Kaiser, Kristin

[["dc.bibliographiccitation.firstpage","2595"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Applied and Environmental Microbiology"],["dc.bibliographiccitation.lastpage","2607"],["dc.bibliographiccitation.volume","82"],["dc.contributor.author","Wüst, Pia K."],["dc.contributor.author","Nacke, Heiko"],["dc.contributor.author","Kaiser, Kristin"],["dc.contributor.author","Marhan, Sven"],["dc.contributor.author","Sikorski, Johannes"],["dc.contributor.author","Kandeler, Ellen"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.editor","Löffler, F. E."],["dc.date.accessioned","2020-12-10T18:36:50Z"],["dc.date.available","2020-12-10T18:36:50Z"],["dc.date.issued","2016"],["dc.description.abstract","Modern sequencing technologies allow high-resolution analyses of total and potentially active soil microbial communities based on their DNA and RNA, respectively. In the present study, quantitative PCR and 454 pyrosequencing were used to evaluate the effects of different extraction methods on the abundance and diversity of 16S rRNA genes and transcripts recovered from three different types of soils (leptosol, stagnosol, and gleysol). The quality and yield of nucleic acids varied considerably with respect to both the applied extraction method and the analyzed type of soil. The bacterial ribosome content (calculated as the ratio of 16S rRNA transcripts to 16S rRNA genes) can serve as an indicator of the potential activity of bacterial cells and differed by 2 orders of magnitude between nucleic acid extracts obtained by the various extraction methods. Depending on the extraction method, the relative abundances of dominant soil taxa, in particular Actinobacteria and Proteobacteria, varied by a factor of up to 10. Through this systematic approach, the present study allows guidelines to be deduced for the selection of the appropriate extraction protocol according to the specific soil properties, the nucleic acid of interest, and the target organisms."],["dc.identifier.doi","10.1128/AEM.00019-16"],["dc.identifier.eissn","1098-5336"],["dc.identifier.isi","000374640400003"],["dc.identifier.issn","0099-2240"],["dc.identifier.pmid","26896137"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76757"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1098-5336"],["dc.relation.issn","0099-2240"],["dc.title","Estimates of Soil Bacterial Ribosome Content and Diversity Are Significantly Affected by the Nucleic Acid Extraction Method Employed"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","33696"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Kaiser, Kristin"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.author","Korolkow, Vera"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Nacke, Heiko"],["dc.contributor.author","Schöning, Ingo"],["dc.contributor.author","Schrumpf, Marion"],["dc.contributor.author","Daniel, Rolf"],["dc.date.accessioned","2018-11-07T10:08:27Z"],["dc.date.available","2018-11-07T10:08:27Z"],["dc.date.issued","2016"],["dc.description.abstract","Soil bacteria provide a large range of ecosystem services such as nutrient cycling. Despite their important role in soil systems, compositional and functional responses of bacterial communities to different land use and management regimes are not fully understood. Here, we assessed soil bacterial communities in 150 forest and 150 grassland soils derived from three German regions by pyrotag sequencing of 16S rRNA genes. Land use type (forest and grassland) and soil edaphic properties strongly affected bacterial community structure and function, whereas management regime had a minor effect. In addition, a separation of soil bacterial communities by sampling region was encountered. Soil pH was the best predictor for bacterial community structure, diversity and function. The application of multinomial log-linear models revealed distinct responses of abundant bacterial groups towards pH. Predicted functional profiles revealed that differences in land use not only select for distinct bacterial populations but also for specific functional traits. The combination of 16S rRNA data and corresponding functional profiles provided comprehensive insights into compositional and functional adaptations to changing environmental conditions associated with differences in land use and management."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1038/srep33696"],["dc.identifier.isi","000383567400001"],["dc.identifier.pmid","27650273"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13778"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39463"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Driving forces of soil bacterial community structure, diversity, and function in temperate grasslands and forests"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2067"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Nacke, Heiko"],["dc.contributor.author","Goldmann, Kezia"],["dc.contributor.author","Schöning, Ingo"],["dc.contributor.author","Pfeiffer, Birgit"],["dc.contributor.author","Kaiser, Kristin"],["dc.contributor.author","Castillo-Villamizar, Genis A."],["dc.contributor.author","Schrumpf, Marion"],["dc.contributor.author","Buscot, François"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Wubet, Tesfaye"],["dc.date.accessioned","2019-07-09T11:43:04Z"],["dc.date.available","2019-07-09T11:43:04Z"],["dc.date.issued","2016"],["dc.description.abstract","The complex interactions between trees and soil microbes in forests as well as their inherent seasonal and spatial variations are poorly understood. In this study, we analyzed the effects of major European tree species (Fagus sylvatica L. and Picea abies (L.) Karst) on soil bacterial and fungal communities. Mineral soil samples were collected from different depths (0–10, 10–20 cm) and at different horizontal distances from beech or spruce trunks (0.5, 1.5, 2.5, 3.5 m) in early summer and autumn. We assessed the composition of soil bacterial and fungal communities based on 16S rRNA gene and ITS DNA sequences. Community composition of bacteria and fungi was most strongly affected by soil pH and tree species. Different ectomycorrhizal fungi (e.g., Tylospora) known to establish mutualistic associations with plant roots showed a tree species preference. Moreover, bacterial and fungal community composition showed spatial and seasonal shifts in soil surrounding beech and spruce. The relative abundance of saprotrophic fungi was higher at a depth of 0–10 vs. 10–20 cm depth. This was presumably a result of changes in nutrient availability, as litter input and organic carbon content decreased with soil depth. Overall bacterial community composition showed strong variations under spruce with increasing distance from the tree trunks, which might be attributed in part to higher fine root biomass near spruce trunks. Furthermore, overall bacterial community composition was strongly affected by season under deciduous trees."],["dc.identifier.doi","10.3389/fmicb.2016.02067"],["dc.identifier.pmid","28066384"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14106"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58817"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Fine Spatial Scale Variation of Soil Microbial Communities under European Beech and Norway Spruce"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]