Westphal, Volker

[["dc.bibliographiccitation.firstpage","246"],["dc.bibliographiccitation.issue","5873"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.lastpage","249"],["dc.bibliographiccitation.volume","320"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:45Z"],["dc.date.available","2017-09-07T11:48:45Z"],["dc.date.issued","2008"],["dc.description.abstract","We present video-rate (28 frames per second) far-field optical imaging with a focal spot size of 62 nanometers in living cells. Fluorescently labeled synaptic vesicles inside the axons of cultured neurons were recorded with stimulated emission depletion (STED) microscopy in a 2.5-micrometer by 1.8-micrometer field of view. By reducing the cross-sectional area of the focal spot by about a factor of 18 below the diffraction limit (260 nanometers), STED allowed us to map and describe the vesicle mobility within the highly confined space of synaptic boutons. Although restricted within boutons, the vesicle movement was substantially faster in nonbouton areas, consistent with the observation that a sizable vesicle pool continuously transits through the axons. Our study demonstrates the emerging ability of optical microscopy to investigate intracellular physiological processes on the nanoscale in real time."],["dc.identifier.doi","10.1126/science.1154228"],["dc.identifier.gro","3143316"],["dc.identifier.isi","000254836700048"],["dc.identifier.pmid","18292304"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/817"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0036-8075"],["dc.title","Video-rate far-field optical nanoscopy dissects synaptic vesicle movement"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","675"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","684"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Rizzoli, Silvio"],["dc.date.accessioned","2017-09-07T11:45:20Z"],["dc.date.available","2017-09-07T11:45:20Z"],["dc.date.issued","2010"],["dc.description.abstract","Synaptic vesicles need to be mobile to reach their release sites during synaptic activity. We investigated vesicle mobility throughout the synaptic vesicle cycle using both conventional and subdiffraction-resolution stimulated emission depletion fluorescence microscopy. Vesicle tracking revealed that recently endocytosed synaptic vesicles are highly mobile for a substantial time period after endocytosis. They later undergo a maturation process and integrate into vesicle clusters where they exhibit little mobility. Despite the differences in mobility, both recently endocytosed and mature vesicles are exchanged between synapses. Electrical stimulation does not seem to affect the mobility of the two types of vesicles. After exocytosis, the vesicle material is mobile in the plasma membrane, although the movement appears to be somewhat limited. Increasing the proportion of fused vesicles (by stimulating exocytosis while simultaneously blocking endocytosis) leads to substantially higher mobility. We conclude that both high- and low-mobility states are characteristic of synaptic vesicle movement."],["dc.identifier.doi","10.1016/j.bpj.2010.04.054"],["dc.identifier.gro","3142885"],["dc.identifier.isi","000280182300042"],["dc.identifier.pmid","20643088"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/338"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","High- and Low-Mobility Stages in the Synaptic Vesicle Cycle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The Journal of General Physiology"],["dc.bibliographiccitation.volume","136"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Wagner, Edward J."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hagen, Brian M."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T08:41:47Z"],["dc.date.available","2018-11-07T08:41:47Z"],["dc.date.issued","2010"],["dc.format.extent","9A"],["dc.identifier.isi","000279473500028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19548"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Rockefeller Univ Press"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Marine Biol Lab, Woods Hole, MA"],["dc.relation.issn","0022-1295"],["dc.title","Super-Resolution Imaging of Cardiac Signaling Microdomains by STED Microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","417"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Biophotonics"],["dc.bibliographiccitation.lastpage","424"],["dc.bibliographiccitation.volume","3"],["dc.bibliographiccitation.volumetitle","Advanced Micro and Nanoscopy for Biomedicine"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:45:57Z"],["dc.date.available","2017-09-07T11:45:57Z"],["dc.date.issued","2010"],["dc.description.abstract","We compare the performance of video-rate Stimulated Emission Depletion (STED) and confocal microscopy in imaging the interior of living neurons. A lateral resolution of 65 nm is observed in STED movies of 28 frames per second, which is 4-fold higher in spatial resolution than in their confocal counterparts. S FED microscopy, but not confocal microscopy, allows discrimination of single features at high spatial densities. Specific patterns of movement within the confined space of the axon are revealed in STED microscopy, while confocal imaging is limited to reporting gross motion. Further progress is to be expected, as we demonstrate that the use of continuous wave (CW) beams for excitation and STED is viable for