Spalthoff, Christian

[["dc.bibliographiccitation.artnumber","17085"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Karak, Somdatta"],["dc.contributor.author","Jacobs, Julie S."],["dc.contributor.author","Kittelmann, Maike"],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Katana, Radoslaw"],["dc.contributor.author","Sivan-Loukianova, Elena"],["dc.contributor.author","Schon, Michael A."],["dc.contributor.author","Kernan, Maurice J."],["dc.contributor.author","Eberl, Daniel F."],["dc.contributor.author","Göpfert, Martin C."],["dc.date.accessioned","2018-11-07T09:48:47Z"],["dc.date.available","2018-11-07T09:48:47Z"],["dc.date.issued","2015"],["dc.description.abstract","Much like vertebrate hair cells, the chordotonal sensory neurons that mediate hearing in Drosophila are motile and amplify the mechanical input of the ear. Because the neurons bear mechanosensory primary cilia whose microtubule axonemes display dynein arms, we hypothesized that their motility is powered by dyneins. Here, we describe two axonemal dynein proteins that are required for Drosophila auditory neuron function, localize to their primary cilia, and differently contribute to mechanical amplification in hearing. Promoter fusions revealed that the two axonemal dynein genes Dmdnah3 (=CG17150) and Dmdnai2 (=CG6053) are expressed in chordotonal neurons, including the auditory ones in the fly's ear. Null alleles of both dyneins equally abolished electrical auditory neuron responses, yet whereas mutations in Dmdnah3 facilitated mechanical amplification, amplification was abolished by mutations in Dmdnai2. Epistasis analysis revealed that Dmdnah3 acts downstream of Nan-Iav channels in controlling the amplificatory gain. Dmdnai2, in addition to being required for amplification, was essential for outer dynein arms in auditory neuron cilia. This establishes diverse roles of axonemal dyneins in Drosophila auditory neuron function and links auditory neuron motility to primary cilia and axonemal dyneins. Mutant defects in sperm competition suggest that both dyneins also function in sperm motility."],["dc.description.sponsorship","Open-Access Publikationsfonds 2015"],["dc.identifier.doi","10.1038/srep17085"],["dc.identifier.isi","000365389300001"],["dc.identifier.pmid","26608786"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12601"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35376"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Diverse Roles of Axonemal Dyneins in Drosophila Auditory Neuron Function and Mechanical Amplification in Hearing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Abstracts of Papers of the American Chemical Society"],["dc.bibliographiccitation.volume","248"],["dc.contributor.author","Salgado, Vincent L."],["dc.contributor.author","Nesterov, Alexandre"],["dc.contributor.author","Kandasamy, Ramani A."],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Göpfert, Martin C."],["dc.date.accessioned","2018-11-07T09:36:41Z"],["dc.date.available","2018-11-07T09:36:41Z"],["dc.date.issued","2014"],["dc.identifier.isi","000349165100596"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32673"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.publisher.place","Washington"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","0065-7727"],["dc.title","Action of insecticides on chordotonal organs"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","665"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","671"],["dc.bibliographiccitation.volume","86"],["dc.contributor.author","Nesterov, Alexandre"],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Kandasamy, Ramani A."],["dc.contributor.author","Katana, Radoslav"],["dc.contributor.author","Rankl, Nancy B."],["dc.contributor.author","Andres, Marta"],["dc.contributor.author","Jaehde, Philipp"],["dc.contributor.author","Dorsch, John A."],["dc.contributor.author","Stam, Lynn F."],["dc.contributor.author","Braun, Franz-Josef"],["dc.contributor.author","Warren, Ben"],["dc.contributor.author","Salgado, Vincent L."],["dc.contributor.author","Göpfert, Martin C."],["dc.date.accessioned","2018-11-07T09:57:21Z"],["dc.date.available","2018-11-07T09:57:21Z"],["dc.date.issued","2015"],["dc.description.abstract","Defining the molecular targets of insecticides is crucial for assessing their selectivity and potential impact on environment and health. Two commercial insecticides are now shown to target a transient receptor potential (TRP) ion channel complex that is unique to insect stretch receptor cells. Pymetrozine and pyrifluquinazon disturbed Drosophila coordination and hearing by acting on chordotonal stretch receptor neurons. This action required the two TRPs Nanchung (Nan) and Inactive (Iav), which co-occur exclusively within these cells. Nan and Iav together sufficed to confer cellular insecticide responses