Komrakova, Marina

[["dc.bibliographiccitation.firstpage","187"],["dc.bibliographiccitation.journal","Bone"],["dc.bibliographiccitation.lastpage","194"],["dc.bibliographiccitation.volume","64"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Schaefer, Nadine"],["dc.contributor.author","Hallecker, Jan"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.date.accessioned","2018-11-07T09:38:39Z"],["dc.date.available","2018-11-07T09:38:39Z"],["dc.date.issued","2014"],["dc.description.abstract","Current osteoporosis therapies aim to delay bone destruction and have additional anabolic effects. While they have demonstrated some positive effects on bone healing, more progress is needed in this area. This study used the well-known osteoporotic agents estrogen (E) and raloxifene (R) in conjunction with biomechanical whole body vibration (WBV) at a frequency of 70 Hz twice daily for six weeks to stimulate bone healing. Eighty-four 3-month old female Sprague-Dawley rats (12 per group) were bilaterally ovariectomized to develop osteopenia within eight weeks. Osteotomy of the metaphyseal tibiae was performed and fracture healing was then studied using mechanical tests, histomorphometry, computed tomography (mu CT), and gene analysis. We found that E and R improved the structure of osteopenic bones as did WBV alone, although significant levels for WBV were seldom reached. Combination treatments significantly enhanced stiffness (R + WBV; p < 0.05), endosteal bone (R + WBV; p < 0.01), and trabecular density (E + WBV; p < 0.05, R + WBV; p < 0.05). In addition, the expression of osteoclast-specific Trap was significantly reduced after treatment with E, R, or their combination with WBV (p < 0.01). The effects were additive and not inhibitory, leading us to conclude that the combined applications of WBV with E or R may improve the healing of osteopenic bones. The therapies studied are all currently approved for human use, suggesting ready applicability to clinical practice. To better understand the effects of WBV on osteopenic bones, the ideal vibration regime will require further study. (C) 2014 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","German Research Foundation (DFG) [STU 478/3-1]"],["dc.identifier.doi","10.1016/j.bone.2014.04.008"],["dc.identifier.isi","337011500026"],["dc.identifier.pmid","24735975"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33112"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","1873-2763"],["dc.relation.issn","8756-3282"],["dc.title","Whole body vibration during fracture healing intensifies the effects of estradiol and raloxifene in estrogen-deficient rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","408"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Calcified Tissue International"],["dc.bibliographiccitation.lastpage","422"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Nuehnen, V."],["dc.contributor.author","Stueber, H."],["dc.contributor.author","Wassmann, M."],["dc.contributor.author","Wicke, Michael"],["dc.contributor.author","Tezval, Mohammed"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.contributor.author","Sehmisch, Stefan"],["dc.date.accessioned","2018-11-07T10:08:11Z"],["dc.date.available","2018-11-07T10:08:11Z"],["dc.date.issued","2016"],["dc.description.abstract","The aim of the present study was to study the effect of combined therapy of teriparatide (PTH) or strontium ranelate (SR) with whole-body vibration (WBV) on bone healing and muscle properties in an osteopenic rat model. Seventy-two rats (3 months old) were bilaterally ovariectomized (Ovx), and 12 rats were left intact (Non-Ovx). After 8 weeks, bilateral transverse osteotomy was performed at the tibia metaphysis in all rats. Thereafter, Ovx rats were divided into six groups (n = 12): (1) Ovx-no treatment, (2) Ovx + vibration (Vib), (3) SR, (4) SR + Vib, (5) PTH, and (6) PTH + Vib. PTH (40 mu g/kg BW sc. 5x/week) and SR (613 mg/kg BW in food daily) were applied on the day of ovariectomy, vibration treatments 5 days later (vertical, 70 Hz, 0.5 mm, 2x/day for 15 min) for up to 6 weeks. In the WBV + SR group, the callus density, trabecular number, and Alp and Oc gene expression were decreased compared to SR alone. In the WBV + PTH group, the cortical and callus widths, biomechanical properties, Opg gene expression, and Opg/Rankl ratio were increased; the cortical and callus densities were decreased compared to PTH alone. A case of non-bridging was found in both vibrated groups. Vibration alone did not change the bone parameters; PTH