Bucher, Gregor

[["dc.bibliographiccitation.firstpage","D720"],["dc.bibliographiccitation.issue","D1"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.lastpage","D725"],["dc.bibliographiccitation.volume","43"],["dc.contributor.author","Doenitz, Juergen"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Gerischer, Lizzy"],["dc.contributor.author","Tech, Maike"],["dc.contributor.author","Schoppmeier, Michael"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T10:01:53Z"],["dc.date.available","2018-11-07T10:01:53Z"],["dc.date.issued","2015"],["dc.description.abstract","The iBeetle-Base (http://ibeetle-base.uni-goettingen.de) makes available annotations of RNAi phenotypes, which were gathered in a large scale RNAi screen in the red flour beetle Tribolium castaneum (iBeetle screen). In addition, it provides access to sequence information and links for all Tribolium cas-taneum genes. The iBeetle-Base contains the annotations of phenotypes of several thousands of genes knocked down during embryonic and metamorphic epidermis and muscle development in addition to phenotypes linked to oogenesis and stink gland biology. The phenotypes are described according to the EQM (entity, quality, modifier) system using controlled vocabularies and the Tribolium morphological ontology (TrOn). Furthermore, images linked to the respective annotations are provided. The data are searchable either for specific phenotypes using a complex 'search for morphological defects' or a 'quick search' for gene names and IDs. The red flour beetle Tribolium castaneum has become an important model system for insect functional genetics and is a representative of the most species rich taxon, the Coleoptera, which comprise several devastating pests. It is used for studying insect typical development, the evolution of development and for research on metabolism and pest control. Besides Drosophila, Tribolium is the first insect model organism where large scale unbiased screens have been performed."],["dc.description.sponsorship","Open-Access-Publikationsfunds 2014"],["dc.identifier.doi","10.1093/nar/gku1054"],["dc.identifier.isi","000350210400105"],["dc.identifier.pmid","25378303"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11085"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38124"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1362-4962"],["dc.relation.issn","0305-1048"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","5"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Kitzmann, Peter"],["dc.contributor.author","Schwirz, Jonas"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:29:08Z"],["dc.date.available","2018-11-07T09:29:08Z"],["dc.date.issued","2013"],["dc.description.abstract","Background: RNA interference (RNAi) is a powerful tool to study gene function in organisms that are not amenable to classical forward genetics. Hence, together with the ease of comprehensively identifying genes by new generation sequencing, RNAi is expanding the scope of animal species and questions that can be addressed in terms of gene function. In the case of genetic mutants, the genetic background of the strains used is known to influence the phenotype while this has not been described for RNAi experiments. Results: Here we show in the red flour beetle Tribolium castaneum that RNAi against Tc-importin alpha 1 leads to different phenotypes depending on the injected strain. We rule out off target effects and show that sequence divergence does not account for this difference. By quantitatively comparing phenotypes elicited by RNAi knockdown of four different genes we show that there is no general difference in RNAi sensitivity between these strains. Finally, we show that in case of Tc-importin a1 the difference depends on the maternal genotype. Conclusions: These results show that in RNAi experiments strain specific differences have to be considered and that a proper documentation of the injected strain is required. This is especially important for the increasing number of emerging model organisms that are being functionally investigated using RNAi. In addition, our work shows that RNAi is suitable to systematically identify the differences in the gene regulatory networks present in populations of the same species, which will allow novel insights into the evolution of animal diversity."],["dc.description.sponsorship","DFG [FOR1234]"],["dc.identifier.doi","10.1186/1471-2164-14-5"],["dc.identifier.isi","000315034000001"],["dc.identifier.pmid","23324472"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8539"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30946"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","RNAi phenotypes are influenced by the genetic background of the injected strain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","608"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Lehmann, Sabrina"],["dc.contributor.author","Atika, Bibi"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Strohlein, Nadi"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Weißkopf, Matthias"],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Teuscher, Matthias"],["dc.contributor.author","Hakeemi, Muhammad S."],["dc.contributor.author","Li, Jianwei"],["dc.contributor.author","Weißbecker, Bernhard"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2022-08-22T06:20:44Z"],["dc.date.available","2022-08-22T06:20:44Z"],["dc.date.issued","2022-08-20"],["dc.date.updated","2022-08-21T03:10:50Z"],["dc.description.abstract","Abstract\n \n Background\n Functional genomics uses unbiased systematic genome-wide gene disruption or analyzes natural variations such as gene expression profiles of different tissues from multicellular organisms to link gene functions to particular phenotypes. Functional genomics approaches are of particular importance to identify large sets of genes that are specifically important for a particular biological process beyond known candidate genes, or when the process has not been studied with genetic methods before.