Flügel, Alexander

[["dc.bibliographiccitation.firstpage","eabd5647"],["dc.bibliographiccitation.issue","675"],["dc.bibliographiccitation.journal","Science Signaling"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Roggenkamp, Hannes G."],["dc.contributor.author","Khansahib, Imrankhan"],["dc.contributor.author","Hernandez C., Lola C."],["dc.contributor.author","Zhang, Yunpeng"],["dc.contributor.author","Lodygin, Dmitri"],["dc.contributor.author","Krüger, Aileen"],["dc.contributor.author","Gu, Feng"],["dc.contributor.author","Möckl, Franziska"],["dc.contributor.author","Löhndorf, Anke"],["dc.contributor.author","Wolters, Valerie"],["dc.contributor.author","Woike, Daniel"],["dc.contributor.author","Rosche, Anette"],["dc.contributor.author","Bauche, Andreas"],["dc.contributor.author","Schetelig, Daniel"],["dc.contributor.author","Werner, René"],["dc.contributor.author","Schlüter, Hartmut"],["dc.contributor.author","Failla, Antonio V."],["dc.contributor.author","Meier, Chris"],["dc.contributor.author","Fliegert, Ralf"],["dc.contributor.author","Walseth, Timothy F."],["dc.contributor.author","Flügel, Alexander"],["dc.contributor.author","Diercks, Björn-Philipp"],["dc.contributor.author","Guse, Andreas H."],["dc.date.accessioned","2021-04-14T08:28:14Z"],["dc.date.available","2021-04-14T08:28:14Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1126/scisignal.abd5647"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82545"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1937-9145"],["dc.relation.issn","1945-0877"],["dc.title","HN1L/JPT2: A signaling protein that connects NAADP generation to Ca 2+ microdomain formation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","eaat0358"],["dc.bibliographiccitation.issue","561"],["dc.bibliographiccitation.journal","Science Signaling"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Diercks, Björn-Philipp"],["dc.contributor.author","Werner, René"],["dc.contributor.author","Weidemüller, Paula"],["dc.contributor.author","Czarniak, Frederik"],["dc.contributor.author","Hernandez, Lola"],["dc.contributor.author","Lehmann, Cari"],["dc.contributor.author","Rosche, Annette"],["dc.contributor.author","Krüger, Aileen"],["dc.contributor.author","Kaufmann, Ulrike"],["dc.contributor.author","Vaeth, Martin"],["dc.contributor.author","Failla, Antonio V."],["dc.contributor.author","Zobiak, Bernd"],["dc.contributor.author","Kandil, Farid I."],["dc.contributor.author","Schetelig, Daniel"],["dc.contributor.author","Ruthenbeck, Alexandra"],["dc.contributor.author","Meier, Chris"],["dc.contributor.author","Lodygin, Dmitri"],["dc.contributor.author","Flügel, Alexander"],["dc.contributor.author","Ren, Dejian"],["dc.contributor.author","Wolf, Insa M. A."],["dc.contributor.author","Feske, Stefan"],["dc.contributor.author","Guse, Andreas H."],["dc.date.accessioned","2020-12-10T18:36:46Z"],["dc.date.available","2020-12-10T18:36:46Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1126/scisignal.aat0358"],["dc.identifier.eissn","1937-9145"],["dc.identifier.issn","1945-0877"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76733"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","ORAI1, STIM1/2, and RYR1 shape subsecond Ca 2+ microdomains upon T cell activation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","ra102"],["dc.bibliographiccitation.issue","398"],["dc.bibliographiccitation.journal","Science Signaling"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Wolf, Insa M. A."],["dc.contributor.author","Diercks, Bjoern-Philipp"],["dc.contributor.author","Gattkowski, Ellen"],["dc.contributor.author","Czarniak, Frederik"],["dc.contributor.author","Kempski, Jan"],["dc.contributor.author","Werner, Rene"],["dc.contributor.author","Schetelig, Daniel"],["dc.contributor.author","Mittrücker, Hans-Willi"],["dc.contributor.author","Schumacher, Valea"],["dc.contributor.author","von Osten, Manuel"],["dc.contributor.author","Lodygin, Dimitri"],["dc.contributor.author","Flügel, Alexander"],["dc.contributor.author","Fliegert, Ralf"],["dc.contributor.author","Guse, Andreas H."],["dc.date.accessioned","2018-11-07T09:50:15Z"],["dc.date.available","2018-11-07T09:50:15Z"],["dc.date.issued","2015"],["dc.description.abstract","The activation of T cells is the fundamental on switch for the adaptive immune system. Ca2+ signaling is essential for T cell activation and starts as initial, short-lived, localized Ca2+ signals. The second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) forms rapidly upon T cell activation and stimulates early Ca2+ signaling. We developed a high-resolution imaging technique using multiple fluorescent Ca2+ indicator dyes to