Poehlein, Anja

[["dc.bibliographiccitation.firstpage","1500"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Microorganisms"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Salar-Vidal, Llanos"],["dc.contributor.author","Achermann, Yvonne"],["dc.contributor.author","Aguilera-Correa, John-Jairo"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Esteban, Jaime"],["dc.contributor.author","Brüggemann, Holger"],["dc.contributor.authorgroup","on behalf of the ESCMID Study Group for Implant-Associated Infections (ESGIAI)"],["dc.date.accessioned","2021-09-01T06:43:04Z"],["dc.date.available","2021-09-01T06:43:04Z"],["dc.date.issued","2021"],["dc.description.abstract","Cutibacterium acnes is a common cause of prosthetic joint infections (PJIs). The C. acnes population can be divided into six main phylotypes (IA1, IA2, IB, IC, II and III) that are associated with different clinical conditions and normal skin. A single-locus sequence typing (SLST) scheme can distinguish ten main SLST types: A-E (all IA1), F (IA2), G (IC), H (IB), K (II), L (III). We genome-sequenced and compared 16 strains of C. acnes isolated from healthy skin (n = 4) and PJIs (n = 12), including six PJI cases with a good outcome (four shoulder PJIs, one hip PJI, one knee PJI) and six with infection relapse (three shoulder PJIs, three hip PJIs). The sequenced strains belonged to four different phylotypes (IA1, IA2, IB and II) and seven different SLST types. All five type IB strains (all SLST type H1) were PJI isolates (three hip PJIs, two shoulder PJIs), and four of these caused infection relapse (three hip PJIs, one shoulder PJI). Isolates from PJI cases with a good outcome belonged to three different phylotypes (IA, IB, II). Interestingly, four strains (three strains from PJI cases with good outcome and one strain from healthy skin) contained a linear plasmid; these strains belonged to different SLST types (A1, C1, F4, H1) and were isolated in three different hospitals. This study suggests that type IB strains have the potential to cause infection relapse, in particular regarding hip PJIs. Moreover, our study revealed that strains belonging to the same SLST type can differ in their accessory genome in different geographic locations, indicative of microevolution."],["dc.description.abstract","Cutibacterium acnes is a common cause of prosthetic joint infections (PJIs). The C. acnes population can be divided into six main phylotypes (IA1, IA2, IB, IC, II and III) that are associated with different clinical conditions and normal skin. A single-locus sequence typing (SLST) scheme can distinguish ten main SLST types: A-E (all IA1), F (IA2), G (IC), H (IB), K (II), L (III). We genome-sequenced and compared 16 strains of C. acnes isolated from healthy skin (n = 4) and PJIs (n = 12), including six PJI cases with a good outcome (four shoulder PJIs, one hip PJI, one knee PJI) and six with infection relapse (three shoulder PJIs, three hip PJIs). The sequenced strains belonged to four different phylotypes (IA1, IA2, IB and II) and seven different SLST types. All five type IB strains (all SLST type H1) were PJI isolates (three hip PJIs, two shoulder PJIs), and four of these caused infection relapse (three hip PJIs, one shoulder PJI). Isolates from PJI cases with a good outcome belonged to three different phylotypes (IA, IB, II). Interestingly, four strains (three strains from PJI cases with good outcome and one strain from healthy skin) contained a linear plasmid; these strains belonged to different SLST types (A1, C1, F4, H1) and were isolated in three different hospitals. This study suggests that type IB strains have the potential to cause infection relapse, in particular regarding hip PJIs. Moreover, our study revealed that strains belonging to the same SLST type can differ in their accessory genome in different geographic locations, indicative of microevolution."],["dc.description.sponsorship","Fabrikant Vilhelm Pedersen og Hustrus Legat"],["dc.identifier.doi","10.3390/microorganisms9071500"],["dc.identifier.pii","microorganisms9071500"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89211"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-2607"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Genomic Analysis of Cutibacterium acnes Strains Isolated from Prosthetic Joint Infections"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","332"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Pathogens"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Migliorini, Letícia B."],["dc.contributor.author","de Sales, Romário O."],["dc.contributor.author","Koga, Paula C. M."],["dc.contributor.author","Doi, Andre M."],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Toniolo, Alexandra R."],["dc.contributor.author","Menezes, Fernando G."],["dc.contributor.author","Martino, Marines D. V."],["dc.contributor.author","Gales, Ana C."],["dc.contributor.author","Brüggemann, Holger"],["dc.contributor.author","Severino, Patricia"],["dc.date.accessioned","2021-04-14T08:27:52Z"],["dc.date.available","2021-04-14T08:27:52Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Fundação