Opitz, Lennart

[["dc.bibliographiccitation.journal","Wiener klinische Wochenschrift"],["dc.bibliographiccitation.volume","120"],["dc.contributor.author","Novota, P."],["dc.contributor.author","Sviland, Lisbet"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Hitt, Reiner"],["dc.contributor.author","Dickinson, Anne M."],["dc.contributor.author","Walter, L."],["dc.contributor.author","Dressel, Ralf"],["dc.date.accessioned","2018-11-07T11:12:51Z"],["dc.date.available","2018-11-07T11:12:51Z"],["dc.date.issued","2008"],["dc.format.extent","124"],["dc.identifier.isi","000259367100404"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53754"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Wien"],["dc.relation.issn","0043-5325"],["dc.title","Major histocompatibility complex (MHC) gene expression profiling of the graft versus host reaction in skin explant assays"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e16582"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Novota, Peter"],["dc.contributor.author","Zinocker, Severin"],["dc.contributor.author","Norden, Jean"],["dc.contributor.author","Wang, X."],["dc.contributor.author","Sviland, Lisbet"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Rolstad, Bent"],["dc.contributor.author","Dickinson, Anne M."],["dc.contributor.author","Walter, Lutz"],["dc.contributor.author","Dressel, Ralf"],["dc.date.accessioned","2018-11-07T09:00:02Z"],["dc.date.available","2018-11-07T09:00:02Z"],["dc.date.issued","2011"],["dc.description.abstract","Background: The major histocompatibility complex (MHC) is the most important genomic region that contributes to the risk of graft versus host disease (GVHD) after haematopoietic stem cell transplantation. Matching of MHC class I and II genes is essential for the success of transplantation. However, the MHC contains additional genes that also contribute to the risk of developing acute GVHD. It is difficult to identify these genes by genetic association studies alone due to linkage disequilibrium in this region. Therefore, we aimed to identify MHC genes and other genes involved in the pathophysiology of GVHD by mRNA expression profiling. Methodology/Principal Findings: To reduce the complexity of the task, we used genetically well-defined rat inbred strains and a rat skin explant assay, an in-vitro-model of the graft versus host reaction (GVHR), to analyze the expression of MHC, natural killer complex (NKC), and other genes in cutaneous GVHR. We observed a statistically significant and strong up or down regulation of 11 MHC, 6 NKC, and 168 genes encoded in other genomic regions, i.e. 4.9%, 14.0%, and 2.6% of the tested genes respectively. The regulation of 7 selected MHC and 3 NKC genes was confirmed by quantitative real-time PCR and in independent skin explant assays. In addition, similar regulations of most of the selected genes were observed in GVHD-affected skin lesions of transplanted rats and in human skin explant assays. Conclusions/Significance: We identified rat and human MHC and NKC genes that are regulated during GVHR in skin explant assays and could therefore serve as biomarkers for GVHD. Several of the respective human genes, including HLA-DMB, C2, AIF1, SPR1, UBD, and OLR1, are polymorphic. These candidates may therefore contribute to the genetic risk of GVHD in patients."],["dc.identifier.doi","10.1371/journal.pone.0016582"],["dc.identifier.isi","000286664100046"],["dc.identifier.pmid","21305040"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8196"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24049"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Expression Profiling of Major Histocompatibility and Natural Killer Complex Genes Reveals Candidates for Controlling Risk of Graft versus Host Disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Tissue Antigens"],["dc.bibliographiccitation.volume","77"],["dc.contributor.author","Novota, P."],["dc.contributor.author","Zinocker, Severin"],["dc.contributor.author","Norden, J."],["dc.contributor.author","Wang, X. N."],["dc.contributor.author","Sviland, Lisbet"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Rolstad, Bent"],["dc.contributor.author","Dickinson, Anne M."],["dc.contributor.author","Walter, L."],["dc.contributor.author","Dressel, Ralf"],["dc.date.accessioned","2018-11-07T08:56:48Z"],["dc.date.available","2018-11-07T08:56:48Z"],["dc.date.issued","2011"],["dc.format.extent","381"],["dc.identifier.isi","000289249000030"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23237"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Malden"],["dc.relation.conference","25th Conference on European Immunogenetics and Histocompatibility"],["dc.relation.eventlocation","Prague, CZECH REPUBLIC"],["dc.relation.issn","0001-2815"],["dc.title","Expression profiling of major histocompatibility and natural killer complex genes reveals new candidates for controlling risk of graft versus host disease"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e48869"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Khromov, Tatjana"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Siamishi, Iliana"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Pantakani, Dasaradha Venkata Krishna"],["dc.date.accessioned","2018-11-07T09:03:34Z"],["dc.date.available","2018-11-07T09:03:34Z"],["dc.date.issued","2012"],["dc.description.abstract","Stem cells in the developing embryo proliferate and differentiate while maintaining genomic integrity, failure of which may lead to accumulation of mutations and subsequent damage to the embryo. Embryonic stem cells (ESCs), the in vitro counterpart of embryo stem cells are highly sensitive to genotoxic stress. Defective ESCs undergo either efficient DNA damage repair or apoptosis, thus maintaining genomic integrity. However, the genotoxicity- and apoptosis-related processes in germ-line derived pluripotent cells, multipotent adult germ-line stem cells (maGSCs), are currently unknown. Here, we analyzed the expression of apoptosis-related genes using OligoGEArray in undifferentiated maGSCs and ESCs and identified a similar set of genes expressed in both cell types. We detected the expression of intrinsic, but not extrinsic, apoptotic pathway genes in both cell types. Further, we found that apoptosis-related gene expression patterns of differentiated ESCs and maGSCs are identical to each other. Comparative analysis revealed that several pro-and antiapoptotic genes are expressed specifically in pluripotent cells, but markedly downregulated in the differentiated counterparts of these cells. Activation of the intrinsic apoptotic pathway cause approximately similar to 35% of both ESCs and maGSCs to adopt an early-apoptotic phenotype. Moreover, we performed transcriptome studies using early-apoptotic cells to identify novel pluripotency- and apoptosis-related genes. From these transcriptome studies, we selected Fgf4 (Fibroblast growth factor 4) and Mnda (Myeloid cell nuclear differentiating antigen), which are highly downregulated in early-apoptotic cells, as novel candidates and analyzed their roles in apoptosis and genotoxicity responses in ESCs. Collectively, our results show the existence of common molecular mechanisms for maintaining the pristine stem cell pool of both ESCs and maGSCs."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.identifier.doi","10.1371/journal.pone.0048869"],["dc.identifier.isi","000311935800158"],["dc.identifier.pmid","23145002"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8319"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24921"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Apoptosis-Related Gene Expression Profiles of Mouse ESCs and maGSCs: Role of Fgf4 and Mnda in Pluripotent Cell Responses to Genotoxicity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]