characterize these early signaling events and investigate the channels involved in NAADP-dependent Ca2+ signals. In the first seconds of activation of either primary murine T cells or human Jurkat cells with beads coated with an antibody against CD3, we detected Ca2+ signals with diameters close to the limit of detection and that were close to the activation site at the plasma membrane. In Jurkat cells in which the ryanodine receptor (RyR) was knocked down or in primary T cells from RyR1(-/-) mice, either these early Ca2+ signals were not detected or the number of signals was markedly reduced. Local Ca2+ signals observed within 20 ms upon microinjection of Jurkat cells with NAADP were also sensitive to RyR knockdown. In contrast, TRPM2 (transient receptor potential channel, subtype melastatin 2), a potential NAADP target channel, was not required for the formation of initial Ca2+ signals in primary T cells. Thus, through our high-resolution imaging method, we characterized early Ca2+ release events in T cells and obtained evidence for the involvement of RyR and NAADP in such signals."],["dc.identifier.doi","10.1126/scisignal.aab0863"],["dc.identifier.isi","000363319600002"],["dc.identifier.pmid","26462735"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35676"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1937-9145"],["dc.relation.issn","1945-0877"],["dc.title","Frontrunners of T cell activation: Initial, localized Ca2+ signals mediated by NAADP and the type 1 ryanodine receptor"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","139"],["dc.bibliographiccitation.journal","Biochemical Journal"],["dc.bibliographiccitation.lastpage","149"],["dc.bibliographiccitation.volume","422"],["dc.contributor.author","Kirchberger, Tanja"],["dc.contributor.author","Moreau, Christelle"],["dc.contributor.author","Wagner, Gerd K."],["dc.contributor.author","Fliegert, Ralf"],["dc.contributor.author","Siebrands, Cornelia C."],["dc.contributor.author","Nebel, Merle"],["dc.contributor.author","Schmid, Frederike"],["dc.contributor.author","Harneit, Angelika"],["dc.contributor.author","Odoardi, Francesca"],["dc.contributor.author","Fluegel, Alexander"],["dc.contributor.author","Potter, Barry V. L."],["dc.contributor.author","Guse, Andreas H."],["dc.date.accessioned","2018-11-07T11:25:42Z"],["dc.date.available","2018-11-07T11:25:42Z"],["dc.date.issued","2009"],["dc.description.abstract","cADPR (cyclic ADP-ribose) is a universal Ca(2+) mobilizing second messenger. In T-cells cADPR is involved in sustained Ca(2+) release and also in Ca(2+) entry. Potential mechanisms for the latter include either capacitative Ca(2+) entry, secondary to store depletion by cADPR, or direct activation of the non-selective cation channel TRPM2 (transient receptor potential cation channel, subfamily melastatin, member 2). Here we characterize the molecular target of the newly-described membrane-permeant cADPR agonist 8-Br-N(1)-cIDPR (8-bromo-cyclic IDP-ribose). 8-Br-N(1)-cIDPR evoked Ca(2+) signalling in the human T-lymphoma cell line Jurkat and in primary rat T-lymphocytes. Ca(2+) signalling induced by 8-Br-N(1)-cIDPR consisted of Ca(2+) release and Ca(2+) entry. Whereas Ca(2+) release was sensitive to both the RyR (ryanodine receptor) blocker RuRed (Ruthenium Red) and the cADPR antagonist 8-Br-cADPR (8-bromo-cyclic ADP-ribose), Ca(2+) entry was inhibited by the Ca(2+) entry blockers Gd(3+) (gadolinium ion) and SKF-96365, as well as by 8-Br-cADPR. To unravel a potential role for TRPM2 in Sustained Ca(2+) entry evoked by 8-Br-N(1)-cIDPR, TRPM2 was overexpressed in HEK (human embryonic kidney)293 cells. However, though activation by H(2)O(2) was enhanced dramatically in those cells, Ca(2+) Signalling induced by 8-Br-N(1)-cIDPR was almost unaffected. Similarly, direct analysis of TRPM2 currents did not reveal activation or co-activation of TRPM2 by 8-Br-N(1)-cIDPR. In summary, the sensitivity to the Ca(2+) entry blockers Gd(3+) and SKF-96365 is in favour of the concept of capacitative Ca(2+) entry, secondary to store depletion by 8-Br-N(1)-cIDPR. Taken together, 8-Br-N(1)-cIDPR appears to be the first cADPR agonist affecting Ca(2+) release and secondary Ca(2+) entry, but without effect on TRPM2."],["dc.identifier.doi","10.1042/BJ20082308"],["dc.identifier.isi","000269023100015"],["dc.identifier.pmid","19492987"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56686"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Portland Press Ltd"],["dc.relation.issn","0264-6021"],["dc.title","8-Bromo-cyclic inosine diphosphoribose: towards a selective cyclic ADP-ribose agonist"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]