Bucher, Gregor

[["dc.bibliographiccitation.artnumber","25"],["dc.bibliographiccitation.journal","BMC Developmental Biology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Peel, Andrew D."],["dc.contributor.author","Schanda, Julia"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Ruge, Frank"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Gilles, Anna F."],["dc.contributor.author","Schinko, Johannes B."],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:23:38Z"],["dc.date.available","2018-11-07T09:23:38Z"],["dc.date.issued","2013"],["dc.description.abstract","Background: The Drosophila larval head is evolutionarily derived at the genetic and morphological level. In the beetle Tribolium castaneum, development of the larval head more closely resembles the ancestral arthropod condition. Unlike in Drosophila, a knirps homologue (Tc-kni) is required for development of the antennae and mandibles. However, published Tc-kni data are restricted to cuticle phenotypes and Tc-even-skipped and Tc-wingless stainings in knockdown embryos. Hence, it has remained unclear whether the entire antennal and mandibular segments depend on Tc-kni function, and whether the intervening intercalary segment is formed completely. We address these questions with a detailed examination of Tc-kni function. Results: By examining the expression of marker genes in RNAi embryos, we show that Tc-kni is required only for the formation of the posterior parts of the antennal and mandibular segments (i.e. the parasegmental boundaries). Moreover, we find that the role of Tc-kni is distinct in these segments: Tc-kni is required for the initiation of the antennal parasegment boundary, but only for the maintenance of the mandibular parasegmental boundary. Surprisingly, Tc-kni controls the timing of expression of the Hox gene Tc-labial in the intercalary segment, although this segment does form in the absence of Tc-kni function. Unexpectedly, we find that the pair-rule gene Tc-even-skipped helps set the posterior boundary of Tc-kni expression in the mandible. Using the mutant antennaless, a likely regulatory Null mutation at the Tc-kni locus, we provide evidence that our RNAi studies represent a Null situation. Conclusions: Tc-kni is required for the initiation of the antennal and the maintenance of the mandibular parasegmental boundaries. Tc-kni is not required for specification of the anterior regions of these segments, nor the intervening intercalary segment, confirming that Tc-kni is not a canonical 'gap-gene'. Our finding that a gap gene orthologue is regulated by a pair rule gene adds to the view that the segmentation gene hierarchies differ between Tribolium and Drosophila upstream of the pair rule gene level. In Tribolium, as in Drosophila, head and trunk segmentation gene networks cooperate to pattern the mandibular segment, albeit involving Tc-kni as novel component."],["dc.description.sponsorship","Deutsche Forschunggemeinschaft DFG [BU-1443/3-1]; DFG [Kl656/2]"],["dc.identifier.doi","10.1186/1471-213X-13-25"],["dc.identifier.isi","000321137000001"],["dc.identifier.pmid","23777260"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9126"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29627"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-213X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Tc-knirps plays different roles in the specification of antennal and mandibular parasegment boundaries and is regulated by a pair-rule gene in the beetle Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","219"],["dc.bibliographiccitation.lastpage","232"],["dc.bibliographiccitation.seriesnr","2047"],["dc.contributor.author","Buescher, Marita"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Garcia-Perez, Natalia Carolina"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.editor","Sprecher, Simon G."],["dc.date.accessioned","2021-06-02T10:44:27Z"],["dc.date.available","2021-06-02T10:44:27Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/978-1-4939-9732-9_12"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87047"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","Springer New York"],["dc.publisher.place","New York, NY"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.eisbn","978-1-4939-9732-9"],["dc.relation.isbn","978-1-4939-9731-2"],["dc.relation.ispartof","Methods in Molecular Biology"],["dc.relation.ispartof","Brain Development : Methods and Protocols"],["dc.relation.ispartofseries","Methods in Molecular Biology; 2047"],["dc.title","A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4740"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Development"],["dc.bibliographiccitation.lastpage","4750"],["dc.bibliographiccitation.volume","141"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Siemanowski, Janna