Pieler, Tomas

[["dc.bibliographiccitation.firstpage","352"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Genomics"],["dc.bibliographiccitation.lastpage","364"],["dc.bibliographiccitation.volume","86"],["dc.contributor.author","Cimica, Velasco"],["dc.contributor.author","Batusic, D."],["dc.contributor.author","Chen, Y. L."],["dc.contributor.author","Hollemann, T."],["dc.contributor.author","Pieler, T."],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T10:56:09Z"],["dc.date.available","2018-11-07T10:56:09Z"],["dc.date.issued","2005"],["dc.description.abstract","We have performed serial analysis of gene expression of the regenerating liver. In the rat model of partial hepatectomy and 2-acetamidofluorene treatment liver regeneration recruits hepatic stem cells referred to as oval cells. We analyzed a total of 153,057 tags in livers from normal control (52,343 tags), from sham 2-acetamidofluorene-treated control (50,502 tags), and from the early stage of oval cell proliferation (50,212 tags). Comparative analysis of the three transcriptomes identified 27 up-regulated and 18 down-regulated genes. Real-time PCR analysis confirmed I I temporally regulated genes that correlate with oval cell development. Interestingly, we found by Western blot protein analysis of regenerating livers that the cell cycle gene Cdc42 was induced concomitant with the proliferation marker cyclin D I and the oval cell marker alpha-fetoprotein. Our studies provide new insights into the molecular mechanism of liver regeneration through oval cells. (c) 2005 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.ygeno.2005.05.001"],["dc.identifier.isi","000231350300011"],["dc.identifier.pmid","15993033"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49946"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","0888-7543"],["dc.title","Transcriptome analysis of rat liver regeneration in a model of oval hepatic stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","296"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","315"],["dc.bibliographiccitation.volume","277"],["dc.contributor.author","Cornesse, Y."],["dc.contributor.author","Pieler, T."],["dc.contributor.author","Hollemann, T."],["dc.date.accessioned","2018-11-07T08:32:22Z"],["dc.date.available","2018-11-07T08:32:22Z"],["dc.date.issued","2005"],["dc.description.abstract","The integration of multiple signaling pathways is a key issue in several aspects of embryonic development. In this context, extracellular inhibitors of secreted growth factors play an important role, which is to antagonize specifically the activity of the corresponding signaling previously described as a Hedgehog-specific molecule. We provide evidence that the Hedgehog interacting protein (Hip) from Xenopus. antagonist in the mouse, interferes with Wnt-8 and eFgf/Faf-8 signaling pathways as well. To address the function of Hip during early embryonic development, we performed gain- and loss-of-function studies in the frog. Overexpression of Xhip or mHip1 resulted in a dramatic increase of retinal structures and larger olfactory placodes primarily at the expense of other brain tissues. Furthermore, loss of Xhip function resulted in a suppression of olfactory and lens placode formation. Therefore. the localized expression of Xhip may counteract certain overlapping signaling activities, which inhibit the induction of distinct sensory placodes. (C) 2004 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.ydbio.2004.09.016"],["dc.identifier.isi","000226225700003"],["dc.identifier.pmid","15617676"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17325"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","0012-1606"],["dc.title","Olfactory and lens placode formation is controlled by the hedgehog-interacting protein (Xhip) in Xenopus"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","245"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Mechanisms of Development"],["dc.bibliographiccitation.lastpage","248"],["dc.bibliographiccitation.volume","95"],["dc.contributor.author","Wischnewski, J."],["dc.contributor.author","Solter, M."],["dc.contributor.author","Chen, Y. L."],["dc.contributor.author","Hollemann, T."],["dc.contributor.author","Pieler, T."],["dc.date.accessioned","2018-11-07T10:42:15Z"],["dc.date.available","2018-11-07T10:42:15Z"],["dc.date.issued","2000"],["dc.description.abstract","Karyopherin-beta 3 is a nuclear transport receptor that appears to be involved in nuclear import of ribosomal proteins. Here we report on sequence and expression of karyopherin-beta 3 in Xenopus. The differential distribution of karyopherin-beta 3 mRNA during Xenopus embryogenesis is similar to that of several other protein import factors and of ribosomal proteins. These genes thus define a novel synexpression group in the context of ribosome biogenesis. