Funke, Frank

[["dc.bibliographiccitation.firstpage","724"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","European Journal of Organic Chemistry"],["dc.bibliographiccitation.lastpage","748"],["dc.contributor.author","Wu, Y."],["dc.contributor.author","Flynn, B."],["dc.contributor.author","Schirmer, H."],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Mueller, S."],["dc.contributor.author","Labahn, T."],["dc.contributor.author","Notzel, M."],["dc.contributor.author","de Meijere, Armin"],["dc.date.accessioned","2018-11-07T10:51:08Z"],["dc.date.available","2018-11-07T10:51:08Z"],["dc.date.issued","2004"],["dc.description.abstract","The beta-amino-substituted mu,beta-unsaturated Fischer carbene complexes 3 are readily available by a four step one-pot procedure from terminal alkynes, chromium hexacarbonyl and secondary amines (24 examples with yields of 68-99% and 7 examples with yields of 26-63%). The formal [3+2] cycloadditions of complexes 3 with different alkynes including diynes and enynes performed in donor solvents such as pyridine or acetonitrile afforded highly substituted 5-(dialkylamino)-3-ethoxycyclopentadienes 7, generally in medium to excellent yields (25 examples with yields of 60-95% and 7 examples with yields of 18-53%). The steric and electronic effects of the substituents on the carbene complexes and the incorporated alkynes on the regio- and stereo selectivity of the ring-forming reaction have been elaborated. An interesting 1,5- or, more likely, 1,2-migration of the dimethylamino group was observed for 5-(dimethylamino)-3-ethoxycyclopentadienes with trimethylsilyl and jPr substituents at C-5. Attempted asymmetric syntheses of cyclopentadienes 7 from complexes 3 with chiral amino groups or substituents were only moderately successful. At a center of chirality in the secondary amino group, complexes of type 3 gave compounds 7 with diastereomeric excesses of, at best, 59% in yields of 54%, and with a stereogenic center in the substituent R-1 attached to the vinyl group of 3, diastereomeric excesses as high as 94% could be achieved, but with poor chemical yields (21%). In general, cyclopentenones 21 could be easily obtained from the cyclopentadienes 7 under acidic conditions in very good yields (4 examples with yields of 81-98%, 1 example with an overall yield of 50% from complex 3). Intramolecular aldol reactions of dicarbonyl compounds generated by hydrolysis of cyclopentadienes 7 with acetal-protected aldehyde or ketone carbonyl groups in either the 5-substituent R-1 or the N-substituent R-2 led to the bicyclic compounds 22 and 23. The dimethylamino group in cyclopentenones 21 could be either eliminated or transformed into other functional groups via the quaternary ammonium salts 24. The elimination product, cyclopentadienone 27 can undergo dimerization either by a formal [4+2] or [2+2] cycloaddition. Cyclopentenone 21naaa with a bromovinyl-terminated side chain undergoes an intramolecular Heck reaction to form 5-methyl-4,6-dimethylenebicyclo[3.3.0]oct-1-en-3one (32) (37% yield). (C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004."],["dc.identifier.doi","10.1002/ejoc.200300534"],["dc.identifier.isi","000189310700007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48821"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.issn","1434-193X"],["dc.title","From alpha,beta-unsaturated Fischer carbene complexes to highly substituted 3-ethoxycyclopentadienes, masked cyclopentenones"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2395"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Neurophysiology"],["dc.bibliographiccitation.lastpage","2410"],["dc.bibliographiccitation.volume","101"],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Kron, Miriam"],["dc.contributor.author","Dutschmann, Mathias"],["dc.contributor.author","Mueller, Michael"],["dc.date.accessioned","2018-11-07T08:30:23Z"],["dc.date.available","2018-11-07T08:30:23Z"],["dc.date.issued","2009"],["dc.description.abstract","Funke F, Kron M, Dutschmann M, Muller M. Infant brain stem is prone to the generation of spreading depression during severe hypoxia. J Neurophysiol 101: 2395-2410, 2009. First published March 4, 2009; doi:10.1152/jn.91260.2008. Spreading depression (SD) resembles a concerted, massive neuronal/glial depolarization propagating within the gray matter. Being associated with cerebropathology, such as cerebral ischemia or hemorrhage, epileptic seizures, and migraine, it is well studied in cortex and hippocampus. We have now analyzed the susceptibility of rat brain stem to hypoxia-induced spreading depression-like depolarization (HSD), which could critically interfere with cardiorespiratory control. In rat brain stem slices, severe hypoxia (oxygen withdrawal) triggered HSD within minutes. The sudden extracellular DC potential shift of approximately -20 mV showed the typical profile known from other brain regions and was accompanied by an intrinsic optical signal (IOS). Spatiotemporal IOS analysis revealed that in infant brain stem, HSD was preferably ignited within the spinal trigeminal