Masanta, Wycliffe Omurwa

[["dc.bibliographiccitation.firstpage","1019"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","European Journal of Clinical Microbiology & Infectious Diseases"],["dc.bibliographiccitation.lastpage","1027"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Johann, C."],["dc.contributor.author","Strubel, A."],["dc.contributor.author","Busse, C."],["dc.contributor.author","Tareen, Abdul Malik"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Schmidt-Ott, Ruprecht"],["dc.contributor.author","Gross, U."],["dc.date.accessioned","2018-11-07T09:39:47Z"],["dc.date.available","2018-11-07T09:39:47Z"],["dc.date.issued","2014"],["dc.description.abstract","Post-infectious sequelea such as Guillain Barr, syndrome (GBS), reactive arthritis (RA), and inflammatory bowel disease (IBD) may arise as a consequence of acute Campylobacter-enteritis (AE). However, reliable seroprevalence data of Campylobacter-associated sequelae has not been established. The objectives of this study were, first, to identify the most specific and sensitive test antigen in an optimized ELISA assay for diagnosing a previous Campylobacter-infection and, second, to compare the prevalence of anti-Campylobacter antibodies in cohorts of healthy blood donors (BD), AE, GBS, RA, and IBD patients with antibodies against known GBS, RA and IBD triggering pathogens. Optimized ELISAs of single and combined Campylobacter-proteins OMP18 and P39 as antigens were prepared and sera from AE, GBS, RA and IBD patients and BD were tested for Campylobcter-specific IgA and IgG antibodies. The results were compared with MIKROGEN (TM)-recomLine Campylobacter IgA/IgG and whole cell lysate-immunoblot. Antibodies specific for Helicobacter pylori, Mycoplasma pneumoniae, Yersinia enterocolitica, and Borrelia afzelii were tested with commercial immunoblots. ROC plot analysis revealed AUC maxima in the combination of OMP18 and P39 for IgA and in the P39-antigen for IgG. As a result, 34-49 % GBS cases, 44-62 % RA cases and 23-40 % IBD cases were associated with Campylobacter-infection. These data show that Campylobcater-seropositivity in these patient groups is significantly higher than other triggering pathogens suggesting that it plays an important role in development of GBS and RA, and supports the hypothesis that recurrent acute campylobacteriosis triggers IBD."],["dc.identifier.doi","10.1007/s10096-013-2040-4"],["dc.identifier.isi","000335743500017"],["dc.identifier.pmid","24413899"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9697"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33365"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1435-4373"],["dc.relation.issn","0934-9723"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Seroprevalence of campylobacteriosis and relevant post-infectious sequelae"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","267"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","European Journal of Microbiology and Immunology"],["dc.bibliographiccitation.lastpage","273"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Masanta, Wycliffe O."],["dc.contributor.author","Linsel, Gunter"],["dc.contributor.author","Heutelbeck, Astrid"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2020-12-10T18:42:43Z"],["dc.date.available","2020-12-10T18:42:43Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1556/1886.2017.00024"],["dc.identifier.eissn","2062-8633"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78057"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Seroprevalence of Chlamydophila psittaci among employees of two German duck farms"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","13431"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T09:53:08Z"],["dc.date.available","2018-11-07T09:53:08Z"],["dc.date.issued","2015"],["dc.description.abstract","MALDI-TOF-MS of microorganisms, which identifies microbes based on masses of high abundant low molecular weight proteins, is rapidly advancing to become another standard method in clinical routine laboratory diagnostics. Allelic isoforms of these proteins result in varying masses of detectable biomarker ions. These variations give rise to a novel typing method for microorganisms named mass spectrometry-based phyloproteomics (MSPP). The base of MSPP is an amino acid sequence list of allelic isoforms caused by non-synonymous mutations in biomarker genes, which were detectable as mass shifts in an overlay of calibrated MALDI-TOF spectra. Thus, for each isolate a combination of amino acid sequences can be deduced from the scheme of recordable biomarker masses. Performing comparably to laborious multilocus and whole genome sequence typing (wgMLST)-approaches it is feasible to build phyloproteomic dendrograms using hierarchical cluster analysis. MSPP bears a high potential especially for identification of chromosomal localised virulence or antimicrobial resistance factors associated with evolutionary relatedness. In this study the principle of MSPP-typing was demonstrated on a Campylobacter jejuni ssp. jejuni isolate collection and MSPP was compared to MLST."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft; Georg August Universitat Gottingen"],["dc.identifier.doi","10.1038/srep13431"],["dc.identifier.isi","000360037600002"],["dc.identifier.pmid","26303099"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12455"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36271"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Mass Spectrometry-based PhyloProteomics (MSPP): A novel microbial typing Method"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","European Journal of Microbiology and Immunology"],["dc.bibliographiccitation.lastpage","16"],["dc.contributor.author","Mund, Norah Lynn-Anne"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Goldschmidt, Anne-Marie"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2019-07-09T11:42:30Z"],["dc.date.available","2019-07-09T11:42:30Z"],["dc.date.issued","2016"],["dc.description.abstract","Campylobacter jejuni’s flagellar locomotion is controlled by eleven chemoreceptors. Assessment of the distribution of the relevant chemoreceptor genes in the C. jejuni genomes deposited in the National Center for Biotechnology