Hauke, Lara

[["dc.bibliographiccitation.firstpage","1595"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Huber, René"],["dc.contributor.author","Attili/Abedalkhader, Rozan"],["dc.contributor.author","Küper, Daniela"],["dc.contributor.author","Hauke, Lara"],["dc.contributor.author","Lüns, Bernadette"],["dc.contributor.author","Brand, Korbinian"],["dc.contributor.author","Weissenborn, Karin"],["dc.contributor.author","Lichtinghagen, Ralf"],["dc.date.accessioned","2022-06-08T07:57:35Z"],["dc.date.available","2022-06-08T07:57:35Z"],["dc.date.issued","2019"],["dc.description.abstract","Blood sampling with different anticoagulants alters matrix metalloproteinase (MMP-) 9 expression, thus influencing its concentration and diagnostic validity. Here, we aimed to evaluate the effects of different anticoagulants on MMP-9 regulation. MMP-9 expression was assessed in response to ethylenediaminetetraacetic acid, citrate, and high-/low-molecular-weight heparin (HMWH, LMWH) in co-culture experiments using THP-1, Jurkat, and HT cells (representing monocytes, T, and B cells). Triple and double cell line co-culture experiments revealed that HMWH treatment of THP-1 and Jurkat led to a significant MMP-9 induction, whereas other anticoagulants and cell type combinations had no effect. Supernatant of HMWH-treated Jurkat cells also induced MMP-9 in THP-1 suggesting monocytes as MMP-9 producers. HMWH-induced cytokine/chemokine secretion was assessed in co-culture supernatant, and the influence of cytokines/chemokines on MMP-9 production was analyzed. These experiments revealed that Jurkat-derived IL-16 and soluble intercellular adhesion molecule (sICAM-) 1 are able to induce MMP-9 and IL-8 production by THP-1. As a consequence, the increased MMP-9 expression found in HMWH blood samples may be influenced by HMWH-dependent secretion of IL-16 and sICAM-1 by T cells resulting in an increased production of MMP-9 and IL-8 by monocytes. IL-8, in turn, may support MMP-9 and its own expression in a positive autocrine feedback loop."],["dc.identifier.doi","10.3390/ijms20071595"],["dc.identifier.pii","ijms20071595"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/110141"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-575"],["dc.relation.eissn","1422-0067"],["dc.title","Cellular and Molecular Effects of High-Molecular-Weight Heparin on Matrix Metalloproteinase 9 Expression"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]