Bucher, Gregor

[["dc.bibliographiccitation.firstpage","284"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","294"],["dc.bibliographiccitation.volume","321"],["dc.contributor.author","Cerny, Alexander C."],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Klingler, Martin"],["dc.date.accessioned","2018-11-07T11:11:15Z"],["dc.date.available","2018-11-07T11:11:15Z"],["dc.date.issued","2008"],["dc.description.abstract","Segment formation in the long germ insect Drosophila is dominated by overlapping gap gene domains in the syncytial blastoderm. In the short germ beetle Tribolium castaneum abdominal segments arise from a cellular growth zone. implying different patterning mechanisms. We describe here the single Tribolium ortholog of the Drosophila genes knirps and knirps-related (called Tc-knirps). Tc-knirps expression is conserved during head patterning and at later stages. However, Posterior Tc-knirps expression in the ectoderm is limited to a stripe in Al. instead of a broad abdominal domain covering segment primordia A2-A5 as in Drosophila. Tc-knirps RNAi yields only mild defects in the abdomen, at a position posterior to the abdominal Tc-knirps domain. In addition, Tc-knirps RNAi larvae lack the antennal and mandibular segments. These defects are much more severe than the head defects Caused by combined inactivation of Din-knirps and Dm-knirps-related. Our findings support the notion that the role of gap gene homologs in abdominal segmentation differs fundamentally in long and short germ insects, Moreover, the pivotal role of Tc-knirps in the head Suggests an ancestral role for knirps as head patterning gene. Based on this RNAi analysis, Tc-knirps functions neither in the head nor the abdomen as a canonical gap gene. (C) 2008 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.ydbio.2008.05.527"],["dc.identifier.isi","000258710800025"],["dc.identifier.pmid","18586236"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53389"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","0012-1606"],["dc.title","The Tribolium ortholog of knirps and knirps-related is crucial for head segmentation but plays a minor role during abdominal patterning"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4740"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Development"],["dc.bibliographiccitation.lastpage","4750"],["dc.bibliographiccitation.volume","141"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Siemanowski, Janna L."],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:31:58Z"],["dc.date.available","2018-11-07T09:31:58Z"],["dc.date.issued","2014"],["dc.description.abstract","Wnt/beta-catenin and hedgehog (Hh) signaling are essential for transmitting signals across cell membranes in animal embryos. Early patterning of the principal insect model, Drosophila melanogaster, occurs in the syncytial blastoderm, where diffusion of transcription factors obviates the need for signaling pathways. However, in the cellularized growth zone of typical short germ insect embryos, signaling pathways are predicted to play a more fundamental role. Indeed, the Wnt/beta-catenin pathway is required for posterior elongation in most arthropods, although which target genes are activated in this context remains elusive. Here, we use the short germ beetle Tribolium castaneum to investigate two Wnt and Hh signaling centers located in the head anlagen and in the growth zone of early embryos. We find that Wnt/beta-catenin signaling acts upstream of Hh in the growth zone, whereas the opposite interaction occurs in the head. We determine the target gene sets of the Wnt/beta-catenin and Hh pathways and find that the growth zone signaling center activates a much greater number of genes and that the Wnt and Hh target gene sets are essentially non-overlapping. The Wnt pathway activates key genes of all three germ layers, including pair-rule genes, and Tc-caudal and Tc-twist. Furthermore, the Wnt pathway is required for hindgut development and we identify Tc-senseless as a novel hindgut patterning gene required in the early growth zone. At the same time, Wnt acts on growth zone metabolism and cell division, thereby integrating growth with patterning. Posterior Hh signaling activates several genes potentially involved in a proteinase cascade of unknown function."],["dc.identifier.doi","10.1242/dev.112797"],["dc.identifier.isi","346431900010"],["dc.identifier.pmid","25395458"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31644"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Company Of Biologists Ltd"],["dc.relation.issn","1477-9129"],["dc.relation.issn","0950-1991"],["dc.title","Wnt/beta-catenin signaling integrates patterning and metabolism of the insect growth zone"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","g3.200995.2018"],["dc.bibliographiccitation.journal","G3: Genes, Genomes, Genetics"],["dc.contributor.author","Schultheis, Dorothea"],["dc.contributor.author","Weißkopf, Matthias"],["dc.contributor.author","Schaub, Christoph"],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Dao, Van Anh"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Hakeemi, Muhammad Salim"],["dc.contributor.author","Troelenberg, Nicole"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Schwirz, Jonas"],["dc.contributor.author","Ströhlein, Nadia"],["dc.contributor.author","Teuscher, Matthias"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Frasch, Manfred"],["dc.date.accessioned","2020-12-10T18:42:40Z"],["dc.date.available","2020-12-10T18:42:40Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1534/g3.118.200995"],["dc.identifier.eissn","2160-1836"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78042"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","A Large Scale Systemic RNAi Screen in the Red Flour Beetle Tribolium castaneum Identifies Novel Genes Involved in Insect Muscle Development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]