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S0925-4773(00)00337-3"],["dc.identifier.isi","000088607200026"],["dc.identifier.pmid","10906471"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46748"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0925-4773"],["dc.title","Structure and expression of Xenopus karyopherin-beta 3: definition of a novel synexpression group related to ribosome biogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","277"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Mechanisms of Development"],["dc.bibliographiccitation.lastpage","288"],["dc.bibliographiccitation.volume","120"],["dc.contributor.author","Chen, Y."],["dc.contributor.author","Juergens, K."],["dc.contributor.author","Hollemann, T."],["dc.contributor.author","Claussen, M."],["dc.contributor.author","Ramadori, Giuliano"],["dc.contributor.author","Pieler, T."],["dc.date.accessioned","2018-11-07T10:40:41Z"],["dc.date.available","2018-11-07T10:40:41Z"],["dc.date.issued","2003"],["dc.description.abstract","Early regulatory events in respect to the embryonic development of the vertebrate liver are only poorly defined. A better understanding of the gene network that mediates the formation of hepatocytes from pluripotent embryonic precursor cells may help to establish in vitro protocols for hepatocyte differentiation. Here, we describe our first attempts to make use of early embryonic explants from the amphibian Xenopus laevis in order to address these questions. We have identified several novel embryonic liver and intestine marker genes in a random expression pattern screen with cDNA libraries derived from the embryonic liver anlage and from the adult liver of Xenopus laevis. Based on their embryonic expression characteristics, these genes, together with the previously known ones, can be categorized into four different groups: the liver specific group (LS), the liver and intestine group A (LIA), the liver and intestine group B (LIB), and the intestine specific group (IS). Dissociation of endodermal explants isolated from early neurula stage embryos reveals that all genes in the LIB and IS groups are expressed in a cell-autonomous manner. In contrast, expression of genes in the LS and LIA groups requires cell-cell interactions. The regular temporal expression profile of genes in all four groups is mimicked in ectodermal explants from early embryos, reprogrammed by co-injection of VegT and P-catenin mRNAs. FGF signaling is found to be required for the induction of liver specific marker (LS group) gene expression in the same system. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S0925-4773(02)00460-4"],["dc.identifier.isi","000181258700002"],["dc.identifier.pmid","12591597"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46359"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0925-4773"],["dc.title","Cell-autonomous and signal-dependent expression of liver and intestine marker genes in pluripotent precursor cell's from Xenopus embryos"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","53"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Mechanisms of Development"],["dc.bibliographiccitation.lastpage","63"],["dc.bibliographiccitation.volume","90"],["dc.contributor.author","Chen, Y. L."],["dc.contributor.author","Hollemann, T."],["dc.contributor.author","Pieler, T."],["dc.contributor.author","Grunz, H."],["dc.date.accessioned","2018-11-07T11:11:12Z"],["dc.date.available","2018-11-07T11:11:12Z"],["dc.date.issued","2000"],["dc.description.abstract","Early observations on the morphology of total exogastrulae from urodeles (Axolotl) had provided evidence for essential vertical signalling mechanisms in the process of neural induction. Conversely, more recent studies with anurans (Xenopus laevis) making use of molecular markers for neural-specific gene expression appear to support the idea of planar signalling as providing sufficient information for neural differentiation along the anterior-posterior axis. In an attempt to resolve this apparent contradiction, we report on the comparative analysis of morphology and gene expression characteristics with explants prepared from both urodeles (Triturus alpestris) and anurans (Xenopus laevis). For this purpose, we have made use of a refined experimental protocol for the preparation of exogastrulae that is intended to combine the advantages of the Holtfreter type exogastrula and the Keller sandwich techniques, and which we refer to as pseudoexogastrula explants. Analysis of histology and expression of several neural and ectodermal marker genes in such explants suggests that neural differentiation is induced in both species, but only within the intermediate zone between ectoderm and endomesoderm. Therefore, experiments with Xenopus and Triturus explants described in this communication argue against planar signalling events as being sufficient to generate a specific anterior/posterior neural pattern. