Schönle, Andreas

[["dc.bibliographiccitation.firstpage","6266"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","6270"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Kunetsky, R."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:52Z"],["dc.date.available","2017-09-07T11:49:52Z"],["dc.date.issued","2007"],["dc.description.abstract","Exciting developments: Switching individual photochromic and fluorescent rhodamine amides enables 3D far-field optical microscopy with nanoscale resolution, excellent signal-to-noise ratio, and fast acquisition times. The rhodamine amides can be switched on using two photons, which enables 3D detailed imaging of thick and densely stained samples (such as 5-μm silica beads (see image) and living cells) to be constructed."],["dc.identifier.doi","10.1002/anie.200702167"],["dc.identifier.gro","3143552"],["dc.identifier.isi","000249114700006"],["dc.identifier.pmid","17640007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1078"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1433-7851"],["dc.title","Photochromic rhodamines provide nanoscopy with optical sectioning"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","L67"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","L69"],["dc.bibliographiccitation.volume","92"],["dc.contributor.author","Donnert, Gerald"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:49Z"],["dc.date.available","2017-09-07T11:49:49Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The stimulated emission depletion beams deliver a lateral resolution of < 30 nm and 65 nm for the green and the red color channel, respectively. The similar to 5 nm alignment accuracy of the two images establishes the precision with which differently labeled proteins are correlated in space. Colocalized nanoscopy is demonstrated with endosomal protein patterns and by resolving nanoclusters of a mitochondrial outer membrane protein, Tom20, in relation with the F(1)F(0)ATP synthase. The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells."],["dc.identifier.doi","10.1529/biophysj.107.104497"],["dc.identifier.gro","3143514"],["dc.identifier.isi","000245164000003"],["dc.identifier.pmid","17307826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1037"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Two-color far-field fluorescence nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3285"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","3290"],["dc.bibliographiccitation.volume","93"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","von Middendorff, Claas"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Wenzel, Dirk"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:23Z"],["dc.date.available","2017-09-07T11:49:23Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm."],["dc.identifier.doi","10.1529/biophysj.107.112201"],["dc.identifier.gro","3143415"],["dc.identifier.isi","000250199300033"],["dc.identifier.pmid","17660318"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/927"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","21093"],["dc.bibliographiccitation.issue","25"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","21104"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","von Middendorff, Claas"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Bossi, Mariano"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Egner, Alexander"],["dc.date.accessioned","2017-09-07T11:48:07Z"],["dc.date.available","2017-09-07T11:48:07Z"],["dc.date.issued","2008"],["dc.description.abstract","We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Subpopulations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells. (C) 2008 Optical Society of America"],["dc.identifier.doi","10.1364/OE.16.021093"],["dc.identifier.gro","3143194"],["dc.identifier.isi","000261563100097"],["dc.identifier.pmid","19065250"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/682"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","Nanoscale separation of molecular species based on their rotational mobility"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1159"],["dc.bibliographiccitation.issue","7233"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","1162"],["dc.bibliographiccitation.volume","457"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Ringemann, Christian"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Schwarzmann, Günter"],["dc.contributor.author","Sandhoff, Konrad"],["dc.contributor.author","Polyakova, Svetlana"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","von Middendorff, Claas"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:47:33Z"],["dc.date.available","2017-09-07T11:47:33Z"],["dc.date.issued","2009"],["dc.description.abstract","Cholesterol-mediated lipid interactions are thought to have a functional role in many membrane-associated processes such as signalling events(1-5). Although several experiments indicate their existence, lipid nanodomains ('rafts') remain controversial owing to the lack of suitable detection techniques in living cells(4,6-9). The controversy is reflected in their putative size of 5-200 nm, spanning the range between the extent of a protein complex and the resolution limit of optical microscopy. Here we demonstrate the ability of stimulated emission depletion (STED) far-field fluorescence nanoscopy(10) to detect single diffusing (lipid) molecules in nanosized areas in the plasma membrane of living cells. Tuning of the probed area to spot sizes similar to 70-fold below the diffraction barrier reveals that unlike phosphoglycerolipids, sphingolipids and glycosylphosphatidylinositol-anchored proteins are transiently (similar to 10-20 ms) trapped in cholesterol-mediated molecular complexes dwelling within <20-nm diameter areas. The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells."],["dc.identifier.doi","10.1038/nature07596"],["dc.identifier.gro","3143149"],["dc.identifier.isi","000263680100047"],["dc.identifier.pmid","19098897"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/631"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0028-0836"],["dc.title","Direct observation of the nanoscale dynamics of membrane lipids in a living cell"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2463"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","2468"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Boyarskiy, Vadim P."