in vivo and in vitro, and the two insecticides were identified as specific agonists of Nan-Iav complexes that, by promoting cellular calcium influx, silence the stretch receptor cells. This establishes TRPs as insecticide targets and defines specific agonists of insect TRPs. It also shows that TRPs can render insecticides cell-type selective and puts forward TRP targets to reduce side effects on non-target species."],["dc.identifier.doi","10.1016/j.neuron.2015.04.001"],["dc.identifier.isi","000354069800009"],["dc.identifier.pmid","25950634"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37137"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1097-4199"],["dc.relation.issn","0896-6273"],["dc.title","TRP Channels in Insect Stretch Receptors as Insecticide Targets"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","72"],["dc.bibliographiccitation.journal","Frontiers in Neural Circuits"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Gerdes, Ralf"],["dc.contributor.author","Kurtz, Rafael"],["dc.date.accessioned","2019-07-09T11:55:01Z"],["dc.date.available","2019-07-09T11:55:01Z"],["dc.date.issued","2012-10-09"],["dc.description.abstract","In insects, the first extraction of motion and direction clues from local brightness modulations is thought to take place in the medulla. However, whether and how these computations are represented in the medulla stills remain widely unknown, because electrical recording of the neurons in the medulla is difficult. As an effort to overcome this difficulty, we employed local electroporation in vivo in the medulla of the blowfly (Calliphora vicina) to stain small ensembles of neurons with a calcium-sensitive dye. We studied the responses of these neuronal ensembles to spatial and temporal brightness modulations and found selectivity for grating orientation. In contrast, the responses to the two opposite directions of motion of a grating with the same orientation were similar in magnitude, indicating that strong directional selectivity is either not present in the types of neurons covered by our data set, or that direction-selective signals are too closely spaced to be distinguished by our calcium imaging. The calcium responses also showed a bell-shaped dependency on the temporal frequency of drifting gratings, with an optimum higher than that observed in one of the subsequent processing stages, i.e., the lobula plate. Medulla responses were elicited by on- as well as off-stimuli with some spatial heterogeneity in the sensitivity for “on” and “off”, and in the polarity of the responses. Medulla neurons thus show similarities to some established principles of motion and edge detection in the vertebrate visual system."],["dc.identifier.doi","10.3389/fncir.2012.00072"],["dc.identifier.fs","593642"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9978"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60780"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1662-5110"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Neuronal representation of visual motion and orientation in the fly medulla"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","8"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Göpfert, Martin C."],["dc.date.accessioned","2018-11-07T10:11:43Z"],["dc.date.available","2018-11-07T10:11:43Z"],["dc.date.issued","2016"],["dc.description.abstract","Transmembrane channel-like (TMC) proteins have been implicated in hair cell mechanotransduction, Drosophila proprioception, and sodium sensing in the nematode C. elegans. In this issue of Neuron, Wang et al. (2016) report that C. elegans TMC-1 mediates nociceptor responses to high pH, not sodium, allowing the nematode to avoid strongly alkaline environments in which most animals cannot survive."],["dc.identifier.doi","10.1016/j.neuron.2016.06.026"],["dc.identifier.isi","000382394300003"],["dc.identifier.pmid","27387645"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40104"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1097-4199"],["dc.relation.issn","0896-6273"],["dc.title","Sensing pH with TMCs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2796"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2804"],["dc.bibliographiccitation.volume","113"],["dc.contributor.author","Prahlad, Achintya"],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Kong, Deqing"],["dc.contributor.author","Großhans, Jörg"],["dc.contributor.author","Göpfert, Martin C."],["dc.contributor.author","Schmidt, Christoph F."],["dc.date.accessioned","2020-12-10T14:22:44Z"],["dc.date.available","2020-12-10T14:22:44Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1016/j.bpj.2017.08.061"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71712"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Mechanical Properties of a Drosophila Larval Chordotonal