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S0925-4773(99)00229-4"],["dc.identifier.isi","000084306300005"],["dc.identifier.pmid","10585562"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53375"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0925-4773"],["dc.title","Planar signalling is not sufficient to generate a specific anterior/posterior neural pattern in pseudoexogastrula explants from Xenopus and Triturus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1135"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","EUROPEAN JOURNAL OF BIOCHEMISTRY"],["dc.bibliographiccitation.lastpage","1144"],["dc.bibliographiccitation.volume","271"],["dc.contributor.author","Durr, U."],["dc.contributor.author","Henningfeld, Kristine A."],["dc.contributor.author","Hollemann, T."],["dc.contributor.author","Knochel, W."],["dc.contributor.author","Pieler, T."],["dc.date.accessioned","2018-11-07T10:50:34Z"],["dc.date.available","2018-11-07T10:50:34Z"],["dc.date.issued","2004"],["dc.description.abstract","The HIVEP gene family encodes for very large sequence-specific DNA binding proteins containing multiple zinc fingers. Three mammalian paralogous genes have been identified, HIVEP1, -2 and -3, as well as the closely related Drosophila gene, Schnurri. These genes have been found to directly participate in the transcriptional regulation of a variety of genes. Mammalian HIVEP members have been implicated in signaling by TNF-alpha and in the positive selection of thymocytes, while Schnurri has been shown to be an essential component of the TGF-beta signaling pathway. In this study, we describe the isolation of Xenopus HIVEP1, as well as partial cDNAs of HIVEP2 and -3. Analysis of the temporal and spatial expression of the XHIVEP transcripts during early embryogenesis revealed ubiquitous expression of the transcripts. Assays using Xenopus oocytes mapped XHIVEP1 domains that are responsible for nuclear export and import activity. The DNA binding specificity of XHIVEP was characterized using a PCR-mediated selection and gel mobility shift assays."],["dc.identifier.doi","10.1111/j.1432-1033.2004.04017.x"],["dc.identifier.isi","000220006500008"],["dc.identifier.pmid","15009192"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48688"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing Ltd"],["dc.relation.issn","0014-2956"],["dc.title","Isolation and characterization of the Xenopus HIVEP gene family"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","325"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","338"],["dc.bibliographiccitation.volume","260"],["dc.contributor.author","Koebernick, K."],["dc.contributor.author","Hollemann, T."],["dc.contributor.author","Pieler, T."],["dc.date.accessioned","2018-11-07T10:36:57Z"],["dc.date.available","2018-11-07T10:36:57Z"],["dc.date.issued","2003"],["dc.description.abstract","Vertebrate inner ear development is initiated by the specification of the otic placode, an ectodermal structure induced by signals from neighboring tissue. Although several signaling molecules have been identified as candidate otic inducers, many details of the process of inner ear induction remain elusive. Here, we report that otic induction is responsive to the level of Hedgehog (Hh) signaling activity in Xenopus, making use of both gain- and loss-of-function approaches. Ectopic activation of Hedgehog signaling resulted in the development of ectopic vesicular structures expressing the otic marker genes XPax-2, Xdll-3, and Xwnt-3A, thus revealing otic identity. Induction of ectopic otic vesicles was also achieved by misexpression of two different inhibitors of Hh signaling: the putative Hh antagonist mHIP and XPtc lDeltaLoop2, a dominant-negative form of the Hh receptor Patched. In addition, misexpression of XPtc I DeltaLoop2 as well as treatment of Xenopus embryos with the specific Hh signaling antagonist cyclopamine resulted in the formation of enlarged otic vesicles. In summary, our observations suggest that a defined level of Hh signaling provides a restrictive environment for otic fate in Xenopus embryos. (C) 2003 Elsevier Science (USA). All rights reserved."],["dc.identifier.doi","10.1016/S0012-1606(03)00242-2"],["dc.identifier.isi","000184946000004"],["dc.identifier.pmid","12921735"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45447"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","0012-1606"],["dc.title","A restrictive role for Hedgehog signalling during otic specification in Xenopus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","255"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Mechanisms of Development"],["dc.bibliographiccitation.lastpage","257"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Pommereit, D."],["dc.contributor.author","Pieler, T."],["dc.contributor.author","Hollemann, T."],["dc.date.accessioned","2018-11-07T09:13:38Z"],["dc.date.available","2018-11-07T09:13:38Z"],["dc.date.issued","2001"],["dc.description.abstract","Xpitx3 is the Xenopus homologue of the mouse Pitx3 gene and belongs to the family of RIEG/PITX homeobox genes, Here, we report on the embryonic expression of Xpitx3. It is transcribed in the presumptive pituitary already at the open neural tube stage. During further development Xpitx3 is strongly transcribed in the pituitary Anlage, the lens placodes and head mesenchyme, respectively. