Busse, Ann-Christin

[["dc.bibliographiccitation.firstpage","420"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Cytometry Part A"],["dc.bibliographiccitation.lastpage","428"],["dc.bibliographiccitation.volume","77A"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Hoffschulte, B."],["dc.contributor.author","Mueller, Georg Anton"],["dc.contributor.author","Tarnok, A."],["dc.contributor.author","Mittag, A."],["dc.date.accessioned","2018-11-07T08:43:29Z"],["dc.date.available","2018-11-07T08:43:29Z"],["dc.date.issued","2010"],["dc.description.abstract","In recent years, slide-based cytometry has become a key technology for polychromatic cytometric investigations, and many efforts have been made to increase the number of measurable fluorochromes for multiparametric analysis. Sequential photobleaching of fluorochromes next to very photostable dyes is one approach for this technology. As the ALEXA dyes are known to be photostable as compared to the conventional fluorochromes FITC, PE (Riggs et al., Am J Pathol 1958;34:1081-1097), and APC, a differentiation within a fluorochrome pair is possible. Here, we have analyzed the newly available NorthernLights (TM) secondary antibodies for use in slide-based cytometry and microscopy. Currently, these fluorochrome-conjugates are now available with three distinct excitation- and emission maxima (NL493, NL557, NL637). Their spectral properties are similar to the frequently used fluorochromes FITC, PE, and APC and can, therefore, be used with most common excitation sources of cytometers or microscopes. As the NorthernLights (TM) are bright, resistant to photobleaching, stable in alcohols and xylene and of affordable price, these dyes are promising candidates for use with most laser- and HBO/XBA-based fluorescence microscopy-like techniques. (C) 2010 International Society for Advancement of Cytometry"],["dc.identifier.doi","10.1002/cyto.a.20863"],["dc.identifier.isi","000277174000003"],["dc.identifier.pmid","20151452"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19976"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.publisher.place","Hoboken"],["dc.relation.conference","25th Congress of the International-Society-for-Advancement-of-Cytometry"],["dc.relation.eventlocation","Seattle, WA"],["dc.relation.issn","1552-4922"],["dc.title","NorthernLights in Slide-Based Cytometry and Microscopy"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","750"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Cytometry Part A"],["dc.bibliographiccitation.lastpage","751"],["dc.bibliographiccitation.volume","71A"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Tepper-Wessels, Kathrin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Hoffschulte, B."],["dc.contributor.author","Grabbe, Eckhard"],["dc.contributor.author","Mueller, Georg Anton"],["dc.date.accessioned","2018-11-07T10:58:51Z"],["dc.date.available","2018-11-07T10:58:51Z"],["dc.date.issued","2007"],["dc.identifier.isi","000249187800036"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50561"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.publisher.place","Hoboken"],["dc.relation.conference","17th Annual Meeting of the German-Society-of-Cytometry (DGfZ)"],["dc.relation.eventlocation","Univ Hosp, Regensburg, Regensburg, GERMANY"],["dc.relation.issn","1552-4922"],["dc.title","Molecular imaging in vivo - A comparative overview"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Nephrology Dialysis Transplantation"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Wessels, Johannes"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Hoffman, Robert M."],["dc.contributor.author","Mueller, Gerhard-Anton"],["dc.date.accessioned","2018-11-07T11:07:09Z"],["dc.date.available","2018-11-07T11:07:09Z"],["dc.date.issued","2007"],["dc.format.extent","233"],["dc.identifier.isi","000253320600644"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52485"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","44th ERA-EDTA Congress"],["dc.relation.eventlocation","Barcelona, SPAIN"],["dc.relation.issn","0931-0509"],["dc.title","Real-time subcellular imaging of renal cell carcinoma-cell trafficking in live mice"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","123"],["dc.bibliographiccitation.journal","Onkologie"],["dc.bibliographiccitation.lastpage","124"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Laffers, Wiebke"],["dc.contributor.author","Busse, A.-C."],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Gerstner, Andreas O. H."],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.date.accessioned","2018-11-07T08:46:30Z"],["dc.date.available","2018-11-07T08:46:30Z"],["dc.date.issued","2010"],["dc.identifier.isi","000274745200344"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20707"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.publisher.place","Basel"],["dc.title","Photodynamic effects of hypericin on head and neck carcinoma in vitro and in vivo"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","711"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","European Archives of Oto-Rhino-Laryngology"],["dc.bibliographiccitation.lastpage","718"],["dc.bibliographiccitation.volume","272"],["dc.contributor.author","Laffers, Wiebke"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Phuc Nguyen, Phuc Nguyen"],["dc.contributor.author","Gerstner, Andreas O. H."],["dc.contributor.author","Bootz, Friedrich"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.date.accessioned","2018-11-07T10:00:38Z"],["dc.date.available","2018-11-07T10:00:38Z"],["dc.date.issued","2015"],["dc.description.abstract","Clinical outcome of patients suffering from head neck squamous cell carcinomas is still poor due to recurrent disease and surgical limitations. There is still a demand for multimodality approaches and new therapeutic options. Hypericin is a promising phototoxic drug which was investigated for its effects on head neck squamous cell carcinoma cells in vitro. FaDu cells incubated with or without hypericin were illuminated (450-700 nm, 50,000 lx) for different time periods. