Bucher, Gregor

[["dc.bibliographiccitation.artnumber","25"],["dc.bibliographiccitation.journal","BMC Developmental Biology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Peel, Andrew D."],["dc.contributor.author","Schanda, Julia"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Ruge, Frank"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Gilles, Anna F."],["dc.contributor.author","Schinko, Johannes B."],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:23:38Z"],["dc.date.available","2018-11-07T09:23:38Z"],["dc.date.issued","2013"],["dc.description.abstract","Background: The Drosophila larval head is evolutionarily derived at the genetic and morphological level. In the beetle Tribolium castaneum, development of the larval head more closely resembles the ancestral arthropod condition. Unlike in Drosophila, a knirps homologue (Tc-kni) is required for development of the antennae and mandibles. However, published Tc-kni data are restricted to cuticle phenotypes and Tc-even-skipped and Tc-wingless stainings in knockdown embryos. Hence, it has remained unclear whether the entire antennal and mandibular segments depend on Tc-kni function, and whether the intervening intercalary segment is formed completely. We address these questions with a detailed examination of Tc-kni function. Results: By examining the expression of marker genes in RNAi embryos, we show that Tc-kni is required only for the formation of the posterior parts of the antennal and mandibular segments (i.e. the parasegmental boundaries). Moreover, we find that the role of Tc-kni is distinct in these segments: Tc-kni is required for the initiation of the antennal parasegment boundary, but only for the maintenance of the mandibular parasegmental boundary. Surprisingly, Tc-kni controls the timing of expression of the Hox gene Tc-labial in the intercalary segment, although this segment does form in the absence of Tc-kni function. Unexpectedly, we find that the pair-rule gene Tc-even-skipped helps set the posterior boundary of Tc-kni expression in the mandible. Using the mutant antennaless, a likely regulatory Null mutation at the Tc-kni locus, we provide evidence that our RNAi studies represent a Null situation. Conclusions: Tc-kni is required for the initiation of the antennal and the maintenance of the mandibular parasegmental boundaries. Tc-kni is not required for specification of the anterior regions of these segments, nor the intervening intercalary segment, confirming that Tc-kni is not a canonical 'gap-gene'. Our finding that a gap gene orthologue is regulated by a pair rule gene adds to the view that the segmentation gene hierarchies differ between Tribolium and Drosophila upstream of the pair rule gene level. In Tribolium, as in Drosophila, head and trunk segmentation gene networks cooperate to pattern the mandibular segment, albeit involving Tc-kni as novel component."],["dc.description.sponsorship","Deutsche Forschunggemeinschaft DFG [BU-1443/3-1]; DFG [Kl656/2]"],["dc.identifier.doi","10.1186/1471-213X-13-25"],["dc.identifier.isi","000321137000001"],["dc.identifier.pmid","23777260"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9126"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29627"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-213X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Tc-knirps plays different roles in the specification of antennal and mandibular parasegment boundaries and is regulated by a pair-rule gene in the beetle Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","D720"],["dc.bibliographiccitation.issue","D1"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.lastpage","D725"],["dc.bibliographiccitation.volume","43"],["dc.contributor.author","Doenitz, Juergen"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Gerischer, Lizzy"],["dc.contributor.author","Tech, Maike"],["dc.contributor.author","Schoppmeier, Michael"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T10:01:53Z"],["dc.date.available","2018-11-07T10:01:53Z"],["dc.date.issued","2015"],["dc.description.abstract","The iBeetle-Base (http://ibeetle-base.uni-goettingen.de) makes available annotations of RNAi phenotypes, which were gathered in a large scale RNAi screen in the red flour beetle Tribolium castaneum (iBeetle screen). In addition, it provides access to sequence information and links for all Tribolium cas-taneum genes. The iBeetle-Base contains the annotations of phenotypes of several thousands of genes knocked down during embryonic and metamorphic epidermis and muscle development in addition to phenotypes linked to oogenesis and stink gland biology. The phenotypes are described according to the EQM (entity, quality, modifier) system using controlled vocabularies and the Tribolium morphological ontology (TrOn). Furthermore, images linked to the respective