Pieler, Tomas

[["dc.bibliographiccitation.artnumber","36"],["dc.bibliographiccitation.journal","BMC Developmental Biology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Nieber, Frank"],["dc.contributor.author","Hedderich, Marie"],["dc.contributor.author","Jahn, Olaf"],["dc.contributor.author","Pieler, Tomas"],["dc.contributor.author","Henningfeld, Kristine A."],["dc.date.accessioned","2018-11-07T09:18:40Z"],["dc.date.available","2018-11-07T09:18:40Z"],["dc.date.issued","2013"],["dc.description.abstract","Background: Members of the vertebrate Numb family of cell fate determinants serve multiple functions throughout early embryogenesis, including an essential role in the development of the nervous system. The Numb proteins interact with various partner proteins and correspondingly participate in multiple cellular activities, including inhibition of the Notch pathway. Results: Here, we describe the expression characteristics of Numb and Numblike (NumbL) during Xenopus development and characterize the function of NumbL during primary neurogenesis. NumbL, in contrast to Numb, is expressed in the territories of primary neurogenesis and is positively regulated by the Neurogenin family of proneural transcription factors. Knockdown of NumbL afforded a complete loss of primary neurons and did not lead to an increase in Notch signaling in the open neural plate. Furthermore, we provide evidence that interaction of NumbL with the AP-2 complex is required for NumbL function during primary neurogenesis. Conclusion: We demonstrate an essential role of NumbL during Xenopus primary neurogenesis and provide evidence for a Notch-independent function of NumbL in this context."],["dc.identifier.doi","10.1186/1471-213X-13-36"],["dc.identifier.isi","000325803700001"],["dc.identifier.pmid","24125469"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10415"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28454"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-213X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","NumbL is essential for Xenopus primary neurogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3777"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.lastpage","3787"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Claussen, Maike"],["dc.contributor.author","Lingner, Thomas"],["dc.contributor.author","Pommerenke, Claudia"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2018-11-07T09:49:18Z"],["dc.date.available","2018-11-07T09:49:18Z"],["dc.date.issued","2015"],["dc.description.abstract","RNAs that localize to the vegetal cortex during Xenopus laevis oogenesis have been reported to function in germ layer patterning, axis determination, and development of the primordial germ cells. Here we report on the genome-wide, comparative analysis of differentially localizing RNAs in Xenopus laevis and Xenopus tropicalis oocytes, revealing a surprisingly weak degree of conservation in respect to the identity of animally as well as vegetally enriched transcripts in these closely related species. Heterologous RNA injections and protein binding studies indicate that the different RNA localization patterns in these two species are due to gain/loss of cis-acting localization signals rather than to differences in the RNA-localizing machinery."],["dc.identifier.doi","10.1091/mbc.E15-02-0115"],["dc.identifier.isi","000366322200015"],["dc.identifier.pmid","26337391"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12732"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35480"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.relation.issn","1939-4586"],["dc.relation.issn","1059-1524"],["dc.rights","CC BY-NC-SA 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-sa/3.0"],["dc.title","Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","413"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Genes"],["dc.bibliographiccitation.lastpage","426"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Borchers, Annette"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2019-07-09T11:40:12Z"],["dc.date.available","2019-07-09T11:40:12Z"],["dc.date.issued","2010"],["dc.description.abstract","Xenopus embryos provide a rich source of pluripotent cells that can be differentiated into functional organs. Since the molecular principles of vertebrate organogenesis appear to be conserved between Xenopus and mammals, this system can provide useful guidelines for the directional manipulation of human embryonic stem cells. Pluripotent Xenopus cells can be easily isolated from the animal pole of blastula stage Xenopus embryos. These so called \"animal cap\" cells represent prospective ectodermal cells, but give rise to endodermal, mesodermal and neuro-ectodermal derivatives if treated with the appropriate factors. These factors include evolutionary conserved modulators of the key developmental signal transduction pathways that can be supplied either by mRNA microinjection or direct application of recombinant proteins. This relatively simple system has added to our understanding of pancreas, liver, kidney, eye and heart development. In particular, recent studies have used animal cap cells to generate ectopic eyes and hearts, setting the stage for future work aimed at programming pluripotent cells for regenerative medicine."],["dc.identifier.doi","10.3390/genes1030413"],["dc.identifier.fs","598786"],["dc.identifier.pmid","24710095"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10735"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58111"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2073-4425"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Programming pluripotent precursor cells derived from Xenopus embryos to generate specific tissues and organs."