Knabe, Wolfgang

[["dc.bibliographiccitation.firstpage","719"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Neuroscience"],["dc.bibliographiccitation.lastpage","723"],["dc.contributor.author","Riechers, Claas-Christian"],["dc.contributor.author","Knabe, Wolfgang"],["dc.contributor.author","Sirén, Anna-Leena"],["dc.contributor.author","Gariepy, C. E."],["dc.contributor.author","Yanagisawa, M."],["dc.contributor.author","Ehrenreich, Hannelore"],["dc.date.accessioned","2017-09-07T11:45:48Z"],["dc.date.available","2017-09-07T11:45:48Z"],["dc.date.issued","2004"],["dc.identifier.gro","3150462"],["dc.identifier.pmid","15026112"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7228"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.relation.issn","0306-4522"],["dc.title","Endothelin B receptor deficient transgenic rescue rats: A rescue phenomenon in the brain"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","503"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","ANATOMY AND EMBRYOLOGY"],["dc.bibliographiccitation.lastpage","512"],["dc.bibliographiccitation.volume","207"],["dc.contributor.author","Knabe, Wolfgang"],["dc.contributor.author","Knerlich, F."],["dc.contributor.author","Washausen, Stefan"],["dc.contributor.author","Kietzmann, Thomas"],["dc.contributor.author","Siren, A. L."],["dc.contributor.author","Brunnett, G."],["dc.contributor.author","Kuhn, H. J."],["dc.contributor.author","Ehrenreich, Hannelore"],["dc.date.accessioned","2018-11-07T10:50:26Z"],["dc.date.available","2018-11-07T10:50:26Z"],["dc.date.issued","2004"],["dc.description.abstract","The expression patterns of erythropoietin (EPO) and its receptor (EPOR) were investigated in the midbrain and in adjacent parts of the synencephalon and hindbrain of embryonic C57Bl mice. On embryonic (E) day 8 (E8), virtually all neuroepithelial cells expressed EPOR. After neural tube closure, subsets of these cells downregulated EPOR. In contrast, radial glial cells were EPOR-immunolabeled from E11 onwards. Simultaneously, subpopulations of early developing neurons upregulated EPO and expressed HIF-1, known to transcriptionally activate EPO. Three-dimensional reconstructions revealed subpopulations of EPO-expressing neurons: (1) in the trigeminal mesencephalic nucleus (TMN), (2) at the rostral transition of the midbrain and synencephalon, (3) in the basal plate of the midbrain, (4) in the trigeminal motor nucleus, and (5) in the trigeminal principal sensory nucleus. In the rostral midbrain and synencephalon, EPO-immunoreactive neurons were attached to EPOR-expressing radial glial cells. The identity of radial glial cells was proven by their immunoreactivity for antibodies against astrocyte-specific glutamate transporter, brain lipid-binding protein, and nestin. From E12.5 onwards EPOR was downregulated in radial glial cells. Viable neurons of the TMN continued to express EPO and upregulated EPOR. Our findings provide new evidence that components of the EPO system are present in distinct locations of the embryonic brain and, by interactions between neurons and radial glial cells as well as among clustered TMN neurons, may contribute to its morphogenesis. Whether the observed expression patterns of EPO and EPOR may reflect EPO-mediated trophic and/or antiapoptotic effects on neurons is discussed."],["dc.identifier.doi","10.1007/s00429-003-0365-y"],["dc.identifier.isi","000220086500009"],["dc.identifier.pmid","14770308"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48650"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0340-2061"],["dc.title","Expression patterns of erythropoietin and its receptor in the developing midbrain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","209"],["dc.bibliographiccitation.journal","Anatomy and Embryology"],["dc.bibliographiccitation.lastpage","219"],["dc.bibliographiccitation.volume","210"],["dc.contributor.author","Knabe, Wolfgang"],["dc.contributor.author","Sirén, Anna-Leena"],["dc.contributor.author","Ehrenreich, Hannelore"],["dc.contributor.author","Kuhn, Hans-Jürg"],["dc.date.accessioned","2017-09-07T11:45:44Z"],["dc.date.available","2017-09-07T11:45:44Z"],["dc.date.issued","2005"],["dc.description.abstract","Recombinant human erythropoietin (EPO) is neuroprotective in animal models of adult spinal cord injury, and reduces apoptosis in adult dorsal root ganglia after spinal nerve crush. The present work demonstrates that spinal cord and dorsal root ganglia share dynamic expression patterns of EPO and its receptor (EPOR) during development. C57Bl mice from embryonic days (E) 8 (E8) to E19 were studied. In spinal cord and dorsal root ganglia, EPOR expression in all precursor cells preceded the expression of EPO in subsets of neurons. On E11, EPO-immunoreactive spinal motoneurons and ganglionic sensory neurons resided adjacent to EPOR-expressing radial glial cells and satellite cells, respectively. From E12 onwards, EPOR-immunoreactivity decreased in radial glial cells and, transiently, in satellite cells. Simultaneously, large-scale apoptosis of motoneurons and sensory neurons started, and subsets of neurons were labelled by antibodies against EPOR. Viable neurons expressed EPO and EPOR. Up to E12.5, apoptotic cells were EPOR-immunopositive, but variably EPO-immunonegative or EPO-immunopositive. Thereafter, EPO-immunonegative and EPOR-immunopositive apoptotic cells predominated. Our findings suggest that EPO-mediated neuron-glial and, later, neuron-neuronal interactions promote the differentiation and/or the survival of subsets of neurons and glial cells in central as well as in peripheral parts of the embryonic nervous system. Correspondingly, expression of phospho-Akt-1/protein-kinase B extensively overlapped expression sites of EPO and EPOR, but was absent from apoptotic cells. Identified other sites of EPO and/or EPOR expression include radial glial cells that transform to astrocytes, cells of the floor plate and notochord as well as neural crest-derived boundary cap cells at motor exit points and cells of the primary sympathetic chain."],["dc.identifier.doi","10.1007/s00429-005-0019-3"],["dc.identifier.gro","3150443"],["dc.identifier.pmid","16151855"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7207"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.subject","Erythropoietin; Spinal cord; Dorsal root ganglia; Mouse embryo; Apoptosis"],["dc.title","Expression patterns of erythropoietin and its receptor in the developing spinal cord and dorsal root ganglia"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]