Schulte, Reiner

[["dc.bibliographiccitation.firstpage","300"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Virology"],["dc.bibliographiccitation.lastpage","309"],["dc.bibliographiccitation.volume","383"],["dc.contributor.author","Schulte, Reiner"],["dc.contributor.author","Suh, You-Suk"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Ochieng, Washingtone"],["dc.contributor.author","Sopper, Sieghart"],["dc.contributor.author","Kim, Kwang S."],["dc.contributor.author","Ahn, So-Shin"],["dc.contributor.author","Park, Ki S."],["dc.contributor.author","Stolte-Leeb, Nicole"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.date.accessioned","2021-06-01T10:49:57Z"],["dc.date.available","2021-06-01T10:49:57Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1016/j.virol.2008.10.012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86471"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0042-6822"],["dc.title","Mucosal prior to systemic application of recombinant adenovirus boosting is more immunogenic than systemic application twice but confers similar protection against SIV-challenge in DNA vaccine-primed macaques"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","463"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","HYBRIDOMA"],["dc.bibliographiccitation.lastpage","472"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Mueller, Daniel A."],["dc.contributor.author","Heinig, Lars"],["dc.contributor.author","Ramljak, Sanja"],["dc.contributor.author","Krueger, Astrid"],["dc.contributor.author","Schulte, Reiner"],["dc.contributor.author","Wrede, Arne"],["dc.contributor.author","Stuke, Andreas W."],["dc.date.accessioned","2018-11-07T08:36:15Z"],["dc.date.available","2018-11-07T08:36:15Z"],["dc.date.issued","2010"],["dc.description.abstract","Because of their high antigen specificity and metabolic stability, genetically engineered human monoclonal antibodies are on the way to becoming one of the most promising medical diagnostics and therapeutics. In order to establish an in vitro system capable of producing such biosimilar antibodies, we used human constant chain sequences to design the novel human antibody expressing vector cassette pMAB-ABX. A bidirectional tetracycline (tet)-controllable promotor was used for harmonized expression of immunoglobulin type G (IgG) heavy and light chains. As an example we used anti-prion protein (anti-PrP) IgGs. Therefore, the variable heavy (V(H)) and light chain (V(L)) sequences of anti-PrP antibodies, previously generated in our laboratory by DNA immunization of prion protein knock-out mice, were isolated from murine hybridoma cell lines and inserted into pMAB-ABX vector. After transfection of Chinese hamster ovary (CHO) cells, a number of stable antibody producing cell clones were selected. One cell line (pMAB-ABX-13F10/3B5) stably expressing the recombinant humanized antibody (rechuAb) 13F10/3B5 was selected for detailed characterization by Western blot, immunofluorescence, and flow cytometric analyses. The full-length recombinant humanized IgG antibody showed a high level of expression in the cytoplasm. In conclusion, the new cell system described here is a suitable tool to produce functional intact full-length humanized IgG antibodies."],["dc.description.sponsorship","DPZ; Ersatzmethoden zum Tierversuch; German BMBF [FKZ 0315279A]"],["dc.identifier.doi","10.1089/hyb.2010.0041"],["dc.identifier.isi","000285145300001"],["dc.identifier.pmid","21087094"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18267"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mary Ann Liebert Inc"],["dc.relation.issn","1554-0014"],["dc.title","Conditional Expression of Full-Length Humanized Anti-Prion Protein Antibodies in Chinese Hamster Ovary Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Retrovirology"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Schultheiss, Tina"],["dc.contributor.author","Schulte, Reiner"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Ibing, Wiebke"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.date.accessioned","2021-06-01T10:48:00Z"],["dc.date.available","2021-06-01T10:48:00Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1186/1742-4690-8-24"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85796"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1742-4690"],["dc.title","Strong mucosal immune responses in SIV infected macaques contribute to viral control and preserved CD4+ T-cell levels in blood and mucosal tissues"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","162"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Acquired Immune Deficiency Syndromes"],["dc.bibliographiccitation.lastpage","169"],["dc.bibliographiccitation.volume","52"],["dc.contributor.author","Ochieng, Washingtone"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Schulte, Reiner"],["dc.contributor.author","Suh, You-Suk"],["dc.contributor.author","Kim, Kwang Soon"],["dc.contributor.author","Sung, Young C."],["dc.contributor.author","Hunsmann, Gerhard"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Sopper, Sieghart"],["dc.date.accessioned","2021-06-01T10:47:01Z"],["dc.date.available","2021-06-01T10:47:01Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1097/QAI.0b013e3181b22f4a"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85451"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","1525-4135"],["dc.title","Susceptibility to Simian Immunodeficiency Virus Ex Vivo Predicts Outcome of a Prime-Boost Vaccine After SIVmac239 Challenge"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3997"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of