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2347"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Genes & Development"],["dc.bibliographiccitation.lastpage","2352"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Souopgui, Jacob"],["dc.contributor.author","Rust, Barbara"],["dc.contributor.author","Vanhomwegen, Jessica"],["dc.contributor.author","Heasman, Janet"],["dc.contributor.author","Henningfeld, Kristine A."],["dc.contributor.author","Bellefroid, Eric J."],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2018-11-07T11:11:28Z"],["dc.date.available","2018-11-07T11:11:28Z"],["dc.date.issued","2008"],["dc.description.abstract","VegT represents a localized maternal determinant essentially required for endoderm formation in Xenopus. Here, we report on the identification of the RNA-binding protein XSeb4R as a positive regulator of VegT. XSeb4R interacts directly with the 3'-untranslated region of VegT mRNA, stabilizes it, and stimulates translation. Ablation of XSeb4R activity results in impairment of endoderm and mesoderm formation, while ectopic expression of XSeb4R in ectodermal cells induces endodermal and mesodermal gene expression. These observations unravel a novel mode of VegT regulation at the post-transcriptional level that is essential for germ layer formation in Xenopus."],["dc.identifier.doi","10.1101/gad.479808"],["dc.identifier.isi","000258878100006"],["dc.identifier.pmid","18765788"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6117"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53443"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cold Spring Harbor Lab Press, Publications Dept"],["dc.relation.issn","0890-9369"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The RNA-binding protein XSeb4R: a positive regulator of VegT mRNA stability and translation that is required for germ layer formation in Xenopus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Cell and Developmental Biology"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Hahn, Anne"],["dc.contributor.author","Pensold, Daniel"],["dc.contributor.author","Bayer, Cathrin"],["dc.contributor.author","Tittelmeier, Jessica"],["dc.contributor.author","González-Bermúdez, Lourdes"],["dc.contributor.author","Marx-Blümel, Lisa"],["dc.contributor.author","Linde, Jenice"],["dc.contributor.author","Groß, Jonas"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Lingner, Thomas"],["dc.contributor.author","von Maltzahn, Julia"],["dc.contributor.author","Spehr, Marc"],["dc.contributor.author","Pieler, Tomas"],["dc.contributor.author","Urbach, Anja"],["dc.contributor.author","Zimmer-Bensch, Geraldine"],["dc.date.accessioned","2021-04-14T08:23:52Z"],["dc.date.available","2021-04-14T08:23:52Z"],["dc.date.issued","2020"],["dc.description.abstract","Increased life expectancy in modern society comes at the cost of age-associated disabilities and diseases. Aged brains not only show reduced excitability and plasticity, but also a decline in inhibition. Age-associated defects in inhibitory circuits likely contribute to cognitive decline and age-related disorders. Molecular mechanisms that exert epigenetic control of gene expression contribute to age-associated neuronal impairments. Both DNA methylation, mediated by DNA methyltransferases (DNMTs), and histone modifications maintain neuronal function throughout lifespan. Here we provide evidence that DNMT1 function is implicated in the age-related loss of cortical inhibitory interneurons. Dnmt1 deletion in parvalbumin-positive interneurons attenuates their age-related decline in the cerebral cortex. Moreover, conditional Dnmt1-deficient mice show improved somatomotor performance and reduced aging-associated transcriptional changes. A decline in the proteostasis network, responsible for the proper degradation and removal of defective proteins, is implicated in age- and disease-related neurodegeneration. Our data suggest that DNMT1 acts indirectly on interneuron survival in aged mice by modulating the proteostasis network during life-time."],["dc.identifier.doi","10.3389/fcell.2020.00639"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81079"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","2296-634X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","DNA Methyltransferase 1 (DNMT1) Function Is Implicated in the Age-Related Loss of Cortical Interneurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1279"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Biology Open"],["dc.bibliographiccitation.lastpage","1287"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Dzementsei, Aliaksandr"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2019-07-09T11:40:03Z"],["dc.date.available","2019-07-09T11:40:03Z"],["dc.date.issued","2013"],["dc.description.abstract","The directional migration of primordial germ cells (PGCs) to the site of gonad formation is an advantageous model system to study cell motility. The embryonic development of PGCs has been investigated in different animal species, including mice, zebrafish, Xenopus and Drosophila. In this study we focus on the physical properties of Xenopus laevis PGCs during their transition from the passive to the active migratory state. Pre-migratory PGCs from Xenopus