Schaap, Iwan A. T.

[["dc.bibliographiccitation.firstpage","2450"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2456"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","Carrasco, Carolina"],["dc.contributor.author","Pablo, Pedro J. de"],["dc.contributor.author","Schmidt, Christoph"],["dc.date.accessioned","2017-09-07T11:44:16Z"],["dc.date.available","2017-09-07T11:44:16Z"],["dc.date.issued","2011"],["dc.description.abstract","Motor proteins of the kinesin family move actively along microtubules to transport cargo within cells. How exactly a single motor proceeds on the 13 narrow lanes or protofilaments of a microtubule has not been visualized directly, and there persists controversy on the relative position of the two kinesin heads in different nucleotide states. We have succeeded in imaging Kinesin-1 dimers immobilized on microtubules with single-head resolution by atomic force microscopy. Moreover, we could catch glimpses of single Kinesin-1 dimers in their motion along microtubules with nanometer resolution. We find in our experiments that frequently both heads of one dimer are microtubule-bound at submicromolar ATP concentrations. Furthermore, we could unambiguously resolve that both heads bind to the same protofilament, instead of straddling two, and remain on this track during processive movement."],["dc.identifier.doi","10.1016/j.bpj.2011.04.015"],["dc.identifier.gro","3142727"],["dc.identifier.isi","000290830900016"],["dc.identifier.pmid","21575579"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/163"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.relation.issn","0006-3495"],["dc.title","Kinesin Walks the Line: Single Motors Observed by Atomic Force Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1521"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1531"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","Carrasco, Carolina"],["dc.contributor.author","Pablo, Pedro J. de"],["dc.contributor.author","MacKintosh, Frederick C."],["dc.contributor.author","Schmidt, Christoph"],["dc.date.accessioned","2017-09-07T11:52:37Z"],["dc.date.available","2017-09-07T11:52:37Z"],["dc.date.issued","2006"],["dc.description.abstract","We tested the mechanical properties of single microtubules by lateral indentation with the tip of an atomic force microscope. Indentations up to similar to 3.6 nm, i.e., 15% of the microtubule diameter, resulted in an approximately linear elastic response, and indentations were reversible without hysteresis. At an indentation force of around 0.3 nN we observed an instability corresponding to an similar to 1-nm indentation step in the taxol-stabilized microtubules, which could be due to partial or complete rupture of a relatively small number of lateral or axial tubulin-tubulin bonds. These indentations were reversible with hysteresis when the tip was retracted and no trace of damage was observed in subsequent high-resolution images. Higher forces caused substantial damage to the microtubules, which either led to depolymerization or, occasionally, to slowly reannealing holes in the microtubule wall. We modeled the experimental results using finite-element methods and find that the simple assumption of a homogeneous isotropic material, albeit structured with the characteristic proto. lament corrugations, is sufficient to explain the linear elastic response of microtubules."],["dc.identifier.doi","10.1529/biophysj.105.077826"],["dc.identifier.gro","3143644"],["dc.identifier.isi","000239242000037"],["dc.identifier.pmid","16731557"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1181"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Elastic response, buckling, and instability of microtubules under radial indentation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","098101"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Pablo, Pedro J. de"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","MacKintosh, F. C."],["dc.contributor.author","Schmidt, Christoph"],["dc.date.accessioned","2017-09-07T11:44:15Z"],["dc.date.available","2017-09-07T11:44:15Z"],["dc.date.issued","2003"],["dc.description.abstract","We probe the local mechanical properties of microtubules at the nanometer scale by radial indentation with a scanning force microscope tip. We find a linear elastic regime that can be described by both thin-shell theory and finite element methods, in which microtubules are modeled as hollow tubes. We also find a nonlinear regime and catastrophic collapse of the microtubules under large loads. The main physics of protein shells at the nanometer scale shows simultaneously aspects of continuum elasticity in their linear response, as well as molecular graininess in their nonlinear behavior."],["dc.identifier.doi","10.1103/PhysRevLett.91.098101"],["dc.identifier.gro","3144066"],["dc.identifier.isi","000185235000054"],["dc.identifier.pmid","14525215"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1649"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","American Physical Soc"],["dc.relation.issn","0031-9007"],["dc.title","Deformation and collapse of microtubules on the nanometer scale"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","462"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.lastpage","467"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Schaap, Iwan A. T."],["dc.contributor.author","Pablo, Pedro J. de"],["dc.contributor.author","Schmidt, Christoph"],["dc.date.accessioned","2017-09-07T11:43:19Z"],["dc.date.available","2017-09-07T11:43:19Z"],["dc.date.issued","2004"],["dc.description.abstract","We have imaged microtubules, essential structural elements of the cytoskeleton in eukaryotic cells, in physiological conditions by scanning force microscopy. We have achieved molecular resolution without the use of cross-linking and chemical fixation methods. With tip forces below 0.3 nN, protofilaments with similar to6 nm separation could be clearly distinguished. Lattice defects in the microtubule wall were directly visible, including point defects and protofilament separations. Higher tip forces destroyed the top half of the microtubules, revealing the inner surface of the substrate-attached protofilaments. Monomers could be resolved on these inner surfaces."],["dc.identifier.doi","10.1007/s00249-003-0386-8"],["dc.identifier.gro","3143961"],["dc.identifier.isi","000223474100009"],["dc.identifier.pmid","14762705"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1532"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Springer"],["dc.relation.issn","0175-7571"],["dc.title","Resolving the molecular structure of microtubules under physiological conditions with scanning force microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]