annotations are provided. The data are searchable either for specific phenotypes using a complex 'search for morphological defects' or a 'quick search' for gene names and IDs. The red flour beetle Tribolium castaneum has become an important model system for insect functional genetics and is a representative of the most species rich taxon, the Coleoptera, which comprise several devastating pests. It is used for studying insect typical development, the evolution of development and for research on metabolism and pest control. Besides Drosophila, Tribolium is the first insect model organism where large scale unbiased screens have been performed."],["dc.description.sponsorship","Open-Access-Publikationsfunds 2014"],["dc.identifier.doi","10.1093/nar/gku1054"],["dc.identifier.isi","000350210400105"],["dc.identifier.pmid","25378303"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11085"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38124"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1362-4962"],["dc.relation.issn","0305-1048"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","38"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Biology"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Hakeemi, Muhammad S."],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Teuscher, Matthias"],["dc.contributor.author","Weißkopf, Matthias"],["dc.contributor.author","Großmann, Daniela"],["dc.contributor.author","Kessel, Tobias"],["dc.contributor.author","Dönitz, Jürgen"],["dc.contributor.author","Siemanowski, Janna"],["dc.contributor.author","Wan, Xuebin"],["dc.contributor.author","Schultheis, Dorothea"],["dc.contributor.author","Frasch, Manfred"],["dc.contributor.author","Roth, Siegfried"],["dc.contributor.author","Schoppmeier, Michael"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2022-04-01T10:03:06Z"],["dc.date.accessioned","2022-08-18T12:34:50Z"],["dc.date.available","2022-04-01T10:03:06Z"],["dc.date.available","2022-08-18T12:34:50Z"],["dc.date.issued","2022-02-08"],["dc.date.updated","2022-07-29T12:07:11Z"],["dc.description.abstract","Abstract\r\n \r\n Background\r\n Most of the known genes required for developmental processes have been identified by genetic screens in a few well-studied model organisms, which have been considered representative of related species, and informative—to some degree—for human biology. The fruit fly Drosophila melanogaster is a prime model for insect genetics, and while conservation of many gene functions has been observed among bilaterian animals, a plethora of data show evolutionary divergence of gene function among more closely-related groups, such as within the insects. A quantification of conservation versus divergence of gene functions has been missing, without which it is unclear how representative data from model systems actually are.\r\n \r\n \r\n Results\r\n Here, we systematically compare the gene sets required for a number of homologous but divergent developmental processes between fly and beetle in order to quantify the difference of the gene sets. To that end, we expanded our RNAi screen in the red flour beetle Tribolium castaneum to cover more than half of the protein-coding genes. Then we compared the gene sets required for four different developmental processes between beetle and fly. We found that around 50% of the gene functions were identified in the screens of both species while for the rest, phenotypes were revealed only in fly (~ 10%) or beetle (~ 40%) reflecting both technical and biological differences. Accordingly, we were able to annotate novel developmental GO terms for 96 genes studied in this work. With this work, we publish the final dataset for the pupal injection screen of the iBeetle screen reaching a coverage of 87% (13,020 genes).\r\n \r\n \r\n Conclusions\r\n We conclude that the gene sets required for a homologous process diverge more than widely believed. Hence, the insights gained in flies may be less representative for insects or protostomes than previously thought, and work in complementary model systems is required to gain a comprehensive picture. The RNAi screening resources developed in this project, the expanding transgenic toolkit, and our large-scale functional data make T. castaneum an excellent model system in that endeavor."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.citation","BMC Biology. 