Belousov, Vsevolod V.

[["dc.bibliographiccitation.firstpage","4200"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","20"],["dc.contributor.affiliation","Kostyuk, Alexander I.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia, \t\t \r\n\t\t Pirogov Russian National Research Medical University, Moscow 117997, Russia,"],["dc.contributor.affiliation","Demidovich, Aleksandra D.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia,"],["dc.contributor.affiliation","Kotova, Daria A.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia,"],["dc.contributor.affiliation","Belousov, Vsevolod V.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia, \t\t \r\n\t\t Pirogov Russian National Research Medical University, Moscow 117997, Russia, \t\t \r\n\t\t Institute for Cardiovascular Physiology, Georg August University Göttingen, D-37073 Göttingen, Germany,"],["dc.contributor.affiliation","Bilan, Dmitry S.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia, d.s.bilan@gmail.com\t\t \r\n\t\t Pirogov Russian National Research Medical University, Moscow 117997, Russia, d.s.bilan@gmail.com"],["dc.contributor.author","Kostyuk, Alexander I."],["dc.contributor.author","Demidovich, Aleksandra D."],["dc.contributor.author","Kotova, Daria A."],["dc.contributor.author","Belousov, Vsevolod V."],["dc.contributor.author","Bilan, Dmitry S."],["dc.date.accessioned","2020-12-10T18:47:09Z"],["dc.date.available","2020-12-10T18:47:09Z"],["dc.date.issued","2019"],["dc.date.updated","2022-09-05T16:13:15Z"],["dc.identifier.doi","10.3390/ijms20174200"],["dc.identifier.eissn","1422-0067"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16625"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78660"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","1422-0067"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","febs.16088"],["dc.bibliographiccitation.firstpage","5382"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","The FEBS Journal"],["dc.bibliographiccitation.lastpage","5395"],["dc.bibliographiccitation.volume","289"],["dc.contributor.affiliation","Smolyarova, Daria D.; 1\r\nShemyakin‐Ovchinnikov Institute of Bioorganic Chemistry\r\nMoscow Russia"],["dc.contributor.affiliation","Podgorny, Oleg V.; 1\r\nShemyakin‐Ovchinnikov Institute of Bioorganic Chemistry\r\nMoscow Russia"],["dc.contributor.affiliation","Bilan, Dmitry S.; 1\r\nShemyakin‐Ovchinnikov Institute of Bioorganic Chemistry\r\nMoscow Russia"],["dc.contributor.author","Smolyarova, Daria D."],["dc.contributor.author","Podgorny, Oleg V."],["dc.contributor.author","Bilan, Dmitry S."],["dc.contributor.author","Belousov, Vsevolod V."],["dc.date.accessioned","2022-11-28T09:43:24Z"],["dc.date.available","2022-11-28T09:43:24Z"],["dc.date.issued","2021"],["dc.date.updated","2022-11-27T10:11:15Z"],["dc.description.abstract","Cell metabolism heavily relies on the redox reactions that inevitably generate reactive oxygen species (ROS). It is now well established that ROS fluctuations near basal levels coordinate numerous physiological processes in living organisms, thus exhibiting regulatory functions. Hydrogen peroxide, the most long‐lived ROS, is a key contributor to ROS‐dependent signal transduction in the cell. H2O2 is known to impact various targets in the cell; therefore, the question of how H2O2 modulates physiological processes in a highly specific manner is central in redox biology. To resolve this question, novel genetic tools have recently been created for detecting H2O2 and emulating its generation in living organisms with unmatched spatiotemporal resolution. Here, we review H2O2‐sensitive genetically encoded fluorescent sensors and opto‐ and chemogenetic tools for controlled H2O2 generation."],["dc.description.abstract","Hydrogen peroxide (H2O2) is one of the most important signaling molecules, which coordinates many physiological processes ranging from development to wound healing. Identification of mechanisms underlying specificity of H2O2‐dependent signal transduction is a central issue in redox biology. A palette of genetically encoded tools for generation and detection of H2O2 with unprecedented spatiotemporal resolution has been recently created to resolve this issue. Here, we present an overview of these tools.