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide- and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay were used to score metabolic and apoptotic activity. Even after the shortest illumination FaDu cells incubated with hypericin showed massive reduction of metabolism and excessive apoptosis. This was present even with the lowest hypericin concentration. Cells without hypericin or without illumination were not affected. These photosensitizing effects of hypericin could be suitable for clinical application and could lead to the development of an intraoperative photodynamic therapy of head neck squamous cell carcinomas."],["dc.identifier.doi","10.1007/s00405-014-2984-8"],["dc.identifier.isi","000350031400027"],["dc.identifier.pmid","24687800"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37852"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1434-4726"],["dc.relation.issn","0937-4477"],["dc.title","Photosensitizing effects of hypericin on head neck squamous cell carcinoma in vitro"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","542"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Cytometry Part A"],["dc.bibliographiccitation.lastpage","549"],["dc.bibliographiccitation.volume","71A"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Grabbe, Eckhard"],["dc.contributor.author","Mueller, Georg Anton"],["dc.date.accessioned","2018-11-07T10:59:50Z"],["dc.date.available","2018-11-07T10:59:50Z"],["dc.date.issued","2007"],["dc.description.abstract","The multiparametric molecular cell and tissue analysis in vitro and in vivo is characterized by rapid progress in the field of image generation technologies, sensor biotechnology, and computational modeling. Fascinating new potentials in unraveling the detailed functions of single cells, organs, and whole organisms are presently emerging and permit the close monitoring i.e. tumor development or basic cell development processes with an unprecedented multiplicity of promising investigative possibilities. To answer basic questions of in vivo tumor development and progression fluorescence based imaging techniques provide new insights into molecular pathways and targets. Genetic reporter systems (eGFP, DsRED) are available and high sensitive detection systems are on hand. These techniques could be used for in vitro assays and quantified e.g. by microscopy and CCD based readouts. The introduction of novel fluorescent dyes emitting in the near infrared range (NIR) combined with the development of sensitive detector Systems and monochromatic powerful NIR-lasers for the first time permits the quantification and imaging of fluorescence and/or bioluminescence in deeper tissues. Laser based techniques particularly in the NIR-range (like two-photon microscopy) offer superb signal to noise ratios, and thus the potential to detect molecular targets in vivo. In combination with flat panel volumetric computed tomography (fpVCT), questions dealing e.g. with tumor size, tumor growth, and angiogenesis/vascularization could be answered noninvasively using the same animal. The resolution of down to 150 mu m/each direction can be achieved using fpVCT. It is demonstrated by many groups that submillimeter resolutions can be achieved in small animal imaging at high sensitivity and molecular specificity. Since the resolution in preclinical small animal imaging is down to similar to 10 pm by the use of microCT and to subcellular resolutions using (similar to 1 mu m) microscope based systems, the advances of different techniques can now be combined to \"multimodal\" preclinical imaging and the possibilities for in vivo intravital cytometry now become within one's reach. (C) 2007 International Society for Analytical Cytology"],["dc.identifier.doi","10.1002/cyto.a.20419"],["dc.identifier.isi","000248434300005"],["dc.identifier.pmid","17598185"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50789"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1552-4922"],["dc.title","In vivo imaging in experimental preclinical tumor research - A review"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Cytometry Part A"],["dc.bibliographiccitation.volume","71A"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Seitz, G."],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Hoffschult, B."],["dc.contributor.author","Mueller, Georg Anton"],["dc.date.accessioned","2018-11-07T10:58:50Z"],["dc.date.available","2018-11-07T10:58:50Z"],["dc.date.issued","2007"],["dc.format.extent","745"],["dc.identifier.isi","000249187800024"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50558"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.publisher.place","Hoboken"],["dc.relation.conference","17th Annual Meeting of the German-Society-of-Cytometry (DGfZ)"],["dc.relation.eventlocation","Univ Hosp, Regensburg, Regensburg, GERMANY"],["dc.relation.issn","1552-4922"],["dc.title","Visualizing cell traffic of single tumor cells live and in vivo by a new intravital microscopy approach"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","47"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cytometry Part A"],["dc.bibliographiccitation.lastpage","48"],["dc.bibliographiccitation.volume","71A"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Mahrt, Jens"],["dc.contributor.author","Busse, Ann-Christin"],["dc.contributor.author","Zaenker, Stephan"],["dc.contributor.author","Grabbe, Eckhard"],["dc.contributor.author","Mueller, Georg Anton"],["dc.date.accessioned","2018-11-07T11:06:23Z"],["dc.date.available","2018-11-07T11:06:23Z"],["dc.date.issued","2007"],["dc.identifier.isi","000243900300011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52297"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.publisher.place","Hoboken"],["dc.relation.conference","11th Leipziger Workshop/ 4th International Workshop on Slide Based Cytometry"],["dc.relation.eventlocation","Leipzig, GERMANY"],["dc.relation.issn","1552-4922"],["dc.title","New methods in optical and molecular tumour imaging - An update"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]