Poehlein, Anja

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Genome announcements"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Krabben, Preben"],["dc.contributor.author","Dürre, Peter"],["dc.contributor.author","Daniel, Rolf"],["dc.date.accessioned","2015-10-12T14:49:01Z"],["dc.date.accessioned","2021-10-27T13:11:22Z"],["dc.date.available","2015-10-12T14:49:01Z"],["dc.date.available","2021-10-27T13:11:22Z"],["dc.date.issued","2014"],["dc.description.abstract","Clostridium saccharoperbutylacetonicum strain DSM 14923 is known as a butanol-producing bacterium. Various organic compounds such as glucose, fructose, sucrose, mannose, and cellobiose are fermented. The genome consists of one chromosome and one circular megaplasmid. C. saccharoperbutylacetonicum was used in industrial fermentation processes to produce the solvents acetone, butanol, and ethanol."],["dc.identifier.doi","10.1128/genomeA.01056-14"],["dc.identifier.fs","609807"],["dc.identifier.pmid","25323722"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12154"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91590"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.issn","2169-8287"],["dc.relation.orgunit","Fakultät für Biologie und Psychologie"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Complete Genome Sequence of the Solvent Producer Clostridium saccharoperbutylacetonicum Strain DSM 14923."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Genetics"],["dc.bibliographiccitation.volume","13"],["dc.contributor.affiliation","Baur, Saskia Tabea; \n1\nInstitute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany"],["dc.contributor.affiliation","Poehlein, Anja; \n2\nDepartment of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Renz, Niklas Jan; \n1\nInstitute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany"],["dc.contributor.affiliation","Hollitzer, Stefanie Karolina; \n1\nInstitute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany"],["dc.contributor.affiliation","Montoya Solano, José David; \n1\nInstitute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany"],["dc.contributor.affiliation","Schiel-Bengelsdorf, Bettina; \n1\nInstitute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany"],["dc.contributor.affiliation","Daniel, Rolf; \n2\nDepartment of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Dürre, Peter; \n1\nInstitute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany"],["dc.contributor.author","Baur, Saskia Tabea"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Renz, Niklas Jan"],["dc.contributor.author","Hollitzer, Stefanie Karolina"],["dc.contributor.author","Montoya Solano, José David"],["dc.contributor.author","Schiel-Bengelsdorf, Bettina"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.date.accessioned","2022-08-25T06:53:36Z"],["dc.date.available","2022-08-25T06:53:36Z"],["dc.date.issued","2022-08-11"],["dc.date.updated","2022-08-25T05:51:33Z"],["dc.description.abstract","Solvents such as butanol are important platform chemicals and are often produced from petrochemical sources. Production of butanol and other compounds from renewable and sustainable resources can be achieved by solventogenic bacteria, such as the hyper-butanol producer Clostridium saccharoperbutylacetonicum. Its sol operon consists of the genes encoding butyraldehyde dehydrogenase, CoA transferase, and acetoacetate decarboxylase (bld, ctfA, ctfB, adc) and the gene products are involved in butanol and acetone formation. It is important to understand its regulation to further optimize the solvent production. In this study, a new long non-coding antisense transcript complementary to the complete sol operon, now called Assolrna, was identified by transcriptomic analysis and the regulatory mechanism of Assolrna was investigated. For this purpose, the promoter-exchange strain C. saccharoperbutylacetonicum ΔP\r\nasr\r\n\r\n::P\r\nasr\r\n\r\n** was constructed. Additionally, Assolrna was expressed plasmid-based under control of the native P\r\nasr\r\n promoter and the lactose-inducible P\r\nbgaL\r\n promoter in both the wild type and the promoter-exchange strain. Solvent formation was strongly decreased for all strains based on C. saccharoperbutylacetonicum ΔP\r\nasr\r\n\r\n::P\r\nasr\r\n\r\n** and growth could not be restored by plasmid-based complementation of the exchanged promoter. Interestingly, very little sol mRNA expression was detected in the strain C. saccharoperbutylacetonicum ΔP\r\nasr\r\n\r\n::P\r\nasr\r\n\r\n** lacking Assolrna expression. Butanol titers were further increased for the overexpression strain C. saccharoperbutylacetonicum [pMTL83151_asr_P\r\nbgaL\r\n] compared to the wild type. These results suggest that Assolrna has a positive effect on sol operon expression. Therefore, a possible stabilization mechanism of the sol mRNA by Assolrna under physiological concentrations is proposed."],["dc.identifier.doi","10.3389/fgene.2022.966643"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113218"],["dc.language.iso","en"],["dc.relation.eissn","1664-8021"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Modulation of sol mRNA expression by the long non-coding RNA Assolrna in Clostridium saccharoperbutylacetonicum affects solvent formation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","FEMS Microbiology Letters"],["dc.bibliographiccitation.volume","367"],["dc.contributor.author","Schüler, Miriam