Komrakova, Marina

[["dc.bibliographiccitation.firstpage","187"],["dc.bibliographiccitation.journal","Bone"],["dc.bibliographiccitation.lastpage","194"],["dc.bibliographiccitation.volume","64"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Schaefer, Nadine"],["dc.contributor.author","Hallecker, Jan"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.date.accessioned","2018-11-07T09:38:39Z"],["dc.date.available","2018-11-07T09:38:39Z"],["dc.date.issued","2014"],["dc.description.abstract","Current osteoporosis therapies aim to delay bone destruction and have additional anabolic effects. While they have demonstrated some positive effects on bone healing, more progress is needed in this area. This study used the well-known osteoporotic agents estrogen (E) and raloxifene (R) in conjunction with biomechanical whole body vibration (WBV) at a frequency of 70 Hz twice daily for six weeks to stimulate bone healing. Eighty-four 3-month old female Sprague-Dawley rats (12 per group) were bilaterally ovariectomized to develop osteopenia within eight weeks. Osteotomy of the metaphyseal tibiae was performed and fracture healing was then studied using mechanical tests, histomorphometry, computed tomography (mu CT), and gene analysis. We found that E and R improved the structure of osteopenic bones as did WBV alone, although significant levels for WBV were seldom reached. Combination treatments significantly enhanced stiffness (R + WBV; p < 0.05), endosteal bone (R + WBV; p < 0.01), and trabecular density (E + WBV; p < 0.05, R + WBV; p < 0.05). In addition, the expression of osteoclast-specific Trap was significantly reduced after treatment with E, R, or their combination with WBV (p < 0.01). The effects were additive and not inhibitory, leading us to conclude that the combined applications of WBV with E or R may improve the healing of osteopenic bones. The therapies studied are all currently approved for human use, suggesting ready applicability to clinical practice. To better understand the effects of WBV on osteopenic bones, the ideal vibration regime will require further study. (C) 2014 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","German Research Foundation (DFG) [STU 478/3-1]"],["dc.identifier.doi","10.1016/j.bone.2014.04.008"],["dc.identifier.isi","337011500026"],["dc.identifier.pmid","24735975"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33112"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","1873-2763"],["dc.relation.issn","8756-3282"],["dc.title","Whole body vibration during fracture healing intensifies the effects of estradiol and raloxifene in estrogen-deficient rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","408"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Calcified Tissue International"],["dc.bibliographiccitation.lastpage","422"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Nuehnen, V."],["dc.contributor.author","Stueber, H."],["dc.contributor.author","Wassmann, M."],["dc.contributor.author","Wicke, Michael"],["dc.contributor.author","Tezval, Mohammed"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.contributor.author","Sehmisch, Stefan"],["dc.date.accessioned","2018-11-07T10:08:11Z"],["dc.date.available","2018-11-07T10:08:11Z"],["dc.date.issued","2016"],["dc.description.abstract","The aim of the present study was to study the effect of combined therapy of teriparatide (PTH) or strontium ranelate (SR) with whole-body vibration (WBV) on bone healing and muscle properties in an osteopenic rat model. Seventy-two rats (3 months old) were bilaterally ovariectomized (Ovx), and 12 rats were left intact (Non-Ovx). After 8 weeks, bilateral transverse osteotomy was performed at the tibia metaphysis in all rats. Thereafter, Ovx rats were divided into six groups (n = 12): (1) Ovx-no treatment, (2) Ovx + vibration (Vib), (3) SR, (4) SR + Vib, (5) PTH, and (6) PTH + Vib. PTH (40 mu g/kg BW sc. 5x/week) and SR (613 mg/kg BW in food daily) were applied on the day of ovariectomy, vibration treatments 5 days later (vertical, 70 Hz, 0.5 mm, 2x/day for 15 min) for up to 6 weeks. In the WBV + SR group, the callus density, trabecular number, and Alp and Oc gene expression were decreased compared to SR alone. In the WBV + PTH group, the cortical and callus widths, biomechanical properties, Opg gene expression, and Opg/Rankl ratio were increased; the cortical and callus densities were decreased compared to PTH alone. A case of non-bridging was found in both vibrated groups. Vibration alone did not change the bone parameters; PTH possessed a stronger effect than SR therapy. In muscles, combined therapies improved the fiber size of Ovx rats. WBV could be applied alone or in combination with