Information (NCBI) database led to the identification of two previously unknown tlp genes and a tlp5 pseudogene. These two chemoreceptor genes share the same locus in the C. jejuni genome with tlp4 and tlp11, but the gene region encoding the periplasmic ligand binding domain differs significantly from other chemoreceptor genes. Hence, they were named tlp12 and tlp13. Consequently, it was of interest to study their distribution in C. jejuni subpopulations of different clonality, and their cooccurrence with the eleven previously reported chemoreceptor genes. Therefore, the presence of all tlp genes was detected by polymerase chain reaction (PCR) in 292 multilocus sequence typing (MLST)-typed C. jejuni isolates from different hosts. The findings show interesting trends: Tlp4, tlp11, tlp12, and tlp13 appeared to be mutually exclusive and cooccur in a minor subset of isolates. Tlp4 was found to be present in only 33.56% of all tested isolates and was significantly less often detected in turkey isolates. Tlp11 was tested positive in only 17.8% of the isolates, while tlp12 was detected in 29.5% of all isolates, and tlp13 was found to be present in 38.7%."],["dc.identifier.doi","10.1556/1886.2015.00041"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13542"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58681"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2062-8633"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Association of Campylobacter jejuni ssp. Jejuni chemotaxis receptor genes with multilocus sequence types and source of isolation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","80"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","81"],["dc.bibliographiccitation.volume","303"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Tareen, Abdul Malik"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Gross, U."],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2018-11-07T09:20:05Z"],["dc.date.available","2018-11-07T09:20:05Z"],["dc.date.issued","2013"],["dc.identifier.isi","000331497600276"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28794"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","65th Annual Meeting of the German-Society-for-Hygiene-and-Microbiology (DGHM) e V / Annual Meeting of the German-Society-for-Infectious-Diseases (DGI) e V"],["dc.relation.eventlocation","Univ Rostock, Rostock, GERMANY"],["dc.relation.issn","1618-0607"],["dc.relation.issn","1438-4221"],["dc.title","Phyloproteomics versus phylogenetics: a comparative approach for the Discrimination of Campylobacter jejuni Subpopulations"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","4244"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Emele, Matthias Frederik"],["dc.contributor.author","Možina, Sonja Smole"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.date.accessioned","2019-07-09T11:50:14Z"],["dc.date.available","2019-07-09T11:50:14Z"],["dc.date.issued","2019"],["dc.description.abstract","Besides Campylobacter jejuni, Campylobacter coli is the most common bacterial cause of gastroenteritis worldwide. C. coli is subdivided into three clades, which are associated with sample source. Clade 1 isolates are associated with acute diarrhea in humans whereas clade 2 and 3 isolates are more commonly obtained from environmental waters. The phylogenetic classification of an isolate is commonly done using laborious multilocus sequence typing (MLST). The aim of this study was to establish a proteotyping scheme using MALDI-TOF MS to offer an alternative to sequence-based methods. A total of 97 clade-representative C. coli isolates were analyzed by MALDI-TOF-based intact cell mass spectrometry (ICMS) and evaluated to establish a C. coli proteotyping scheme. MLST was used as reference method. Different isoforms of the detectable biomarkers, resulting in biomarker mass shifts, were associated with their amino acid sequences and included into the C. coli proteotyping scheme. In total, we identified 16 biomarkers to differentiate C. coli into the three clades and three additional sub-clades of clade 1. In this study, proteotyping has been successfully adapted to C. coli. The established C. coli clades and sub-clades can be discriminated using this method. Especially the clinically relevant clade 1 isolates can be differentiated clearly."],["dc.identifier.doi","10.1038/s41598-019-40842-w"],["dc.identifier.pmid","30862911"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15886"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59727"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Proteotyping as alternate typing method to differentiate Campylobacter coli clades"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1800083"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PROTEOMICS – Clinical Applications"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Masanta, Wycliffe O."],["dc.contributor.author","Zautner, Andreas E."],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Leha, Andreas"],["dc.contributor.author","Dakna, Mohammed"],["dc.contributor.author","Lenz, Christof"],["dc.date.accessioned","2019-07-09T11:51:53Z"],["dc.date.available","2019-07-09T11:51:53Z"],["dc.date.issued","2018"],["dc.description.abstract","PURPOSE: Bile acids are crucial components of the intestinal antimicrobial defense and represent a significant stress factor for enteric pathogens. Adaptation processes of Campylobacter jejuni to this hostile environment are analyzed in this study by a proteomic approach. EXPERIMENTAL DESIGN: Proteome profiling by label-free mass spectrometry (SWATH-MS) has been used to characterize the adaptation of C. jejuni to sublethal concentrations of seven bile acids. RESULTS: The bile acids with the lowest inhibitory concentration (IC50 ), deoxycholic and chenodeoxycholic acid, induce the most significant proteome changes. Overall a downregulation of all basic biosynthetic pathways and a general decrease in the transcription machinery are found. Concurrently, an induction of factors involved in detoxification of reactive oxygen species, protein folding, and bile acid exporting efflux pumps is detected. Exposure to deoxycholic and chenodeoxycholic acid results in an increased expression of components of