Poehlein, Anja

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Witten, Anika"],["dc.contributor.author","Wellnitz, Olga"],["dc.contributor.author","Shpigel, Nahum"],["dc.contributor.author","Petzl, Wolfram"],["dc.contributor.author","Zerbe, Holm"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dobrindt, Ulrich"],["dc.date.accessioned","2020-12-10T18:36:57Z"],["dc.date.available","2020-12-10T18:36:57Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1128/genomeA.00753-16"],["dc.identifier.eissn","2169-8287"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76798"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Whole-Genome Draft Sequences of Six Commensal Fecal and Six Mastitis-Associated Escherichia coli Strains of Bovine Origin: TABLE 1"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","723"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Oehler, Dirk"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Mueller, Nicolai"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Gottschalk, Gerhard"],["dc.contributor.author","Schink, Bernhard"],["dc.date.accessioned","2018-11-07T09:02:11Z"],["dc.date.available","2018-11-07T09:02:11Z"],["dc.date.issued","2012"],["dc.description.abstract","Background: Thermacetogenium phaeum is a thermophilic strictly anaerobic bacterium oxidizing acetate to CO2 in syntrophic association with a methanogenic partner. It can also grow in pure culture, e.g., by fermentation of methanol to acetate. The key enzymes of homoacetate fermentation (Wood-Ljungdahl pathway) are used both in acetate oxidation and acetate formation. The obvious reversibility of this pathway in this organism is of specific interest since syntrophic acetate oxidation operates close to the energetic limitations of microbial life. Results: The genome of Th. phaeum is organized on a single circular chromosome and has a total size of 2,939,057 bp. It comprises 3.215 open reading frames of which 75% could be assigned to a gene function. The G+C content is 53.88 mol%. Many CRISPR sequences were found, indicating heavy phage attack in the past. A complete gene set for a phage was found in the genome, and indications of phage action could also be observed in culture. The genome contained all genes required for CO2 reduction through the Wood-Ljungdahl pathway, including two formyl tetrahydrofolate ligases, three carbon monoxide dehydrogenases, one formate hydrogenlyase complex, three further formate dehydrogenases, and three further hydrogenases. The bacterium contains a menaquinone MQ-7. No indications of cytochromes or Rnf complexes could be found in the genome. Conclusions: The information obtained from the genome sequence indicates that Th. phaeum differs basically from the three homoacetogenic bacteria sequenced so far, i.e., the sodium ion-dependent Acetobacterium woodii, the ethanol-producing Clostridium ljungdahlii, and the cytochrome-containing Moorella thermoacetica. The specific enzyme outfit of Th. phaeum obviously allows ATP formation both in acetate formation and acetate oxidation."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft; Bonn-Bad Godesberg, Germany"],["dc.identifier.doi","10.1186/1471-2164-13-723"],["dc.identifier.isi","000314606400002"],["dc.identifier.pmid","23259483"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8501"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24621"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Genome-guided analysis of physiological and morphological traits of the fermentative acetate oxidizer Thermacetogenium phaeum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","93"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Antimicrobial Chemotherapy"],["dc.bibliographiccitation.lastpage","97"],["dc.bibliographiccitation.volume","70"],["dc.contributor.author","Eidam, Christopher"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Michael, Geovana Brenner"],["dc.contributor.author","Kadlec, Kristina"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Sweeney, Michael T."],["dc.contributor.author","Murray, Robert W."],["dc.contributor.author","Watts, Jeffrey L."],["dc.contributor.author","Schwarz, Stephan K. W."],["dc.date.accessioned","2018-11-07T10:03:31Z"],["dc.date.available","2018-11-07T10:03:31Z"],["dc.date.issued","2015"],["dc.description.abstract","Objectives: The aim of this study was to identify and analyse the first integrative and conjugative element (ICE) from Mannheimia haemolytica, the major bacterial component of the bovine respiratory disease (BRD) complex. Methods: The novel ICEMh1 was discovered in the whole-genome sequence of M. haemolytica 42548 by sequence analysis and comparative genomics. Transfer of ICEMh1 was confirmed by conjugation into Pasteurella multocida recipient cells. Results: ICEMh1 has a size of 92345 bp and harbours 107 genes. It integrates into a chromosomal tRNA(Leu) copy. Within two resistance gene regions of similar to 7.4 and 3.3 kb, ICEMh1 harbours five genes, which confer resistance to streptomycin (strA and strB), kanamycin/neomycin (aphA1), tetracycline [tetR-tet(H)] and sulphonamides (sul2). ICEMh1 is related to the recently described ICEPmu1 and both ICEs seem to have evolved from a common ancestor. A region of ICEMh1 that is absent in ICEPmu1 was found in putative ICE regions of other M. haemolytica genomes, suggesting a recombination event between two ICEs. ICEMh1 transfers to P. multocida by conjugation, in which it also uses a tRNA(Leu) as the integration site. PCR assays and susceptibility testing confirmed the presence and activity of the ICEMh1-associated resistance genes in the P. multocida recipient. Conclusions: These findings showed that ICEs, with structurally variable resistance gene regions, are present in BRD-associated Pasteurellaceae, can easily spread across genus borders and enable the acquisition of multidrug