleavage stages, becoming ubiquitous by the time the germ rudiment formed. Tc-axin RNAi produced progeny phenotypes that ranged from mildly affected embryos with cuticles displaying a graded loss of anterior structures, to defective embryos that condensed at the posterior pole in the absence of serosa. Altered expression domains of several blastodermal markers indicated anterior expansion of posterior fates. Analysis of other canonical Wnt pathway components and the expansion of Tc-caudal expression, a Wnt target, suggest that the effects of Tc-axin depletion are mediated through this pathway and that Wnt signaling must be inhibited for proper anterior development in Tribolium. These studies provide unique evidence that canonical Wnt signaling must be carefully regulated along the AP axis in an arthropod, and support an ancestral role for Wnt activity in defining AP polarity and patterning in metazoan development."],["dc.identifier.doi","10.1073/pnas.1116641109"],["dc.identifier.isi","000304369800049"],["dc.identifier.pmid","22552230"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26486"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","Asymmetrically expressed axin required for anterior development in Tribolium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]