possessed a stronger effect than SR therapy. In muscles, combined therapies improved the fiber size of Ovx rats. WBV could be applied alone or in combination with anti-osteoporosis drug therapy to improve muscle tissue. However, in patients with fractures, anti-osteoporosis treatments and the application of vibration could have an adverse effect on bone healing."],["dc.description.sponsorship","German Research Foundation (DFG) [SE 1966/5-1]"],["dc.identifier.doi","10.1007/s00223-016-0156-0"],["dc.identifier.isi","000382633200009"],["dc.identifier.pmid","27272029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39422"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-0827"],["dc.relation.issn","0171-967X"],["dc.title","The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","6893137"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Journal of Nutrition and Metabolism"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","2016"],["dc.contributor.author","Hoffmann, Daniel B."],["dc.contributor.author","Griesel, Markus H."],["dc.contributor.author","Brockhusen, Bastian"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Menger, Bjoern"],["dc.contributor.author","Wassmann, Marco"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Sehmisch, Stephan"],["dc.date.accessioned","2019-07-09T11:42:06Z"],["dc.date.available","2019-07-09T11:42:06Z"],["dc.date.issued","2016"],["dc.description.abstract","Background. 8-Prenylnaringenin (8-PN) is the phytoestrogen with the highest affinity for estrogen receptor-𝛼� (ER-𝛼�), which is required to maintain BMD. The osteoprotective properties of 8-PN have been demonstrated previously in tibiae. We used a rat osteopenia model to perform the first investigation of 8-PN with whole-body vertical vibration (WBVV). Study Design. Ovariectomy was performed on 52 of 64 Sprague-Dawley rats. Five weeks after ovariectomy, one group received daily injections (sc) of 8-PN (1.77mg/kg) for 10 weeks; a second group was treated with both 8-PN and WBVV (twice a day, 15 min, 35Hz, amplitude 0.47 mm). Other groups received either onlyWBVV or no treatment. Methods.The rats were sacrificed 15 weeks after ovariectomy. Lumbar vertebrae and femora were removed for biomechanical and morphological assessment. Results. 8-PN at a cancer-safe dose did not cause fundamental improvements in osteoporotic bones. Treatmentwith 8-PN caused a slight increase in uterine wetweight. Combined therapy using WBVV and 8-PN showed no significant improvements in bone structure and biomechanical properties. Conclusion. We cannot confirm the osteoprotective effects of 8-PN at a cancer-safe dose in primary affected osteoporotic bones. Higher concentrations of 8-PN are not advisable for safety reasons. Adjunctive therapy with WBVV demonstrates no convincing effects on bones."],["dc.identifier.doi","10.1155/2016/6893137"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12873"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58593"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2090-0732"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Effects of 8-Prenylnaringenin and Whole-Body Vibration Therapy on a Rat Model of Osteopenia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","253"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Endocrinology"],["dc.bibliographiccitation.lastpage","262"],["dc.bibliographiccitation.volume","201"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Werner, Carsten"],["dc.contributor.author","Wicke, Michael"],["dc.contributor.author","Nguyen, Ba Tiep"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.date.accessioned","2018-11-07T08:30:21Z"],["dc.date.available","2018-11-07T08:30:21Z"],["dc.date.issued","2009"],["dc.description.abstract","The effect of daidzein (D), 4-methylbenzylidene camphor (4-MBC) or estradiol-17 beta-benzoate (E-2) on muscle of osteoporotic rats during fracture healing was studied. After performing a metaphyseal tibia osteotomy in 96 osteoporotic 5-month-old female Sprague-Dawley rats, they received daily 50 mg D, 200 mg 4-MBC or 0.4 mg E-2 per kg body weight, or soy free (SF) diet up to 36 and 72 days. Mitochondrial activity, fiber area, and capillary density were analyzed in M. gastrocnemius. Osseous callus bridging of fracture was observed in half of the rats after 36 days. By day 72, fracture was healed in most of the animals. State 3 mitochondrial respiration significantly enhanced in E-2, 4-MBC and D groups versus SF after 36 days (30, 32 and 32 vs 23 pmol O-2/s per mg). It declined after 72 