Agüi-Gonzalez, Paola

[["dc.bibliographiccitation.artnumber","7129"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Dankovich, Tal M."],["dc.contributor.author","Kaushik, Rahul"],["dc.contributor.author","Olsthoorn, Linda H. M."],["dc.contributor.author","Petersen, Gabriel Cassinelli"],["dc.contributor.author","Giro, Philipp Emanuel"],["dc.contributor.author","Kluever, Verena"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Grewe, Katharina"],["dc.contributor.author","Bao, Guobin"],["dc.contributor.author","Beuermann, Sabine"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.date.accessioned","2022-01-11T14:05:59Z"],["dc.date.available","2022-01-11T14:05:59Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract The brain extracellular matrix (ECM) consists of extremely long-lived proteins that assemble around neurons and synapses, to stabilize them. The ECM is thought to change only rarely, in relation to neuronal plasticity, through ECM proteolysis and renewed protein synthesis. We report here an alternative ECM remodeling mechanism, based on the recycling of ECM molecules. Using multiple ECM labeling and imaging assays, from super-resolution optical imaging to nanoscale secondary ion mass spectrometry, both in culture and in brain slices, we find that a key ECM protein, Tenascin-R, is frequently endocytosed, and later resurfaces, preferentially near synapses. The TNR molecules complete this cycle within ~3 days, in an activity-dependent fashion. Interfering with the recycling process perturbs severely neuronal function, strongly reducing synaptic vesicle exo- and endocytosis. We conclude that the neuronal ECM can be remodeled frequently through mechanisms that involve endocytosis and recycling of ECM proteins."],["dc.identifier.doi","10.1038/s41467-021-27462-7"],["dc.identifier.pii","27462"],["dc.identifier.pmid","34880248"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97795"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/371"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/163"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/145"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P09: Proteinsortierung in der Synapse: Prinzipien und molekulare Organisation"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.eissn","2041-1723"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 4.0"],["dc.title","Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1542"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","ACS Chemical Neuroscience"],["dc.bibliographiccitation.lastpage","1551"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Bao, Guobin"],["dc.contributor.author","Gomes de Castro, Maria Angela"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Phan, Nhu T. N."],["dc.date.accessioned","2021-06-01T09:41:35Z"],["dc.date.available","2021-06-01T09:41:35Z"],["dc.date.issued","2021"],["dc.description.abstract","The cellular functions of lipids in the neuronal plasma membranes have been increasingly acknowledged, particularly their association to neuronal processes and synaptic plasticity. However, the knowledge of their regulatory mechanisms in neuronal cells remains sparse. To address this, we investigated the lipid organization of the plasma membranes of hippocampal neurons in relation to neuronal activity using secondary ion mass spectrometry imaging. The neurons were treated with drugs, particularly tetrodotoxin (TTX) and bicuculline (BIC), to induce chronic activation and silencing. Distinct lipid organization was found in the plasma membrane of the cell body and the neurites. Moreover, significant alterations of the levels of the membrane lipids, especially ceramides, phosphatidylserines, phosphatidic acids, and triacylglycerols, were observed under the TTX and BIC treatments. We suggest that many types of membrane lipids are affected by, and may be involved in, the regulation of neuronal function."],["dc.identifier.doi","10.1021/acschemneuro.1c00031"],["dc.identifier.pmid","33896172"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84972"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/119"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation.eissn","1948-7193"],["dc.relation.issn","1948-7193"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 4.0"],["dc.title","Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal Activity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]