the more energy-efficient aerobic respiration pathway, while the anaerobic branches of the electron transport chain are down-expressed. CONCLUSIONS AND CLINICAL RELEVANCE: The results show that C. jejuni has a differentiated system of adaptation to bile acid stresses. The findings enhance the understanding of the pathogenesis of campylobacteriosis, especially for survival of C. jejuni in the human intestine, and may provide clues to future medical treatment."],["dc.identifier.doi","10.1002/prca.201800083"],["dc.identifier.pmid","30246935"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16217"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60032"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Proteome Profiling by Label‐Free Mass Spectrometry Reveals Differentiated Response of Campylobacter jejuni 81–176 to Sublethal Concentrations of Bile Acids"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","526860"],["dc.bibliographiccitation.journal","Clinical and Developmental Immunology"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Heimesaat, Markus M."],["dc.contributor.author","Bereswill, Stefan"],["dc.contributor.author","Tareen, Abdul Malik"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.date.accessioned","2018-11-07T09:29:31Z"],["dc.date.available","2018-11-07T09:29:31Z"],["dc.date.issued","2013"],["dc.description.abstract","Campylobacter jejuni is the leading cause of bacterial food-borne gastroenteritis in the world, and thus one of the most important public health concerns. The initial stage in its pathogenesis after ingestion is to overcome colonization resistance that is maintained by the human intestinal microbiota. But how it overcomes colonization resistance is unknown. Recently developed humanized gnotobiotic mouse models have provided deeper insights into this initial stage and host's immune response. These studies have found that a fat-rich diet modifies the composition of the conventional intestinal microbiota by increasing the Firmicutes and Proteobacteria loads while reducing the Actinobacteria and Bacteroidetes loads creating an imbalance that exposes the intestinal epithelial cells to adherence. Upon adherence, deoxycholic acid stimulates C. jejuni to synthesize Campylobacter invasion antigens, which invade the epithelial cells. In response, NF-kappa B triggers the maturation of dendritic cells. Chemokines produced by the activated dendritic cells initiate the clearance of C. jejuni cells by inducing the actions of neutrophils, B-lymphocytes, and various subsets of T-cells. This immune response causes inflammation. This review focuses on the progress that has been made on understanding the relationship between intestinal microbiota shift, establishment of C. jejuni infection, and consequent immune response."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2013"],["dc.identifier.doi","10.1155/2013/526860"],["dc.identifier.isi","000327262600001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9506"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31052"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Hindawi Publishing Corporation"],["dc.relation.issn","1740-2530"],["dc.relation.issn","1740-2522"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Modification of Intestinal Microbiota and Its Consequences for Innate Immune Response in the Pathogenesis of Campylobacteriosis"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","116"],["dc.bibliographiccitation.journal","Journal of Visualized Experiments"],["dc.contributor.author","Zautner, Andreas E."],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Masanta, Wycliffe O."],["dc.contributor.author","Weig, Michael"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.date.accessioned","2020-12-10T18:47:29Z"],["dc.date.available","2020-12-10T18:47:29Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.3791/54165"],["dc.identifier.eissn","1940-087X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78780"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","118"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","European Journal of Microbiology and Immunology"],["dc.bibliographiccitation.lastpage","123"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Linsel, Gunter"],["dc.contributor.author","Heutelbeck, Astrid"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.date.accessioned","2019-07-09T11:43:02Z"],["dc.date.available","2019-07-09T11:43:02Z"],["dc.date.issued","2016"],["dc.description.abstract","Several studies have shown that about 60–100% of farmed ducks are colonized by Campylobacter species. Because of this, a higher risk of campylobacteriosis among duck farm workers can be assumed. To estimate the risk of Campylobacter infections in duck farm workers, we investigated the prevalence of Campylobacter spp. in ducks of two duck farms and the seroprevalence of anti-Campylobacter antibodies (IgA and IgG) in two cohorts of workers. The first cohort consisted of high-exposed stable workers and slaughterers, which was compared to a second cohort of non-/low-exposed persons. Duck caecal swabs and serum samples were collected in 2004, 2007, and 2010. The colonization rate in the examined ducks was found to be 80–90%. The seroprevalence of anti-Campylobacter IgA and IgG antibodies among the non-exposed cohort was found to be 0.00% in all 3 years. In contrast, the exposed cohort demonstrated an IgA seroprevalence of 4.17% in 2004, 5.71% in 2007, and 0.00% in 2010 and an IgG seroprevalence of 8.33% in 2004, 0.00% in 2007, and 4.29% in 2010. In conclusion, in 2004, we observed a significantly higher anti-Campylobacter antibody seroprevalence in the exposed cohort followed by a steady reduction in 2007 and 2010 under occupational health and safety measures."],["dc.identifier.doi","10.1556/1886.2016.00007"],["dc.identifier.pmid","27429794"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14085"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58807"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2062-8633"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Seroprevalence of Campylobacter-Specific Antibodies in two German Duck Farms — A Prospective Follow-Up Study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]