anti-osteoporosis drug therapy to improve muscle tissue. However, in patients with fractures, anti-osteoporosis treatments and the application of vibration could have an adverse effect on bone healing."],["dc.description.sponsorship","German Research Foundation (DFG) [SE 1966/5-1]"],["dc.identifier.doi","10.1007/s00223-016-0156-0"],["dc.identifier.isi","000382633200009"],["dc.identifier.pmid","27272029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39422"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-0827"],["dc.relation.issn","0171-967X"],["dc.title","The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Endocrinological Investigation"],["dc.contributor.author","Komrakova, M."],["dc.contributor.author","Büchler, G."],["dc.contributor.author","Böker, K. O."],["dc.contributor.author","Lehmann, W."],["dc.contributor.author","Schilling, A. F."],["dc.contributor.author","Roch, P. J."],["dc.contributor.author","Taudien, S."],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Sehmisch, S."],["dc.date.accessioned","2022-09-01T09:49:26Z"],["dc.date.available","2022-09-01T09:49:26Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n \n Purpose\n Enobosarm (EN), a selective androgen receptor modulator and raloxifene (RAL), a selective estrogen receptor modulator, have been shown to improve bone tissue in osteoporotic males. The present study evaluated the effects of a combination therapy of EN and RAL on bone properties in orchiectomized rats compared to the respective single treatments.\n \n \n Methods\n Eight-month-old male Sprague–Dawley rats were either left intact (Non-Orx) or orchiectomized (Orx). The Orx rats were divided into four groups (n = 15 each): 1) Orx, 2) EN treatment (Orx + EN), 3) RAL treatment (Orx + RAL), 4) combined treatment (Orx + EN + RAL). EN and RAL (0.4 mg and 7 mg/kg body weight/day) were applied immediately after Orx with a soy-free pelleted diet for up to 18 weeks. The lumbar spine and femora were examined by micro-CT, biomechanical, histomorphological, ashing, and gene expression analyses.\n \n \n Results\n EN exhibited an anabolic effect on bone, improving some of its parameters in Orx rats, but did not affect biomechanical properties. RAL exhibited antiresorptive activity, maintaining the biomechanical and trabecular parameters of Orx rats at the levels of Non-Orx rats. EN + RAL exerted a stronger effect than the single treatments, improving most of the bone parameters. Liver weight increased after all treatments; the kidney, prostate, and levator ani muscle weights increased after EN and EN + RAL treatments. BW was reduced due to a decreased food intake in the Orx + RAL group and due a reduced visceral fat weight in the Orx + EN + RAL group.\n \n \n Conclusion\n The EN + RAL treatment appeared to be promising in preventing male osteoporosis, but given the observed side effects on liver, kidney, and prostate weights, it requires further investigation."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship"," Georg-August-Universität Göttingen 501100003385"],["dc.identifier.doi","10.1007/s40618-022-01865-9"],["dc.identifier.pii","1865"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113421"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","1720-8386"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","A combined treatment with selective androgen and estrogen receptor modulators prevents bone loss in orchiectomized rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","582"],["dc.bibliographiccitation.issue","09"],["dc.bibliographiccitation.journal","Experimental and Clinical Endocrinology & Diabetes"],["dc.bibliographiccitation.lastpage","595"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Blaschke, Martina"],["dc.contributor.author","Ponce, Maria Laura"],["dc.contributor.author","Klüver, Anne"],["dc.contributor.author","Köpp, Regine"],["dc.contributor.author","Hüfner, Michael"],["dc.contributor.author","Schieker, Matthias"],["dc.contributor.author","Miosge, Nicolai"],["dc.contributor.author","Siggelkow, Heide"],["dc.date.accessioned","2021-04-14T08:23:08Z"],["dc.date.available","2021-04-14T08:23:08Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1055/a-1084-3888"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80806"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1439-3646"],["dc.relation.issn","0947-7349"],["dc.title","Decreased Expression of the Human Urea Transporter SLC14A1 in Bone is Induced by Cytokines and Stimulates Adipogenesis of Mesenchymal Progenitor Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","243"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Bone and Mineral