A"],["dc.contributor.author","Stegmann, Benjamin A"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.date.accessioned","2021-04-14T08:24:15Z"],["dc.date.available","2021-04-14T08:24:15Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1093/femsle/fnaa103"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81220"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1574-6968"],["dc.title","Genome sequence analysis of the temperate bacteriophage TBP2 of the solvent producer Clostridium saccharoperbutylacetonicum N1-4 (HMT, ATCC 27021)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Gabris, Christina"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Bengelsdorf, Frank R."],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.date.accessioned","2020-12-10T18:37:00Z"],["dc.date.available","2020-12-10T18:37:00Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1128/genomeA.01488-16"],["dc.identifier.eissn","2169-8287"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76811"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Genome Sequence of Enterococcus faecalis Strain CG_E"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.issue","30"],["dc.bibliographiccitation.journal","Standards in Genomic Sciences"],["dc.bibliographiccitation.volume","12"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Jag, Vanessa"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Bengelsdorf, Frank R."],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.date.accessioned","2019-07-09T11:44:21Z"],["dc.date.available","2019-07-09T11:44:21Z"],["dc.date.issued","2017"],["dc.description.abstract","A nonmotile, Gram-positive bacterium that shows an elongated and branching cell shape was isolated from soil samples from the botanical garden of Ulm University, Ulm, Germany. Here, the isolation procedure, identification, genome sequencing and metabolic features of the strain are described. Phylogenetic analysis allowed to identify the isolated strain as Oerskovia enterophila. The genus Oerskovia belongs to the family Cellulomonadaceae within the order Actinomycetales. The length of cells of O. enterophila ranges from 1 μm to 15 μm, depending on the growth phase. In the exponential growth phase, cells show an elongated and branching shape, whereas cells break up to round or coccoid elements in the stationary growth phase. The 4,535,074 bp long genome consists of 85 contigs with 3918 protein-coding genes and 57 RNA genes. The isolated strain was shown to degrade numerous complex carbon sources such as cellulose, chitin, and starch, which can be found ubiquitously in nature. Moreover, analysis of the genomic sequence revealed the genetic potential to degrade these compounds."],["dc.format.extent","8"],["dc.identifier.doi","10.1186/s40793-017-0244-4"],["dc.identifier.pmid","28484582"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14727"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58997"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Genome sequencing and description of Oerskovia enterophila VJag, an agar- and cellulose-degrading bacterium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","International Journal of Systematic and Evolutionary Microbiology"],["dc.bibliographiccitation.volume","72"],["dc.contributor.author","Schoch, Teresa"],["dc.contributor.author","Höfele, Franziska"],["dc.contributor.author","Odeh, Hannah"],["dc.contributor.author","Winter, Lisa-Maria"],["dc.contributor.author","Stöferle, Sophia"],["dc.contributor.author","Karl, Michael"],["dc.contributor.author","Bengelsdorf, Frank"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.date.accessioned","2022-11-01T10:16:58Z"],["dc.date.available","2022-11-01T10:16:58Z"],["dc.date.issued","2022"],["dc.description.abstract","Clostridium aurantibutyricum\n \n \n ,\n \n \n Clostridium felsineum\n \n \n and\n \n \n Clostridium roseum\n \n \n share a very high similarity based on multi-locus sequence analysis. In this study, their correct taxonomic status was determined using genomic and phenotypic investigations. Average nucleotide identity based on MUMmer alignment of the genomes and\n in silico\n DNA–DNA hybridization resulted in values of 98.55–100 and 78.7–100 %, respectively, strongly indicating that all strains are members of the same species. In addition, morphological investigations, fatty acid analyses and substrate utilization tests revealed no striking differences between the strains. Therefore, we propose the reclassification of\n \n \n C. aurantibutyricum\n \n \n and\n \n \n C. roseum\n \n \n as later heterotypic synonyms of\n \n \n C. felsineum\n \n \n . The type strain is lodged in several culture collections (ATCC 17788\n T\n =DSM 794\n T\n =NCIMB 10690\n T\n )."],["dc.identifier.doi","10.1099/ijsem.0.005589"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116698"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.relation.eissn","1466-5034"],["dc.relation.issn","1466-5026"],["dc.title","Reclassification of Clostridium aurantibutyricum Hellinger 1944 and Clostridium roseum (ex McCoy and McClung 1935) Cato et al. 1988"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Bengelsdorf, Frank Robert"],["dc.contributor.author","Schiel-Bengelsdorf, Bettina"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.date.accessioned","2020-12-10T18:36:59Z"],["dc.date.available","2020-12-10T18:36:59Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1128/genomeA.01430-16"],["dc.identifier.eissn","2169-8287"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76808"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Genome