Niebert, Marcus

[["dc.bibliographiccitation.firstpage","1821"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Biomaterials"],["dc.bibliographiccitation.lastpage","1829"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Ladewig, Katharina"],["dc.contributor.author","Niebert, Marcus"],["dc.contributor.author","Xu, Zhi Ping"],["dc.contributor.author","Gray, Peter P."],["dc.contributor.author","Lu, G. Q."],["dc.date.accessioned","2018-11-07T08:45:12Z"],["dc.date.available","2018-11-07T08:45:12Z"],["dc.date.issued","2010"],["dc.description.abstract","Although siRNAs have surpassed expectations in experiments to alter gene expression in vitro, the lack of an efficient in vivo delivery system still remains a challenge in siRNA therapeutics development and has been recognized as a major hurdle for clinical applications. in this paper we describe an inorganic nanoparticle-based delivery system that is readily adaptable for in vivo systems. Layered double hydroxide (LDH) nanoparticles, a family of inorganic crystals, tightly bind, protect, and release siRNA molecules and deliver them efficiently to mammalian cells in vitro. The uptake of siRNA-loaded LDH nanoparticles occurs via endocytosis, whereby the nanoparticles dissolve due to the low pH in the endosome, thereby aiding endosomal escape into the cytoplasm. The influence of LDH nanoparticles on cell viability and proliferation is negligible at concentrations <= 0.050 mg mL(-1), and a pronounced down-regulation of protein expression upon LDH mediated siRNA transfection of HEK293T cells is observed. (C) 2009 Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.biomaterials.2009.10.058"],["dc.identifier.isi","000274354400040"],["dc.identifier.pmid","19922997"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20382"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.relation.issn","0142-9612"],["dc.title","Efficient siRNA delivery to mammalian cells using layered double hydroxide nanoparticles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","280"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Applied Clay Science"],["dc.bibliographiccitation.lastpage","289"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Ladewig, Katharina"],["dc.contributor.author","Niebert, Marcus"],["dc.contributor.author","Xu, Zhi Ping"],["dc.contributor.author","Gray, Peter P."],["dc.contributor.author","Lu, G. Q."],["dc.date.accessioned","2018-11-07T08:45:09Z"],["dc.date.available","2018-11-07T08:45:09Z"],["dc.date.issued","2010"],["dc.description.abstract","Layered double hydroxides (LDHs) have been known for many decades as catalyst and ceramic precursors, traps for anionic pollutants, catalysts, and additives for polymers, but they recently attracted attention as potential nano-sized carriers for therapeutic/bio-active molecules and genes. Among the many different nanoparticles that have been shown to facilitate gene and/or drug delivery, LDH nanoparticles are particularly well suited for this purpose due to their many desirable properties. In this research Mg(2)Al(OH)(6)NO(3) LDH nanoparticles of varying lateral sizes were synthesized by altering the synthesis conditions. The synthesis conditions particularly influencing the particle size distribution of the LDH suspensions are (a) the temperature during the co-precipitation step and (b) the duration and the temperature of the hydrothermal treatment The association of these nanoparticles with plasmid DNA was studied and it was established that-in contrast to previously published reports-for the plasmid sizes used no significant intercalation occurs. The plasmids wrap around individual particles instead and aggregation of particles is observed. However, due to the observed strong interaction between LDH nanoparticles and DNA, the particles were nonetheless evaluated as transfection agents for mammalian cells. Considerable transfection efficiencies when transfecting adherent cell lines (i.e., HEK293T, NIH 3T3, COS-7, and CHO-K1) were observed, while the transfection of suspension CHO-S cells remained unsuccessful. This is attributed to the formation of aggregates upon DNA-LDH complex formation which settle on top of adherent cells but due to the constant agitation of suspension cultures not on the surface of e.g., CHO-S cells. (C) 2009 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.clay.2009.11.032"],["dc.identifier.isi","000276010700041"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20367"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0169-1317"],["dc.title","Controlled preparation of layered double hydroxide nanoparticles and their application as gene delivery vehicles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]