Darienko, Tatyana M.

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Pröschold, Thomas"],["dc.contributor.author","Rieser, Daniel"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Nachbaur, Laura"],["dc.contributor.author","Kammerlander, Barbara"],["dc.contributor.author","Qian, Kuimei"],["dc.contributor.author","Pitsch, Gianna"],["dc.contributor.author","Bruni, Estelle Patricia"],["dc.contributor.author","Qu, Zhishuai"],["dc.contributor.author","Forster, Dominik"],["dc.contributor.author","Rad-Menendez, Cecilia"],["dc.contributor.author","Posch, Thomas"],["dc.contributor.author","Stoeck, Thorsten"],["dc.contributor.author","Sonntag, Bettina"],["dc.date.accessioned","2021-04-14T08:28:35Z"],["dc.date.available","2021-04-14T08:28:35Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1038/s41598-021-84265-y"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82654"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2045-2322"],["dc.title","An integrative approach sheds new light onto the systematics and ecology of the widespread ciliate genus Coleps (Ciliophora, Prostomatea)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","811"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Systematics and Biodiversity"],["dc.bibliographiccitation.lastpage","829"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Menéndez, Cecilia Rad"],["dc.contributor.author","Campbell, Christine"],["dc.contributor.author","Pröschold, Thomas"],["dc.date.accessioned","2020-04-03T13:03:31Z"],["dc.date.available","2020-04-03T13:03:31Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1080/14772000.2019.1690597"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63608"],["dc.relation.issn","1477-2000"],["dc.relation.issn","1478-0933"],["dc.title","Are there any true marine Chlorella species? Molecular phylogenetic assessment and ecology of marine Chlorella-like organisms, including a description of Droopiella gen. nov"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1963"],["dc.bibliographiccitation.journal","Proceedings of the Royal Society of London. Series B, Biological Sciences"],["dc.bibliographiccitation.volume","288"],["dc.contributor.author","Irisarri, Iker"],["dc.contributor.author","Darienko, Tatyana M."],["dc.contributor.author","Pröschold, Thomas"],["dc.contributor.author","Fürst-Jansen, Janine M. R."],["dc.contributor.author","Jamy, Mahwash"],["dc.contributor.author","de Vries, Jan"],["dc.date.accessioned","2021-12-01T09:21:07Z"],["dc.date.available","2021-12-01T09:21:07Z"],["dc.date.issued","2021"],["dc.description.abstract","Streptophytes are one of the major groups of the green lineage (Chloroplastida or Viridiplantae). During one billion years of evolution, streptophytes have radiated into an astounding diversity of uni- and multicellular green algae as well as land plants. Most divergent from land plants is a clade formed by Mesostigmatophyceae, Spirotaenia spp. and Chlorokybophyceae. All three lineages are species-poor and the Chlorokybophyceae consist of a single described species, Chlorokybus atmophyticus. In this study, we used phylogenomic analyses to shed light into the diversity within Chlorokybus using a sampling of isolates across its known distribution. We uncovered a consistent deep genetic structure within the Chlorokybus isolates, which prompted us to formally extend the Chlorokybophyceae by describing four new species. Gene expression differences among Chlorokybus species suggest certain constitutive variability that might influence their response to environmental factors. Failure to account for this diversity can hamper comparative genomic studies aiming to understand the evolution of stress response across streptophytes. Our data highlight that future studies on the evolution of plant form and function can tap into an unknown diversity at key deep branches of the streptophytes."],["dc.description.abstract","Streptophytes are one of the major groups of the green lineage (Chloroplastida or Viridiplantae). During one billion years of evolution, streptophytes have radiated into an astounding diversity of uni- and multicellular green algae as well as land plants. Most divergent from land plants is a clade formed by Mesostigmatophyceae, Spirotaenia spp. and Chlorokybophyceae. All three lineages are species-poor and the Chlorokybophyceae consist of a single described species, Chlorokybus atmophyticus. In this study, we used phylogenomic analyses to shed light into the diversity within Chlorokybus using a sampling of isolates across its known distribution. We uncovered