Dresbach, Thomas

[["dc.bibliographiccitation.firstpage","11116"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.lastpage","11121"],["dc.bibliographiccitation.volume","107"],["dc.contributor.author","Stan, A."],["dc.contributor.author","Pielarski, K. N."],["dc.contributor.author","Brigadski, T."],["dc.contributor.author","Wittenmayer, N."],["dc.contributor.author","Fedorchenko, O."],["dc.contributor.author","Gohla, A."],["dc.contributor.author","Lessmann, V."],["dc.contributor.author","Dresbach, T."],["dc.contributor.author","Gottmann, K."],["dc.date.accessioned","2019-07-09T11:52:58Z"],["dc.date.available","2019-07-09T11:52:58Z"],["dc.date.issued","2010"],["dc.description.abstract","Cell adhesion molecules are key players in transsynaptic communication, precisely coordinating presynaptic differentiation with postsynaptic specialization. At glutamatergic synapses, their retrograde signaling has been proposed to control presynaptic vesicle clustering at active zones. However, how the different types of cell adhesion molecules act together during this decisive step of synapse maturation is largely unexplored. Using a knockout approach, we show that two synaptic adhesion systems, N-cadherin and neuroligin-1, cooperate to control vesicle clustering at nascent synapses. Live cell imaging and fluorescence recovery after photobleaching experiments at individual synaptic boutons revealed a strong impairment of vesicle accumulation in the absence of N-cadherin, whereas the formation of active zones was largely unaffected. Strikingly, also the clustering of synaptic vesicles triggered by neuroligin-1 overexpression required the presence of N-cadherin in cultured neurons. Mechanistically, we found that N-cadherin acts by postsynaptically accumulating neuroligin-1 and activating its function via the scaffolding molecule S-SCAM, leading, in turn, to presynaptic vesicle clustering. A similar cooperation of N-cadherin and neuroligin-1 was observed in immature CA3 pyramidal neurons in an organotypic hippocampal network. Moreover, at mature synapses, N-cadherin was required for the increase in release probability and miniature EPSC frequency induced by expressed neuroligin-1. This cooperation of two cell adhesion systems provides a mechanism for coupling bidirectional synapse maturation mediated by neuroligin-1 to cell type recognition processes mediated by classical cadherins."],["dc.identifier.doi","10.1073/pnas.0914233107"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6249"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60310"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Essential cooperation of N-cadherin and neuroligin-1 in the transsynaptic control of vesicle accumulation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","58"],["dc.bibliographiccitation.journal","Frontiers in Neuroanatomy"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Wallrafen, Rebecca"],["dc.contributor.author","Dresbach, Thomas"],["dc.date.accessioned","2019-07-09T11:45:42Z"],["dc.date.available","2019-07-09T11:45:42Z"],["dc.date.issued","2018"],["dc.description.abstract","The assembly and function of presynaptic nerve terminals relies on evolutionarily conserved proteins. A small number of presynaptic proteins occurs only in vertebrates. These proteins may add specialized functions to certain synapses, thus increasing synaptic heterogeneity. Here, we show that the vertebrate-specific synaptic vesicle (SV) protein mover is differentially distributed in the forebrain and cerebellum of the adult mouse. Using a quantitative immunofluorescence approach, we compare the expression of mover to the expression of the general SV marker synaptophysin in 16 brain areas. We find that mover is particularly abundant in the septal nuclei (SNu), ventral pallidum (VPa), amygdala and hippocampus. Within the hippocampus, mover is predominantly associated with excitatory synapses. Its levels are low in layers that receive afferent input from the entorhinal cortex, and high in layers harboring intrahippocampal circuits. In contrast, mover levels are high in all nuclei of the amygdala, and mover is associated with inhibitory synapses in the medioposterior amygdala. Our data reveal a striking heterogeneity in the abundance of mover on three levels, i.e., between brain areas, within individual brain areas and between synapse types. This distribution suggests a role for mover in providing specialization to subsets of synapses, thereby contributing to the functional diversity of brain areas."],["dc.identifier.doi","10.3389/fnana.2018.00058"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15291"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59291"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","The Presynaptic Protein Mover Is Differentially Expressed Across Brain Areas and Synapse Types"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]