de Amparo à Pesquisa do Estado de São Paulo"],["dc.identifier.doi","10.3390/pathogens10030332"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82433"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-0817"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Prevalence of blaKPC-2, blaKPC-3 and blaKPC-30—Carrying Plasmids in Klebsiella pneumoniae Isolated in a Brazilian Hospital"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.affiliation","Afshar, Mastaneh; 1Department of Biomedicine, Aarhus University, Aarhus, Denmark"],["dc.contributor.affiliation","Møllebjerg, Andreas; 2Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark"],["dc.contributor.affiliation","Minero, Gabriel Antonio; 2Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark"],["dc.contributor.affiliation","Hollensteiner, Jacqueline; 3Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, University of Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Poehlein, Anja; 3Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, University of Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Himmelbach, Axel; 4Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany"],["dc.contributor.affiliation","Lange, Jeppe; 5Department of Clinical Medicine, Aarhus University, Aarhus, Denmark"],["dc.contributor.affiliation","Meyer, Rikke Louise; 2Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark"],["dc.contributor.affiliation","Brüggemann, Holger; 1Department of Biomedicine, Aarhus University, Aarhus, Denmark"],["dc.contributor.author","Afshar, Mastaneh"],["dc.contributor.author","Møllebjerg, Andreas"],["dc.contributor.author","Minero, Gabriel Antonio"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Himmelbach, Axel"],["dc.contributor.author","Lange, Jeppe"],["dc.contributor.author","Meyer, Rikke Louise"],["dc.contributor.author","Brüggemann, Holger"],["dc.date.accessioned","2022-12-12T08:15:00Z"],["dc.date.available","2022-12-12T08:15:00Z"],["dc.date.issued","2022-11-28"],["dc.date.updated","2022-12-12T08:13:36Z"],["dc.description.abstract","Staphylococcus saccharolyticus, a coagulase-negative staphylococcal species, has some unusual characteristics for human-associated staphylococci, such as slow growth and its preference for anoxic culture conditions. This species is a relatively abundant member of the human skin microbiota, but its microbiological properties, as well as the pathogenic potential, have scarcely been investigated so far, despite being occasionally isolated from different types of infections including orthopedic implant-associated infections. Here, we investigated the growth and biofilm properties of clinical isolates of S. saccharolyticus and determined host cell responses. Growth assessments in anoxic and oxic conditions revealed strain-dependent outcomes, as some strains can also grow aerobically. All tested strains of S. saccharolyticus were able to form biofilm in a microtiter plate assay. Strain-dependent differences were determined by optical coherence tomography, revealing that medium supplementation with glucose and sodium chloride enhanced biofilm formation. Visualization of the biofilm by confocal laser scanning microscopy revealed the role of extracellular DNA in the biofilm structure. In addition to attached biofilms, S. saccharolyticus also formed bacterial aggregates at an early stage of growth. Transcriptome analysis of biofilm-grown versus planktonic cells revealed a set of upregulated genes in biofilm-embedded cells, including factors involved in adhesion, colonization, and competition such as epidermin, type I toxin-antitoxin system, and phenol-soluble modulins (beta and epsilon). To investigate consequences for the host after encountering S. saccharolyticus, cytokine profiling and host cell viability were assessed by infection experiments with differentiated THP-1 cells. The microorganism strongly triggered the secretion of the tested pro-inflammatory cyto- and chemokines IL-6, IL-8, and TNF-alpha, determined at 24 h post-infection. S. saccharolyticus was less cytotoxic than Staphylococcus aureus. Taken together, the results indicate that S. saccharolyticus has substantial pathogenic potential. Thus, it can be a potential cause of orthopedic implant-associated infections and other types of deep-seated infections."],["dc.identifier.doi","10.3389/fmicb.2022.1070201"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118500"],["dc.language.iso","en"],["dc.relation.eissn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Biofilm formation and inflammatory potential of Staphylococcus saccharolyticus: A possible cause of orthopedic implant-associated infections"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Brüggemann, Holger"],["dc.contributor.author","Jensen, Anders"],["dc.contributor.author","Nazipi, Seven"],["dc.contributor.author","Aslan, Hüsnü"],["dc.contributor.author","Meyer, Rikke