Pieler, Tomas

[["dc.bibliographiccitation.firstpage","4097"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","Development"],["dc.bibliographiccitation.lastpage","4108"],["dc.bibliographiccitation.volume","133"],["dc.contributor.author","Soelter, Marion"],["dc.contributor.author","Locker, Morgane"],["dc.contributor.author","Boy, Sebastien"],["dc.contributor.author","Taelman, Vincent"],["dc.contributor.author","Bellefroid, Eric J."],["dc.contributor.author","Perron, Muriel"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2018-11-07T09:06:26Z"],["dc.date.available","2018-11-07T09:06:26Z"],["dc.date.issued","2006"],["dc.description.abstract","Neurons and glial cells differentiate from common multipotent precursors in the vertebrate retina. We have identified a novel member of the hairy/Enhancer of split [E(spl)] gene family in Xenopus, XHes2, as a regulator to bias retinal precursor cells towards a glial fate. XHes2 expression is predominantly restricted to sensory organ territories, including the retina. Using in vivo lipofection in the optic vesicle, we found that XHes2 overexpression dramatically increases gliogenesis at the expense of neurogenesis. This increase in glial cells correlates with a delayed cell cycle withdrawal of some retinal progenitors. In addition, birthdating experiments suggest that XHes2 deviates some early born cell types towards a glial fate that would normally have given rise to neurons. Conversely, a significant inhibition of glial differentiation is observed upon XHes2 loss of function. The gliogenic activity of XHes2 relies on its ability to inhibit neuronal differentiation by at least two distinct mechanisms: it not only negatively regulates XNgnr1 and NeuroD transcription, but it also physically interacts with a subset of proneural bHLH proteins."],["dc.identifier.doi","10.1242/dev.02567"],["dc.identifier.isi","000240849300017"],["dc.identifier.pmid","17008450"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25557"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Company Of Biologists Ltd"],["dc.relation.issn","0950-1991"],["dc.title","Characterization and function of the bHLH-O protein XHes2: insight into the mechanisms controlling retinal cell fate decision"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","110"],["dc.bibliographiccitation.journal","BMC developmental biology"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Dullin, Jean-Philippe"],["dc.contributor.author","Locker, Morgane"],["dc.contributor.author","Robach, Mélodie"],["dc.contributor.author","Henningfeld, Kristine A."],["dc.contributor.author","Parain, Karine"],["dc.contributor.author","Afelik, Solomon"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2019-07-10T08:13:01Z"],["dc.date.available","2019-07-10T08:13:01Z"],["dc.date.issued","2007"],["dc.description.abstract","Background: In recent years, considerable knowledge has been gained on the molecular mechanisms underlying retinal cell fate specification. However, hitherto studies focused primarily on the six major retinal cell classes (five types of neurons of one type of glial cell), and paid little attention to the specification of different neuronal subtypes within the same cell class. In particular, the molecular machinery governing the specification of the two most abundant neurotransmitter phenotypes in the retina, GABAergic and glutamatergic, is largely unknown. In the spinal cord and cerebellum, the transcription factor Ptf1a is essential for GABAergic neuron production. In the mouse retina, Ptf1a has been shown to be involved in horizontal and most amacrine neurons differentiation.Results: In this study, we examined the distribution of neurotransmitter subtypes following Ptf1a gain and loss of function in the Xenopus retina. We found cell-autonomous dramatic switches between GABAergic and glutamatergic neuron production, concomitant with profound defects in the genesis of amacrine and horizontal cells, which are mainly GABAergic. Therefore, we investigated whether Ptf1a promotes the fate of these two cell types or acts directly as a GABAergic subtype determination factor. In ectodermal explant assays ... Conclusion: Altogether, our results reveal for the first time in the retina a major player in the GABAergic versus glutamatergic cell specification genetic pathway."],["dc.identifier.fs","87121"],["dc.identifier.ppn","559806469"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4372"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61102"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.subject.ddc","573.8"],["dc.subject.ddc","612"],["dc.subject.ddc","612.8"],["dc.title","Ptf1a triggers GABAergic neuronal cell fates in the retina"],["dc.title.alternative","Research article"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]