nucleus and then mostly spread out medially, invading the hypoglossal nucleus, the nucleus of the solitary tract (NTS), and the ventral respiratory group (VRG). The neuronal hypoxic depolarizations underlying the generation of HSD were massive, but incomplete. The propagation velocity of HSD and the associated extracellular K+ rise were also less marked than in other brain regions. In adult brain stem, HSD was mostly confined to the NTS and its occurrence was facilitated by hypotonic solutions, but not by glial poisoning or block of GABAergic and glycinergic synapses. In conclusion, brain stem tissue reliably generates propagating HSD episodes, which may be of interest for basilar-type migraine and brain stem infarcts. The preferred occurrence of HSD in the infant brain stem and its propagation into the VRG may be of importance for neonatal brain stem pathology such as sudden infant death syndrome."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.1152/jn.91260.2008"],["dc.identifier.isi","000265398100023"],["dc.identifier.pmid","19261708"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16886"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Physiological Soc"],["dc.relation.issn","0022-3077"],["dc.title","Infant Brain Stem Is Prone to the Generation of Spreading Depression During Severe Hypoxia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3067"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Neurophysiology"],["dc.bibliographiccitation.lastpage","3079"],["dc.bibliographiccitation.volume","105"],["dc.contributor.author","Kron, Miriam"],["dc.contributor.author","Zimmermann, Jasper L."],["dc.contributor.author","Dutschmann, Mathias"],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Müller, Michael"],["dc.date.accessioned","2018-09-28T10:28:17Z"],["dc.date.available","2018-09-28T10:28:17Z"],["dc.date.issued","2011"],["dc.description.abstract","Rett syndrome (RTT) patients suffer from respiratory arrhythmias with frequent apneas causing intermittent hypoxia. In a RTT mouse model (methyl-CpG-binding protein 2-deficient mice; Mecp2(-/y)) we recently discovered an enhanced hippocampal susceptibility to hypoxia and hypoxia-induced spreading depression (HSD). In the present study we investigated whether this also applies to infant Mecp2(-/y) brain stem, which could become life-threatening due to failure of cardiorespiratory control. HSD most reliably occurred in the nucleus of the solitary tract (NTS) and the spinal trigeminal nucleus (Sp5). HSD susceptibility of the Mecp2(-/y) NTS and Sp5 was increased on 8 mM K(+)-mediated conditioning. 5-HT(1A) receptor stimulation with 8-hydroxy-2-(di-propylamino)tetralin (8-OH-DPAT) postponed HSD by up to 40%, mediating genotype-independent protection. The deleterious impact of HSD on in vitro respiration became obvious in rhythmically active slices, where HSD propagation into the pre-Bötzinger complex (pre-BötC) immediately arrested the respiratory rhythm. Compared with wild-type, the Mecp2(-/y) pre-BötC was invaded less frequently by HSD, but if so, HSD occurred earlier. On reoxygenation, in vitro rhythms reappeared with increased frequency, which was less pronounced in Mecp2(-/y) slices. 8-OH-DPAT increased respiratory frequency but failed to postpone HSD in the pre-BötC. Repetitive hypoxia facilitated posthypoxic recovery only if HSD occurred. In 57% of Mecp2(-/y) slices, however, HSD spared the pre-BötC. Although this occasionally promoted residual hypoxic respiratory activity (\"gasping\"), it also prolonged the posthypoxic recovery, and thus the absence of central inspiratory drive, which in vivo would lengthen respiratory arrest. In view of the breathing disorders in RTTs, the increased hypoxia susceptibility of MeCP2-deficient brain stem potentially contributes to life-threatening disturbances of cardiorespiratory control."],["dc.identifier.doi","10.1152/jn.00822.2010"],["dc.identifier.pmid","21471397"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15858"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1522-1598"],["dc.title","Altered responses of MeCP2-deficient mouse brain stem to severe hypoxia"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","A369"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","The FASEB Journal"],["dc.bibliographiccitation.lastpage","A370"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Mueller, M."],["dc.contributor.author","Dutschmann, Mathias"],["dc.date.accessioned","2018-11-07T10:07:47Z"],["dc.date.available","2018-11-07T10:07:47Z"],["dc.date.issued","2006"],["dc.identifier.isi","000236206503097"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39346"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Federation Amer Soc Exp Biol"],["dc.publisher.place","Bethesda"],["dc.relation.conference","Experimental Biology 2006 Annual Meeting"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","0892-6638"],["dc.title","A novel, more complex rhythmic slice preparation containing the preBotzinger (PBC) and Kolliker-Fuse (KF) region"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","25"],["dc.bibliographiccitation.journal","ChemInform"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Wu, Yao-Ting"],["dc.contributor.author","Flynn, Bernard"],["dc.contributor.author","Schirmer, Heiko"],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Mueller, Stefan"],["dc.contributor.author","Labahn, Thomas"],["dc.contributor.author","Noetzel, Markus"],["dc.contributor.author","de Meijere, Armin"],["dc.date.accessioned","2021-12-08T12:29:15Z"],["dc.date.available","2021-12-08T12:29:15Z"],["dc.date.issued","2004"],["dc.identifier.doi","10.1002/chin.200425079"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/96009"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-476"],["dc.relation.eissn","1522-2667"],["dc.relation.issn","0931-7597"],["dc.rights.uri","http://doi.wiley.com/10.1002/tdm_license_1.1"],["dc.title","From α,β-Unsaturated Fischer Carbene Complexes to Highly Substituted 3-Ethoxycyclopentadienes, Masked Cyclopentenones."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","C508"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","AJP Cell Physiology"],["dc.bibliographiccitation.lastpage","C516"],["dc.bibliographiccitation.volume","292"],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Dutschmann, Mathias"],["dc.contributor.author","Mueller, Michael"],["dc.date.accessioned","2018-11-07T11:05:52Z"],["dc.date.available","2018-11-07T11:05:52Z"],["dc.date.issued","2007"],["dc.description.abstract","The pre-Botzinger complex (PBC) in the rostral ventrolateral medulla contains a kernel involved in respiratory rhythm generation. So far, its respiratory activity has been analyzed predominantly by electrophysiological approaches. Recent advances in fluorescence imaging now allow for the visualization of neuronal population activity in rhythmogenic networks. In the respiratory network, voltage-sensitive dyes have been used mainly, so far, but their low sensitivity prevents an analysis of activity patterns of single neurons during rhythmogenesis. We now have succeeded in using more sensitive Ca2+ imaging to study respiratory neurons in rhythmically active brain stem slices of neonatal rats. For the visualization of neuronal activity, fluo-3 was suited best in terms of neuronal specificity, minimized background fluorescence, and response magnitude. The tissue penetration of fluo-3 was improved by hyperosmolar treatment (100 mM mannitol) during dye loading. Rhythmic population activity was imaged with single-cell resolution using a sensitive charge-coupled device camera and a x20 objective, and it was correlated with extracellularly recorded mass activity of the contralateral PBC. Correlated optical neuronal activity was obvious online in 29% of slices. Rhythmic neurons located deeper became detectable during offline image processing. Based on their activity patterns, 74% of rhythmic neurons were classified as inspiratory and 26% as expiratory neurons. Our approach is well suited to visualize and correlate the activity of several single cells with respiratory network activity. We demonstrate that neuronal synchronization and possibly even network configurations can be analyzed in a noninvasive approach with single-cell resolution and at frame rates currently not reached by most scanning-based imaging techniques."],["dc.identifier.doi","10.1152/ajpcell.00253.2006"],["dc.identifier.isi","000243425900051"],["dc.identifier.pmid","16956966"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52166"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Physiological Soc"],["dc.relation.issn","0363-6143"],["dc.title","Imaging of respiratory-related population activity with single-cell resolution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","The FASEB Journal"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Dutschmann, Mathias"],["dc.contributor.author","Mueller, M."],["dc.date.accessioned","2018-11-07T10:07:51Z"],["dc.date.available","2018-11-07T10:07:51Z"],["dc.date.issued","2006"],["dc.format.extent","A369"],["dc.identifier.isi","000236206503096"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39357"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Federation Amer Soc Exp Biol"],["dc.publisher.place","Bethesda"],["dc.relation.conference","Experimental Biology 2006 Annual Meeting"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","0892-6638"],["dc.title","Imaging of respiratory population activity in the rat preBotzinger region (PBC) with single cell resolution"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4132"],["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","4148"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","de Meijere, Armin"],["dc.contributor.author","Schimer, H."],["dc.contributor.author","Stein, F."],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Duetsch, Michael"],["dc.contributor.author","Wu, Y."],["dc.contributor.author","Noltemeyer, M."],["dc.contributor.author","Belgardt, T."],["dc.contributor.author","Knieriem, B."],["dc.date.accessioned","2018-11-07T08:46:31Z"],["dc.date.available","2018-11-07T08:46:31Z"],["dc.date.issued","2005"],["dc.description.abstract","A