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S0925-4773(01)00305-7"],["dc.identifier.isi","168571500029"],["dc.identifier.pmid","11287205"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27225"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0925-4773"],["dc.title","Xpitx3: a member of the Rieg/Pitx gene family expressed during pituitary and lens formation in Xenopus laevis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","579"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Development Genes and Evolution"],["dc.bibliographiccitation.lastpage","581"],["dc.bibliographiccitation.volume","210"],["dc.contributor.author","Hollemann, T."],["dc.contributor.author","Pieler, T."],["dc.date.accessioned","2018-11-07T11:15:53Z"],["dc.date.available","2018-11-07T11:15:53Z"],["dc.date.issued","2000"],["dc.description.abstract","Nkx-2.1 is a member of the vertebrate Nkx family of homeobox genes; it was originally identified as a tissue-specific regulator of thyroglobulin and thyroperoxidase gene transcription. Here we report on the embryonic expression of Xnkx-2.1, which is expressed in the presumptive forebrain from early neurulation onwards. In tadpole stage embryos Xnkx-2.1 transcripts are primarily detected in ventral forebrain, lung buds and thyroid anlage. Therefore, Xnkx-2.1 may be part of the genetic network that controls the early development of these organs."],["dc.identifier.doi","10.1007/s004270000098"],["dc.identifier.isi","165416400008"],["dc.identifier.pmid","11180810"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54467"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0949-944X"],["dc.title","Xnkx-2.1: a homeobox gene expressed during early forebrain, lung and thyroid development in Xenopus laevis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","245"],["dc.bibliographiccitation.issue","3-4"],["dc.bibliographiccitation.journal","Histochemistry and Cell Biology"],["dc.bibliographiccitation.lastpage","260"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Batusic, Danko S."],["dc.contributor.author","Cimica, Velasco"],["dc.contributor.author","Chen, Y. L."],["dc.contributor.author","Tron, Kyrylo"],["dc.contributor.author","Hollemann, T."],["dc.contributor.author","Pieler, T."],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T10:56:11Z"],["dc.date.available","2018-11-07T10:56:11Z"],["dc.date.issued","2005"],["dc.description.abstract","Under certain conditions liver regeneration can be accomplished by hepatic progenitor cells (\"oval cells\"). So far, only few factors have been identified to be uniquely regulated by the \"oval cell\" compartment. Using macroarray analysis in a rat model of oval cell proliferation (treatment with 2-acetylaminofluorene and partial hepatectomy, AAF + PH), we identified 12 differentially expressed genes compared to appropriate control models (AAF treatment and sham operation or AAF treatment alone). Further analysis in models of normal liver regeneration (ordinary PH) and acute phase response (turpentine oil-treated rats) revealed that three out of 12 genes (thymidine kinase 1, Jun-D and ADP-ribosylation factor 4) were not affected by the hepatic acute phase reaction but similarly overexpressed in both \"oval cell\" -dependant and normal liver regeneration. We characterized Jun-D and ADP-ribosylation factors as novel factors upregulated in oval cells and in nonparenchymal liver cells of normally regenerating livers. However, two out of 12 differentially expressed genes were specifically expressed in oval cells: ras-related protein Rab-3b and Ear-2. On protein level, Rab-3b was increased in total liver homogenates and demonstrated only in clusters of oval cells. We postulate that Ear-2 and Rab-3b may represent novel regulatory factors specifically activated in \"oval cells\"."],["dc.identifier.doi","10.1007/s00418-005-0021-0"],["dc.identifier.isi","000233012600007"],["dc.identifier.pmid","16044259"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49955"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0948-6143"],["dc.title","Identification of genes specific to rat 2-acetylaminofluorene/partial 'oval cells\" in the hepatectomy model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]