2022 Feb 08;20(1):38"],["dc.identifier.doi","10.1186/s12915-022-01231-4"],["dc.identifier.pii","1231"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106084"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112934"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.publisher","BioMed Central"],["dc.relation.eissn","1741-7007"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject","Gene function"],["dc.subject","Comparative genomics"],["dc.subject","RNAi screen"],["dc.subject","iBeetle"],["dc.subject","Tribolium castaneum"],["dc.subject","Drosophila melanogaster"],["dc.subject","Divergence of gene function"],["dc.subject","iBeetle-Base"],["dc.subject","FlyBase"],["dc.title","Screens in fly and beetle reveal vastly divergent gene sets required for developmental processes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","608"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Lehmann, Sabrina"],["dc.contributor.author","Atika, Bibi"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Strohlein, Nadi"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Weißkopf, Matthias"],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Teuscher, Matthias"],["dc.contributor.author","Hakeemi, Muhammad S."],["dc.contributor.author","Li, Jianwei"],["dc.contributor.author","Weißbecker, Bernhard"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2022-08-22T06:20:44Z"],["dc.date.available","2022-08-22T06:20:44Z"],["dc.date.issued","2022-08-20"],["dc.date.updated","2022-08-21T03:10:50Z"],["dc.description.abstract","Abstract\n \n Background\n Functional genomics uses unbiased systematic genome-wide gene disruption or analyzes natural variations such as gene expression profiles of different tissues from multicellular organisms to link gene functions to particular phenotypes. Functional genomics approaches are of particular importance to identify large sets of genes that are specifically important for a particular biological process beyond known candidate genes, or when the process has not been studied with genetic methods before.\n \n \n Results\n Here, we present a large set of genes whose disruption interferes with the function of the odoriferous defensive stink glands of the red flour beetle Tribolium castaneum. This gene set is the result of a large-scale systematic phenotypic screen using RNA interference applied in a genome-wide forward genetics manner. In this first-pass screen, 130 genes were identified, of which 69 genes could be confirmed to cause phenotypic changes in the glands upon knock-down, which vary from necrotic tissue and irregular reservoir size to irregular color or separation of the secreted gland compounds. Gene ontology analysis revealed that many of those genes are encoding enzymes (peptidases and cytochromes P450) as well as proteins involved in membrane trafficking with an enrichment in lysosome and mineral absorption pathways. The knock-down of 13 genes caused specifically a strong reduction of para-benzoquinones in the gland reservoirs, suggesting a specific function in the synthesis of these toxic compounds. Only 14 of the 69 confirmed gland genes are differentially overexpressed in stink gland tissue and thus could have been detected in a transcriptome-based analysis. However, only one out of eight genes identified by a transcriptomics approach known to cause phenotypic changes of the glands upon knock-down was recognized by this phenotypic screen, indicating the limitation of such a non-redundant first-pass screen.\n \n \n Conclusion\n Our results indicate the importance of combining diverse and independent methodologies to identify genes necessary for the function of a certain biological tissue, as the different approaches do not deliver redundant results but rather complement each other. The presented phenotypic screen together with a transcriptomics approach are now providing a set of close to hundred genes important for odoriferous defensive stink gland physiology in beetles."],["dc.identifier.citation","BMC Genomics. 2022 Aug 20;23(1):608"],["dc.identifier.doi","10.1186/s12864-022-08822-z"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113084"],["dc.language.iso","en"],["dc.publisher","BioMed Central"],["dc.rights.holder","The Author(s)"],["dc.subject","Chemical ecology"],["dc.subject","Genome-wide"],["dc.subject","iBeetle"],["dc.subject","Odoriferous glands"],["dc.subject","RNA interference"],["dc.subject","RNAseq Tribolium castaneum"],["dc.title","Phenotypic screen and transcriptomics approach complement each other in functional genomics of defensive stink gland physiology"],["dc.type","journal_article"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","14"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","EvoDevo"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2022-08-04T11:57:52Z"],["dc.date.available","2022-08-04T11:57:52Z"],["dc.date.issued","2022-07-19"],["dc.date.updated","2022-07-25T11:18:58Z"],["dc.description.abstract","The red flour beetle Tribolium castaneum has emerged as an important insect model system for a variety of topics. With respect to studying gene function, it is second only to the vinegar fly D. melanogaster. The RNAi response in T. castaneum is exceptionally strong and systemic, and it appears to target all cell types and