days, however, in E-2 group it was still at a higher level versus SF (25, 23 and 21 vs 20 pmol O-2/s per mg). Size of fast oxidative glycolytic (FOG) and fast glycolytic (FG) fibers, capillary density did not differ significantly between the groups, however, at day 36 an increase in D and 4-MBC groups was detectable. FOG diameter was 64, 66, 68, and 58 mu m and FG diameter was 88, 98, 95, and 89 mu m in SF, D, 4-MBC, and E-2 groups. The ratio of capillaries to muscle fiber was 1.1, 1.4, 1.3, and 1.1 in SF, D, 4-MBC and E-2 groups by day 36. D and 4-MBC react similar to estrogen thereby improving oxidative cell metabolism in severe osteoporotic rats. The level of mitochondrial activity was higher, though no significant morphological differences could be shown. Journal of Endocrinology (2009) 201, 253-262"],["dc.description.sponsorship","German Research Society [DFG STU 478]"],["dc.identifier.doi","10.1677/JOE-08-0521"],["dc.identifier.isi","000272629100008"],["dc.identifier.pmid","19273502"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16878"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Bioscientifica Ltd"],["dc.relation.issn","1479-6805"],["dc.relation.issn","0022-0795"],["dc.title","Effect of daidzein, 4-methylbenzylidene camphor or estrogen on gastrocnemius muscle of osteoporotic rats undergoing tibia healing period"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","172"],["dc.bibliographiccitation.journal","Bone Reports"],["dc.bibliographiccitation.lastpage","180"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Stuermer, Ewa"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Schmelz, Ulrich"],["dc.contributor.author","Doell, Carolin"],["dc.contributor.author","Durkaya-Burchhardt, Nuray"],["dc.contributor.author","Fuerst, Benedikt"],["dc.contributor.author","Genotte, Tim"],["dc.contributor.author","Sehmisch, Stephan"],["dc.date.accessioned","2018-10-10T07:53:11Z"],["dc.date.available","2018-10-10T07:53:11Z"],["dc.date.issued","2017"],["dc.description.abstract","While whole-body vibration (WBV) has recently been introduced as a non-pharmacological therapy for osteoporosis, studies have shown that it has no significant effect on the lumbar spine in older women. However, the vibration protocols differed among studies, and the major factor influencing the outcomes is unclear. The intention of the present study was to evaluate the effect of WBV—vertical (v) or horizontal (h) and of different frequencies and application regimes (1 × or 2 ×/d)—on lumbar spine properties in ovariectomized rats (Ovx). Three experiments were conducted. Thirteen-week old female Sprague–Dawley rats were Ovx or left intact (Non-Ovx). After eight weeks, all of the rats underwent metaphyseal osteotomy of the tibiae. Five days later, the rats were divided into six groups (n = 15): 1) intact, 2) Ovx, and 3–6) Ovx exposed to WBV. In Experiment 1, groups 3–6 underwent 35 Hz-v, 50 Hz-v, 70 Hz-v, and 90 Hz-v, respectively. In Experiment 2, groups 3–6 underwent 30 Hz-h, 50 Hz-h, 70 Hz-h, and 90 Hz-h, respectively. In Experiment 3, groups 3–6 underwent 35 Hz-v, 70 Hz-v, 35 Hz-h, and 70 Hz-h, respectively. Vibration exposure was 15 min 1 ×/d in Experiment 1 and 2 and 2 ×/d in Experiment 3 for up to 30 days. Vertebral bodies were used in micro-computed tomography, biomechanical, ashing, and gene expression analyses. Vertical vibrations applied once a day favorably affected bone volume fraction (BV/TV) and Ca2 +/PO43 − and decreased Rankl gene expression. When applied twice a day, v-vibrations diminished mineral content. Horizontal vibrations (1 ×/d) reduced Ca2 +/PO43 − ratio and Opg mRNA level, whereas h-vibration (2 ×/d) normalized OC serum levels. Many of the other measured parameters did not reveal any significant differences between the vibrated groups and the untreated Ovx group. The effect of ovariectomy was confirmed by atrophied uterus, impaired biomechanical properties, and bone mineral density and BV/TV of the vertebral body. The findings of the present study indicate that application frequency rate and direction of vibration might influence spine response differently. However, we were unable to find any clearly beneficial or harmful effect of vibration regimes on the osteopenic lumbar spine in rats."],["dc.fs.pkfprnr","76571"],["dc.identifier.doi","10.1016/j.bonr.2014.12.001"],["dc.identifier.fs","633524"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15922"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Evaluation of twelve vibration regimes applied to