a consistent deep genetic structure within the Chlorokybus isolates, which prompted us to formally extend the Chlorokybophyceae by describing four new species. Gene expression differences among Chlorokybus species suggest certain constitutive variability that might influence their response to environmental factors. Failure to account for this diversity can hamper comparative genomic studies aiming to understand the evolution of stress response across streptophytes. Our data highlight that future studies on the evolution of plant form and function can tap into an unknown diversity at key deep branches of the streptophytes."],["dc.identifier.doi","10.1098/rspb.2021.2168"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94351"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","1471-2954"],["dc.relation.issn","0962-8452"],["dc.title","Unexpected cryptic species among streptophyte algae most distant to land plants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Spanner, Christian"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Filker, Sabine"],["dc.contributor.author","Sonntag, Bettina"],["dc.contributor.author","Pröschold, Thomas"],["dc.date.accessioned","2022-12-01T08:30:55Z"],["dc.date.available","2022-12-01T08:30:55Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\r\n \r\n Paramecium bursaria\r\n is a mixotrophic ciliate species, which is common in stagnant and slow-flowing, nutrient-rich waters. It is usually found living in symbiosis with\r\n zoochlorellae\r\n (green algae) of the genera\r\n Chlorella\r\n or\r\n Micractinium\r\n . We investigated\r\n P. bursaria\r\n isolates from around the world, some of which have already been extensively studied in various laboratories, but whose morphological and genetic identity has not yet been completely clarified. Phylogenetic analyses of the SSU and ITS rDNA sequences revealed five highly supported lineages, which corresponded to the syngen and most likely to the biological species assignment. These syngens R1–R5 could also be distinguished by unique synapomorphies in the secondary structures of the SSU and the ITS. Considering these synapomorphies, we could clearly assign the existing GenBank entries of\r\n P. bursaria\r\n to specific syngens. In addition, we discovered synapomorphies at amino acids of the COI gene for the identification of the syngens. Using the metadata of these entries, most syngens showed a worldwide distribution, however, the syngens R1 and R5 were only found in Europe. From morphology, the syngens did not show any significant deviations. The investigated strains had either\r\n Chlorella variabilis\r\n ,\r\n Chlorella vulgaris\r\n or\r\n Micractinium conductrix\r\n as endosymbionts."],["dc.identifier.doi","10.1038/s41598-022-22284-z"],["dc.identifier.pii","22284"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118019"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","2045-2322"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Morphological diversity and molecular phylogeny of five Paramecium bursaria (Alveolata, Ciliophora, Oligohymenophorea) syngens and the identification of their green algal endosymbionts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2327"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Applied Phycology"],["dc.bibliographiccitation.lastpage","2340"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Reichelt, Niklas"],["dc.contributor.author","Leger, Dorian"],["dc.contributor.author","Daubert, Mareike"],["dc.contributor.author","Ruffino, Paolo"],["dc.contributor.author","Pröschold, Thomas"],["dc.contributor.author","Darienko, Tatyana"],["dc.date.accessioned","2021-08-12T07:46:13Z"],["dc.date.available","2021-08-12T07:46:13Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Chromochloris zofingiensis (Dönz) Fucíková & L.A.Lewis, due to its production of highly valuable carotenoids such as astaxanthin, is a model organism in biotechnology. Since the recognition of this physiological property, many biotechnological applications have only used a single strain (SAG 211-14 = CCAP 211/14 = UTEX 32 = ATCC 30412) to produce biomass and carotenoids. However, multiple acquisitions of strains putatively belonging to the same species raised the question of the conspecificity of those strains and their properties. In this study, the conspecificity of the available strains, which are deposited axenically in SAG, was tested using SSU and ITS rDNA sequencing and AFLP ( Eco RI/ Pst I) analyses. The comparison of SSU and ITS rDNA sequences as well as the AFLP patterns