Virology"],["dc.bibliographiccitation.lastpage","4007"],["dc.bibliographiccitation.volume","88"],["dc.contributor.author","Grunwald, Thomas"],["dc.contributor.author","Tenbusch, Matthias"],["dc.contributor.author","Schulte, Reiner"],["dc.contributor.author","Raue, Katharina"],["dc.contributor.author","Wolf, Hans"],["dc.contributor.author","Hannaman, Drew"],["dc.contributor.author","de Swart, Rik L."],["dc.contributor.author","Überla, Klaus"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.editor","Lyles, D. S."],["dc.date.accessioned","2022-10-06T13:25:38Z"],["dc.date.available","2022-10-06T13:25:38Z"],["dc.date.issued","2014"],["dc.description.abstract","ABSTRACT\n \n Induction of long-lasting immunity against viral respiratory tract infections remains an elusive goal. Using a nonhuman primate model of human respiratory syncytial virus (hRSV) infection, we compared mucosal and systemic immune responses induced by different DNA delivery approaches to a novel parenteral DNA prime-tonsillar adenoviral vector booster immunization regimen. Intramuscular (i.m.) electroporation (EP) of a DNA vaccine encoding the fusion protein of hRSV induced stronger systemic immune responses than intradermal EP, tattoo immunization, and conventional i.m. DNA injection. A single EP i.m., followed by two atraumatic tonsillar immunizations with the adenoviral vector, elicited strong systemic immune responses, an unique persistent CD4\n +\n and CD8\n +\n T cell response in the lower respiratory tract and protection from intranasal hRSV challenge. Thus, parenteral DNA priming followed by booster immunization targeted to a mucosal inductive site constitutes an effective vaccine regimen for eliciting protective immune responses at mucosal effector sites.\n \n \n IMPORTANCE\n The human respiratory syncytial virus (hRSV) is the most common cause of severe respiratory tract disease in infancy and leads to substantial morbidity and morality in the elderly. In this study, we compared the immunogenicity and efficacy of several gene-based immunization protocols in rhesus macaques. Thereby, we found that the combination of an initially parenterally delivered DNA vaccine with a subsequent atraumatic tonsillar adenoviral vector immunization results in a strong systemic immune response accompanied by an exceptional high T-cell response in the mucosa. Strikingly, these animals were protected against a RSV challenge infection controlling the viral replication indicated by a 1,000-fold-lower viral load in the lower respiratory tract. Since mucosal cellular responses of this strength had not been described in earlier RSV vaccine studies, this heterologous DNA prime-tonsillar boost vaccine strategy is very promising and should be pursued for further preclinical and clinical testing."],["dc.identifier.doi","10.1128/JVI.02736-13"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114883"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1098-5514"],["dc.relation.issn","0022-538X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://journals.asm.org/non-commercial-tdm-license"],["dc.title","Novel Vaccine Regimen Elicits Strong Airway Immune Responses and Control of Respiratory Syncytial Virus in Nonhuman Primates"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6690"],["dc.bibliographiccitation.issue","51"],["dc.bibliographiccitation.journal","Vaccine"],["dc.bibliographiccitation.lastpage","6698"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Suh, You S."],["dc.contributor.author","Park, Ki S."],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Kim, Kwang S."],["dc.contributor.author","Ahn, So S."],["dc.contributor.author","Franz, Monika"],["dc.contributor.author","Schulte, Reiner"],["dc.contributor.author","Wilfingseder, Doris"],["dc.contributor.author","Stoiber, Heribert"],["dc.contributor.author","Überla, Klaus"],["dc.contributor.author","Sung, Young C."],["dc.date.accessioned","2022-10-06T13:33:27Z"],["dc.date.available","2022-10-06T13:33:27Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1016/j.vaccine.2008.07.055"],["dc.identifier.pii","S0264410X08010098"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115633"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","0264-410X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Prolonged survival of vaccinated macaques after oral SIVmac239 challenge regardless of viremia control in the chronic phase"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","195"],["dc.bibliographiccitation.issue","4-5"],["dc.bibliographiccitation.journal","Journal of Medical Primatology"],["dc.bibliographiccitation.lastpage","205"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Suh, You Suk"],["dc.contributor.author","Park, Ki Seok"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Kim, Kwang Soon"],["dc.contributor.author","Ahn, Soshin"],["dc.contributor.author","Franz, Monika"],["dc.contributor.author","Schulte, Reiner"],["dc.contributor.author","Stolte-Leeb, Nicole"],["dc.contributor.author","Hunsmann, Gerhard"],["dc.contributor.author","Sung, Young Chul"],["dc.date.accessioned","2022-10-06T13:25:15Z"],["dc.date.available","2022-10-06T13:25:15Z"],["dc.date.issued","2007"],["dc.identifier.doi","10.1111/j.1600-0684.2007.00237.x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114799"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1600-0684"],["dc.relation.issn","0047-2565"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Immunogenicity of a DNA prime and recombinant adenovirus boost regime significantly varies between rhesus macaques of Chinese and Indian origins"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]