Metabolism"],["dc.bibliographiccitation.lastpage","255"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Komrakova, M."],["dc.contributor.author","Pflug, S."],["dc.contributor.author","Oertzen, M. von"],["dc.contributor.author","Saul, D."],["dc.contributor.author","Weiser, L."],["dc.contributor.author","Walde, T. A."],["dc.contributor.author","Wassmann, M."],["dc.contributor.author","Schilling, A. F."],["dc.contributor.author","Lehmann, W."],["dc.contributor.author","Sehmisch, S."],["dc.date.accessioned","2020-06-15T13:52:01Z"],["dc.date.available","2020-06-15T13:52:01Z"],["dc.date.issued","2018"],["dc.description.abstract","We investigated the combinatorial effects of whole-body vertical vibration (WBVV) with the primarily osteoanabolic parathyroid hormone (PTH) and the mainly antiresorptive strontium ranelate (SR) in a rat model of osteoporosis. Ovariectomies were performed on 76 three-month-old Sprague-Dawley rats (OVX, n = 76; NON-OVX, n = 12). After 8 weeks, the ovariectomized rats were divided into 6 groups. One group (OVX + PTH) received daily injections of PTH (40 µg/kg body weight/day) for 6 weeks. Another group (OVX + SR) was fed SR-supplemented chow (600 mg/kg body weight/day). Three groups (OVX + VIB, OVX + PTH + VIB, and OVX + SR + VIB) were treated with WBVV twice a day at 70 Hz for 15 min. Two groups (OVX + PTH + VIB, OVX + SR + VIB) were treated additionally with PTH and SR, respectively. The rats were killed at 14 weeks post-ovariectomy. The lumbar vertebrae and femora were removed for biomechanical and morphological assessment. PTH produced statistically significant improvements in biomechanical and structural properties, including bone mineral density (BMD) and trabecular bone quality. In contrast, SR treatment exerted mild effects, with significant effects in cortical thickness only. SR produced no significant improvement in biomechanical properties. WBVV as a single or an adjunctive therapy produced no significant improvements. In conclusion, vibration therapy administered as a single or dual treatment had no significant impact on bones affected by osteoporosis. PTH considerably improved bone quality in osteoporosis cases and is superior to treatment with SR."],["dc.identifier.doi","10.1007/s00774-018-0929-9"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66263"],["dc.language.iso","en"],["dc.relation.issn","0914-8779"],["dc.title","Evaluation of Ostarine as a Selective Androgen Receptor Modulator in a Rat Model of Postmenopausal Osteoporosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","253"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Endocrinology"],["dc.bibliographiccitation.lastpage","262"],["dc.bibliographiccitation.volume","201"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Werner, Carsten"],["dc.contributor.author","Wicke, Michael"],["dc.contributor.author","Nguyen, Ba Tiep"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.date.accessioned","2018-11-07T08:30:21Z"],["dc.date.available","2018-11-07T08:30:21Z"],["dc.date.issued","2009"],["dc.description.abstract","The effect of daidzein (D), 4-methylbenzylidene camphor (4-MBC) or estradiol-17 beta-benzoate (E-2) on muscle of osteoporotic rats during fracture healing was studied. After performing a metaphyseal tibia osteotomy in 96 osteoporotic 5-month-old female Sprague-Dawley rats, they received daily 50 mg D, 200 mg 4-MBC or 0.4 mg E-2 per kg body weight, or soy free (SF) diet up to 36 and 72 days. Mitochondrial activity, fiber area, and capillary density were analyzed in M. gastrocnemius. Osseous callus bridging of fracture was observed in half of the rats after 36 days. By day 72, fracture was healed in most of the animals. State 3 mitochondrial respiration significantly enhanced in E-2, 4-MBC and D groups versus SF after 36 days (30, 32 and 32 vs 23 pmol O-2/s per mg). It declined after 72 days, however, in E-2 group it was still at a higher level versus SF (25, 23 and 21 vs 20 pmol O-2/s per mg). Size of fast oxidative glycolytic (FOG) and fast glycolytic (FG) fibers, capillary density did not differ significantly between the groups, however, at day 36 an increase in D and 4-MBC groups was detectable. FOG diameter was 64, 66, 68, and 58 mu m and FG diameter was 88, 98, 95, and 89 mu m in SF, D, 4-MBC, and E-2 groups. The ratio of capillaries to muscle fiber was 1.1, 1.4, 1.3, and 1.1 in SF, D, 4-MBC and E-2 groups by day 36. D and 4-MBC react similar to estrogen thereby improving oxidative cell metabolism in severe osteoporotic rats. The level of mitochondrial activity was higher, though no significant morphological differences could be shown. Journal of Endocrinology (2009) 201, 253-262"],["dc.description.sponsorship","German Research Society [DFG STU 