\n \n \n Results\n Here, we present a large set of genes whose disruption interferes with the function of the odoriferous defensive stink glands of the red flour beetle Tribolium castaneum. This gene set is the result of a large-scale systematic phenotypic screen using RNA interference applied in a genome-wide forward genetics manner. In this first-pass screen, 130 genes were identified, of which 69 genes could be confirmed to cause phenotypic changes in the glands upon knock-down, which vary from necrotic tissue and irregular reservoir size to irregular color or separation of the secreted gland compounds. Gene ontology analysis revealed that many of those genes are encoding enzymes (peptidases and cytochromes P450) as well as proteins involved in membrane trafficking with an enrichment in lysosome and mineral absorption pathways. The knock-down of 13 genes caused specifically a strong reduction of para-benzoquinones in the gland reservoirs, suggesting a specific function in the synthesis of these toxic compounds. Only 14 of the 69 confirmed gland genes are differentially overexpressed in stink gland tissue and thus could have been detected in a transcriptome-based analysis. However, only one out of eight genes identified by a transcriptomics approach known to cause phenotypic changes of the glands upon knock-down was recognized by this phenotypic screen, indicating the limitation of such a non-redundant first-pass screen.\n \n \n Conclusion\n Our results indicate the importance of combining diverse and independent methodologies to identify genes necessary for the function of a certain biological tissue, as the different approaches do not deliver redundant results but rather complement each other. The presented phenotypic screen together with a transcriptomics approach are now providing a set of close to hundred genes important for odoriferous defensive stink gland physiology in beetles."],["dc.identifier.citation","BMC Genomics. 2022 Aug 20;23(1):608"],["dc.identifier.doi","10.1186/s12864-022-08822-z"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113084"],["dc.language.iso","en"],["dc.publisher","BioMed Central"],["dc.rights.holder","The Author(s)"],["dc.subject","Chemical ecology"],["dc.subject","Genome-wide"],["dc.subject","iBeetle"],["dc.subject","Odoriferous glands"],["dc.subject","RNA interference"],["dc.subject","RNAseq Tribolium castaneum"],["dc.title","Phenotypic screen and transcriptomics approach complement each other in functional genomics of defensive stink gland physiology"],["dc.type","journal_article"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","7822"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Schultheis, Dorothea"],["dc.contributor.author","Schwirz, Jonas"],["dc.contributor.author","Stroehlein, Nadi"],["dc.contributor.author","Troelenberg, Nicole"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Dao, Van Anh"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Tech, Maike"],["dc.contributor.author","Doenitz, Juergen"],["dc.contributor.author","Gerischer, Lizzy"],["dc.contributor.author","Theis, Mirko"],["dc.contributor.author","Schild, Inga"],["dc.contributor.author","Trauner, Jochen"],["dc.contributor.author","Koniszewski, Nikolaus Dieter Bernhard"],["dc.contributor.author","Kuester, Elke"],["dc.contributor.author","Kittelmann, Sebastian"],["dc.contributor.author","Hu, Yonggang"],["dc.contributor.author","Lehmann, Sabrina"],["dc.contributor.author","Siemanowski, Janna L."],["dc.contributor.author","Ulrich, Julia"],["dc.contributor.author","Panfilio, Kristen A."],["dc.contributor.author","Schroeder, Reinhard"],["dc.contributor.author","Morgenstern, Burkhard"],["dc.contributor.author","Stanke, Mario"],["dc.contributor.author","Buchhholz, Frank"],["dc.contributor.author","Frasch, Manfred"],["dc.contributor.author","Roth, Siegfried"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Schoppmeier, Michael"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:55:00Z"],["dc.date.available","2018-11-07T09:55:00Z"],["dc.date.issued","2015"],["dc.description.abstract","Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila."],["dc.description.sponsorship","Open-Access Publikationsfonds 2015"],["dc.identifier.doi","10.1038/ncomms8822"],["dc.identifier.isi","000358860900002"],["dc.identifier.pmid","26215380"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12460"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36659"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","20181373"],["dc.bibliographiccitation.issue","1885"],["dc.bibliographiccitation.journal","Proceedings of the Royal Society of London. Series B, Biological Sciences"],["dc.bibliographiccitation.volume","285"],["dc.contributor.author","Hu, Yonggang"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Schwirz, Jonas"],["dc.contributor.author","Stroehlein, Nadi"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2020-12-10T18:20:17Z"],["dc.date.available","2020-12-10T18:20:17Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1098/rspb.2018.1373"],["dc.identifier.eissn","1471-2954"],["dc.identifier.issn","0962-8452"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75512"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","A morphological novelty evolved by co-option of a reduced gene regulatory network and gene recruitment in a beetle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e70695"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Dönitz, Jürgen"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Schild, Inga"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Bradler, Sven"],["dc.contributor.author","Prpic, Nikola-Michael"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2019-07-09T11:54:30Z"],["dc.date.available","2019-07-09T11:54:30Z"],["dc.date.issued","2013-07-30"],["dc.description.abstract","In a morphological ontology the expert’s knowledge is represented in terms, which describe morphological structures and how these structures relate to each other. With the assistance of ontologies this expert knowledge is made processable by machines, through a formal