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:15Z"],["dc.date.available","2017-09-07T11:48:15Z"],["dc.date.issued","2008"],["dc.description.abstract","By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques."],["dc.identifier.doi","10.1021/nl801471d"],["dc.identifier.gro","3143264"],["dc.identifier.isi","000258440700061"],["dc.identifier.pmid","18642961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/759"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1530-6984"],["dc.title","Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","161"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Applied Physics B Lasers and Optics"],["dc.bibliographiccitation.lastpage","165"],["dc.bibliographiccitation.volume","88"],["dc.contributor.author","Bock, H."],["dc.contributor.author","Geisler, C."],["dc.contributor.author","Wurm, C. A."],["dc.contributor.author","von Middendorff, C."],["dc.contributor.author","Jakobs, S."],["dc.contributor.author","Schönle, A."],["dc.contributor.author","Egner, A."],["dc.contributor.author","Hell, S. W."],["dc.contributor.author","Eggeling, C."],["dc.date.accessioned","2017-09-07T11:49:27Z"],["dc.date.available","2017-09-07T11:49:27Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate two-color far-field fluorescence microscopy with nanoscale spatial resolution based on the photoswitching of individual fluorescent markers. By enabling, recording, and disabling the emission of the reversibly switchable fluorescent protein rsFastLime and of the organic fluorophore cyanine5, we recorded two-color nanoscale images inside whole cells. The position of individual emitters was determined with a typical accuracy of 20 nm, which largely constitutes the lateral resolution of the system. Photoswitching in two-color colocalization experiments represents a major step towards the application of far-field fluorescence nanoscopy to the study of (biological) samples on the macromolecular level."],["dc.identifier.doi","10.1007/s00340-007-2729-0"],["dc.identifier.gro","3143472"],["dc.identifier.isi","000248054900001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/989"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0946-2171"],["dc.title","Two-color far-field fluorescence nanoscopy based on photoswitchable emitters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","528"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Microscopy Research and Technique"],["dc.bibliographiccitation.lastpage","536"],["dc.bibliographiccitation.volume","77"],["dc.bibliographiccitation.volumetitle","Fluorescence Microscopy in the Spotlight"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","von Middendorff, Claas"],["dc.contributor.author","Ringemann, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.date.accessioned","2017-09-07T11:46:11Z"],["dc.date.available","2017-09-07T11:46:11Z"],["dc.date.issued","2014"],["dc.description.abstract","A marker's dark triplet state is of great importance in fluorescence microscopy: It serves as a means to switch off fluorescent markers and is thus the enabling element for several super-resolution methods. On the other hand, intersystem-crossing to the electronic dark triplet state strongly reduces the fluorescence yield in conventional fluorescence microscopy. The ability to determine the kinetic parameters of transitions into the triplet state is thus of great importance and because fluorescence correlation spectroscopy (FCS) can be applied without disturbing the system under study, it is one of the preferred methods to do so. However, conventional FCS observations of triplet dynamics suffer from bias due to the spatially inhomogeneous irradiance profile of the excitation laser. Herein, we present a novel method to correct this bias and verify it by analyzing both Monte Carlo simulated and experimental data of the organic dye Rhodamine 110 in aqueous solution for both continuous-wave and pulsed excitation. Importantly, our approach can be readily generalized to most other FCS experiments that determine intensity dependent kinetic parameters."],["dc.identifier.doi","10.1002/jemt.22368"],["dc.identifier.gro","3142100"],["dc.identifier.isi","000338013200007"],["dc.identifier.pmid","24729575"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4544"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: BMBF [0312020A]; DFG [SFB755]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1097-0029"],["dc.relation.issn","1059-910X"],["dc.title","Monitoring Triplet State Dynamics With Fluorescence Correlation Spectroscopy: Bias and Correction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","321"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","326"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Riedel, Dietmar"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:47Z"],["dc.date.available","2017-09-07T11:48:47Z"],["dc.date.issued","2008"],["dc.description.abstract","During the lost decade for-field fluorescence microscopy methods have evolved that have resolution for below the wavelength of light. To outperform the limiting role of diffraction, all these methods, in one way or another, switch the ability of a molecule to emit fluorescence. Here we present a novel rhodamine amide that can be photoswitched from a nonfluorescent to a fluorescent state by absorption of one or two photons from a continuous-wave loser beam. This bright marker enables strict control of on/off switching and provides single-molecule localization precision down to 15 nm in the focal plane. Two-photon induced nonlinear photoswitching of this marker with continuous-wave illumination offers optical sectioning with simple loser equipment. Future synthesis of similar compounds holds great promise for cost-effective fluorescence nanoscopy with noninvasive optical sectioning."],["dc.identifier.doi","10.1002/cphc.200700655"],["dc.identifier.gro","3143351"],["dc.identifier.isi","000253177700017"],["dc.identifier.pmid","18200483"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/855"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1439-4235"],["dc.title","Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","5412"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Honigmann, Alf"],["dc.contributor.author","Mueller, Veronika"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Sezgin, Erdinc"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.date.accessioned","2017-09-07T11:45:25Z"],["dc.date.available","2017-09-07T11:45:25Z"],["dc.date.issued","2014"],["dc.description.abstract","The interaction of lipids and proteins plays an important role in plasma membrane bioactivity, and much can be learned from their diffusion characteristics. Here we present the combination of super-resolution STED microscopy with scanning fluorescence correlation spectroscopy (scanning STED-FCS, sSTED-FCS) to characterize the spatial and temporal heterogeneity of lipid interactions. sSTED-FCS reveals transient molecular interaction hotspots for a fluorescent sphingolipid analogue. The interaction sites are smaller than 80nm in diameter and lipids are transiently trapped for several milliseconds in these areas. In comparison, newly developed fluorescent phospholipid and cholesterol analogues with improved phase-partitioning properties show more homogenous diffusion, independent of the preference for liquid-ordered or disordered membrane environments. Our results do not support the presence of nanodomains based on lipid-phase separation in the basal membrane of our cultured nonstimulated cells, and show that alternative interactions are responsible for the strong local trapping of our sphingolipid analogue."],["dc.identifier.doi","10.1038/ncomms6412"],["dc.identifier.gro","3142023"],["dc.identifier.isi","000345624800033"],["dc.identifier.pmid","25410140"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3690"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2041-1723"],["dc.title","Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]