video-rate STED recording of living neurons. Tentatively providing a larger photon flux, CW beams should facilitate extending fast STED imaging towards imaging fainter living samples. [GRAPHICS] Synaptic vesicles within an axon. Single frames of movies recorded at 28 frames per second. Confocal microscopy (left) can only reproduce the axon, whereas STED microscopy (right) can resolve moving objects in the neuron; '+' indicates that the data are linearly deconvolved."],["dc.identifier.doi","10.1002/jbio.201000038"],["dc.identifier.gro","3142897"],["dc.identifier.isi","000279969600003"],["dc.identifier.pmid","20379984"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/352"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1864-063X"],["dc.title","Comparing video-rate STED nanoscopy and confocal microscopy of living neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","223A"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","224A"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Wagner, Eva M."],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Streich, Jan-Hendrik"],["dc.contributor.author","Tuan, Hoang-Trong M."],["dc.contributor.author","Hagen, Brian M."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Jafri, Mohsin S."],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T09:14:13Z"],["dc.date.available","2018-11-07T09:14:13Z"],["dc.date.issued","2012"],["dc.identifier.isi","000321561201431"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27356"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","0006-3495"],["dc.title","Live Cell Super-Resolution Imaging of Transverse Membrane Tubules in Heart Failure"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","19055"],["dc.bibliographiccitation.issue","44"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","19060"],["dc.bibliographiccitation.volume","107"],["dc.contributor.author","Hoopmann, Peer"],["dc.contributor.author","Punge, Annedore"],["dc.contributor.author","Barysch, Sina Victoria"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Bückers, Johanna"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Bethani, Ioanna"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Rizzoli, Silvio"],["dc.date.accessioned","2017-09-07T11:45:12Z"],["dc.date.available","2017-09-07T11:45:12Z"],["dc.date.issued","2010"],["dc.description.abstract","Neurotransmitter release is achieved through the fusion of synaptic vesicles with the neuronal plasma membrane (exocytosis). Vesicles are then retrieved from the plasma membrane (endocytosis). It was hypothesized more than 3 decades ago that endosomes participate in vesicle recycling, constituting a slow endocytosis pathway required especially after prolonged stimulation. This recycling model predicts that newly endocytosed vesicles fuse with an endosome, which sorts (organizes) the molecules and buds exocytosis-competent vesicles. We analyzed here the endosome function using hippocampal neurons, isolated nerve terminals (synaptosomes), and PC12 cells by stimulated emission depletion microscopy, photooxidation EM, and several conventional microscopy assays. Surprisingly, we found that endosomal sorting is a rapid pathway, which appeared to be involved in the recycling of the initial vesicles to be released on stimulation, the readily releasable pool. In agreement with the endosomal model, the vesicle composition changed after endocytosis, with the newly formed vesicles being enriched in plasma membrane proteins. Vesicle proteins were organized in clusters both in the plasma membrane (on exocytosis) and in the endosome. In the latter compartment, they segregated from plasma membrane components in a process that is likely important for sorting/budding of newly developed vesicles from the endosome."],["dc.identifier.doi","10.1073/pnas.1007037107"],["dc.identifier.gro","3142833"],["dc.identifier.isi","000283749000058"],["dc.identifier.pmid","20956291"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/281"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0027-8424"],["dc.title","Endosomal sorting of readily releasable synaptic vesicles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","402"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","414"],["dc.bibliographiccitation.volume","111"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Streich, Jan-Hendrik"],["dc.contributor.author","Korff, Brigitte"],["dc.contributor.author","Tuan, Hoang-Trong M."],["dc.contributor.author","Hagen, Brian"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Jafri, M. Saleet"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2017-09-07T11:48:29Z"],["dc.date.available","2017-09-07T11:48:29Z"],["dc.date.issued","2012"],["dc.description.abstract","Rationale: Transverse tubules (TTs) couple electric surface signals to remote intracellular Ca2+ release units (CRUs). Diffraction-limited imaging studies have proposed loss of TT components as disease mechanism in heart failure (HF). Objectives: Objectives were to develop quantitative super-resolution strategies for live-cell imaging of TT membranes in intact cardiomyocytes and to show that TT structures are progressively remodeled during HF development, causing early CRU dysfunction. Methods and Results: Using stimulated emission depletion (STED) microscopy, we characterized individual TTs with nanometric resolution as direct readout of local membrane morphology 