Schaap, Iwan A. T.

[["dc.bibliographiccitation.firstpage","237"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","242"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Stein, Simon"],["dc.contributor.author","Dekaliuk, Mariia O."],["dc.contributor.author","Battle, Christopher"],["dc.contributor.author","Li, Weixing"],["dc.contributor.author","Huss, Anja"],["dc.contributor.author","Platen, Mitja"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Demchenko, Alexander P."],["dc.contributor.author","Schmidt, Christoph F."],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey"],["dc.date.accessioned","2017-09-07T11:54:46Z"],["dc.date.available","2017-09-07T11:54:46Z"],["dc.date.issued","2016"],["dc.description.abstract","Success in super-resolution imaging relies on a proper choice of fluorescent probes. Here, we suggest novel easily produced and biocompatible nanoparticles-carbon nanodots-for super-resolution optical fluctuation bioimaging (SOFT). The particles revealed an intrinsic dual-color fluorescence, which corresponds to two subpopulations of particles of different electric charges. The neutral nanoparticles localize to cellular nuclei suggesting their potential use as an inexpensive, easily produced nucleus-specific label. The single particle study revealed that the carbon nanodots possess a unique hybrid combination of fluorescence properties exhibiting characteristics of both dye molecules and semiconductor nanocrystals. The results suggest that charge trapping and redistribution on the surface of the particles triggers their transitions between emissive and dark states. These findings open up new possibilities for the utilization of carbon nanodots in the various super-resolution microscopy methods based on stochastic optical switching."],["dc.identifier.doi","10.1021/acs.nanolett.5b03609"],["dc.identifier.gro","3141754"],["dc.identifier.isi","000368322700038"],["dc.identifier.pmid","26605640"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/702"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Super-Resolution Optical Fluctuation Bio-Imaging with Dual-Color Carbon Nanodots"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7868"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","7876"],["dc.bibliographiccitation.volume","291"],["dc.contributor.author","Milovanovic, Dragomir"],["dc.contributor.author","Platen, Mitja"],["dc.contributor.author","Junius, Meike"],["dc.contributor.author","Diederichsen, Ulf"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","Honigmann, Alf"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","van den Bogaart, Geert"],["dc.date.accessioned","2020-12-10T18:12:56Z"],["dc.date.available","2020-12-10T18:12:56Z"],["dc.date.issued","2016"],["dc.description.abstract","Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a minor component of total plasma membrane lipids, but it has a substantial role in the regulation of many cellular functions, including exo- and endocytosis. Recently, it was shown that PI(4,5)P2and syntaxin 1, a SNARE protein that catalyzes regulated exocytosis, form domains in the plasma membrane that constitute recognition sites for vesicle docking. Also, calcium was shown to promote syntaxin 1 clustering in the plasma membrane, but the molecular mechanism was unknown. Here, using a combination of superresolution stimulated emission depletion microscopy, FRET, and atomic force microscopy, we show that Ca(2+)acts as a charge bridge that specifically and reversibly connects multiple syntaxin 1/PI(4,5)P2complexes into larger mesoscale domains. This transient reorganization of the plasma membrane by physiological Ca(2+)concentrations is likely to be important for Ca(2+)-regulated secretion."],["dc.identifier.doi","10.1074/jbc.M116.716225"],["dc.identifier.eissn","1083-351X"],["dc.identifier.issn","0021-9258"],["dc.identifier.pmid","26884341"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13366"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74536"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","final"],["dc.relation.eissn","1083-351X"],["dc.relation.eissn","0021-9258"],["dc.rights","Goescholar"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.title","Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","954"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Nature Nanotechnology"],["dc.bibliographiccitation.lastpage","U194"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Dannhauser, Philip N."],["dc.contributor.author","Platen, Mitja"],["dc.contributor.author","Boening, Heike"],["dc.contributor.author","Schaap, I. A. T."],["dc.date.accessioned","2018-11-07T09:49:35Z"],["dc.date.available","2018-11-07T09:49:35Z"],["dc.date.issued","2015"],["dc.description.abstract","Biological molecules that self-assemble and interact with other molecules are attractive building blocks for engineering biological devices. DNA has been widely used for the creation of nanomaterials(1), but the use of proteins remains largely unexplored. Here, we show that clathrin can form homogeneous and extended two-dimensional lattices on a variety of substrates, including glass, metal, carbon and plastic. Clathrin is a threelegged protein complex with unique self-assembling properties and is relevant in the formation of membrane transport vesicles in eukaryotic cells(2,3). We used a fragment of the adaptor protein epsin to immobilize clathrin lattices on the substrates. The lattices span multiple square millimetres with a regular periodicity of 30 nm and can be functionalized via modified subunits of clathrin with either inorganic nanoparticles or active enzymes. The lattices can be stored for months after crosslinking and stabilization with uranyl acetate. They could be dehydrated and rehydrated without loss of function, offering potential applications in sensing and as biosynthetic reactors."],["dc.identifier.doi","10.1038/NNANO.2015.206"],["dc.identifier.isi","000364528300012"],["dc.identifier.pmid","26367107"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35538"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1748-3395"],["dc.relation.issn","1748-3387"],["dc.title","Durable protein lattices of clathrin that can be functionalized with nanoparticles and active biomolecules"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]