processes. Uniquely for emerging model organisms, T. castaneum offers the opportunity of performing time- and cost-efficient large-scale RNAi screening, based on commercially available dsRNAs targeting all genes, which are simply injected into the body cavity. Well established transgenic and genome editing approaches are met by ease of husbandry and a relatively short generation time. Consequently, a number of transgenic tools like UAS/Gal4, Cre/Lox, imaging lines and enhancer trap lines are already available. T. castaneum has been a genetic experimental system for decades and now has become a workhorse for molecular and reverse genetics as well as in vivo imaging. Many aspects of development and general biology are more insect-typical in this beetle compared to D. melanogaster. Thus, studying beetle orthologs of well-described fly genes has allowed macro-evolutionary comparisons in developmental processes such as axis formation, body segmentation, and appendage, head and brain development. Transgenic approaches have opened new ways for in vivo imaging. Moreover, this emerging model system is the first choice for research on processes that are not represented in the fly, or are difficult to study there, e.g. extraembryonic tissues, cryptonephridial organs, stink gland function, or dsRNA-based pesticides."],["dc.identifier.citation","EvoDevo. 2022 Jul 19;13(1):14"],["dc.identifier.doi","10.1186/s13227-022-00201-9"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112640"],["dc.language.iso","en"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.subject","Insect model system"],["dc.subject","Arthropod model system"],["dc.subject","Coleoptera"],["dc.subject","Evolution of development"],["dc.subject","Physiology"],["dc.subject","Behavior"],["dc.subject","Pest control"],["dc.subject","Systemic RNAi"],["dc.subject","Genome-wide screen"],["dc.subject","CRISPR genome editing"],["dc.title","The red flour beetle T. castaneum: elaborate genetic toolkit and unbiased large scale RNAi screening to study insect biology and evolution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","7822"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Schultheis, Dorothea"],["dc.contributor.author","Schwirz, Jonas"],["dc.contributor.author","Stroehlein, Nadi"],["dc.contributor.author","Troelenberg, Nicole"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Dao, Van Anh"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Tech, Maike"],["dc.contributor.author","Doenitz, Juergen"],["dc.contributor.author","Gerischer, Lizzy"],["dc.contributor.author","Theis, Mirko"],["dc.contributor.author","Schild, Inga"],["dc.contributor.author","Trauner, Jochen"],["dc.contributor.author","Koniszewski, Nikolaus Dieter Bernhard"],["dc.contributor.author","Kuester, Elke"],["dc.contributor.author","Kittelmann, Sebastian"],["dc.contributor.author","Hu, Yonggang"],["dc.contributor.author","Lehmann, Sabrina"],["dc.contributor.author","Siemanowski, Janna L."],["dc.contributor.author","Ulrich, Julia"],["dc.contributor.author","Panfilio, Kristen A."],["dc.contributor.author","Schroeder, Reinhard"],["dc.contributor.author","Morgenstern, Burkhard"],["dc.contributor.author","Stanke, Mario"],["dc.contributor.author","Buchhholz, Frank"],["dc.contributor.author","Frasch, Manfred"],["dc.contributor.author","Roth, Siegfried"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Schoppmeier, Michael"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:55:00Z"],["dc.date.available","2018-11-07T09:55:00Z"],["dc.date.issued","2015"],["dc.description.abstract","Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila."],["dc.description.sponsorship","Open-Access Publikationsfonds 2015"],["dc.identifier.doi","10.1038/ncomms8822"],["dc.identifier.isi","000358860900002"],["dc.identifier.pmid","26215380"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12460"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36659"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","73"],["dc.bibliographiccitation.journal","BMC Biology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Trauner, Jochen"],["dc.contributor.author","Schinko, Johannes"],["dc.contributor.author","Lorenzen, Marce D."],["dc.contributor.author","Shippy, Teresa D."