Poehlein, Anja

[["dc.bibliographiccitation.artnumber","16600"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Amiri Moghaddam, Jamshid"],["dc.contributor.author","Crüsemann, Max"],["dc.contributor.author","Alanjary, Mohammad"],["dc.contributor.author","Harms, Henrik"],["dc.contributor.author","Dávila-Céspedes, Antonio"],["dc.contributor.author","Blom, Jochen"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Ziemert, Nadine"],["dc.contributor.author","König, Gabriele M."],["dc.contributor.author","Schäberle, Till F."],["dc.date.accessioned","2019-07-09T11:51:03Z"],["dc.date.available","2019-07-09T11:51:03Z"],["dc.date.issued","2018"],["dc.description.abstract","Comparative genomic/metabolomic analysis is a powerful tool to disclose the potential of microbes for the biosynthesis of novel specialized metabolites. In the group of marine myxobacteria only a limited number of isolated species and sequenced genomes is so far available. However, the few compounds isolated thereof so far show interesting bioactivities and even novel chemical scaffolds; thereby indicating a huge potential for natural product discovery. In this study, all marine myxobacteria with accessible genome data (n = 5), including Haliangium ochraceum DSM 14365, Plesiocystis pacifica DSM 14875, Enhygromyxa salina DSM 15201 and the two newly sequenced species Enhygromyxa salina SWB005 and SWB007, were analyzed. All of these accessible genomes are large (~10 Mb), with a relatively small core genome and many unique coding sequences in each strain. Genome analysis revealed a high variety of biosynthetic gene clusters (BGCs) between the strains and several resistance models and essential core genes indicated the potential to biosynthesize antimicrobial molecules. Polyketides (PKs) and terpenes represented the majority of predicted specialized metabolite BGCs and contributed to the highest share between the strains. BGCs coding for non-ribosomal peptides (NRPs), PK/NRP hybrids and ribosomally synthesized and post-translationally modified peptides (RiPPs) were mostly strain specific. These results were in line with the metabolomic analysis, which revealed a high diversity of the chemical features between the strains. Only 6-11% of the metabolome was shared between all the investigated strains, which correlates to the small core genome of these bacteria (13-16% of each genome). In addition, the compound enhygrolide A, known from E. salina SWB005, was detected for the first time and structurally elucidated from Enhygromyxa salina SWB006. The here acquired data corroborate that these microorganisms represent a most promising source for the detection of novel specialized metabolites."],["dc.identifier.doi","10.1038/s41598-018-34954-y"],["dc.identifier.pmid","30413766"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16038"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59865"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Analysis of the Genome and Metabolome of Marine Myxobacteria Reveals High Potential for Biosynthesis of Novel Specialized Metabolites"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Spröer, Cathrin"],["dc.contributor.author","Wetzel, Daniela"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Chibani, Cynthia"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Liesegang, Heiko"],["dc.date.accessioned","2019-07-09T11:45:11Z"],["dc.date.available","2019-07-09T11:45:11Z"],["dc.date.issued","2018"],["dc.description.abstract","BACKGROUND: Clostridioides difficile infections (CDI) have emerged over the past decade causing symptoms that range from mild, antibiotic-associated diarrhea (AAD) to life-threatening toxic megacolon. In this study, we describe a multiple and isochronal (mixed) CDI caused by the isolates DSM 27638, DSM 27639 and DSM 27640 that already initially showed different morphotypes on solid media. RESULTS: The three isolates belonging to the ribotypes (RT) 012 (DSM 27639) and 027 (DSM 27638 and DSM 27640) were phenotypically characterized and high quality closed genome sequences were generated. The genomes were compared with seven reference strains including three strains of the RT 027, two of the RT 017, and one of the RT 078 as well as a multi-resistant RT 012 strain. The analysis of horizontal gene transfer events revealed gene acquisition incidents that sort the strains within the time line of the spread of their RTs within Germany. We could show as well that horizontal gene transfer between the members of different RTs occurred within this multiple infection. In addition, acquisition and exchange of virulence-related features including antibiotic resistance genes were observed. Analysis of the two genomes assigned to RT 027 revealed three single nucleotide polymorphisms (SNPs) and apparently a regional genome modification within the flagellar switch that regulates the fli operon. CONCLUSION: Our findings show that (i) evolutionary events based on horizontal gene transfer occur within an ongoing CDI and contribute to the adaptation of the species by the introduction of new genes into the genomes, (ii) within a multiple infection of a single patient the exchange of genetic material was responsible for a much higher genome variation than the observed SNPs."