and standardized representation of terms and their relations to each other. The red flour beetle Tribolium castaneum, a representative of the most species rich animal taxon on earth (the Coleoptera), is an emerging model organism for development, evolution, physiology, and pest control. In order to foster Tribolium research, we have initiated the Tribolium Ontology (TrOn), which describes the morphology of the red flour beetle. The content of this ontology comprises so far most external morphological structures as well as some internal ones. All modeled structures are consistently annotated for the developmental stages larva, pupa and adult. In TrOn all terms are grouped into three categories: Generic terms represent morphological structures, which are independent of a developmental stage. In contrast, downstream of such terms are concrete terms which stand for a dissectible structure of a beetle at a specific life stage. Finally, there are mixed terms describing structures that are only found at one developmental stage. These terms combine the characteristics of generic and concrete terms with features of both. These annotation principles take into account the changing morphology of the beetle during development and provide generic terms to be used in applications or for cross linking with other ontologies and data resources. We use the ontology for implementing an intuitive search function at the electronic iBeetle-Base, which stores morphological defects found in a genome wide RNA interference (RNAi) screen. The ontology is available for download at http://ibeetle-base.uni-goettingen.de."],["dc.format.extent","8"],["dc.identifier.doi","10.1371/journal.pone.0070695"],["dc.identifier.fs","603116"],["dc.identifier.pmid","23936240"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9209"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60669"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","TrOn: An Anatomical Ontology for the Beetle Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","674"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Ulrich, Julia"],["dc.contributor.author","Dao, Van Anh"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Schwirz, Jonas"],["dc.contributor.author","Schultheis, Dorothea"],["dc.contributor.author","Stroehlein, Nadi"],["dc.contributor.author","Troelenberg, Nicole"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Doenitz, Juergen"],["dc.contributor.author","Gerischer, Lizzy"],["dc.contributor.author","Leboulle, Gerard"],["dc.contributor.author","Vilcinskas, Andreas"],["dc.contributor.author","Stanke, Mario"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2018-11-07T09:51:47Z"],["dc.date.available","2018-11-07T09:51:47Z"],["dc.date.issued","2015"],["dc.description.abstract","Background: Insect pest control is challenged by insecticide resistance and negative impact on ecology and health. One promising pest specific alternative is the generation of transgenic plants, which express double stranded RNAs targeting essential genes of a pest species. Upon feeding, the dsRNA induces gene silencing in the pest resulting in its death. However, the identification of efficient RNAi target genes remains a major challenge as genomic tools and breeding capacity is limited in most pest insects impeding whole-animal-high-throughput-screening. Results: We use the red flour beetle Tribolium castaneum as a screening platform in order to identify the most efficient RNAi target genes. From about 5,000 randomly screened genes of the iBeetle RNAi screen we identify 11 novel and highly efficient RNAi targets. Our data allowed us to determine GO term combinations that are predictive for efficient RNAi target genes with proteasomal genes being most predictive. Finally, we show that RNAi target genes do not appear to act synergistically and that protein sequence conservation does not correlate with the number of potential off target sites. Conclusions: Our results will aid the identification of RNAi target genes in many pest species by providing a manageable number of excellent candidate genes to be tested and the proteasome as prime target. Further, the identified GO term combinations will help to identify efficient target genes from organ specific transcriptomes. Our off target analysis is relevant for the sequence selection used in transgenic plants."],["dc.description.sponsorship","German Research Foundation (DFG) [FOR1234]; Gottingen University"],["dc.identifier.doi","10.1186/s12864-015-1880-y"],["dc.identifier.isi","000360607100014"],["dc.identifier.pmid","26334912"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13452"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35981"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","g3.200995.2018"],["dc.bibliographiccitation.journal","G3: Genes, Genomes, Genetics"],["dc.contributor.author","Schultheis, Dorothea"],["dc.contributor.author","Weißkopf, Matthias"],["dc.contributor.author","Schaub, Christoph"],["dc.contributor.author","Ansari, Salim"],["dc.contributor.author","Dao, Van Anh"],["dc.contributor.author","Grossmann, Daniela"],["dc.contributor.author","Majumdar, Upalparna"],["dc.contributor.author","Hakeemi, Muhammad Salim"],["dc.contributor.author","Troelenberg, Nicole"],["dc.contributor.author","Richter, Tobias"],["dc.contributor.author","Schmitt-Engel, Christian"],["dc.contributor.author","Schwirz, Jonas"],["dc.contributor.author","Ströhlein, Nadia"],["dc.contributor.author","Teuscher, Matthias"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Frasch, Manfred"],["dc.date.accessioned","2020-12-10T18:42:40Z"],["dc.date.available","2020-12-10T18:42:40Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1534/g3.118.200995"],["dc.identifier.eissn","2160-1836"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78042"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","A Large Scale Systemic RNAi Screen in the Red Flour Beetle Tribolium castaneum Identifies Novel Genes Involved in Insect Muscle Development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]