laevis embryos at developmental stages 17-19 to be compared with migratory PGCs from stages 28-30 were isolated and characterized in respect to motility and adhesive properties. Using single-cell force spectroscopy, we observed a decline in adhesiveness of PGCs upon reaching the migratory state, as defined by decreased attachment to extracellular matrix components like fibronectin, and a reduced adhesion to somatic endodermal cells. Data obtained from qPCR analysis with isolated PGCs reveal that down-regulation of E-cadherin might contribute to this weakening of cell-cell adhesion. Interestingly, however, using an in vitro migration assay, we found that movement of X. laevis PGCs can also occur independently of specific interactions with their neighboring cells. The reduction of cellular adhesion during PGC development is accompanied by enhanced cellular motility, as reflected in increased formation of bleb-like protrusions and inferred from electric cell-substrate impedance sensing (ECIS) as well as time-lapse image analysis. Temporal alterations in cell shape, including contraction and expansion of the cellular body, reveal a higher degree of cellular dynamics for the migratory PGCs in vitro."],["dc.identifier.doi","10.1242/bio.20135140"],["dc.identifier.fs","600738"],["dc.identifier.pmid","24285703"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10614"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58081"],["dc.language.iso","en"],["dc.relation.issn","2046-6390"],["dc.rights.access","openAccess"],["dc.title","Migratory and adhesive properties of Xenopus laevis primordial germ cells in vitro."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","110"],["dc.bibliographiccitation.journal","BMC developmental biology"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Dullin, Jean-Philippe"],["dc.contributor.author","Locker, Morgane"],["dc.contributor.author","Robach, Mélodie"],["dc.contributor.author","Henningfeld, Kristine A."],["dc.contributor.author","Parain, Karine"],["dc.contributor.author","Afelik, Solomon"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2019-07-10T08:13:01Z"],["dc.date.available","2019-07-10T08:13:01Z"],["dc.date.issued","2007"],["dc.description.abstract","Background: In recent years, considerable knowledge has been gained on the molecular mechanisms underlying retinal cell fate specification. However, hitherto studies focused primarily on the six major retinal cell classes (five types of neurons of one type of glial cell), and paid little attention to the specification of different neuronal subtypes within the same cell class. In particular, the molecular machinery governing the specification of the two most abundant neurotransmitter phenotypes in the retina, GABAergic and glutamatergic, is largely unknown. In the spinal cord and cerebellum, the transcription factor Ptf1a is essential for GABAergic neuron production. In the mouse retina, Ptf1a has been shown to be involved in horizontal and most amacrine neurons differentiation.Results: In this study, we examined the distribution of neurotransmitter subtypes following Ptf1a gain and loss of function in the Xenopus retina. We found cell-autonomous dramatic switches between GABAergic and glutamatergic neuron production, concomitant with profound defects in the genesis of amacrine and horizontal cells, which are mainly GABAergic. Therefore, we investigated whether Ptf1a promotes the fate of these two cell types or acts directly as a GABAergic subtype determination factor. In ectodermal explant assays ... Conclusion: Altogether, our results reveal for the first time in the retina a major player in the GABAergic versus glutamatergic cell specification genetic pathway."],["dc.identifier.fs","87121"],["dc.identifier.ppn","559806469"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4372"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61102"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.subject.ddc","573.8"],["dc.subject.ddc","612"],["dc.subject.ddc","612.8"],["dc.title","Ptf1a triggers GABAergic neuronal cell fates in the retina"],["dc.title.alternative","Research article"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","16148"],["dc.bibliographiccitation.issue","37"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","16153"],["dc.bibliographiccitation.volume","107"],["dc.contributor.author","Koebernick, Katja"],["dc.contributor.author","Loeber, Jana"],["dc.contributor.author","Arthur, Patrick Kobina"],["dc.contributor.author","Tarbashevich, Katsiaryna"],["dc.contributor.author","Pieler, Tomas"],["dc.date.accessioned","2018-11-07T08:39:12Z"],["dc.date.available","2018-11-07T08:39:12Z"],["dc.date.issued","2010"],["dc.description.abstract","Segregation of the future germline defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus embryos. The part of XDE-LE critical for somatic RNA clearance exhibits homology to zebrafish nanos1 and appears to be targeted by Xenopus miR-18 for somatic mRNA clearance. Xenopus Elr-type proteins of the vegetal localization complex can alleviate somatic RNA clearance of microinjected XDE-LE and endogenous XDE mRNA. ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [Pi159/9-1]"],["dc.identifier.doi","10.1073/pnas.1004401107"],["dc.identifier.isi","000281799000033"],["dc.identifier.pmid","20805475"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6118"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18939"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]