\r\n\r\nimage"],["dc.description.sponsorship","Ministry of Science and Higher Education of the Russian Federation"],["dc.description.sponsorship","Russian Science Foundation http://dx.doi.org/10.13039/501100006769"],["dc.identifier.doi","10.1111/febs.16088"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/117308"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.relation.eissn","1742-4658"],["dc.relation.issn","1742-464X"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made."],["dc.title","A guide to genetically encoded tools for the study of H2O2"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","516"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Antioxidants"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Kostyuk, Alexander I."],["dc.contributor.author","Kokova, Aleksandra D."],["dc.contributor.author","Podgorny, Oleg V."],["dc.contributor.author","Kelmanson, Ilya V."],["dc.contributor.author","Fetisova, Elena S."],["dc.contributor.author","Belousov, Vsevolod V."],["dc.contributor.author","Bilan, Dmitry S."],["dc.date.accessioned","2021-04-14T08:25:08Z"],["dc.date.available","2021-04-14T08:25:08Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Russian Science Foundation"],["dc.description.sponsorship","Russian Foundation for Basic Research"],["dc.description.sponsorship","Ministry of Science and Higher Education of the Russian Federation"],["dc.identifier.doi","10.3390/antiox9060516"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81530"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-3921"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Genetically Encoded Tools for Research of Cell Signaling and Metabolism under Brain Hypoxia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","731"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Antioxidants"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","de Cubas, Laura"],["dc.contributor.author","Pak, Valeriy V."],["dc.contributor.author","Belousov, Vsevolod V."],["dc.contributor.author","Ayté, José"],["dc.contributor.author","Hidalgo, Elena"],["dc.date.accessioned","2021-06-01T09:42:31Z"],["dc.date.available","2021-06-01T09:42:31Z"],["dc.date.issued","2021"],["dc.description.abstract","Fluorescent protein-based reporters used to measure intracellular H2O2 were developed to overcome the limitations of small permeable dyes. The two major families of genetically encoded redox reporters are the reduction-oxidation sensitive green fluorescent protein (roGFP)-based proteins fused to peroxiredoxins and HyPer and derivatives. We have used the most sensitive probes of each family, roGFP2-Tpx1.C169S and HyPer7, to monitor steady-state and fluctuating levels of peroxides in fission yeast. While both are able to monitor the nanomolar fluctuations of intracellular H2O2, the former is two-five times more sensitive than HyPer7, and roGFP2-Tpx1.C169S is partially oxidized in the cytosol of wild-type cells while HyPer7 is fully reduced. We have successfully expressed HyPer7 in the mitochondrial matrix, and it is ~40% oxidized, suggesting higher steady-state levels of peroxides, in the low micromolar range, than in the cytosol. Cytosolic HyPer7 can detect negligible H2O2 in the cytosol from mitochondrial origin unless the main H2O2 scavenger, the cytosolic peroxiredoxin Tpx1, is absent, while mitochondrial HyPer7 is oxidized to the same extent in wild-type and ∆tpx1 cells. We conclude that there is a bidirectional flux of H2O2 across the matrix and the cytosol, but Tpx1 rapidly and efficiently scavenges mitochondrial-generated peroxides and stops their steady-state cytosolic levels rising."],["dc.description.sponsorship","Ministerio de Ciencia, Innovación y Universidades, Spain"],["dc.identifier.doi","10.3390/antiox10050731"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85274"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-3921"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","The Mitochondria-to-Cytosol H2O2 Gradient Is Caused by Peroxiredoxin-Dependent Cytosolic Scavenging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","8164"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","21"],["dc.contributor.affiliation","Kostyuk, Alexander I.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia, alexander.kostyuk@inbox.ru\t\t \r\n\t\t Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia, alexander.kostyuk@inbox.ru"],["dc.contributor.affiliation","Panova, Anastasiya S.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia, panova_a@ymail.com\t\t \r\n\t\t Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia, panova_a@ymail.com"],["dc.contributor.affiliation","Kokova, Aleksandra D.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia, ms.demidovich@inbox.ru\t\t \r\n\t\t Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia, ms.demidovich@inbox.ru"],["dc.contributor.affiliation","Kotova, Daria A.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia, kotovadaria95@gmail.com\t\t \r\n\t\t Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia, kotovadaria95@gmail.com"],["dc.contributor.affiliation","Maltsev, Dmitry I.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia, mal-dima@yandex.ru\t\t \r\n\t\t Federal Center for Cerebrovascular Pathology and Stroke, 117997 Moscow, Russia, mal-dima@yandex.ru"],["dc.contributor.affiliation","Podgorny, Oleg V.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia, olegpodgorny@inbox.ru\t\t \r\n\t\t Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia, olegpodgorny@inbox.ru"],["dc.contributor.affiliation","Belousov, Vsevolod V.