revealed that the investigated strains formed two very similar groups, (1) SAG 211-14, SAG 4.80, SAG 31.80, and SAG 34.80 and (2) SAG 221-2. All strains belonged to one species, C. zofingiensis , and represented one monophyletic lineage within the so-called DO-group of the Chlorophyceae. The robustness to cryopreservation and the subsequent epigenetic variability was detected using the methylation-sensitive AFLP ( Eco RI/ Msp I and Eco RI/ Hpa II) among the five Chromochloris strains. All strains showed a high rate of survival (54.4–98.1%) during cryopreservation. The methylation patterns varied between precryo and postcryo in all strains detected among three time points (before, shortly after, and 8 weeks after cryopreservation), showing that the MS-AFLP technique has the potential to detect epigenetic effects occurring in response to cryopreservation and other stresses. Finally, the potential of these five strains for usage in biotechnological applications was proven by growing them in aerated cultures with and without additional carbon dioxide supply. The comparison showed that all strains produced high amounts of biomass and carotenoids under aeration with additional CO 2 and were therefore suitable in biotechnology."],["dc.description.abstract","Abstract Chromochloris zofingiensis (Dönz) Fucíková & L.A.Lewis, due to its production of highly valuable carotenoids such as astaxanthin, is a model organism in biotechnology. Since the recognition of this physiological property, many biotechnological applications have only used a single strain (SAG 211-14 = CCAP 211/14 = UTEX 32 = ATCC 30412) to produce biomass and carotenoids. However, multiple acquisitions of strains putatively belonging to the same species raised the question of the conspecificity of those strains and their properties. In this study, the conspecificity of the available strains, which are deposited axenically in SAG, was tested using SSU and ITS rDNA sequencing and AFLP ( Eco RI/ Pst I) analyses. The comparison of SSU and ITS rDNA sequences as well as the AFLP patterns revealed that the investigated strains formed two very similar groups, (1) SAG 211-14, SAG 4.80, SAG 31.80, and SAG 34.80 and (2) SAG 221-2. All strains belonged to one species, C. zofingiensis , and represented one monophyletic lineage within the so-called DO-group of the Chlorophyceae. The robustness to cryopreservation and the subsequent epigenetic variability was detected using the methylation-sensitive AFLP ( Eco RI/ Msp I and Eco RI/ Hpa II) among the five Chromochloris strains. All strains showed a high rate of survival (54.4–98.1%) during cryopreservation. The methylation patterns varied between precryo and postcryo in all strains detected among three time points (before, shortly after, and 8 weeks after cryopreservation), showing that the MS-AFLP technique has the potential to detect epigenetic effects occurring in response to cryopreservation and other stresses. Finally, the potential of these five strains for usage in biotechnological applications was proven by growing them in aerated cultures with and without additional carbon dioxide supply. The comparison showed that all strains produced high amounts of biomass and carotenoids under aeration with additional CO 2 and were therefore suitable in biotechnology."],["dc.identifier.doi","10.1007/s10811-021-02468-2"],["dc.identifier.pii","2468"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88649"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","1573-5176"],["dc.relation.issn","0921-8971"],["dc.title","Epigenomic stability assessment during cryopreservation and physiology among various strains of Chromochloris zofingiensis (Chlorophyceae) and their genetic variability revealed by AFLP and MS-AFLP"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1586"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Microorganisms"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Rad-Menéndez, Cecilia"],["dc.contributor.author","Campbell, Christine N."],["dc.contributor.author","Pröschold, Thomas"],["dc.date.accessioned","2021-10-01T09:58:30Z"],["dc.date.available","2021-10-01T09:58:30Z"],["dc.date.issued","2021"],["dc.description.abstract","Most marine coccoid and sarcinoid green algal species have traditionally been placed within genera dominated by species from freshwater or soil habitats. For example, the genera Chlorocystis and Halochlorococcum contain exclusively marine species; however, their familial and ordinal affinities are unclear. They are characterized by a vegetative cell with