improve spine properties in ovariectomized rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","31"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Bone and Mineral Metabolism"],["dc.bibliographiccitation.lastpage","39"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Sehmisch, S."],["dc.contributor.author","Hofmann, A. M."],["dc.contributor.author","Eimer, C."],["dc.contributor.author","Komrakova, M."],["dc.contributor.author","Saul, D."],["dc.contributor.author","Wassmann, M."],["dc.contributor.author","Stürmer, K. M."],["dc.contributor.author","Tezval, M."],["dc.date.accessioned","2018-10-08T06:30:06Z"],["dc.date.accessioned","2020-06-15T06:44:32Z"],["dc.date.available","2018-10-08T06:30:06Z"],["dc.date.available","2020-06-15T06:44:32Z"],["dc.date.issued","2016"],["dc.description.abstract","We investigated the combinatorial effects of whole-body vertical vibration (WBVV) with the primarily osteoanabolic parathyroid hormone (PTH) and the mainly antiresorptive strontium ranelate (SR) in a rat model of osteoporosis. Ovariectomies were performed on 76 three-month-old Sprague-Dawley rats (OVX, n = 76; NON-OVX, n = 12). After 8 weeks, the ovariectomized rats were divided into 6 groups. One group (OVX + PTH) received daily injections of PTH (40 µg/kg body weight/day) for 6 weeks. Another group (OVX + SR) was fed SR-supplemented chow (600 mg/kg body weight/day). Three groups (OVX + VIB, OVX + PTH + VIB, and OVX + SR + VIB) were treated with WBVV twice a day at 70 Hz for 15 min. Two groups (OVX + PTH + VIB, OVX + SR + VIB) were treated additionally with PTH and SR, respectively. The rats were killed at 14 weeks post-ovariectomy. The lumbar vertebrae and femora were removed for biomechanical and morphological assessment. PTH produced statistically significant improvements in biomechanical and structural properties, including bone mineral density (BMD) and trabecular bone quality. In contrast, SR treatment exerted mild effects, with significant effects in cortical thickness only. SR produced no significant improvement in biomechanical properties. WBVV as a single or an adjunctive therapy produced no significant improvements. In conclusion, vibration therapy administered as a single or dual treatment had no significant impact on bones affected by osteoporosis. PTH considerably improved bone quality in osteoporosis cases and is superior to treatment with SR."],["dc.identifier.doi","10.1007/s00774-016-0736-0"],["dc.identifier.pmid","26825660"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66239"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1435-5604"],["dc.title","Comparison of parathyroid hormone and strontium ranelate in combination with whole-body vibration in a rat model of osteoporosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","168"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Calcified Tissue International"],["dc.bibliographiccitation.lastpage","180"],["dc.bibliographiccitation.volume","87"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Werner, Carsten"],["dc.contributor.author","Wicke, Michael"],["dc.contributor.author","Kolios, Leila"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Utesch, Clara"],["dc.contributor.author","Mangal, Orzala"],["dc.contributor.author","Zimmer, Sebastian"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.date.accessioned","2018-11-07T08:40:40Z"],["dc.date.available","2018-11-07T08:40:40Z"],["dc.date.issued","2010"],["dc.description.abstract","This study investigated the effect of vibration on bone healing and muscle in intact and ovariectomized rats. Thirty ovariectomized (at 3 months of age) and 30 intact 5-month old female Sprague-Dawley rats underwent bilateral metaphyseal osteotomy of tibia. Five days later, half of the ovariectomized and of the intact rats were exposed to whole-body vertical vibration (90 Hz, 0.5 mm, 4 x g acceleration) for 15 min twice a day during 30 days. The other animals did not undergo vibration. After decapitation of rats, one tibia was used for computed tomographic, biomechanical, and histological analyses; the other was used for gene expression analyses of alkaline phosphatase (Alp), osteocalcin (Oc), tartrate-resistant acid phosphatase 1, and insulinlike growth factor 1. Serum Alp and Oc were measured. Mitochondrial activity, fiber area and distribution, and capillary densities were analyzed in M. gastrocnemius and M. longissimus. We found that vibration had no effect on body weight and food intake, but it improved cortical and callus densities (97 vs. 99%, 