Popko, Jennifer

[["dc.bibliographiccitation.artnumber","e0164673"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Popko, Jennifer"],["dc.contributor.author","Herrfurth, Cornelia"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Iven, Tim"],["dc.contributor.author","Haslam, Richard P."],["dc.contributor.author","Hamilton, Mary"],["dc.contributor.author","Sayanova, Olga"],["dc.contributor.author","Napier, Jonathan"],["dc.contributor.author","Khozin-Goldberg, Inna"],["dc.contributor.author","Feussner, Ivo"],["dc.date.accessioned","2018-11-07T10:07:03Z"],["dc.date.available","2018-11-07T10:07:03Z"],["dc.date.issued","2016"],["dc.description.abstract","Oleaginous microalgae are considered as a promising resource for the production of biofuels. Especially diatoms arouse interest as biofuel producers since they are most productive in carbon fixation and very flexible to environmental changes in the nature. Naturally, triacylglycerol (TAG) accumulation in algae only occurs under stress conditions like nitrogenlimitation. We focused on Phaeodactylum strain Pt4 (UTEX 646), because of its ability to grow in medium with low salinity and therefore being suited when saline water is less available or for wastewater cultivation strategies. Our data show an increase in neutral lipids during nitrogen-depletion and predominantly 16: 0 and 16: 1(n-7) accumulated in the TAG fraction. The molecular species composition of TAG suggests a remodeling primarily from the betaine lipid diacylglyceroltrimethylhomoserine (DGTS), but a contribution of the chloroplast galactolipid monogalactosyldiacylglycerol (MGDG) cannot be excluded. Interestingly, the acyl-CoA pool is rich in 20: 5(n-3) and 22: 6(n-3) in all analyzed conditions, but these fatty acids are almost excluded from TAG. Other metabolites most obviously depleted under nitrogen-starvation were amino acids, lyso-phospholipids and tricarboxylic acid (TCA) cycle intermediates, whereas sulfur-containing metabolites as dimethylsulfoniopropionate, dimethylsulfoniobutyrate and methylsulfate as well as short acyl chain carnitines, propanoyl-carnitine and butanoyl-carnitine increased upon nitrogen-starvation. Moreover, the Calvin cycle may be de-regulated since sedoheptulose accumulated after nitrogen-depletion. Together the data provide now the basis for new strategies to improve lipid production and storage in Phaeodactylum strain Pt4."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0164673"],["dc.identifier.isi","000385505800110"],["dc.identifier.pmid","27736949"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13799"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39211"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Metabolome Analysis Reveals Betaine Lipids as Major Source for Triglyceride Formation, and the Accumulation of Sedoheptulose during Nitrogen-Starvation of Phaeodactylum tricornutum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2773"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Applied Phycology"],["dc.bibliographiccitation.lastpage","2791"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Sitnik, Siarhei"],["dc.contributor.author","Shtaida, Nastassia"],["dc.contributor.author","Guihéneuf, Freddy"],["dc.contributor.author","Leu, Stefan"],["dc.contributor.author","Popko, Jennifer"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Boussiba, Sammy"],["dc.contributor.author","Khozin-Goldberg, Inna"],["dc.date.accessioned","2020-12-10T14:11:38Z"],["dc.date.available","2020-12-10T14:11:38Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1007/s10811-017-1364-3"],["dc.identifier.eissn","1573-5176"],["dc.identifier.issn","0921-8971"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71142"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","DGAT1 from the arachidonic-acid-producing microalga Lobosphaera incisa shows late gene expression under nitrogen starvation and substrate promiscuity in a heterologous system"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","98"],["dc.bibliographiccitation.journal","BMC Plant Biology"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Siegler, Heike"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Popko, Jennifer"],["dc.contributor.author","Tourasse, Nicolas J."],["dc.contributor.author","Vallon, Olivier"],["dc.contributor.author","Khozin-Goldberg, Inna"],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","Feussner, Ivo"],["dc.date.accessioned","2018-11-07T10:22:49Z"],["dc.date.available","2018-11-07T10:22:49Z"],["dc.date.issued","2017"],["dc.description.abstract","Background: Lobosphaera incisa (L. incisa) is an oleaginous microalga that stores triacylglycerol (TAG) rich in arachidonic acid in lipid bodies (LBs). This organelle is gaining attention in algal research, since evidence is accumulating that proteins attached to its surface fulfill important functions in TAG storage and metabolism. Results: Here, the composition of the LB proteome in L incisa was investigated by comparing different cell fractions in a semiquantitative proteomics approach. After applying stringent filters to the proteomics data in order to remove contaminating proteins from the list of possible LB proteins (LBPs), heterologous expression of candidate proteins in tobacco pollen tubes, allowed us to confirm 3 true LBPs: A member of the algal Major Lipid Droplet Protein family, a small protein of unknown function and a putative lipase. In addition, a TAG lipase that belongs to the SUGAR DEPENDENT 1 family of TAG lipases known from oilseed plants was identified. Its activity was verified by functional complementation of an Arabidopsis thaliana mutant lacking the major seed TAG lipases. Conclusions: Here we describe 3 LBPs as well as a TAG lipase from the oleaginous microalga L. incisa and discuss their possible involvement in LB metabolism. This study highlights the importance of filtering LB proteome datasets and verifying the subcellular localization one by one, so that contaminating proteins can be recognized as such. Our dataset can serve as a valuable resource in the identification of additional LBPs, shedding more light on the intriguing roles of LBs in microalgae."],["dc.identifier.doi","10.1186/s12870-017-1042-2"],["dc.identifier.isi","000403879900002"],["dc.identifier.pmid","28587627"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14506"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42344"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2229"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]