wide range of cyclopenta[b]pyrans 4 has been synthesized in a one-pot reaction by treatment of different 2-donorsubstituted ethenylcarbene-chromium complexes 2 with alkynes in THF in moderate to excellent yields (41-90% for 14 out of 25 examples). The starting materials 2 are readily available in good to excellent yields (76-99% for 25 out of 36 examples) by Michael addition of amines, alcohols and thiols, respectively, to the corresponding alkynylcarbenechromium complexes 1. Due to their 10 pi-electrons in a cross-conjugated bicyclic system, cyclopenta[b]pyrans have been termed pseudoazulenes, as they indeed have similar UV/Vis-spectroscopic properties."],["dc.identifier.doi","10.1002/chem.200500043"],["dc.identifier.isi","000230380300009"],["dc.identifier.pmid","15861477"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20713"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.issn","0947-6539"],["dc.title","An efficient three-step synthesis of cyclopenta[b]pyrans via 2-donor-substituted Fischer ethenylcarbenechromium complexes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S292"],["dc.bibliographiccitation.journal","Journal of Cerebral Blood Flow & Metabolism"],["dc.bibliographiccitation.lastpage","S293"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Mueller, M."],["dc.contributor.author","Kron, Martina"],["dc.contributor.author","Dutschmann, Mathias"],["dc.contributor.author","Funke, Frank"],["dc.date.accessioned","2018-11-07T11:23:50Z"],["dc.date.available","2018-11-07T11:23:50Z"],["dc.date.issued","2009"],["dc.identifier.isi","000270329900354"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56273"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.publisher.place","New york"],["dc.relation.conference","24th International Symposium on Cerebral Blood Flow and Metabolism/9th International Conference on Quantification of Brain Function with PET"],["dc.relation.eventlocation","Chicago, IL"],["dc.relation.issn","0271-678X"],["dc.title","Infant brainstem is prone to the generation of spreading depression during severe hypoxia"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","937"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Pflügers Archiv : European Journal of Physiology"],["dc.bibliographiccitation.lastpage","952"],["dc.bibliographiccitation.volume","458"],["dc.contributor.author","Gerich, Florian J."],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Hildebrandt, Belinda"],["dc.contributor.author","Fasshauer, Martin"],["dc.contributor.author","Müller, Michael"],["dc.date.accessioned","2018-09-28T10:04:34Z"],["dc.date.available","2018-09-28T10:04:34Z"],["dc.date.issued","2009"],["dc.description.abstract","Reactive oxygen species (ROS) released from (dys-)functioning mitochondria contribute to normal and pathophysiological cellular signaling by modulating cytosolic redox state and redox-sensitive proteins. To identify putative redox targets involved in such signaling, we exposed hippocampal neurons to hydrogen peroxide (H(2)O(2)). Redox-sensitive dyes indicated that externally applied H(2)O(2) may oxidize intracellular targets in cell cultures and acute tissue slices. In cultured neurons, H(2)O(2) (EC(50) 118 microM) induced an intracellular Ca(2+) rise which could still be evoked upon Ca(2+) withdrawal and mitochondrial uncoupling. It was, however, antagonized by thapsigargin, dantrolene, 2-aminoethoxydiphenyl borate, and high levels of ryanodine, which identifies the endoplasmic reticulum (ER) as the intracellular Ca(2+) store involved. Intracellular accumulation of endogenously generated H(2)O(2)-provoked by inhibiting glutathione peroxidase-also released Ca(2+) from the ER, as did extracellular generation of superoxide. Phospholipase C (PLC)-mediated metabotropic signaling was depressed in the presence of H(2)O(2), but cytosolic cyclic adenosine-5'-monophosphate (cAMP) levels were not affected. H(2)O(2) (0.2-5 mM) moderately depolarized mitochondria, halted their intracellular trafficking in a Ca(2+)- and cAMP-independent manner, and directly oxidized cellular nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FADH(2)). In part, the mitochondrial depolarization reflects uptake of Ca(2+) previously released from the ER. We conclude that H(2)O(2) releases Ca(2+) from the ER via both ryanodine and inositol trisphosphate receptors. Mitochondrial function is not markedly impaired even by millimolar concentrations of H(2)O(2). Such modulation of Ca(2+) signaling and organelle interaction by ROS affects the efficacy of PLC-mediated metabotropic signaling and may contribute to the adjustment of neuronal function to redox conditions and metabolic supply."],["dc.identifier.doi","10.1007/s00424-009-0672-0"],["dc.identifier.pmid","19430810"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15851"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1432-2013"],["dc.title","H(2)O(2)-mediated modulation of cytosolic signaling and organelle function in rat hippocampus"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]