Napp, Joanna

[["dc.bibliographiccitation.artnumber","089801"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of Biomedical Optics"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Mathejczyk, Julia Eva"],["dc.contributor.author","Pauli, Jutta"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Resch-Genger, Ute"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Napp, Joanna"],["dc.date.accessioned","2018-11-07T09:21:43Z"],["dc.date.available","2018-11-07T09:21:43Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1117/1.JBO.18.8.089801"],["dc.identifier.isi","000324287700025"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29174"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Spie-soc Photo-optical Instrumentation Engineers"],["dc.relation.issn","1083-3668"],["dc.title","High-sensitivity detection of breast tumors in vivo by use of a pH-sensitive near-infrared fluorescence probe (vol 17, 076028, 2012)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","101"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","ChemBioChem"],["dc.bibliographiccitation.lastpage","110"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Pauli, Jutta"],["dc.contributor.author","Pochstein, Marieke"],["dc.contributor.author","Haase, Andrea"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Luch, Andreas"],["dc.contributor.author","Resch-Genger, Ute"],["dc.date.accessioned","2018-11-07T10:28:29Z"],["dc.date.available","2018-11-07T10:28:29Z"],["dc.date.issued","2017"],["dc.description.abstract","The design of bright and functional dye-protein conjugates requires hydrophilic and stable fluorophores with high molar absorption coefficients and high fluorescence quantum yields, which must not be prone to dimerization, as well as conservation of protein function and suppression of protein association. Although many synthetic dyes meet these needs, the influence of dye charge on bioconjugate performance is commonly neglected. This encouraged us to assess the spectroscopic properties, antibody functionality, binding behavior, folding, and association of conjugates of the therapeutic antibodies trastuzumab and cetuximab with the red cyanine dyes S0586, S2381, and 6SIDCC (bearing two, three, and six sulfonate groups, respectively). Our results demonstrate a negligible effect of dye labeling on antibody folding, yet a strong influence of label charge and density on antibody isoelectric points and association. Especially 6SIDCC decreased strongly the isoelectric points of both antibodies and their heavy or light chains even at low labeling degrees, thus favoring protein association. Although an increasingly negative dye charge reduces antigen affinity as shown in a competitive immunoassay, all conjugates still bound to cells overexpressing the target of the respective antibody. Obviously, dyes that cause minimum dimerization with a small number of charged groups are best for conjugate brightness, minimum protein association, and strong target binding. This underlines the need to consider dye charge for the rational design of conjugates with optimum performance."],["dc.identifier.doi","10.1002/cbic.201600299"],["dc.identifier.isi","000392936000011"],["dc.identifier.pmid","27790811"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43432"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.issn","1439-7633"],["dc.relation.issn","1439-4227"],["dc.title","Influence of Label and Charge Density on the Association of the Therapeutic Monoclonal Antibodies Trastuzumab and Cetuximab Conjugated to Anionic Fluorophores"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","469"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Molecular Imaging"],["dc.bibliographiccitation.lastpage","480"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Mathejczyk, Julia Eva"],["dc.contributor.author","Pauli, Jutta"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Tietze, Lutz F."],["dc.contributor.author","Kessler, Horst"],["dc.contributor.author","Resch-Genger, Ute"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2018-11-07T08:50:00Z"],["dc.date.available","2018-11-07T08:50:00Z"],["dc.date.issued","2011"],["dc.description.abstract","Labeling of RGD peptides with near-infrared fluorophores yields optical probes for noninvasive imaging of tumors overexpressing alpha(v)beta(3) integrins. An important prerequisite for optimum detection sensitivity in vivo is strongly absorbing and highly emissive probes with a known fluorescence lifetime. The RGD-Cy5.5 optical probe was derived by coupling Cy5.5 to a cyclic arginine-glycine-aspartic acid-D-phenylalanine lysine (RGDfK) peptide via an aminohexanoic acid spacer. Spectroscopic properties of the probe were studied in different matrices in comparison to Cy5.5. For in vivo imaging, human glioblastoma cells were subcutaneously implanted into nude mice, and in vivo fluorescence intensity and lifetime were measured. The fluorescence quantum yield and lifetime of Cy5.5 were found to be barely affected on RGD conjugation but dramatically changed in the presence of proteins. By time domain fluorescence imaging, we demonstrated specific binding of RGD-Cy5.5 to glioblastoma xenografts in nude mice. Discrimination of unspecific fluorescence by lifetime-gated analysis further enhanced the detection sensitivity of RGD-Cy5.5-derived signals. We characterized RGD-Cy5.5 as a strongly emissive and stable probe adequate for selective targeting of alpha(v)beta(3) integrins. The specificity and thus the overall detection sensitivity in vivo were optimized with lifetime gating, based on the previous determination of the probes fluorescence lifetime under application-relevant conditions."