72 vs. 81%), trabecular structure (9 vs. 14 trabecular nodes), blood supply (1.7 vs. 2.1 capillaries/fiber), and oxidative metabolism (17 vs. 23 pmol O(2)/s/mg) in ovariectomized rats. Vibration generally increased muscle fiber size. Tibia biomechanical properties were diminished after vibration. Oc gene expression was higher in vibrated rats. Serum Alp was increased in ovariectomized rats. In ovariectomized rats, vibration resulted in an earlier bridging; in intact rats, callus bridging occurred later after vibration. The chosen vibration regimen (90 Hz, 0.5 mm, 4 x g acceleration, 15 min twice a day) was effective in improving musculoskeletal tissues in ovariectomized rats but was not optimal for fracture healing."],["dc.description.sponsorship","German Research Foundation (DFG) [STU 478/3-1]"],["dc.identifier.doi","10.1007/s00223-010-9381-0"],["dc.identifier.isi","000280062200008"],["dc.identifier.pmid","20532877"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4972"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19283"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0171-967X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Musculoskeletal Response to Whole-Body Vibration During Fracture Healing in Intact and Ovariectomized Rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","20"],["dc.bibliographiccitation.journal","The Open Bone Journal"],["dc.bibliographiccitation.lastpage","26"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Stuermer, Ewa K."],["dc.contributor.author","Sturm, Armin"],["dc.contributor.author","Schmelz, Ulrich"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Stuermer, Klaus M."],["dc.contributor.author","Sehmisch, Stephan"],["dc.date.accessioned","2019-07-09T11:53:38Z"],["dc.date.available","2019-07-09T11:53:38Z"],["dc.date.issued","2012"],["dc.description.abstract","Daily application of parathyroid hormone (PTH 1-34) is used for treatment of osteoporosis. It was investigated whether orchiectomy-induced osteoporotic changes in spine can be ameliorated by every 48h administration of PTH in aged male rats. Eight-month-old male Sprague-Dawley rats were sham operated (n=24) or orchiectomized (Orx, n=36) and maintained untreated over 12 weeks. Thereafter, both tibia underwent transverse metaphyseal osteotomy (Komrakova et al. 2011, J Endocrinol; 209:9-19) and rats were divided into 5 groups treated s.c. as follows: 1) sham vehicle; 2) sham PTH every 24h (PTH/24h); 3) Orx vehicle; 4) Orx PTH/24h; 5) Orx PTH every 48h (PTH/48h). PTH dosage was 40 􀀁g/kg BW per injection. After 5 weeks, lumbar vertebral bodies were used in computed tomographical, biomechanical, histomorphological, ashing and gene expression analyses. Cortical and trabecular densities, biomechanical properties, serum osteocalcin level increased significantly after PTH treatments in all groups (yield load, sham: 232+17N, sham PTH/24h: 376+12N, Orx: 239+16N, Orx PTH/24h: 324+31N, Orx PTH/48h: 297+17N). Bone inorganic weight enhanced after daily PTH application in Orx rats. Bone gene expression did not differ (P>0.05) among the groups. Both PTH administration regimes (24h and 48h) improved impaired bone structure in osteopenic rats. Every 48h application was less effective, however, it improved bone properties to the level observed in healthy (sham) rats. Considering limitation of daily treatments known in humans, these results may be useful for further clinical studies."],["dc.identifier.doi","10.2174/1876525401204010020"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7843"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60468"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1876-5254"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Efficiency of 48h vs. 24h Injection of Parathyroid Hormone for Amelioration of Osteopenic Spine Properties in Male Rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","157"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Endocrinology"],["dc.bibliographiccitation.lastpage","168"],["dc.bibliographiccitation.volume","211"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Schmelz, Ulrich"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Grueger, Thomas"],["dc.contributor.author","Wessling, Thomas"],["dc.contributor.author","Klein, Carolin"],["dc.contributor.author","Birth, Miriam"],["dc.contributor.author","Stuermer, Ewa K"],["dc.date.accessioned","2021-06-01T10:48:26Z"],["dc.date.available","2021-06-01T10:48:26Z"],["dc.date.issued","2011"],["dc.description.abstract","The study investigated the influence of 4-methylbenzylidene camphor (4-MBC), daidzein, and estradiol-17β-benzoate (E 2 ) on either intact or osteotomized cancellous bone in ovariectomized (Ovx) rats. Three-month old Ovx rats were fed with soy-free (SF) diet over 8 weeks; thereafter, bilateral transverse metaphyseal osteotomy of tibia was performed and rats were divided into groups: rats fed with SF diet and SF diet supplemented with 4-MBC (200 mg), daidzein (50 mg), or E 2 (0.4 mg) per kilogram body weight. After 5 or 10 weeks, computed tomographical, biomechanical, histological, and ashing analyses were performed in lumbar spine and tibia of 12 rats from each group. 