Junek, Stephan

[["dc.bibliographiccitation.firstpage","1614"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Glia"],["dc.bibliographiccitation.lastpage","1624"],["dc.bibliographiccitation.volume","56"],["dc.contributor.author","Hassenklover, Thomas"],["dc.contributor.author","Kurtanska, Silvia"],["dc.contributor.author","Bartoszek, Ilonka"],["dc.contributor.author","Junek, Stephan"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T11:09:09Z"],["dc.date.available","2018-11-07T11:09:09Z"],["dc.date.issued","2008"],["dc.description.abstract","Extracellular purines and pyrimidines are important Signaling molecules acting via purinergic cell-surface receptors in neurons, glia, and glia-like cells such as sustentacular supporting cells (SCs) of the olfactory epithelium (OE). Here, we thoroughly characterize ATP-induced responses in SCs of the OE using functional Ca(2+) image The initial ATP-induced increase of the intracellular Ca(2+) concentration [Ca(2+)](i) always occurred in the apical part of SCs and subsequently propagated toward the basal lamina, indicating the occurrence of purinergic receptors I the apical part of SCs. The mean propagation velocity of the Ca(2+) signal within SCs was 17.10 +/- 1.02 mu m/s. ATP evoked increases in [Ca(2+)](i) in both the presence and absence of extracellular Ca(2+). Depletion of the intracellular Ca(2+) stores abolished the responses. This shows that the ATP-induced [Ca(2+)](i) increases were in large part, if not entirely, due to the activation of G protein-coupled receptors followed by Ca(2+) mobilization from intracellular stores, suggesting an involvement of P2Y receptors. The order of potency of the applied purinergic agonists was UTP > ATP > ATP-gamma S (with all others being only weakly active or inactive). The ATP-induced [Ca(2+)](i) increases could be reduced by the purinergic antagonists PPADS and RB2, but not by suramin. Our findings suggest that extracellular nucleotides in the OE activate SCs via P2Y(2)/P2Y(4)-like receptors and initiate a characteristic intraepithelial Ca(2+) wave. (C) 2008 Wiley-Liss, Inc."],["dc.description.sponsorship","DFG Research Center"],["dc.identifier.doi","10.1002/glia.20714"],["dc.identifier.isi","000260918100002"],["dc.identifier.pmid","18551628"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7757"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52943"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0894-1491"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Nucleotide-Induced Ca(2+) Signaling in Sustentacular Supporting Cells of the Olfactory Epithelium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]