Louise"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Brzuszkiewicz, Elzbieta"],["dc.contributor.author","Al-Zeer, Munir A."],["dc.contributor.author","Brinkmann, Volker"],["dc.contributor.author","Söderquist, Bo"],["dc.date.accessioned","2020-12-10T18:10:08Z"],["dc.date.available","2020-12-10T18:10:08Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1038/s41598-017-18661-8"],["dc.identifier.eissn","2045-2322"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15413"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73859"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Pan-genome analysis of the genus Finegoldia identifies two distinct clades, strain-specific heterogeneity, and putative virulence factors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","221"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Microbiology"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Ahle, Charlotte M."],["dc.contributor.author","Stødkilde-Jørgensen, Kristian"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Streit, Wolfgang R."],["dc.contributor.author","Hüpeden, Jennifer"],["dc.contributor.author","Brüggemann, Holger"],["dc.date.accessioned","2021-11-25T11:03:23Z"],["dc.date.accessioned","2022-08-18T12:35:21Z"],["dc.date.available","2021-11-25T11:03:23Z"],["dc.date.available","2022-08-18T12:35:21Z"],["dc.date.issued","2021-07-28"],["dc.date.updated","2022-07-29T12:07:23Z"],["dc.description.abstract","Abstract\r\n \r\n Background\r\n Staphylococci are important members of the human skin microbiome. Many staphylococcal species and strains are commensals of the healthy skin microbiota, while few play essential roles in skin diseases such as atopic dermatitis. To study the involvement of staphylococci in health and disease, it is essential to determine staphylococcal populations in skin samples beyond the genus and species level. Culture-independent approaches such as amplicon next-generation sequencing (NGS) are time- and cost-effective options. However, their suitability depends on the power of resolution.\r\n \r\n \r\n Results\r\n Here we compare three amplicon NGS schemes that rely on different targets within the genes tuf and rpsK, designated tuf1, tuf2 and rpsK schemes. The schemes were tested on mock communities and on human skin samples. To obtain skin samples and build mock communities, skin swab samples of healthy volunteers were taken. In total, 254 staphylococcal strains were isolated and identified to the species level by MALDI-TOF mass spectrometry. A subset of ten strains belonging to different staphylococcal species were genome-sequenced. Two mock communities with nine and eighteen strains, respectively, as well as eight randomly selected skin samples were analysed with the three amplicon NGS methods. Our results imply that all three methods are suitable for species-level determination of staphylococcal populations. However, the novel tuf2-NGS scheme was superior in resolution power. It unambiguously allowed identification of Staphylococcus saccharolyticus and distinguish phylogenetically distinct clusters of Staphylococcus epidermidis.\r\n \r\n \r\n Conclusions\r\n Powerful amplicon NGS approaches for the detection and relative quantification of staphylococci in human samples exist that can resolve populations to the species and, to some extent, to the subspecies level. Our study highlights strengths, weaknesses and pitfalls of three currently available amplicon NGS approaches to determine staphylococcal populations. Applied to the analysis of healthy and diseased skin, these approaches can be useful to attribute host-beneficial and -detrimental roles to skin-resident staphylococcal species and subspecies."],["dc.identifier.citation","BMC Microbiology. 2021 Jul 28;21(1):221"],["dc.identifier.doi","10.1186/s12866-021-02284-1"],["dc.identifier.pii","2284"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/93522"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112939"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.publisher","BioMed Central"],["dc.relation.eissn","1471-2180"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.title","Comparison of three amplicon sequencing approaches to determine staphylococcal populations on human skin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2241"],["dc.bibliographiccitation.journal","Frontiers in microbiology"],["dc.bibliographiccitation.lastpage","2241"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Davidsson, Sabina"],["dc.contributor.author","Carlsson, Jessica"],["dc.contributor.author","Mölling, Paula"],["dc.contributor.author","Gashi, Natyra"],["dc.contributor.author","Andrén, Ove"],["dc.contributor.author","Andersson, Swen-Olof"],["dc.contributor.author","Brzuszkiewicz, Elzbieta"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Al-Zeer, Munir A"],["dc.contributor.author","Brinkmann, Volker"],["dc.contributor.author","Scavenius, Carsten"],["dc.contributor.author","Nazipi, Seven"],["dc.contributor.author","Söderquist, Bo"],["dc.contributor.author","Brüggemann, Holger"],["dc.date.accessioned","2019-07-09T11:44:46Z"],["dc.date.available","2019-07-09T11:44:46Z"],["dc.date.issued","2017"],["dc.description.abstract","Inflammation