resistance via a single horizontal gene transfer event. This poses a threat to efficient antimicrobial chemotherapy of BRD-associated bacterial pathogens."],["dc.identifier.doi","10.1093/jac/dku361"],["dc.identifier.isi","000350210800013"],["dc.identifier.pmid","25239467"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38487"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1460-2091"],["dc.relation.issn","0305-7453"],["dc.title","Analysis and comparative genomics of ICEMh1, a novel integrative and conjugative element (ICE) of Mannheimia haemolytica"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","56"],["dc.bibliographiccitation.journal","Standards in Genomic Sciences"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Ullrich, Sophie R."],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Voget, Sonja"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Tischler, Judith S."],["dc.contributor.author","Schloemann, Michael"],["dc.contributor.author","Muehling, Martin"],["dc.date.accessioned","2018-11-07T09:53:12Z"],["dc.date.available","2018-11-07T09:53:12Z"],["dc.date.issued","2015"],["dc.description.abstract","The tenacious association between strains of the heterotrophic alphaproteobacterial genus Acidiphilium and chemolithotrophic iron oxidizing bacteria has long been known. In this context the genome of the heterotroph Acidiphilium sp. JA12-A1, an isolate from an iron oxidizing mixed culture derived from a pilot plant for bioremediation of acid mine drainage, was determined with the aim to reveal metabolic properties that are fundamental for the syntrophic interaction between Acidiphilium sp. JA12-A1 and the co-occurring chemolithoautotrophic iron oxidizer. The genome sequence consists of 4.18 Mbp on 297 contigs and harbors 4015 protein-coding genes and 50 RNA genes. Additionally, the molecular and functional organization of the Acidiphilium sp. JA12-A1 draft genome was compared to those of the close relatives Acidiphilium cryptum JF-5, Acidiphilium multivorum AIU301 and Acidiphilium sp. PM DSM 24941. The comparative genome analysis underlines the close relationship between these strains and the highly similar metabolic potential supports the idea that other Acidiphilium strains play a similar role in various acid mine drainage communities. Nevertheless, in contrast to other closely related strains Acidiphilium sp. JA12-A1 may be able to take up phosphonates as an additional source of phosphor."],["dc.identifier.doi","10.1186/s40793-015-0040-y"],["dc.identifier.isi","000367992400001"],["dc.identifier.pmid","26380040"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12489"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36284"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1944-3277"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Permanent draft genome sequence of Acidiphilium sp JA12-A1"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","419"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Genes"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Djukic, Marvin"],["dc.contributor.author","Erler, Silvio"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Grossar, Daniela"],["dc.contributor.author","Charrière, Jean-Daniel"],["dc.contributor.author","Gauthier, Laurent"],["dc.contributor.author","Hartken, Denise"],["dc.contributor.author","Dietrich, Sascha"],["dc.contributor.author","Nacke, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Poehlein, Anja"],["dc.date.accessioned","2019-07-09T11:45:46Z"],["dc.date.available","2019-07-09T11:45:46Z"],["dc.date.issued","2018"],["dc.description.abstract","In Europe, approximately 84% of cultivated crop species depend on insect pollinators, mainly bees. Apis mellifera (the Western honey bee) is the most important commercial pollinator worldwide. The Gram-positive bacterium Melissococcus plutonius is the causative agent of European foulbrood (EFB), a global honey bee brood disease. In order to detect putative virulence factors, we sequenced and analyzed the genomes of 14 M. plutonius strains, including two reference isolates. The isolates do not show a high diversity in genome size or number of predicted protein-encoding genes, ranging from 2.021 to 2.101 Mbp and 1589 to 1686, respectively. Comparative genomics detected genes that might play a role in EFB pathogenesis and ultimately in the death of the honey bee larvae. These include bacteriocins, bacteria cell surface- and host cell adhesion-associated proteins, an enterococcal polysaccharide antigen, an epsilon toxin, proteolytic enzymes, and capsule-associated proteins. In vivo expression of three putative virulence factors (endo-alpha-N-acetylgalactosaminidase, enhancin and epsilon toxin) was verified using naturally infected larvae. With our strain collection, we show for the first time that genomic differences exist between non-virulent and virulent typical strains, as well as a highly virulent atypical strain, that may contribute to the virulence of M. plutonius. Finally, we also detected a high number of conserved pseudogenes (75 to 156) per genome, which indicates genomic reduction during evolutionary host adaptation."],["dc.identifier.doi","10.3390/genes9080419"],["dc.identifier.pmid","30127293"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15313"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59307"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2073-4425"],["dc.relation.issn","2073-4425"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Comparative Genomics and Description of Putative Virulence Factors of Melissococcus plutonius, the Causative Agent of European Foulbrood Disease in Honey Bees"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","40"],["dc.bibliographiccitation.journal","Standards in Genomic Sciences"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Riegel, Karin"],["dc.contributor.author","Koenig, Sandra