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Beeman, Richard W."],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Brown, Susan J."],["dc.date.accessioned","2018-11-07T11:22:11Z"],["dc.date.available","2018-11-07T11:22:11Z"],["dc.date.issued","2009"],["dc.description.abstract","Background: Given its sequenced genome and efficient systemic RNA interference response, the red flour beetle Tribolium castaneum is a model organism well suited for reverse genetics. Even so, there is a pressing need for forward genetic analysis to escape the bias inherent in candidate gene approaches. Results: To produce easy-to-maintain insertional mutations and to obtain fluorescent marker lines to aid phenotypic analysis, we undertook a large-scale transposon mutagenesis screen. In this screen, we produced more than 6,500 new piggyBac insertions. Of these, 421 proved to be recessive lethal, 75 were semi-lethal, and eight indicated recessive sterility, while 505 showed new enhancer-trap patterns. Insertion junctions were determined for 403 lines and often appeared to be located within transcription units. Insertion sites appeared to be randomly distributed throughout the genome, with the exception of a preference for reinsertion near the donor site. Conclusion: A large collection of enhancer-trap and embryonic lethal beetle lines has been made available to the research community and will foster investigations into diverse fields of insect biology, pest control, and evolution. Because the genetic elements used in this screen are species-nonspecific, and because the crossing scheme does not depend on balancer chromosomes, the methods presented herein should be broadly applicable for many insect species."],["dc.identifier.doi","10.1186/1741-7007-7-73"],["dc.identifier.fs","568930"],["dc.identifier.isi","000272336000001"],["dc.identifier.pmid","19891766"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5844"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55941"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1741-7007"],["dc.rights","CC BY 2.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.subject.ddc","570"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Chromosome Mapping"],["dc.subject.mesh","Crosses, Genetic"],["dc.subject.mesh","DNA Transposable Elements"],["dc.subject.mesh","Embryo Loss"],["dc.subject.mesh","Embryo, Nonmammalian"],["dc.subject.mesh","Enhancer Elements, Genetic"],["dc.subject.mesh","Genes, Lethal"],["dc.subject.mesh","Genetic Markers"],["dc.subject.mesh","Germ-Line Mutation"],["dc.subject.mesh","Mutagenesis, Insertional"],["dc.subject.mesh","Transposases"],["dc.subject.mesh","Tribolium"],["dc.title","Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","53"],["dc.bibliographiccitation.journal","BMC Developmental Biology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Schinko, Johannes B."],["dc.contributor.author","Weber, Markus"],["dc.contributor.author","Viktorinova, Ivana"],["dc.contributor.author","Kiupakis, Alexandros"],["dc.contributor.author","Averof, Michalis"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T08:43:11Z"],["dc.date.available","2018-11-07T08:43:11Z"],["dc.date.issued","2010"],["dc.description.abstract","Background: The red flour beetle Tribolium castaneum has developed into an insect model system second only to Drosophila. Moreover, as a coleopteran it represents the most species-rich metazoan taxon which also includes many pest species. The genetic toolbox for Tribolium research has expanded in the past years but spatio-temporally controlled misexpression of genes has not been possible so far. Results: Here we report the establishment of the GAL4/UAS binary expression system in Tribolium castaneum. Both GAL4 Delta. and GAL4VP16 driven by the endogenous heat shock inducible promoter of the Tribolium hsp68 gene are efficient in activating reporter gene expression under the control of the Upstream Activating Sequence (UAS). UAS driven ubiquitous tGFP fluorescence was observed in embryos within four hours after activation while in-situ hybridization against tGFP revealed expression already after two hours. The response is quick in relation to the duration of embryonic development in Tribolium - 72 hours with segmentation being completed after 24 hours - which makes the study of early embryonic processes possible using this system. By comparing the efficiency of constructs based on Tribolium, Drosophila, and artificial core promoters, respectively, we find that the use of endogenous core promoters is essential for high-level expression of transgenic constructs. Conclusions: With the established GAL4/UAS binary expression system, ectopic misexpression approaches are now feasible in Tribolium. Our results support the contention that high-level transgene expression usually requires endogenous regulatory sequences, including endogenous core promoters in Tribolium and probably also other model systems."],["dc.identifier.doi","10.1186/1471-213X-10-53"],["dc.identifier.isi","000279816900001"],["dc.identifier.pmid","20482875"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5677"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19899"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-213X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Functionality of the GAL4/UAS system in Tribolium requires the use of endogenous core promoters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]