L."],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:31:58Z"],["dc.date.available","2018-11-07T09:31:58Z"],["dc.date.issued","2014"],["dc.description.abstract","Wnt/beta-catenin and hedgehog (Hh) signaling are essential for transmitting signals across cell membranes in animal embryos. Early patterning of the principal insect model, Drosophila melanogaster, occurs in the syncytial blastoderm, where diffusion of transcription factors obviates the need for signaling pathways. However, in the cellularized growth zone of typical short germ insect embryos, signaling pathways are predicted to play a more fundamental role. Indeed, the Wnt/beta-catenin pathway is required for posterior elongation in most arthropods, although which target genes are activated in this context remains elusive. Here, we use the short germ beetle Tribolium castaneum to investigate two Wnt and Hh signaling centers located in the head anlagen and in the growth zone of early embryos. We find that Wnt/beta-catenin signaling acts upstream of Hh in the growth zone, whereas the opposite interaction occurs in the head. We determine the target gene sets of the Wnt/beta-catenin and Hh pathways and find that the growth zone signaling center activates a much greater number of genes and that the Wnt and Hh target gene sets are essentially non-overlapping. The Wnt pathway activates key genes of all three germ layers, including pair-rule genes, and Tc-caudal and Tc-twist. Furthermore, the Wnt pathway is required for hindgut development and we identify Tc-senseless as a novel hindgut patterning gene required in the early growth zone. At the same time, Wnt acts on growth zone metabolism and cell division, thereby integrating growth with patterning. Posterior Hh signaling activates several genes potentially involved in a proteinase cascade of unknown function."],["dc.identifier.doi","10.1242/dev.112797"],["dc.identifier.isi","346431900010"],["dc.identifier.pmid","25395458"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31644"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Company Of Biologists Ltd"],["dc.relation.issn","1477-9129"],["dc.relation.issn","0950-1991"],["dc.title","Wnt/beta-catenin signaling integrates patterning and metabolism of the insect growth zone"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7782"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","7786"],["dc.bibliographiccitation.volume","109"],["dc.contributor.author","Fu, Jinping"],["dc.contributor.author","Posnien, Nico"],["dc.contributor.author","Bolognesi, Renata"],["dc.contributor.author","Fischer, Tamara D."],["dc.contributor.author","Rayl, Parker"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Kitzmann, Peter"],["dc.contributor.author","Brown, Susan J."],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:10:25Z"],["dc.date.available","2018-11-07T09:10:25Z"],["dc.date.issued","2012"],["dc.description.abstract","Canonical Wnt signaling has been implicated in an AP axis polarizing mechanism in most animals, despite limited evidence from arthropods. In the long-germ insect, Drosophila, Wnt signaling is not required for global AP patterning, but in short-germ insects including Tribolium castaneum, loss of Wnt signaling affects development of segments in the growth zone but not those defined in the blastoderm. To determine the effects of ectopic Wnt signaling, we analyzed the expression and function of axin, which encodes a highly conserved negative regulator of the pathway. We found Tc-axin transcripts maternally localized to the anterior pole in freshly laid eggs. Expression spread toward the posterior pole during the early cleavage stages, becoming ubiquitous by the time the germ rudiment formed. Tc-axin RNAi produced progeny phenotypes that ranged from mildly affected embryos with cuticles displaying a graded loss of anterior structures, to defective embryos that condensed at the posterior pole in the absence of serosa. Altered expression domains of several blastodermal markers indicated anterior expansion of posterior fates. Analysis of other canonical Wnt pathway components and the expansion of Tc-caudal expression, a Wnt target, suggest that the effects of Tc-axin depletion are mediated through this pathway and that Wnt signaling must be inhibited for proper anterior development in Tribolium. These studies provide unique evidence that canonical Wnt signaling must be carefully regulated along the AP axis in an arthropod, and support an ancestral role for Wnt activity in defining AP polarity and patterning in metazoan development."],["dc.identifier.doi","10.1073/pnas.1116641109"],["dc.identifier.isi","000304369800049"],["dc.identifier.pmid","22552230"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26486"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","Asymmetrically expressed axin required for anterior development in Tribolium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]