is one of the hallmarks of prostate cancer. The origin of inflammation is unknown, but microbial infections are suspected to play a role. In previous studies, the Gram-positive, low virulent bacterium Cutibacterium (formerly Propionibacterium) acnes was frequently isolated from prostatic tissue. It is unclear if the presence of the bacterium represents a true infection or a contamination. Here we investigated Cutibacterium acnes type II, also called subspecies defendens, which is the most prevalent type among prostatic C. acnes isolates. Genome sequencing of type II isolates identified large plasmids in several genomes. The plasmids are highly similar to previously identified linear plasmids of type I C. acnes strains associated with acne vulgaris. A PCR-based analysis revealed that 28.4% (21 out of 74) of all type II strains isolated from cancerous prostates carry a plasmid. The plasmid shows signatures for conjugative transfer. In addition, it contains a gene locus for tight adherence (tad) that is predicted to encode adhesive Flp (fimbrial low-molecular weight protein) pili. In subsequent experiments a tad locus-encoded putative pilin subunit was identified in the surface-exposed protein fraction of plasmid-positive C. acnes type II strains by mass spectrometry, indicating that the tad locus is functional. Additional plasmid-encoded proteins were detected in the secreted protein fraction, including two signal peptide-harboring proteins; the corresponding genes are specific for type II C. acnes, thus lacking from plasmid-positive type I C. acnes strains. Further support for the presence of Flp pili in C. acnes type II was provided by electron microscopy, revealing cell appendages in tad locus-positive strains. Our study provides new insight in the most prevalent prostatic subspecies of C. acnes, subsp. defendens, and indicates the existence of Flp pili in plasmid-positive strains. Such pili may support colonization and persistent infection of human prostates by C. acnes."],["dc.identifier.doi","10.3389/fmicb.2017.02241"],["dc.identifier.pmid","29201018"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14897"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59092"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","397"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Pathogens"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Söderquist, Bo"],["dc.contributor.author","Afshar, Mastaneh"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Brüggemann, Holger"],["dc.date.accessioned","2021-06-01T09:42:40Z"],["dc.date.available","2021-06-01T09:42:40Z"],["dc.date.issued","2021"],["dc.description.abstract","The anaerobic coagulase-negative staphylococcal species Staphylococcus saccharolyticus is a member of the normal skin microbiota. However, S. saccharolyticus is rarely found in clinical specimens and its pathogenic potential is unclear. The clinical data of prosthetic hip (n = 5) and shoulder (n = 2) joint implant-associated infections where S. saccharolyticus was detected in periprosthetic tissue specimens are described. The prosthetic hip joint infection cases presented as “aseptic” loosening and may represent chronic, insidious, low-grade prosthetic joint infections (PJIs), eventually resulting in loosening of prosthetic components. All cases were subjected to one-stage revision surgery and the long-term outcome was good. The shoulder joint infections had an acute onset. Polymicrobial growth, in all cases with Cutibacterium acnes, was found in 4/7 patients. All but one case were treated with long-term administration of beta-lactam antibiotics. Whole-genome sequencing (WGS) of the isolates was performed and potential virulence traits were identified. WGS could distinguish two phylogenetic clades (clades 1 and 2), which likely represent distinct subspecies of S. saccharolyticus. Little strain individuality was observed among strains from the same clade. Strains of clade 2 were exclusively associated with hip PJIs, whereas clade 1 strains originated from shoulder PJIs. It is possible that strains of the two clades colonize different skin habitats. In conclusion, S. saccharolyticus has the potential to cause PJIs that were previously regarded as aseptic loosening of prosthetic joint devices."],["dc.description.sponsorship","Nyckelfonden at Örebro University Hospital (OLL- 334 595951) and the Fabrikant Vilhelm Pedersen og Hustrus Legat (on the recommendation of the 335 Novo Nordisk Foundation)."],["dc.identifier.doi","10.3390/pathogens10040397"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85316"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-0817"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Staphylococcus saccharolyticus Associated with Prosthetic Joint Infections: Clinical Features and Genomic Characteristics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]