Menck, Kerstin

[["dc.bibliographiccitation.firstpage","285"],["dc.bibliographiccitation.journal","Oncology Research and Treatment"],["dc.bibliographiccitation.lastpage","286"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Bleckmann, Annalen"],["dc.contributor.author","Scharf, Christian"],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Dyck, Lydia"],["dc.contributor.author","Klemm, Florian"],["dc.contributor.author","Binder, Claudia"],["dc.date.accessioned","2018-11-07T09:34:18Z"],["dc.date.available","2018-11-07T09:34:18Z"],["dc.date.issued","2014"],["dc.identifier.isi","000343816900702"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32144"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.relation.issn","2296-5262"],["dc.relation.issn","2296-5270"],["dc.title","EMMPRIN/CD147-positive tumor cell microvesicles are pro-invasive and detectable in the blood of cancer patients with metastasis"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","142"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Heinrichs, Saskia"],["dc.contributor.author","Baden, Cornelia"],["dc.contributor.author","Bleckmann, Annalen"],["dc.date.accessioned","2021-04-14T08:29:45Z"],["dc.date.available","2021-04-14T08:29:45Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.3390/cells10010142"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82982"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2073-4409"],["dc.title","The WNT/ROR Pathway in Cancer: From Signaling to Therapeutic Intervention"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Onkologie"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Schulz, M."],["dc.contributor.author","Dyck, Lydia"],["dc.contributor.author","Binder, Claudia"],["dc.contributor.author","Klemm, Florian"],["dc.date.accessioned","2018-11-07T09:04:54Z"],["dc.date.available","2018-11-07T09:04:54Z"],["dc.date.issued","2012"],["dc.format.extent","195"],["dc.identifier.isi","000310766700508"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25205"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.relation.issn","0378-584X"],["dc.title","Breast cancer invasion mediated by plasma membrane-derived microvesicles is EMMPRIN-dependent"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Onkologie"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Bleckmann, Annalen"],["dc.contributor.author","Leha, Andreas"],["dc.contributor.author","Artmann, Stephan"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Binder, Claudia"],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Klemm, E."],["dc.date.accessioned","2018-11-07T09:19:06Z"],["dc.date.available","2018-11-07T09:19:06Z"],["dc.date.issued","2013"],["dc.identifier.isi","000326360900431"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28557"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.title","Identification of prognostic miRNAs in breast cancer through profiling of tumor educated macrophages"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Onkologie"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Klemm, Florian"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Schulz, M."],["dc.contributor.author","Binder, Claudia"],["dc.date.accessioned","2018-11-07T08:38:50Z"],["dc.date.available","2018-11-07T08:38:50Z"],["dc.date.issued","2010"],["dc.format.extent","199"],["dc.identifier.isi","000282988401115"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18852"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.relation.issn","0378-584X"],["dc.title","Tumor-Microparticles mediate invasiveness and elicit a M2-response in macrophages"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e51554"],["dc.bibliographiccitation.issue","91"],["dc.bibliographiccitation.journal","Journal of Visualized Experiments"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Behme, Daniel"],["dc.contributor.author","Pantke, Mathias"],["dc.contributor.author","Reiling, Norbert"],["dc.contributor.author","Binder, Claudia"],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Klemm, Florian"],["dc.date.accessioned","2018-11-07T09:35:29Z"],["dc.date.available","2018-11-07T09:35:29Z"],["dc.date.issued","2014"],["dc.description.abstract","Human macrophages are involved in a plethora of pathologic processes ranging from infectious diseases to cancer. Thus they pose a valuable tool to understand the underlying mechanisms of these diseases. We therefore present a straightforward protocol for the isolation of human monocytes from buffy coats, followed by a differentiation procedure which results in high macrophage yields. The technique relies mostly on commonly available lab equipment and thus provides a cost and time effective way to obtain large quantities of human macrophages. Briefly, buffy coats from healthy blood donors are subjected to a double density gradient centrifugation to harvest monocytes from the peripheral blood. These monocytes are then cultured in fluorinated ethylene propylene (FEP) Teflon-coated cell culture bags in the presence of macrophage colony-stimulating factor (M-CSF). The differentiated macrophages can be easily harvested and used for subsequent studies and functional assays. Important methods for quality control and validation of the isolation and differentiation steps will be highlighted within the protocol. In summary, the protocol described here enables scientists to routinely and reproducibly isolate human macrophages without the need for cost intensive tools. Furthermore, disease models can be studied in a syngeneic human system circumventing the use of murine macrophages."],["dc.identifier.doi","10.3791/51554"],["dc.identifier.isi","000349301100015"],["dc.identifier.pmid","25226391"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32396"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Journal