lobated or reticulated chloroplast, formation of quadriflagellated zoospores and living epi- or endophytically within benthic macroalgae. They were integrated into the family Chlorochytriaceae which embraces all coccoid green algae with epi- or endophytic life phases. Later, they were excluded from the family of Chlorococcales based on studies of their life histories in culture, and transferred to their newly described order, Chlorocystidales of the Ulvophyceae. Both genera form a “Codiolum”-stage that serves as the unicellular sporophyte in their life cycles. Phylogenetic analyses of SSU and ITS rDNA sequences confirmed that these coccoid taxa belong to the Chlorocystidales, together with the sarcinoid genus Desmochloris. The biflagellated coccoid strains were members of the genus Sykidion, which represented its own order, Sykidiales, among the Ulvophyceae. Considering these results and the usage of the ITS-2/CBC approach revealed three species of Desmochloris, six of Chlorocystis, and three of Sykidion. Three new species and several new combinations were proposed."],["dc.description.abstract","Most marine coccoid and sarcinoid green algal species have traditionally been placed within genera dominated by species from freshwater or soil habitats. For example, the genera Chlorocystis and Halochlorococcum contain exclusively marine species; however, their familial and ordinal affinities are unclear. They are characterized by a vegetative cell with lobated or reticulated chloroplast, formation of quadriflagellated zoospores and living epi- or endophytically within benthic macroalgae. They were integrated into the family Chlorochytriaceae which embraces all coccoid green algae with epi- or endophytic life phases. Later, they were excluded from the family of Chlorococcales based on studies of their life histories in culture, and transferred to their newly described order, Chlorocystidales of the Ulvophyceae. Both genera form a “Codiolum”-stage that serves as the unicellular sporophyte in their life cycles. Phylogenetic analyses of SSU and ITS rDNA sequences confirmed that these coccoid taxa belong to the Chlorocystidales, together with the sarcinoid genus Desmochloris. The biflagellated coccoid strains were members of the genus Sykidion, which represented its own order, Sykidiales, among the Ulvophyceae. Considering these results and the usage of the ITS-2/CBC approach revealed three species of Desmochloris, six of Chlorocystis, and three of Sykidion. Three new species and several new combinations were proposed."],["dc.identifier.doi","10.3390/microorganisms9081586"],["dc.identifier.pii","microorganisms9081586"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/90076"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation.eissn","2076-2607"],["dc.title","Molecular Phylogeny of Unicellular Marine Coccoid Green Algae Revealed New Insights into the Systematics of the Ulvophyceae (Chlorophyta)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","493"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Phycology"],["dc.bibliographiccitation.lastpage","499"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Pröschold, Thomas"],["dc.contributor.editor","De Clerck, O."],["dc.date.accessioned","2020-12-10T18:29:05Z"],["dc.date.available","2020-12-10T18:29:05Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1111/jpy.12830"],["dc.identifier.eissn","1529-8817"],["dc.identifier.issn","0022-3646"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76521"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Reevaluation and discovery of new species of the rare genus Watanabea and establishment of Massjukichlorella gen. nov. (Trebouxiophyceae, Chlorophyta) using an integrative approach"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","175"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Symbiosis"],["dc.bibliographiccitation.lastpage","188"],["dc.bibliographiccitation.volume","82"],["dc.contributor.author","Pröschold, Thomas"],["dc.contributor.author","Darienko, Tatyana"],["dc.date.accessioned","2021-04-14T08:23:32Z"],["dc.date.available","2021-04-14T08:23:32Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/s13199-020-00711-x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80958"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1878-7665"],["dc.relation.issn","0334-5114"],["dc.title","Choricystis and Lewiniosphaera gen. nov. (Trebouxiophyceae Chlorophyta), two different green algal endosymbionts in freshwater sponges"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]