478]"],["dc.identifier.doi","10.1677/JOE-08-0521"],["dc.identifier.isi","000272629100008"],["dc.identifier.pmid","19273502"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16878"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Bioscientifica Ltd"],["dc.relation.issn","1479-6805"],["dc.relation.issn","0022-0795"],["dc.title","Effect of daidzein, 4-methylbenzylidene camphor or estrogen on gastrocnemius muscle of osteoporotic rats undergoing tibia healing period"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","172"],["dc.bibliographiccitation.journal","Bone Reports"],["dc.bibliographiccitation.lastpage","180"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Stuermer, Ewa"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Schmelz, Ulrich"],["dc.contributor.author","Doell, Carolin"],["dc.contributor.author","Durkaya-Burchhardt, Nuray"],["dc.contributor.author","Fuerst, Benedikt"],["dc.contributor.author","Genotte, Tim"],["dc.contributor.author","Sehmisch, Stephan"],["dc.date.accessioned","2018-10-10T07:53:11Z"],["dc.date.available","2018-10-10T07:53:11Z"],["dc.date.issued","2017"],["dc.description.abstract","While whole-body vibration (WBV) has recently been introduced as a non-pharmacological therapy for osteoporosis, studies have shown that it has no significant effect on the lumbar spine in older women. However, the vibration protocols differed among studies, and the major factor influencing the outcomes is unclear. The intention of the present study was to evaluate the effect of WBV—vertical (v) or horizontal (h) and of different frequencies and application regimes (1 × or 2 ×/d)—on lumbar spine properties in ovariectomized rats (Ovx). Three experiments were conducted. Thirteen-week old female Sprague–Dawley rats were Ovx or left intact (Non-Ovx). After eight weeks, all of the rats underwent metaphyseal osteotomy of the tibiae. Five days later, the rats were divided into six groups (n = 15): 1) intact, 2) Ovx, and 3–6) Ovx exposed to WBV. In Experiment 1, groups 3–6 underwent 35 Hz-v, 50 Hz-v, 70 Hz-v, and 90 Hz-v, respectively. In Experiment 2, groups 3–6 underwent 30 Hz-h, 50 Hz-h, 70 Hz-h, and 90 Hz-h, respectively. In Experiment 3, groups 3–6 underwent 35 Hz-v, 70 Hz-v, 35 Hz-h, and 70 Hz-h, respectively. Vibration exposure was 15 min 1 ×/d in Experiment 1 and 2 and 2 ×/d in Experiment 3 for up to 30 days. Vertebral bodies were used in micro-computed tomography, biomechanical, ashing, and gene expression analyses. Vertical vibrations applied once a day favorably affected bone volume fraction (BV/TV) and Ca2 +/PO43 − and decreased Rankl gene expression. When applied twice a day, v-vibrations diminished mineral content. Horizontal vibrations (1 ×/d) reduced Ca2 +/PO43 − ratio and Opg mRNA level, whereas h-vibration (2 ×/d) normalized OC serum levels. Many of the other measured parameters did not reveal any significant differences between the vibrated groups and the untreated Ovx group. The effect of ovariectomy was confirmed by atrophied uterus, impaired biomechanical properties, and bone mineral density and BV/TV of the vertebral body. The findings of the present study indicate that application frequency rate and direction of vibration might influence spine response differently. However, we were unable to find any clearly beneficial or harmful effect of vibration regimes on the osteopenic lumbar spine in rats."],["dc.fs.pkfprnr","76571"],["dc.identifier.doi","10.1016/j.bonr.2014.12.001"],["dc.identifier.fs","633524"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15922"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Evaluation of twelve vibration regimes applied to improve spine properties in ovariectomized rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","31"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Bone and Mineral Metabolism"],["dc.bibliographiccitation.lastpage","39"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Hoffmann, D. B."],["dc.contributor.author","Sehmisch, S."],["dc.contributor.author","Hofmann, A. M."],["dc.contributor.author","Eimer, C."],["dc.contributor.author","Komrakova, M."],["dc.contributor.author","Saul, D."],["dc.contributor.author","Wassmann, M."],["dc.contributor.author","Stürmer, K. M."],["dc.contributor.author","Tezval, M."],["dc.date.accessioned","2018-10-08T06:30:06Z"],["dc.date.accessioned","2020-06-15T06:44:32Z"],["dc.date.available","2018-10-08T06:30:06Z"],["dc.date.available","2020-06-15T06:44:32Z"],["dc.date.issued","2016"],["dc.description.abstract","We investigated the combinatorial effects of whole-body vertical vibration (WBVV) with the primarily osteoanabolic parathyroid hormone (PTH) and the mainly antiresorptive strontium ranelate (SR) in