Popko, Jennifer

[["dc.bibliographiccitation.firstpage","392"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","The Plant Journal"],["dc.bibliographiccitation.lastpage","404"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Pommerrenig, Benjamin"],["dc.contributor.author","Popko, Jennifer"],["dc.contributor.author","Heilmann, Mareike"],["dc.contributor.author","Schulmeister, Sylwia"],["dc.contributor.author","Dietel, Katharina"],["dc.contributor.author","Schmitt, Bianca"],["dc.contributor.author","Stadler, Ruth"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Sauer, Norbert"],["dc.date.accessioned","2018-11-07T09:28:41Z"],["dc.date.available","2018-11-07T09:28:41Z"],["dc.date.issued","2013"],["dc.description.abstract","The Arabidopsis SUC5 protein represents a classical sucrose/H+ symporter. Functional analyses previously revealed that SUC5 also transports biotin, an essential co-factor for fatty acid synthesis. However, evidence for a dual role in transport of the structurally unrelated compounds sucrose and biotin in plants was lacking. Here we show that SUC5 localizes to the plasma membrane, and that the SUC5 gene is expressed in developing embryos, confirming the role of the SUC5 protein as substrate carrier across apoplastic barriers in seeds. We show that transport of biotin but not of sucrose across these barriers is impaired in suc5 mutant embryos. In addition, we show that SUC5 is essential for the delivery of biotin into the embryo of biotin biosynthesis-defective mutants (bio1 and bio2). We compared embryo and seedling development as well as triacylglycerol accumulation and fatty acid composition in seeds of single mutants (suc5, bio1 or bio2), double mutants (suc5bio1 and suc5bio2) and wild-type plants. Although suc5 mutants were like the wild-type, bio1 and bio2 mutants showed developmental defects and reduced triacylglycerol contents. In suc5bio1 and suc5bio2 double mutants, developmental defects were severely increased and the triacylglycerol content was reduced to a greater extent in comparison to the single mutants. Supplementation with externally applied biotin helped to reduce symptoms in both single and double mutants, but the efficacy of supplementation was significantly lower in double than in single mutants, showing that transport of biotin into the embryo is lower in the absence of SUC5."],["dc.description.sponsorship","German Bundesministerium fur Bildung und Forschung (BMBF) [Fkz 0315429]"],["dc.identifier.doi","10.1111/tpj.12037"],["dc.identifier.isi","000314184600004"],["dc.identifier.pmid","23031218"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10988"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30840"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0960-7412"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","SUCROSE TRANSPORTER 5 supplies Arabidopsis embryos with biotin and affects triacylglycerol accumulation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0164673"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Popko, Jennifer"],["dc.contributor.author","Herrfurth, Cornelia"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Iven, Tim"],["dc.contributor.author","Haslam, Richard P."],["dc.contributor.author","Hamilton, Mary"],["dc.contributor.author","Sayanova, Olga"],["dc.contributor.author","Napier, Jonathan"],["dc.contributor.author","Khozin-Goldberg, Inna"],["dc.contributor.author","Feussner, Ivo"],["dc.date.accessioned","2018-11-07T10:07:03Z"],["dc.date.available","2018-11-07T10:07:03Z"],["dc.date.issued","2016"],["dc.description.abstract","Oleaginous microalgae are considered as a promising resource for the production of biofuels. Especially diatoms arouse interest as biofuel producers since they are most productive in carbon fixation and very flexible to environmental changes in the nature. Naturally, triacylglycerol (TAG) accumulation in algae only occurs under stress conditions like nitrogenlimitation. We focused on Phaeodactylum strain Pt4 (UTEX 646), because of its ability to grow in medium with low salinity and therefore being suited when saline water is less available or for wastewater cultivation strategies. Our data show an increase in neutral lipids during nitrogen-depletion and predominantly 16: 0 and 16: 1(n-7) accumulated in the TAG fraction. The molecular species composition of TAG suggests a remodeling primarily from the betaine lipid diacylglyceroltrimethylhomoserine (DGTS), but a contribution of the chloroplast galactolipid monogalactosyldiacylglycerol (MGDG) cannot be excluded. Interestingly, the acyl-CoA pool is rich in 20: 5(n-3) and 22: 6(n-3) in all analyzed conditions, but these fatty acids are almost excluded from TAG. Other metabolites most obviously depleted under nitrogen-starvation were amino acids, lyso-phospholipids and tricarboxylic acid (TCA) cycle intermediates, whereas sulfur-containing metabolites as dimethylsulfoniopropionate, dimethylsulfoniobutyrate and methylsulfate as well as short acyl chain carnitines, propanoyl-carnitine and butanoyl-carnitine increased upon nitrogen-starvation. Moreover, the Calvin cycle may be de-regulated since sedoheptulose accumulated after nitrogen-depletion. Together the data provide now the basis for new strategies to improve lipid production and storage in Phaeodactylum strain Pt4."