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2018"],["dc.identifier.doi","10.1186/s12864-017-4368-0"],["dc.identifier.pmid","29291715"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15054"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59178"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/15123 but duplicate"],["dc.notes.status","final"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Comparative genome and phenotypic analysis of three Clostridioides difficile strains isolated from a single patient provide insight into multiple infection of C. difficile."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","The ISME Journal"],["dc.contributor.author","Bischoff, Vera"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Meier-Kolthoff, Jan P."],["dc.contributor.author","Spröer, Cathrin"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Dogs, Marco"],["dc.contributor.author","Nguyen, Mary"],["dc.contributor.author","Petersen, Jörn"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Göker, Markus"],["dc.contributor.author","Simon, Meinhard"],["dc.contributor.author","Brinkhoff, Thorsten"],["dc.contributor.author","Moraru, Cristina"],["dc.date.accessioned","2019-07-09T11:51:06Z"],["dc.date.available","2019-07-09T11:51:06Z"],["dc.date.issued","2019"],["dc.description.abstract","Bacteriophages are widely considered to influence bacterial communities, however most phages are still unknown or not studied well enough to understand their ecological roles. We have isolated two phages infecting Lentibacter sp. SH36, affiliated with the marine Roseobacter group, and retrieved similar phage genomes from publicly available metagenomics databases. Phylogenetic analysis placed the new phages within the Cobavirus group, in the here newly proposed genus Siovirus and subfamily Riovirinae of the Podoviridae. Gene composition and presence of direct terminal repeats in cultivated cobaviruses point toward a genome replication and packaging strategy similar to the T7 phage. Investigation of the genomes suggests that viral lysis of the cell proceeds via the canonical holin-endolysin pathway. Cobaviral hosts include members of the genera Lentibacter, Sulfitobacter and Celeribacter of the Roseobacter group within the family Rhodobacteraceae (Alphaproteobacteria). Screening more than 5,000 marine metagenomes, we found cobaviruses worldwide from temperate to tropical waters, in the euphotic zone, mainly in bays and estuaries, but also in the open ocean. The presence of cobaviruses in protist metagenomes as well as the phylogenetic neighborhood of cobaviruses in glutaredoxin and ribonucleotide reductase trees suggest that cobaviruses could infect bacteria associated with phototrophic or grazing protists. With this study, we expand the understanding of the phylogeny, classification, genomic organization, biogeography and ecology of this phage group infecting marine Rhodobacteraceae."],["dc.identifier.doi","10.1038/s41396-019-0362-7"],["dc.identifier.pmid","30718806"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16049"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59876"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Cobaviruses – a new globally distributed phage group infecting Rhodobacteraceae in marine ecosystems"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","419"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Genes"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Djukic, Marvin"],["dc.contributor.author","Erler, Silvio"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Grossar, Daniela"],["dc.contributor.author","Charrière, Jean-Daniel"],["dc.contributor.author","Gauthier, Laurent"],["dc.contributor.author","Hartken, Denise"],["dc.contributor.author","Dietrich, Sascha"],["dc.contributor.author","Nacke, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Poehlein, Anja"],["dc.date.accessioned","2019-07-09T11:45:46Z"],["dc.date.available","2019-07-09T11:45:46Z"],["dc.date.issued","2018"],["dc.description.abstract","In Europe, approximately 84% of cultivated crop species depend on insect pollinators, mainly bees. Apis mellifera (the Western honey bee) is the most important commercial pollinator worldwide. The Gram-positive bacterium Melissococcus plutonius is the causative agent of European foulbrood (EFB), a global honey bee brood disease. In order to detect putative virulence factors, we sequenced and analyzed the genomes of 14 M. plutonius strains, including two reference isolates. The isolates do not show a high diversity in genome size or number of predicted protein-encoding genes, ranging from 2.021 to 2.101 Mbp and 1589 to 1686, respectively. Comparative genomics detected genes that might play a role in EFB pathogenesis and ultimately in the death of the honey bee larvae. These include bacteriocins, bacteria cell surface- and host cell adhesion-associated proteins, an enterococcal polysaccharide antigen, an epsilon toxin, proteolytic enzymes, and capsule-associated proteins. In vivo expression of three putative virulence factors (endo-alpha-N-acetylgalactosaminidase, enhancin and epsilon toxin) was verified using naturally infected larvae. With our strain collection, we show for the first time that genomic differences exist between non-virulent and virulent typical strains, as well as a highly virulent atypical strain, that may contribute to the virulence of M. plutonius. Finally, we also detected a high number of conserved pseudogenes (75 to 156) per genome, which indicates genomic reduction during evolutionary host