4 and 8 weeks after myocardial infarction (4pMI and 8pMI). Both individual and network TT properties were investigated by quantitative image analysis. The mean area of TT cross sections increased progressively from 4pMI to 8pMI. Unexpectedly, intact TT networks showed differential changes. Longitudinal and oblique TTs were significantly increased at 4pMI, whereas transversal components appeared decreased. Expression of TT-associated proteins junctophilin-2 and caveolin-3 was significantly changed, correlating with network component remodeling. Computational modeling of spatial changes in HF through heterogeneous TT reorganization and RyR2 orphaning (5000 of 20 000 CRUs) uncovered a local mechanism of delayed subcellular Ca2+ release and action potential prolongation. Conclusions: This study introduces STED nanoscopy for live mapping of TT membrane structures. During early HF development, the local TT morphology and associated proteins were significantly altered, leading to differential network remodeling and Ca2+ release dyssynchrony. Our data suggest that TT remodeling during HF development involves proliferative membrane changes, early excitation-contraction uncoupling, and network fracturing."],["dc.identifier.doi","10.1161/CIRCRESAHA.112.274530"],["dc.identifier.gro","3142487"],["dc.identifier.isi","000307308700007"],["dc.identifier.pmid","22723297"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8829"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/73"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A05: Molekulares Imaging von kardialen Calcium-Freisetzungsdomänen"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.issn","0009-7330"],["dc.relation.workinggroup","RG Hasenfuß (Transition zur Herzinsuffizienz)"],["dc.relation.workinggroup","RG Hell"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.title","Stimulated Emission Depletion Live-Cell Super-Resolution Imaging Shows Proliferative Remodeling of T-Tubule Membrane Structures After Myocardial Infarction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Hoang-Trong Minh Tuan, Hoang-Trong Minh Tuan"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Jafri, Mohsin S."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T09:14:13Z"],["dc.date.available","2018-11-07T09:14:13Z"],["dc.date.issued","2012"],["dc.format.extent","305A"],["dc.identifier.isi","000321561202133"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27357"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","0006-3495"],["dc.title","STED Nanoscopy of Cardiac RyR2 Clusters and Sub-Structure Analysis After Myocardial Infarction"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Wagner, Eva M."],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hagen, Brian M."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T08:47:09Z"],["dc.date.available","2018-11-07T08:47:09Z"],["dc.date.issued","2010"],["dc.format.extent","5A"],["dc.identifier.isi","000208762000029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20875"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.issn","0006-3495"],["dc.title","Sted Based Super-Resolution Imaging of Transverse Tubules in Ventricular Myocytes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","823"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Experimental Hematology"],["dc.bibliographiccitation.lastpage","831"],["dc.bibliographiccitation.volume","41"],["dc.contributor.author","Wilk, C. Matthias"],["dc.contributor.author","Schildberg, Frank A."],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Cadeddu, Ron-Patrick"],["dc.contributor.author","Fröbel, Julia"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Tolba, René H."],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Czibere, Akos"],["dc.contributor.author","Bruns, Ingmar"],["dc.contributor.author","Haas, Rainer"],["dc.date.accessioned","2017-09-07T11:47:10Z"],["dc.date.available","2017-09-07T11:47:10Z"],["dc.date.issued","2013"],["dc.description.abstract","Homing and engraftment of hematopoietic stem and progenitor cells (HSPCs) during bone marrow transplantation are critically dependent on integrins such as beta(1)-integrin. In the present study, we show that beta(1)-integrin and the tetraspanin CD63 form a cell surface receptor complex for the soluble serum protein tissue inhibitor of metalloproteinases-1 (TIMP-1) on human CD34(+) HSPCs. Through binding to this receptor complex, TIMP-1 activates beta(1)-integrin, increases adhesion and migration of human CD34(+) cells, and protects these cells from induced apoptosis. TIMP-1 stimulation in murine bone marrow mononuclear cells also promotes migration and adhesion; this is associated with augmented homing of murine mononuclear cells and of murine LSK+ cells during bone marrow transplantation. These results not only indicate that TIMP-1 is conducive to HSPC homing; they also identify CD63 and beta(1)-integrin as a TIMP-1 receptor complex on HSPCs."],["dc.identifier.doi","10.1016/j.exphem.2013.04.010"],["dc.identifier.gro","3142289"],["dc.identifier.isi","000324359200009"],["dc.identifier.pmid","23660069"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6631"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0301-472X"],["dc.title","The tissue inhibitor of metalloproteinases-1 improves migration and adhesion of hematopoietic stem and progenitor cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]