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia, belousov@fccps.ru\t\t \r\n\t\t Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia, belousov@fccps.ru\t\t \r\n\t\t Federal Center for Cerebrovascular Pathology and Stroke, 117997 Moscow, Russia, belousov@fccps.ru\t\t \r\n\t\t Institute for Cardiovascular Physiology, Georg August University Göttingen, D-37073 Göttingen, Germany, belousov@fccps.ru"],["dc.contributor.affiliation","Bilan, Dmitry S.; \t\t \r\n\t\t Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia, d.s.bilan@gmail.com\t\t \r\n\t\t Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia, d.s.bilan@gmail.com"],["dc.contributor.author","Kostyuk, Alexander I."],["dc.contributor.author","Panova, Anastasiya S."],["dc.contributor.author","Kokova, Aleksandra D."],["dc.contributor.author","Kotova, Daria A."],["dc.contributor.author","Maltsev, Dmitry I."],["dc.contributor.author","Podgorny, Oleg V."],["dc.contributor.author","Belousov, Vsevolod V."],["dc.contributor.author","Bilan, Dmitry S."],["dc.date.accessioned","2021-04-14T08:31:06Z"],["dc.date.available","2021-04-14T08:31:06Z"],["dc.date.issued","2020"],["dc.date.updated","2022-09-06T17:40:57Z"],["dc.identifier.doi","10.3390/ijms21218164"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83485"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1422-0067"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","In Vivo Imaging with Genetically Encoded Redox Biosensors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","15362"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Ermakova, Yulia G."],["dc.contributor.author","Lanin, Aleksandr A."],["dc.contributor.author","Fedotov, Ilya V."],["dc.contributor.author","Roshchin, Matvey"],["dc.contributor.author","Kelmanson, Ilya V."],["dc.contributor.author","Kulik, Dmitry"],["dc.contributor.author","Bogdanova, Yulia A."],["dc.contributor.author","Shokhina, Arina G."],["dc.contributor.author","Bilan, Dmitry S."],["dc.contributor.author","Staroverov, Dmitry B."],["dc.contributor.author","Balaban, Pavel M."],["dc.contributor.author","Fedotov, Andrei B."],["dc.contributor.author","Sidorov-Biryukov, Dmitry A."],["dc.contributor.author","Nikitin, Evgeny S."],["dc.contributor.author","Zheltikov, Aleksei M."],["dc.contributor.author","Belousov, Vsevolod V."],["dc.date.accessioned","2018-11-07T10:23:42Z"],["dc.date.available","2018-11-07T10:23:42Z"],["dc.date.issued","2017"],["dc.description.abstract","Thermogenetics is a promising innovative neurostimulation technique, which enables robust activation of neurons using thermosensitive transient receptor potential (TRP) cation channels. Broader application of this approach in neuroscience is, however, hindered by a limited variety of suitable ion channels, and by low spatial and temporal resolution of neuronal activation when TRP channels are activated by ambient temperature variations or chemical agonists. Here, we demonstrate rapid, robust and reproducible repeated activation of snake TRPA1 channels heterologously expressed in non-neuronal cells, mouse neurons and zebrafish neurons in vivo by infrared (IR) laser radiation. A fibre-optic probe that integrates a nitrogen - vacancy (NV) diamond quantum sensor with optical and microwave waveguide delivery enables thermometry with single-cell resolution, allowing neurons to be activated by exceptionally mild heating, thus preventing the damaging effects of excessive heat. The neuronal responses to the activation by IR laser radiation are fully characterized using Ca2+ imaging and electrophysiology, providing, for the first time, a complete framework for a thermogenetic manipulation of individual neurons using IR light."],["dc.identifier.doi","10.1038/ncomms15362"],["dc.identifier.isi","000401907500001"],["dc.identifier.pmid","28530239"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14904"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42512"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Thermogenetic neurostimulation with single-cell resolution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Steinhorn, Benjamin"],["dc.contributor.author","Sorrentino, Andrea"],["dc.contributor.author","Badole, Sachin"],["dc.contributor.author","Bogdanova, Yulia"],["dc.contributor.author","Belousov, Vsevolod"],["dc.contributor.author","Michel, Thomas"],["dc.date.accessioned","2020-12-10T18:09:49Z"],["dc.date.available","2020-12-10T18:09:49Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1038/s41467-018-06533-2"],["dc.identifier.eissn","2041-1723"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15613"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73765"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Chemogenetic generation of hydrogen peroxide in the heart induces severe cardiac dysfunction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]