a rat model of osteoporosis. Ovariectomies were performed on 76 three-month-old Sprague-Dawley rats (OVX, n = 76; NON-OVX, n = 12). After 8 weeks, the ovariectomized rats were divided into 6 groups. One group (OVX + PTH) received daily injections of PTH (40 µg/kg body weight/day) for 6 weeks. Another group (OVX + SR) was fed SR-supplemented chow (600 mg/kg body weight/day). Three groups (OVX + VIB, OVX + PTH + VIB, and OVX + SR + VIB) were treated with WBVV twice a day at 70 Hz for 15 min. Two groups (OVX + PTH + VIB, OVX + SR + VIB) were treated additionally with PTH and SR, respectively. The rats were killed at 14 weeks post-ovariectomy. The lumbar vertebrae and femora were removed for biomechanical and morphological assessment. PTH produced statistically significant improvements in biomechanical and structural properties, including bone mineral density (BMD) and trabecular bone quality. In contrast, SR treatment exerted mild effects, with significant effects in cortical thickness only. SR produced no significant improvement in biomechanical properties. WBVV as a single or an adjunctive therapy produced no significant improvements. In conclusion, vibration therapy administered as a single or dual treatment had no significant impact on bones affected by osteoporosis. PTH considerably improved bone quality in osteoporosis cases and is superior to treatment with SR."],["dc.identifier.doi","10.1007/s00774-016-0736-0"],["dc.identifier.pmid","26825660"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66239"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1435-5604"],["dc.title","Comparison of parathyroid hormone and strontium ranelate in combination with whole-body vibration in a rat model of osteoporosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","373"],["dc.bibliographiccitation.journal","Aquaculture"],["dc.bibliographiccitation.lastpage","379"],["dc.bibliographiccitation.volume","497"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Knorr, Christoph"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Hoerstgen-Schwark, Gabriele"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2020-12-10T14:22:29Z"],["dc.date.available","2020-12-10T14:22:29Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.aquaculture.2018.08.010"],["dc.identifier.issn","0044-8486"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71634"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Sex discrimination in rainbow trout (Oncorhynchus mykiss) using various sources of DNA and different genetic markers"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","157"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Endocrinology"],["dc.bibliographiccitation.lastpage","168"],["dc.bibliographiccitation.volume","211"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Schmelz, Ulrich"],["dc.contributor.author","Frauendorf, Holm"],["dc.contributor.author","Grueger, Thomas"],["dc.contributor.author","Wessling, Thomas"],["dc.contributor.author","Klein, Carolin"],["dc.contributor.author","Birth, Miriam"],["dc.contributor.author","Stuermer, Ewa K"],["dc.date.accessioned","2021-06-01T10:48:26Z"],["dc.date.available","2021-06-01T10:48:26Z"],["dc.date.issued","2011"],["dc.description.abstract","The study investigated the influence of 4-methylbenzylidene camphor (4-MBC), daidzein, and estradiol-17β-benzoate (E 2 ) on either intact or osteotomized cancellous bone in ovariectomized (Ovx) rats. Three-month old Ovx rats were fed with soy-free (SF) diet over 8 weeks; thereafter, bilateral transverse metaphyseal osteotomy of tibia was performed and rats were divided into groups: rats fed with SF diet and SF diet supplemented with 4-MBC (200 mg), daidzein (50 mg), or E 2 (0.4 mg) per kilogram body weight. After 5 or 10 weeks, computed tomographical, biomechanical, histological, and ashing analyses were performed in lumbar spine and tibia of 12 rats from each group. 4-MBC and E 2 improved bone parameters in lumbar spine and tibia, were not favorable for osteotomy healing, and decreased serum osteocalcin level. However, daidzein improved bone parameters to a lesser extent and facilitated osteotomy healing. For lumbar spine, the bone mineral density was 338±9, 346±5, 361±6, and 360±5 mg/cm 3 in SF, daidzein, 4-MBC, and E 2 , respectively, after 10 weeks. For tibia, the yield load was 98±5, 114±3, 90±2, and 52±4 N in SF, daidzein, 4-MBC, and E 2 , respectively, after 10 weeks. Serum daidzein was 54±6 ng/ml in daidzein group and equol was not detected. Alp and Igf1 genes were down-regulated in callus after daidzein and E 2 compared with 4-MBC (week 5). The response of bone tissue and serum markers of bone metabolism could be ordered: daidzein<4-MBC