Markus, Marietta Andrea

[["dc.bibliographiccitation.firstpage","70"],["dc.bibliographiccitation.journal","NeuroImage"],["dc.bibliographiccitation.lastpage","80"],["dc.bibliographiccitation.volume","199"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-04T13:32:26Z"],["dc.date.available","2020-03-04T13:32:26Z"],["dc.date.issued","2019"],["dc.description.abstract","Knowledge of the three-dimensional (3d) neuronal cytoarchitecture is an important factor in order to understand the connection between tissue structure and function or to visualize pathological changes in neurodegenerative diseases or tumor development. The gold standard in neuropathology is histology, a technique which provides insights into the cellular organization based on sectioning of the sample. Conventional histology, however, misses the complete 3d information as only individual two-dimensional slices through the object are available. In this work, we use propagation-based phase-contrast x-ray tomography to perform 3d virtual histology on cerebellar tissue from mice. This technique enables us to non-invasively visualize the entire 3d density distribution of the examined samples at isotropic (sub-)cellular resolution. One central challenge, however, of the technique is the fact that contrast for important structural features can be easily lost due to small electron density differences, notably between the cells and surrounding tissue. Here, we evaluate the influence of different embedding media, which are intermediate steps in sample preparation for classical histology, on contrast formation and examine the applicability of the different sample preparations both at a synchrotron-based holotomography setup as well as a laboratory source."],["dc.identifier.doi","10.1016/j.neuroimage.2019.05.043"],["dc.identifier.pmid","31129306"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16568"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63105"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/201"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1095-9572"],["dc.relation.issn","1053-8119"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Alves (Translationale Molekulare Bildgebung)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Contrast enhancement for visualizing neuronal cytoarchitecture by propagation-based x-ray phase-contrast tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","143"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","155"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","dal Monego, Simeone"],["dc.contributor.author","Larsson, Emanuel"],["dc.contributor.author","Mohammadi, Sara"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Garrovo, Chiara"],["dc.contributor.author","Biffi, Stefania"],["dc.contributor.author","Lorenzon, Andrea"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Accardo, Agostino"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Tromba, Giuliana"],["dc.date.accessioned","2017-09-07T11:44:46Z"],["dc.date.available","2017-09-07T11:44:46Z"],["dc.date.issued","2015"],["dc.description.abstract","Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24 h, lungs were kept in situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline (Elettra Synchrotron Light Source, Italy). Single-distance phase retrieval was used to generate data sets with ten times greater contrast-to-noise ratio than absorption-based CT (in our setup), thus allowing to depict and quantify structural hallmarks of asthmatic lungs such as reduced air volume, obstruction of airways and increased soft-tissue content. Furthermore, we found a higher concentration as well as a specific accumulation of the barium-labelled macrophages in asthmatic lung tissue. It is believe that XPCT will be beneficial in preclinical asthma research for both the assessment of therapeutic response as well as the analysis of the role of the recruitment of macrophages to inflammatory sites."],["dc.identifier.doi","10.1107/S1600577514021730"],["dc.identifier.fs","608140"],["dc.identifier.gro","3141991"],["dc.identifier.isi","000346850200022"],["dc.identifier.pmid","25537601"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11558"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3334"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/230739/EU//P3AGI"],["dc.relation.eissn","1600-5775"],["dc.relation.issn","0909-0495"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Functionalized synchrotron in-line phase-contrast computed tomography: a novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","09973"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:44:24Z"],["dc.date.available","2017-09-07T11:44:24Z"],["dc.date.issued","2015"],["dc.description.abstract","We have performed x-ray phase-contrast tomography on mouse lung tissue. Using a divergent x-ray beam generated by nanoscale focusing, we used zoom tomography to produce three-dimensional reconstructions with selectable magnification, resolution, and field of view. Thus, macroscopic tissue samples extending over several mm can be studied in sub-cellular-level structural detail. The zoom capability and, in particular, the high dose efficiency are enabled by the near-perfect exit wavefront of an optimized x-ray waveguide channel. In combination with suitable phase-retrieval algorithms, challenging radiation-sensitive and low-contrast samples can be reconstructed with minimal artefacts. The dose efficiency of the method is demonstrated by the reconstruction of living macrophages both with and without phagocytized contrast agents. We also used zoom tomography to visualize barium-labelled macrophages in the context of morphological structures in asthmatic and healthy mouse lung tissue one day after intratracheal application. The three-dimensional reconstructions showed that the macrophages predominantly localized to the alveoli, but they were also found in bronchial walls, indicating that these cells might be able to migrate from the lumen of the bronchi through the epithelium."],["dc.identifier.doi","10.1038/srep09973"],["dc.identifier.fs","615791"],["dc.identifier.gro","3141904"],["dc.identifier.isi","000354310200001"],["dc.identifier.pmid","25966338"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13625"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2367"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Cell Line, Transformed"],["dc.subject.mesh","Cell Movement"],["dc.subject.mesh","Macrophages, Alveolar"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Pulmonary Alveoli"],["dc.subject.mesh","Respiratory Mucosa"],["dc.subject.mesh","Tomography, X-Ray"],["dc.title","Phase-contrast zoom tomography reveals precise locations of macrophages in mouse lungs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]