Bucher, Gregor

[["dc.bibliographiccitation.artnumber","60"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Frontiers in Zoology"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Mehlhorn, Sonja"],["dc.contributor.author","Hunnekuhl, Vera S."],["dc.contributor.author","Geibel, Sven"],["dc.contributor.author","Nauen, Ralf"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2022-01-11T14:07:53Z"],["dc.date.accessioned","2022-08-18T12:37:06Z"],["dc.date.available","2022-01-11T14:07:53Z"],["dc.date.available","2022-08-18T12:37:06Z"],["dc.date.issued","2021-12-04"],["dc.date.updated","2022-07-29T12:17:37Z"],["dc.description.abstract","RNA interference (RNAi) has emerged as a powerful tool for knocking-down gene function in diverse taxa including arthropods for both basic biological research and application in pest control. The conservation of the RNAi mechanism in eukaryotes suggested that it should—in principle—be applicable to most arthropods. However, practical hurdles have been limiting the application in many taxa. For instance, species differ considerably with respect to efficiency of dsRNA uptake from the hemolymph or the gut. Here, we review some of the most frequently encountered technical obstacles when establishing RNAi and suggest a robust procedure for establishing this technique in insect species with special reference to pests. Finally, we present an approach to identify the most effective target genes for the potential control of agricultural and public health pests by RNAi."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.citation","Frontiers in Zoology. 2021 Dec 04;18(1):60"],["dc.identifier.doi","10.1186/s12983-021-00444-7"],["dc.identifier.pii","444"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97884"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112959"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.publisher","BioMed Central"],["dc.relation.eissn","1742-9994"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.subject","RNA interference"],["dc.subject","RNAi"],["dc.subject","Establishment of RNAi"],["dc.subject","Off target control"],["dc.subject","Controls"],["dc.subject","Variability"],["dc.subject","Pest control"],["dc.title","Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","14"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","EvoDevo"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.date.accessioned","2022-08-04T11:57:52Z"],["dc.date.available","2022-08-04T11:57:52Z"],["dc.date.issued","2022-07-19"],["dc.date.updated","2022-07-25T11:18:58Z"],["dc.description.abstract","The red flour beetle Tribolium castaneum has emerged as an important insect model system for a variety of topics. With respect to studying gene function, it is second only to the vinegar fly D. melanogaster. The RNAi response in T. castaneum is exceptionally strong and systemic, and it appears to target all cell types and processes. Uniquely for emerging model organisms, T. castaneum offers the opportunity of performing time- and cost-efficient large-scale RNAi screening, based on commercially available dsRNAs targeting all genes, which are simply injected into the body cavity. Well established transgenic and genome editing approaches are met by ease of husbandry and a relatively short generation time. Consequently, a number of transgenic tools like UAS/Gal4, Cre/Lox, imaging lines and enhancer trap lines are already available. T. castaneum has been a genetic experimental system for decades and now has become a workhorse for molecular and reverse genetics as well as in vivo imaging. Many aspects of development and general biology are more insect-typical in this beetle compared to D. melanogaster. Thus, studying beetle orthologs of well-described fly genes has allowed macro-evolutionary comparisons in developmental processes such as axis formation, body segmentation, and appendage, head and brain development. Transgenic approaches have opened new ways for in vivo imaging. Moreover, this emerging model system is the first choice for research on processes that are not represented in the fly, or are difficult to study there, e.g. extraembryonic tissues, cryptonephridial organs, stink gland function, or dsRNA-based pesticides."],["dc.identifier.citation","EvoDevo. 2022 Jul 19;13(1):14"],["dc.identifier.doi","10.1186/s13227-022-00201-9"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112640"],["dc.language.iso","en"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.subject","Insect model system"],["dc.subject","Arthropod model system"],["dc.subject","Coleoptera"],["dc.subject","Evolution of development"],["dc.subject","Physiology"],["dc.subject","Behavior"],["dc.subject","Pest control"],["dc.subject","Systemic RNAi"],["dc.subject","Genome-wide screen"],["dc.subject","CRISPR genome editing"],["dc.title","The red flour beetle T. castaneum: elaborate genetic toolkit and unbiased large scale RNAi screening to study insect biology and evolution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]