4-MBC and E 2 improved bone parameters in lumbar spine and tibia, were not favorable for osteotomy healing, and decreased serum osteocalcin level. However, daidzein improved bone parameters to a lesser extent and facilitated osteotomy healing. For lumbar spine, the bone mineral density was 338±9, 346±5, 361±6, and 360±5 mg/cm 3 in SF, daidzein, 4-MBC, and E 2 , respectively, after 10 weeks. For tibia, the yield load was 98±5, 114±3, 90±2, and 52±4 N in SF, daidzein, 4-MBC, and E 2 , respectively, after 10 weeks. Serum daidzein was 54±6 ng/ml in daidzein group and equol was not detected. Alp and Igf1 genes were down-regulated in callus after daidzein and E 2 compared with 4-MBC (week 5). The response of bone tissue and serum markers of bone metabolism could be ordered: daidzein<4-MBC

[["dc.bibliographiccitation.artnumber","400"],["dc.bibliographiccitation.journal","Frontiers in Endocrinology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Saul, Dominik"],["dc.contributor.author","Geisberg, Laura Katharina"],["dc.contributor.author","Gehle, Torben"],["dc.contributor.author","Hoffmann, Daniel Bernd"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Komrakova, Marina"],["dc.date.accessioned","2019-07-16T08:32:27Z"],["dc.date.accessioned","2020-06-10T14:24:58Z"],["dc.date.available","2019-07-16T08:32:27Z"],["dc.date.available","2020-06-10T14:24:58Z"],["dc.date.issued","2019"],["dc.description.abstract","Objective: In aging population, postmenopausal osteoporosis and decline of musculoskeletal function, referred to as \"frailty syndrome\" lead to loss of bone and muscle, causing falls, and fall-related injuries. To limit the impact of this portentous duo, simultaneous treatment of both is needed. Urocortin (UCN) has been reported to improve osteoporotic bone properties while its effect on muscle has not been addressed yet. Design and Methods: We aimed to investigate the effect of urocortin in vivo on skeletal muscle structure in osteopenic rats. Sixty Sprague-Dawley rats were divided into five groups: four were ovariectomized (OVX) and one underwent sham operation (SHAM). One ovariectomized group was left untreated (OVX), while one was treated with urocortin s.c. in 3 μg/kg body weight (bw) (OVX+UCN low), one with 30 μg/kg (OVX+UCN high), while one group was treated with estradiol orally (OVX+E: 0.2 mg/kg bw), each for 35 days. Mm. gastrocnemius, longissimus, and soleus were isolated and capillary density as well as diameters of type I and II fibers were measured. In addition, we examined the effect of UCN on tibia using biomechanical, micro-CT and ashing analysis and investigated the blood serum. Results: We demonstrated a positive effect of UCN on M. soleus, in which fiber diameter was positively influenced. The biomechanical and structural parameters of bone were not changed in UCN treated rats. The higher cholesterol, glucose and triglyceride levels in the \"UCN high\" group raise concern about this treatment. Conclusions: Our results portray urocortin as a substance that can be assessed for future therapeutic treatments of estrogen deficiency. New and Noteworthy: Urocortin has a positive effect on M. soleus (diameter). Urocortin raises serum cholesterol and triglyceride levels. Bone tissue was not affected by UCN."],["dc.identifier.doi","10.3389/fendo.2019.00400"],["dc.identifier.isi","WOS:000473058700001"],["dc.identifier.pmid","31293517"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16282"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61553"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66223"],["dc.identifier.url","https://publons.com/publon/28102255/"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-2392"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Changes in Musculoskeletal System and Metabolism in Osteoporotic Rats Treated With Urocortin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]