Westphal, Volker

[["dc.bibliographiccitation.firstpage","S123.2"],["dc.bibliographiccitation.issue","suppl 1"],["dc.bibliographiccitation.journal","Cardiovascular Research"],["dc.bibliographiccitation.lastpage","S123"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Hebisch, Elke"],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2017-09-07T11:53:09Z"],["dc.date.available","2017-09-07T11:53:09Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.1093/cvr/cvu098.101"],["dc.identifier.gro","3145042"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2734"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","Oxford University Press (OUP)"],["dc.relation.issn","0008-6363"],["dc.title","P677Superresolution microscopy reveals proliferative T-Tubule remodeling as general disease mechanism in early stages of heart failure development"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","305a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Lauterbach, Marcel"],["dc.contributor.author","Minh Tuan, Hoang-Trong"],["dc.contributor.author","Williams, George S.B."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Jafri, M. Saleet"],["dc.contributor.author","Lederer, W.J."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2022-03-01T11:44:54Z"],["dc.date.available","2022-03-01T11:44:54Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1016/j.bpj.2011.11.1683"],["dc.identifier.pii","S0006349511030311"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103157"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","STED Nanoscopy of Cardiac RyR2 Clusters and Sub-Structure Analysis After Myocardial Infarction"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The Journal of General Physiology"],["dc.bibliographiccitation.volume","136"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Wagner, Edward J."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hagen, Brian M."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T08:41:47Z"],["dc.date.available","2018-11-07T08:41:47Z"],["dc.date.issued","2010"],["dc.format.extent","9A"],["dc.identifier.isi","000279473500028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19548"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Rockefeller Univ Press"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Marine Biol Lab, Woods Hole, MA"],["dc.relation.issn","0022-1295"],["dc.title","Super-Resolution Imaging of Cardiac Signaling Microdomains by STED Microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","223A"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","224A"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Wagner, Eva M."],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Streich, Jan-Hendrik"],["dc.contributor.author","Tuan, Hoang-Trong M."],["dc.contributor.author","Hagen, Brian M."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Jafri, Mohsin S."],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T09:14:13Z"],["dc.date.available","2018-11-07T09:14:13Z"],["dc.date.issued","2012"],["dc.identifier.isi","000321561201431"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27356"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","0006-3495"],["dc.title","Live Cell Super-Resolution Imaging of Transverse Membrane Tubules in Heart Failure"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","402"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","414"],["dc.bibliographiccitation.volume","111"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Streich, Jan-Hendrik"],["dc.contributor.author","Korff, Brigitte"],["dc.contributor.author","Tuan, Hoang-Trong M."],["dc.contributor.author","Hagen, Brian"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Jafri, M. Saleet"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2017-09-07T11:48:29Z"],["dc.date.available","2017-09-07T11:48:29Z"],["dc.date.issued","2012"],["dc.description.abstract","Rationale: Transverse tubules (TTs) couple electric surface signals to remote intracellular Ca2+ release units (CRUs). Diffraction-limited imaging studies have proposed loss of TT components as disease mechanism in heart failure (HF). Objectives: Objectives were to develop quantitative super-resolution strategies for live-cell imaging of TT membranes in intact cardiomyocytes and to show that TT structures are progressively remodeled during HF development, causing early CRU dysfunction. Methods and Results: Using stimulated emission depletion (STED) microscopy, we characterized individual TTs with nanometric resolution as direct readout of local membrane morphology 4 and 8 weeks after myocardial infarction (4pMI and 8pMI). Both individual and network TT properties were investigated by quantitative image analysis. The mean area of TT cross sections increased progressively from 4pMI to 8pMI. Unexpectedly, intact TT networks showed differential changes. Longitudinal and oblique TTs were significantly increased at 4pMI, whereas transversal components appeared decreased. Expression of TT-associated proteins junctophilin-2 and caveolin-3 was significantly changed, correlating with network component remodeling. Computational modeling of spatial changes in HF through heterogeneous TT reorganization and RyR2 orphaning (5000 of 20 000 CRUs) uncovered a local mechanism of delayed subcellular Ca2+ release and action potential prolongation. Conclusions: This study introduces STED nanoscopy for live mapping of TT membrane structures. During early HF development, the local TT morphology and associated proteins were significantly altered, leading to differential network remodeling and Ca2+ release dyssynchrony. Our data suggest that TT remodeling during HF development involves proliferative membrane changes, early excitation-contraction uncoupling, and network fracturing."],["dc.identifier.doi","10.1161/CIRCRESAHA.112.274530"],["dc.identifier.gro","3142487"],["dc.identifier.isi","000307308700007"],["dc.identifier.pmid","22723297"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8829"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/73"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A05: Molekulares Imaging von kardialen Calcium-Freisetzungsdomänen"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.issn","0009-7330"],["dc.relation.workinggroup","RG Hasenfuß (Transition zur Herzinsuffizienz)"],["dc.relation.workinggroup","RG Hell"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.title","Stimulated Emission Depletion Live-Cell Super-Resolution Imaging Shows Proliferative Remodeling of T-Tubule Membrane Structures After Myocardial Infarction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","13"],["dc.bibliographiccitation.journal","Journal of Molecular and Cellular Cardiology"],["dc.bibliographiccitation.lastpage","21"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2017-09-07T11:47:42Z"],["dc.date.accessioned","2019-02-27T09:31:09Z"],["dc.date.available","2017-09-07T11:47:42Z"],["dc.date.available","2019-02-27T09:31:09Z"],["dc.date.issued","2013"],["dc.description.abstract","Detailed understanding of the adaptive nature of cardiac cells in health and disease requires investigation of proteins and membranes in their native physiological environment, ideally by noninvasive optical methods. However, conventional light microscopy does not resolve the spatial characteristics of small fluorescently labeled protein or membrane structures in cells. Due to diffraction limiting resolution to half the wavelength of light, adjacent fluorescent molecules spaced at less than ~250 nm are not separately visualized. This fundamental problem has lead to a rapidly growing area of research, superresolution fluorescence microscopy, also called nanoscopy. We discuss pioneering applications of superresolution microscopy relevant to the heart, emphasizing different nanoscopy strategies toward new insight in cardiac cell biology. Here, we focus on molecular and structural readouts from subcellular nanodomains and organelles related to Ca(2+) signaling during excitation-contraction (EC) coupling, including live cell imaging strategies. Based on existing data and superresolution techniques, we suggest that an important future aim will be subcellular in situ structure-function analysis with nanometric resolving power in organotypic cells."],["dc.identifier.doi","10.1016/j.yjmcc.2012.11.016"],["dc.identifier.gro","3142353"],["dc.identifier.isi","000317997600004"],["dc.identifier.pmid","23219451"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8981"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57633"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7342"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/27"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A05: Molekulares Imaging von kardialen Calcium-Freisetzungsdomänen"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.eissn","1095-8584"],["dc.relation.issn","0022-2828"],["dc.relation.issn","1095-8584"],["dc.relation.workinggroup","RG Hell"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Superresolution microscopy in heart - Cardiac nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","overview_ja"],["dc.type.version","submitted_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Hoang-Trong Minh Tuan, Hoang-Trong Minh Tuan"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Jafri, Mohsin S."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T09:14:13Z"],["dc.date.available","2018-11-07T09:14:13Z"],["dc.date.issued","2012"],["dc.format.extent","305A"],["dc.identifier.isi","000321561202133"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27357"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","0006-3495"],["dc.title","STED Nanoscopy of Cardiac RyR2 Clusters and Sub-Structure Analysis After Myocardial Infarction"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Wagner, Eva M."],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hagen, Brian M."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T08:47:09Z"],["dc.date.available","2018-11-07T08:47:09Z"],["dc.date.issued","2010"],["dc.format.extent","5A"],["dc.identifier.isi","000208762000029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20875"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.issn","0006-3495"],["dc.title","Sted Based Super-Resolution Imaging of Transverse Tubules in Ventricular Myocytes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","suppl 1"],["dc.bibliographiccitation.journal","European Heart Journal"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Jafri, Mohsin S."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2018-11-07T09:21:32Z"],["dc.date.available","2018-11-07T09:21:32Z"],["dc.date.issued","2013"],["dc.format.extent","7"],["dc.identifier.doi","10.1093/eurheartj/eht307.34"],["dc.identifier.gro","3145040"],["dc.identifier.isi","000327744600016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29129"],["dc.notes.intern","Crossref Import"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.eventlocation","Amsterdam, NETHERLANDS"],["dc.relation.issn","1522-9645"],["dc.relation.issn","0195-668X"],["dc.title","Proliferative membrane changes of T-tubules and cell-wide network changes during cardiac remodeling with orphaning of Ca2+release sites are a general mechanism of heart failure"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Lauterbach, Marcel"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hagen, Brian"],["dc.contributor.author","Lederer, W.J."],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2022-03-01T11:44:53Z"],["dc.date.available","2022-03-01T11:44:53Z"],["dc.date.issued","2010"],["dc.identifier.doi","10.1016/j.bpj.2009.12.034"],["dc.identifier.pii","S0006349509018396"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103149"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","Sted Based Super-Resolution Imaging of Transverse Tubules in Ventricular Myocytes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]