Organ"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","ps.7101"],["dc.bibliographiccitation.journal","Pest Management Science"],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Salgado, Vincent L."],["dc.contributor.author","Theis, Mario"],["dc.contributor.author","Geurten, Bart R. H."],["dc.contributor.author","Göpfert, Martin C."],["dc.date.accessioned","2022-09-01T09:50:37Z"],["dc.date.available","2022-09-01T09:50:37Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1002/ps.7101"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113756"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","1526-4998"],["dc.relation.issn","1526-498X"],["dc.title","Flonicamid metabolite 4‐trifluoromethylnicotinamide is a chordotonal organ modulator insecticide\n †"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Prahlad, A."],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Warren, Ben"],["dc.contributor.author","Kong, Deqing"],["dc.contributor.author","Grosshans, Joerg"],["dc.contributor.author","Göpfert, Martin C."],["dc.contributor.author","Schmidt, C. F."],["dc.date.accessioned","2018-11-07T10:19:35Z"],["dc.date.available","2018-11-07T10:19:35Z"],["dc.date.issued","2016"],["dc.identifier.isi","000396046900963"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41692"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.publisher.place","Bethesda"],["dc.relation.conference","Annual Meeting of the American-Society-for-Cell-Biology (ASCB)"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","1939-4586"],["dc.relation.issn","1059-1524"],["dc.title","Mechanical properties of the lch5 organ of Drosophila."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","R950"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Current Biology"],["dc.bibliographiccitation.lastpage","R952"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Geurten, Bart R. H."],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Göpfert, Martin C."],["dc.date.accessioned","2018-11-07T09:17:41Z"],["dc.date.available","2018-11-07T09:17:41Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1016/j.cub.2013.09.044"],["dc.identifier.isi","000326993600006"],["dc.identifier.pmid","24200319"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28225"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1879-0445"],["dc.relation.issn","0960-9822"],["dc.title","Insect Hearing: Active Amplification in Tympanal Ears"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2028"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Current Biology"],["dc.bibliographiccitation.lastpage","2036"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Andres, Marta"],["dc.contributor.author","Seifert, Marvin"],["dc.contributor.author","Spalthoff, Christian"],["dc.contributor.author","Warren, Ben"],["dc.contributor.author","Weiss, Lukas"],["dc.contributor.author","Giraldo, Diego"],["dc.contributor.author","Winkler, Margret"],["dc.contributor.author","Pauls, Stephanie"],["dc.contributor.author","Göpfert, Martin C."],["dc.date.accessioned","2018-11-07T10:10:16Z"],["dc.date.available","2018-11-07T10:10:16Z"],["dc.date.issued","2016"],["dc.description.abstract","The performance of vertebrate ears is controlled by auditory efferents that originate in the brain and innervate the ear, synapsing onto hair cell somata and auditory afferent fibers [1-3]. Efferent activity can provide protection from noise and facilitate the detection and discrimination of sound by modulating mechanical amplification by hair cells and transmitter release as well as auditory afferent action potential firing [1-3]. Insect auditory organs are thought to lack efferent control [4-7], but when we inspected mosquito ears, we obtained evidence for its existence. Antibodies against synaptic proteins recognized rows of bouton-like puncta running along the dendrites and axons of mosquito auditory sensory neurons. Electron microscopy identified synaptic and non-synaptic sites of vesicle release, and some of the innervating fibers co-labeled with somata in the CNS. Octopamine, GABA, and serotonin were identified as efferent neurotransmitters or neuromodulators that affect auditory frequency tuning, mechanical amplification, and sound-evoked potentials. Mosquito brains thus modulate mosquito ears, extending the use of auditory efferent systems from vertebrates to invertebrates and adding new levels of complexity to mosquito sound detection and communication."],["dc.identifier.doi","10.1016/j.cub.2016.05.077"],["dc.identifier.isi","000381241100026"],["dc.identifier.pmid","27476597"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39818"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1879-0445"],["dc.relation.issn","0960-9822"],["dc.title","Auditory Efferent System Modulates Mosquito Hearing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]