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0164673"],["dc.identifier.isi","000385505800110"],["dc.identifier.pmid","27736949"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13799"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39211"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Metabolome Analysis Reveals Betaine Lipids as Major Source for Triglyceride Formation, and the Accumulation of Sedoheptulose during Nitrogen-Starvation of Phaeodactylum tricornutum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Biotechnology for Biofuels"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Zulu, Nodumo Nokulunga"],["dc.contributor.author","Popko, Jennifer"],["dc.contributor.author","Zienkiewicz, Krzysztof"],["dc.contributor.author","Tarazona, Pablo"],["dc.contributor.author","Herrfurth, Cornelia"],["dc.contributor.author","Feussner, Ivo"],["dc.date.accessioned","2019-07-09T11:43:44Z"],["dc.date.available","2019-07-09T11:43:44Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1186/s13068-017-0874-1"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15143"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58962"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/14665 but duplicate"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Heterologous co-expression of a yeast diacylglycerol acyltransferase (ScDGA1) and a plant oleosin (AtOLEO3) as an efficient tool for enhancing triacylglycerol accumulation in the marine diatom Phaeodactylum tricornutum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","61"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Plant molecular biology"],["dc.bibliographiccitation.lastpage","75"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Behnke, Katja"],["dc.contributor.author","Kaiser, Andreas"],["dc.contributor.author","Zimmer, Ina"],["dc.contributor.author","Brüggemann, Nicolas"],["dc.contributor.author","Janz, Dennis"],["dc.contributor.author","Polle, Andrea"],["dc.contributor.author","Hampp, Rüdiger"],["dc.contributor.author","Hänsch, Robert"],["dc.contributor.author","Popko, Jennifer"],["dc.contributor.author","Schmitt-Kopplin, Philippe"],["dc.contributor.author","Ehlting, Barbara"],["dc.contributor.author","Rennenberg, Heinz"],["dc.contributor.author","Barta, Csengele"],["dc.contributor.author","Loreto, Francesco"],["dc.contributor.author","Schnitzler, Jörg-Peter"],["dc.date.accessioned","2018-06-12T08:31:25Z"],["dc.date.available","2018-06-12T08:31:25Z"],["dc.date.issued","2010"],["dc.description.abstract","In plants, isoprene plays a dual role: (a) as thermo-protective agent proposed to prevent degradation of enzymes/membrane structures involved in photosynthesis, and (b) as reactive molecule reducing abiotic oxidative stress. The present work addresses the question whether suppression of isoprene emission interferes with genome wide transcription rates and metabolite fluxes in grey poplar (Populus x canescens) throughout the growing season. Gene expression and metabolite profiles of isoprene emitting wild type plants and RNAi-mediated non-isoprene emitting poplars were compared by using poplar Affymetrix microarrays and non-targeted FT-ICR-MS (Fourier transform ion cyclotron resonance mass spectrometry). We observed a transcriptional down-regulation of genes encoding enzymes of phenylpropanoid regulatory and biosynthetic pathways, as well as distinct metabolic down-regulation of condensed tannins and anthocyanins, in non-isoprene emitting genotypes during July, when high temperature and light intensities possibly caused transient drought stress, as indicated by stomatal closure. Under these conditions leaves of non-isoprene emitting plants accumulated hydrogen peroxide (H(2)O(2)), a signaling molecule in stress response and negative regulator of anthocyanin biosynthesis. The absence of isoprene emission under high temperature and light stress resulted transiently in a new chemo(pheno)type with suppressed production of phenolic compounds. This may compromise inducible defenses and may render non-isoprene emitting poplars more susceptible to environmental stress."],["dc.identifier.doi","10.1007/s11103-010-9654-z"],["dc.identifier.pmid","20526857"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6811"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15024"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1573-5028"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","RNAi-mediated suppression of isoprene emission in poplar transiently impacts phenolic metabolism under high temperature and high light intensities: a transcriptomic and metabolomic analysis"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","98"],["dc.bibliographiccitation.journal","BMC Plant Biology"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Siegler, Heike"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Popko, Jennifer"],["dc.contributor.author","Tourasse, Nicolas J."],["dc.contributor.author","Vallon, Olivier"],["dc.contributor.author","Khozin-Goldberg, Inna"],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","Feussner, Ivo"],["dc.date.accessioned","2018-11-07T10:22:49Z"],["dc.date.available","2018-11-07T10:22:49Z"],["dc.date.issued","2017"],["dc.description.abstract","Background: Lobosphaera incisa (L. incisa) is an oleaginous microalga that stores triacylglycerol (TAG) rich in arachidonic acid in lipid bodies (LBs). This organelle is gaining attention in algal research, since evidence is accumulating that proteins attached to its surface fulfill important functions in TAG storage and metabolism. Results: Here, the composition of the LB proteome in L incisa was investigated by comparing different cell fractions in a semiquantitative proteomics approach. After applying stringent filters to the proteomics data in order to remove contaminating proteins from the list of possible LB proteins (LBPs), heterologous expression of candidate proteins in tobacco pollen tubes, allowed us to confirm 3 true LBPs: A member of the algal Major Lipid Droplet Protein family, a small protein of unknown function and a putative lipase. In addition, a TAG lipase that belongs to the SUGAR DEPENDENT 1 family of TAG lipases known from oilseed plants was identified. Its activity was verified by functional complementation of an Arabidopsis thaliana mutant lacking the major seed TAG lipases. Conclusions: Here we describe 3 LBPs as well as a TAG lipase from the oleaginous microalga L. incisa and discuss their possible involvement in LB metabolism. This study highlights the importance of filtering LB proteome datasets and verifying the subcellular localization one by one, so that contaminating proteins can be recognized as such. Our dataset can serve as a valuable resource in the identification of additional LBPs, shedding more light on the intriguing roles of LBs in microalgae."],["dc.identifier.doi","10.1186/s12870-017-1042-2"],["dc.identifier.isi","000403879900002"],["dc.identifier.pmid","28587627"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14506"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42344"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2229"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]