adaptation."],["dc.identifier.doi","10.3390/genes9080419"],["dc.identifier.pmid","30127293"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15313"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59307"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2073-4425"],["dc.relation.issn","2073-4425"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Comparative Genomics and Description of Putative Virulence Factors of Melissococcus plutonius, the Causative Agent of European Foulbrood Disease in Honey Bees"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2241"],["dc.bibliographiccitation.journal","Frontiers in microbiology"],["dc.bibliographiccitation.lastpage","2241"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Davidsson, Sabina"],["dc.contributor.author","Carlsson, Jessica"],["dc.contributor.author","Mölling, Paula"],["dc.contributor.author","Gashi, Natyra"],["dc.contributor.author","Andrén, Ove"],["dc.contributor.author","Andersson, Swen-Olof"],["dc.contributor.author","Brzuszkiewicz, Elzbieta"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Al-Zeer, Munir A"],["dc.contributor.author","Brinkmann, Volker"],["dc.contributor.author","Scavenius, Carsten"],["dc.contributor.author","Nazipi, Seven"],["dc.contributor.author","Söderquist, Bo"],["dc.contributor.author","Brüggemann, Holger"],["dc.date.accessioned","2019-07-09T11:44:46Z"],["dc.date.available","2019-07-09T11:44:46Z"],["dc.date.issued","2017"],["dc.description.abstract","Inflammation is one of the hallmarks of prostate cancer. The origin of inflammation is unknown, but microbial infections are suspected to play a role. In previous studies, the Gram-positive, low virulent bacterium Cutibacterium (formerly Propionibacterium) acnes was frequently isolated from prostatic tissue. It is unclear if the presence of the bacterium represents a true infection or a contamination. Here we investigated Cutibacterium acnes type II, also called subspecies defendens, which is the most prevalent type among prostatic C. acnes isolates. Genome sequencing of type II isolates identified large plasmids in several genomes. The plasmids are highly similar to previously identified linear plasmids of type I C. acnes strains associated with acne vulgaris. A PCR-based analysis revealed that 28.4% (21 out of 74) of all type II strains isolated from cancerous prostates carry a plasmid. The plasmid shows signatures for conjugative transfer. In addition, it contains a gene locus for tight adherence (tad) that is predicted to encode adhesive Flp (fimbrial low-molecular weight protein) pili. In subsequent experiments a tad locus-encoded putative pilin subunit was identified in the surface-exposed protein fraction of plasmid-positive C. acnes type II strains by mass spectrometry, indicating that the tad locus is functional. Additional plasmid-encoded proteins were detected in the secreted protein fraction, including two signal peptide-harboring proteins; the corresponding genes are specific for type II C. acnes, thus lacking from plasmid-positive type I C. acnes strains. Further support for the presence of Flp pili in C. acnes type II was provided by electron microscopy, revealing cell appendages in tad locus-positive strains. Our study provides new insight in the most prevalent prostatic subspecies of C. acnes, subsp. defendens, and indicates the existence of Flp pili in plasmid-positive strains. Such pili may support colonization and persistent infection of human prostates by C. acnes."],["dc.identifier.doi","10.3389/fmicb.2017.02241"],["dc.identifier.pmid","29201018"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14897"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59092"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e00819-20"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","40"],["dc.bibliographiccitation.journal","Microbiology Resource Announcements"],["dc.bibliographiccitation.lastpage","3"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Winkler, Lucia"],["dc.contributor.author","Münker, Marc F."],["dc.contributor.author","Brunotte, Susanne"],["dc.contributor.author","Rohlmann, Lina"],["dc.contributor.author","Diez Alfageme, Alvaro"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Reitner, Joachim"],["dc.contributor.author","Nacke, Heiko"],["dc.date.accessioned","2020-10-08T11:07:42Z"],["dc.date.accessioned","2021-10-27T13:14:04Z"],["dc.date.available","2020-10-08T11:07:42Z"],["dc.date.available","2021-10-27T13:14:04Z"],["dc.date.issued","2020"],["dc.description.abstract","We sequenced the metagenome of an anoxygenic photosynthetic consortium originating from pond water and reconstructed four metagenome-assembled genomes. These genomes include Desulfocapsa, Paludibacter, Lamprocystis, and Rhodocyclaceae representatives and indicate the presence of genes for dissimilatory sulfate reduction and oxidation of reduced sulfur compounds."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.1128/MRA.00819-20"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17589"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91829"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","2576-098X"],["dc.relation.orgunit","Fakultät für Geowissenschaften und Geographie"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Metagenome-Assembled Genome Sequences from an Anoxygenic Photosynthetic Consortium Involved in Sulfur Cycling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]