Of Visualized Experiments"],["dc.relation.issn","1940-087X"],["dc.title","Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","225"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Muscle & Nerve"],["dc.bibliographiccitation.lastpage","230"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Rostasy, Kevin"],["dc.contributor.author","Piepkorn, M."],["dc.contributor.author","Goebel, Hans H."],["dc.contributor.author","Menck, S."],["dc.contributor.author","Hanefeld, Folker"],["dc.contributor.author","Schulz-Schaeffer, Walter J."],["dc.date.accessioned","2018-11-07T10:46:58Z"],["dc.date.available","2018-11-07T10:46:58Z"],["dc.date.issued","2004"],["dc.description.abstract","Recent advances have revealed significant differences in the pathogenesis of inflammatory myopathies. To determine whether different patterns of macrophage differentiation are a useful tool to delineate the major groups of inflammatory myopathies, the muscle biopsies of 11 patients with dermatomyositis and 12 patients with polymyositis were studied using different macrophage markers. In polymyositis, the early-activation markers MRP14 and 27E10 stained the majority of macrophages, which were recognized by the pan-macrophage marker Ki-M1P and which were located primarily in the endomysium. In dermatomyositis, macrophages predominantly expressed the late-activation marker 25F9 and were found mainly in the perimysium. Thus, the location and presence of different subsets of macrophages distinguish dermatomyositis and polymyositis. The predominance of early-activated macrophages in polymyositis indicates a more acute disease process. The findings in dermatomyositis, by contrast, suggest a role of persistent monocytes/macrophages in the disease process."],["dc.identifier.doi","10.1002/mus.20088"],["dc.identifier.isi","000222988600011"],["dc.identifier.pmid","15266639"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47858"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","John Wiley & Sons Inc"],["dc.relation.issn","0148-639X"],["dc.title","Monocyte/macrophage differentiation in dermatomyositis and polymyositis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Onkologie"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Klemm, Florian"],["dc.contributor.author","Schulz, M."],["dc.contributor.author","Pukrop, Tobias"],["dc.contributor.author","Binder, Claudia"],["dc.date.accessioned","2018-11-07T08:52:16Z"],["dc.date.available","2018-11-07T08:52:16Z"],["dc.date.issued","2011"],["dc.format.extent","60"],["dc.identifier.isi","000295160600152"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22127"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.relation.issn","0378-584X"],["dc.title","Identification and further characterization of microparticle populations in microparticle-induced breast cancer invasion"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e55057"],["dc.bibliographiccitation.issue","119"],["dc.bibliographiccitation.journal","Journal of Visualized Experiments"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Bleckmann, Annalen"],["dc.contributor.author","Schulz, Matthias"],["dc.contributor.author","Ries, Lena"],["dc.contributor.author","Binder, Claudia"],["dc.date.accessioned","2018-11-07T10:28:42Z"],["dc.date.available","2018-11-07T10:28:42Z"],["dc.date.issued","2017"],["dc.description.abstract","The release of extracellular vesicles (EVs) including small endosomal-derived exosomes (Exos, diameter < 100 nm) and large plasma membrane-derived microvesicles (MVs, diameter > 100 nm) is a fundamental cellular process that occurs in all living cells. These vesicles transport proteins, lipids and nucleic acids specific for their cell of origin and in vitro studies have highlighted their importance as mediators of intercellular communication. EVs have been successfully isolated from various body fluids and especially EVs in blood have been identified as promising biomarkers for cancer or infectious diseases. In order to allow the study of MV subpopulations in blood, we present a protocol for the standardized isolation and characterization of MVs from peripheral blood samples. MVs are pelleted from EDTA-anticoagulated plasma samples by differential centrifugation and typically possess a diameter of 100 - 600 nm. Due to their larger size, they can easily be studied by flow cytometry, a technique that is routinely used in clinical diagnostics and available in most laboratories. Several examples for quality control assays of the isolated MVs will be given and markers that can be used for the discrimination of different MV subpopulations in blood will be presented."],["dc.identifier.doi","10.3791/55057"],["dc.identifier.isi","000397847200045"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43486"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Journal Of Visualized Experiments"],["dc.relation.issn","1940-087X"],["dc.title","Isolation and Characterization of Microvesicles from Peripheral Blood"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5373"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Menck, Kerstin"],["dc.contributor.author","Sivaloganathan, Suganja"],["dc.contributor.author","Bleckmann, Annalen"],["dc.contributor.author","Binder, Claudia"],["dc.date.accessioned","2021-04-14T08:23:47Z"],["dc.date.available","2021-04-14T08:23:47Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.3390/ijms21155373"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81045"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1422-0067"],["dc.title","Microvesicles in Cancer: Small Size, Large Potential"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]