M."],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Duerre, Peter"],["dc.date.accessioned","2018-11-07T09:54:30Z"],["dc.date.available","2018-11-07T09:54:30Z"],["dc.date.issued","2015"],["dc.description.abstract","Clostridium sporogenes DSM795 is the type strain of the species Clostridium sporogenes, first described by Metchnikoff in 1908. It is a Gram-positive, rod-shaped, anaerobic bacterium isolated from human faeces and belongs to the proteolytic branch of clostridia. C. sporogenes attracts special interest because of its potential use in a bacterial therapy for certain cancer types. Genome sequencing and annotation revealed several gene clusters coding for proteins involved in anaerobic degradation of amino acids, such as glycine and betaine via Stickland reaction. Genome comparison showed that C. sporogenes is closely related to C. botulinum. The genome of C. sporogenes DSM 795 consists of a circular chromosome of 4.1 Mb with an overall GC content of 27.81 mol% harboring 3,744 protein-coding genes, and 80 RNAs."],["dc.identifier.doi","10.1186/s40793-015-0016-y"],["dc.identifier.isi","000367989900001"],["dc.identifier.pmid","26221421"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12507"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36550"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1944-3277"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","16"],["dc.bibliographiccitation.journal","Standards in Genomic Sciences"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Djukic, Marvin"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Strauss, Juliane"],["dc.contributor.author","Tann, Fabian Jannik"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Daniel, Rolf"],["dc.date.accessioned","2018-11-07T10:00:51Z"],["dc.date.available","2018-11-07T10:00:51Z"],["dc.date.issued","2015"],["dc.description.abstract","The lactic acid bacterium Lactobacillus kunkeei has been described as an inhabitant of fructose-rich niches. Here we report on the genome sequence of L. kunkeei EFB6, which has been isolated from a honeybee larva infected with European foulbrood. The draft genome comprises 1,566,851 bp and 1,417 predicted protein-encoding genes."],["dc.description.sponsorship","Open-Access Publikationsafonds 2015"],["dc.identifier.doi","10.1186/1944-3277-10-16"],["dc.identifier.isi","000367985600001"],["dc.identifier.pmid","26203329"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12523"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37894"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1944-3277"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","High quality draft genome of Lactobacillus kunkeei EFB6, isolated from a German European foulbrood outbreak of honeybees"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","359"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Vollmers, John"],["dc.contributor.author","Goerlich, Dennis"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Dobrindt, Ulrich"],["dc.date.accessioned","2018-11-07T10:23:54Z"],["dc.date.available","2018-11-07T10:23:54Z"],["dc.date.issued","2017"],["dc.description.abstract","Background: Escherichia coli bovine mastitis is a disease of significant economic importance in the dairy industry. Molecular characterization of mastitis-associated E. coli (MAEC) did not result in the identification of common traits. Nevertheless, a mammary pathogenic E. coli (MPEC) pathotype has been proposed suggesting virulence traits that differentiate MAEC from commensal E. coli. The present study was designed to investigate the MPEC pathotype hypothesis by comparing the genomes of MAEC and commensal bovine E. coli. Results: We sequenced the genomes of eight E. coli isolated from bovine mastitis cases and six fecal commensal isolates from udder-healthy cows. We analyzed the phylogenetic history of bovine E. coli genomes by supplementing this strain panel with eleven bovine-associated E. coli from public databases. The majority of the isolates originate from phylogroups A and B1, but neither MAEC nor commensal strains could be unambiguously distinguished by phylogenetic lineage. The gene content of both MAEC and commensal strains is highly diverse and dominated by their phylogenetic background. Although individual strains carry some typical E. coli virulence-associated genes, no traits important for pathogenicity could be specifically attributed to MAEC. Instead, both commensal strains and MAEC have very few gene families enriched in either pathotype. Only the aerobactin siderophore gene cluster was enriched in commensal E. coli within our strain panel. Conclusions: This is the first characterization of a phylogenetically diverse strain panel including several MAEC and commensal isolates. With our comparative genomics approach we could not confirm previous studies that argue for a positive selection of specific traits enabling MAEC to elicit bovine mastitis. Instead, MAEC are facultative and opportunistic pathogens recruited from the highly diverse bovine gastrointestinal microbiota. Virulence-associated genes implicated in mastitis are a by-product of commensalism with the primary function to enhance fitness in the bovine gastrointestinal tract. Therefore, we put the definition of the MPEC pathotype into question and suggest to designate corresponding isolates as MAEC."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [DO 789/3-1, DO 789/4-1]"],["dc.identifier.doi","10.1186/s12864-017-3739-x"],["dc.identifier.isi","000401575000003"],["dc.identifier.pmid","28482799"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14718"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42549"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","No evidence for a bovine mastitis Escherichia coli pathotype"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]