Sequence of the Acetogenic Bacterium Acetobacterium wieringae DSM 1911 T"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Gabris, Christina"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Bengelsdorf, Frank R."],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.date.accessioned","2020-12-10T18:37:00Z"],["dc.date.available","2020-12-10T18:37:00Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1128/genomeA.01487-16"],["dc.identifier.eissn","2169-8287"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76810"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Genome Sequence of Lactobacillus sunkii Strain CG_D"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.affiliation","Redl, Stephanie; 1Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark"],["dc.contributor.affiliation","Poehlein, Anja; 2Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University, Göttingen, Germany"],["dc.contributor.affiliation","Esser, Carola; 3Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Ulm, Germany"],["dc.contributor.affiliation","Bengelsdorf, Frank R.; 3Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Ulm, Germany"],["dc.contributor.affiliation","Jensen, Torbjørn Ø.; 1Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark"],["dc.contributor.affiliation","Jendresen, Christian B.; 1Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark"],["dc.contributor.affiliation","Tindall, Brian J.; 4Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswick, Germany"],["dc.contributor.affiliation","Daniel, Rolf; 2Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University, Göttingen, Germany"],["dc.contributor.affiliation","Dürre, Peter; 3Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Ulm, Germany"],["dc.contributor.affiliation","Nielsen, Alex T.; 1Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark"],["dc.contributor.author","Redl, Stephanie"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Esser, Carola"],["dc.contributor.author","Bengelsdorf, Frank R."],["dc.contributor.author","Jensen, Torbjørn Ø."],["dc.contributor.author","Jendresen, Christian B."],["dc.contributor.author","Tindall, Brian J."],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.contributor.author","Nielsen, Alex T."],["dc.date.accessioned","2020-12-10T18:44:28Z"],["dc.date.available","2020-12-10T18:44:28Z"],["dc.date.issued","2020"],["dc.date.updated","2022-02-09T13:22:22Z"],["dc.description.abstract","Fermentation of gases provides a promising opportunity for the production of biochemicals from renewable resources, which has resulted in a growing interest in acetogenic bacteria. Thermophilic organisms provide potential advantages for the fermentation of, e.g., syngas into for example volatile compounds, and the thermophiles Moorella thermoacetica and Moorella thermoautotrophica have become model organisms of acetogenic metabolism. The justification for the recognition of the closely related species M. thermoautotrophica has, however, recently been disputed. In order to expand knowledge on the genus, we have here genome sequenced a total of 12 different M. thermoacetica and M. thermoautotrophica strains. From the sequencing results, it became clear that M. thermoautotrophica DSM 1974T consists of at least two different strains. Two different strains were isolated in Lyngby and Ulm from a DSM 1974T culture obtained from the DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswick, Germany). Phylogenetic analysis revealed a close relationship between all the sequenced genomes, suggesting that the two strains detected in the type strain of the species M. thermoautotrophica could not be distinguished at the species level from M. thermoacetica. Despite genetic similarities, differences in genomic features were observed between the strains. Differences in compounds that can serve as carbon and energy sources for selected strains were also identified. On the contrary, strain DSM 21394, currently still named M. thermoacetica, obviously represents a new Moorella species. In addition, based on genome analysis and comparison M. glycerini NMP, M. stamsii DSM 26217T, and M. perchloratireducens An10 cannot be distinguished at the species level. Thus, this comprehensive analysis provides a significantly increased knowledge of the genetic diversity of Moorella strains."],["dc.identifier.doi","10.3389/fmicb.2019.03070"],["dc.identifier.eissn","1664-302X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78468"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-302X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Karl, Michael M."],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Bengelsdorf, Frank R."],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dürre, Peter"],["dc.date.accessioned","2020-12-10T18:36:56Z"],["dc.date.available","2020-12-10T18:36:56Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1128/genomeA.00423-17"],["dc.identifier.eissn","2169-8287"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76792"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Complete Genome Sequence of the Autotrophic Acetogen Clostridium formicaceticum DSM 92T Using Nanopore and Illumina Sequencing Data"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]