Chernev, Aleksandar

[["dc.bibliographiccitation.firstpage","1082"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Cell"],["dc.bibliographiccitation.lastpage","1093"],["dc.bibliographiccitation.volume","171"],["dc.contributor.author","Hillen, Hauke S."],["dc.contributor.author","Parshin, Andrey V."],["dc.contributor.author","Agaronyan, Karen"],["dc.contributor.author","Morozov, Yaroslav I."],["dc.contributor.author","Graber, James J."],["dc.contributor.author","Chernev, Aleksandar"],["dc.contributor.author","Schwinghammer, Kathrin"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Anikin, Michael"],["dc.contributor.author","Cramer, Patrick"],["dc.contributor.author","Temiakov, Dmitry"],["dc.date.accessioned","2018-01-09T12:26:24Z"],["dc.date.available","2018-01-09T12:26:24Z"],["dc.date.issued","2017"],["dc.description.abstract","In human mitochondria, transcription termination events at a G-quadruplex region near the replication origin are thought to drive replication of mtDNA by generation of an RNA primer. This process is suppressed by a key regulator of mtDNA-the transcription factor TEFM. We determined the structure of an anti-termination complex in which TEFM is bound to transcribing mtRNAP. The structure reveals interactions of the dimeric pseudonuclease core of TEFM with mobile structural elements in mtRNAP and the nucleic acid components of the elongation complex (EC). Binding of TEFM to the DNA forms a downstream \"sliding clamp,\" providing high processivity to the EC. TEFM also binds near the RNA exit channel to prevent formation of the RNA G-quadruplex structure required for termination and thus synthesis of the replication primer. Our data provide insights into target specificity of TEFM and mechanisms by which it regulates the switch between transcription and replication of mtDNA."],["dc.identifier.doi","10.1016/j.cell.2017.09.035"],["dc.identifier.pmid","29033127"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11586"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1097-4172"],["dc.title","Mechanism of Transcription Anti-termination in Human Mitochondria"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3441"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Journal of Proteome Research"],["dc.bibliographiccitation.lastpage","3448"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Veit, Johannes"],["dc.contributor.author","Sachsenberg, Timo"],["dc.contributor.author","Chernev, Aleksandar"],["dc.contributor.author","Aicheler, Fabian"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Kohlbacher, Oliver"],["dc.date.accessioned","2018-11-07T10:09:48Z"],["dc.date.available","2018-11-07T10:09:48Z"],["dc.date.issued","2016"],["dc.description.abstract","Modern mass spectrometry setups used in today's proteomics studies generate vast amounts of raw data, calling for highly efficient data processing and analysis tools. Software for analyzing these data is either monolithic (easy to use, but sometimes too rigid) or workflow-driven (easy to customize, but sometimes complex). Thermo Proteome Discoverer (PD) is a powerful software for workflow-driven data analysis in proteomics which, in our eyes, achieves a good trade-off between flexibility and usability. Here, we present two open-source plugins for PD providing additional functionality: LFQProfiler for label-free quantification of peptides and proteins, and RNxl for UV-induced peptide RNA cross-linking data analysis. LFQProfiler interacts with existing PD nodes for peptide identification and validation and takes care of the entire quantitative part of the workflow. We show that it performs at least on par with other state-of-the-art software solutions for label-free quantification in a recently published benchmark (Ramus, C.; et al. J. Proteomics 2016, 132, 51-62). The second workflow, RNPxl, represents the first software solution to date for identification of peptide-RNA cross-links including automatic localization of the cross-links at amino acid resolution and localization scoring. It comes with a customized integrated cross-link fragment spectrum viewer for convenient manual inspection and validation of the results."],["dc.identifier.doi","10.1021/acs.jproteome.6b00407"],["dc.identifier.isi","000382713300044"],["dc.identifier.pmid","27476824"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39721"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1535-3907"],["dc.relation.issn","1535-3893"],["dc.title","LFQProfiler and RNPxl: Open-Source Tools for Label-Free Quantification and Protein-RNA Cross-Linking Integrated into Proteome Discoverer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Stützer, Alexandra"],["dc.contributor.author","Welp, Luisa M."],["dc.contributor.author","Raabe, Monika"],["dc.contributor.author","Sachsenberg, Timo"],["dc.contributor.author","Kappert, Christin"],["dc.contributor.author","Wulf, Alexander"],["dc.contributor.author","Lau, Andy M."],["dc.contributor.author","David, Stefan-Sebastian"],["dc.contributor.author","Chernev, Aleksandar"],["dc.contributor.author","Kramer, Katharina"],["dc.contributor.author","Politis, Argyris"],["dc.contributor.author","Kohlbacher, Oliver"],["dc.contributor.author","Fischle, Wolfgang"],["dc.contributor.author","Urlaub, Henning"],["dc.date.accessioned","2021-04-14T08:31:49Z"],["dc.date.available","2021-04-14T08:31:49Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1038/s41467-020-19047-7"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83722"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2041-1723"],["dc.title","Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]