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [Sonderforschungsbereich 416]"],["dc.identifier.doi","10.2310/7290.2011.00018"],["dc.identifier.isi","000299129200007"],["dc.identifier.pmid","22201538"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21589"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.notes.submitter","Najko"],["dc.relation.issn","1535-3508"],["dc.title","Spectroscopically Well-Characterized RGD Optical Probe as a Prerequisite for Lifetime-Gated Tumor Imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9039"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Analytical Chemistry"],["dc.bibliographiccitation.lastpage","9046"],["dc.bibliographiccitation.volume","83"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Behnke, Thomas"],["dc.contributor.author","Fischer, Lorenz"],["dc.contributor.author","Wuerth, Christian"],["dc.contributor.author","Wottawa, Marieke"],["dc.contributor.author","Katschinski, Doerthe Magdalena"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Resch-Genger, Ute"],["dc.contributor.author","Schaeferling, Michael"],["dc.date.accessioned","2018-11-07T08:49:19Z"],["dc.date.available","2018-11-07T08:49:19Z"],["dc.date.issued","2011"],["dc.description.abstract","Polystyrene nanoparticles (PS-NPs) were doped with an oxygen-sensitive near-infrared (NIR)-emissive palladium meso-tetraphenylporphyrin and an inert reference dye which are both excitable at 635 nm. The nanosensors were characterized with special emphasis on fundamental parameters such as absolute photoluminescence quantum yield and fluorescence lifetime. The PS-NPs were employed for ratiometric dual-wavelength and lifetime-based photoluminescent oxygen sensing. They were efficiently taken up by cultured murine alveolar macrophages, yielding a characteristic and reversible change in ratiometric response with decreasing oxygen concentration. This correlated with the cellular hypoxic status verified by analysis of hypoxia inducible factor-1 alpha (HIF-1 alpha) accumulation. In addition, the surface of PS-NPs was functionalized with polyethylene glycol (PEG) and the monoclonal antibody herceptin, and their binding to HER2/neu-overexpressing tumor cells was confirmed in vitro. First experiments with tumor-bearing mouse revealed a distinctive ratiometric response within the tumor upon hypoxic condition induced by animal sacrifice. These results demonstrate the potential of these referenced NIR nanosensors for in vitro and in vivo imaging that present a new generation of optical probes for oncology."],["dc.identifier.doi","10.1021/ac201870b"],["dc.identifier.isi","000297481700032"],["dc.identifier.pmid","22007722"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21431"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1520-6882"],["dc.relation.issn","0003-2700"],["dc.title","Targeted Luminescent Near-Infrared Polymer-Nanoprobes for In Vivo Imaging of Tumor Hypoxia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","11642"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","11657"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Markus, M. Andrea"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Behnke, Thomas"],["dc.contributor.author","Mitkovski, Miso"],["dc.contributor.author","Monecke, Sebastian"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Kilfeather, Stephen"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Resch-Genger, Ute"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2018-11-07T09:48:05Z"],["dc.date.available","2018-11-07T09:48:05Z"],["dc.date.issued","2015"],["dc.description.abstract","Molecular imaging of inflammatory lung diseases, such as asthma, has been limited to date. The recruitment of innate immune cells to the airways is central to the inflammation process. This study exploits these cells for imaging purposes within the lung, using inhaled polystyrene nanoparticles loaded with the near-infrared fluorescence dye Itrybe (Itrybe-NPs). By means of in vivo and ex vivo fluorescence reflectance imaging of an ovalbumin-based allergic airway inflammation (AA!) model in hairless SKH-1 mice, we show that subsequent to intranasal application of Itrybe-NPs, AAI lungs display fluorescence intensities significantly higher than those in lungs of control mice for at least 24 h. Ex vivo immunofluorescence analysis of lung tissue demonstrates the uptake of Itrybe-NPs predominantly by CD68(+)CD11c(+)ECF-(L+MHCIIlow) cells, identifying them as alveolar M2 macrophages in the peribronchial and alveolar areas. The in vivo results were validated by confocal microscopy, overlapping tile analysis, and flow cytometry, showing an amount of Itrybe-NP-containing macrophages in lungs of AAI mice significantly larger than that in controls. A small percentage of NP-containing cells were identified as dendritic cells. Flow cytometry of tracheobronchial lymph nodes showed that Itrybe-NPs were negligible in lung draining lymph nodes 24 h after inhalation. This imaging approach may advance preclinical monitoring of AAI in vivo over time and aid the investigation of the role that macrophages play during lung inflammation. Furthermore, it allows for tracking of inhaled nanoparticles and can hence be utilized for studies of the fate of potential new nanotherapeutics."],["dc.identifier.doi","10.1021/acsnano.5b04026"],["dc.identifier.isi","000367280100014"],["dc.identifier.pmid","26513457"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35240"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/97"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C05: Bedeutung von zellulären Immunreaktionen für das kardiale Remodeling und die Therapie der Herzinsuffizienz durch Stammzelltransplantation"],["dc.relation.issn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.relation.workinggroup","RG Dressel"],["dc.title","Tracking of Inhaled Near-Infrared Fluorescent Nanoparticles in Lungs of SKH-1 Mice with Allergic Airway Inflammation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","076028"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Biomedical Optics"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Mathejczyk, Julia Eva"],["dc.contributor.author","Pauli, Jutta"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Resch-Genger, Ute"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Napp, Joanna"],["dc.date.accessioned","2018-11-07T09:08:23Z"],["dc.date.available","2018-11-07T09:08:23Z"],["dc.date.issued","2012"],["dc.description.abstract","We investigated the potential of the pH-sensitive dye, CypHer5E, conjugated to Herceptin (pH-Her) for the sensitive detection of breast tumors in mice using noninvasive time-domain near-infrared fluorescence imaging and different methods of data analysis. First, the fluorescence properties of pH-Her were analyzed as function of pH and/or dye-to-protein ratio, and binding specificity was confirmed in cell-based assays. Subsequently, the performance of pH-Her in nude mice bearing orthotopic HER2-positive (KPL-4) and HER2-negative (MDA-MB-231) breast carcinoma xenografts was compared to that of an always-on fluorescent conjugate Alexa Fluor 647-Herceptin (Alexa-Her). Subtraction of autofluorescence and lifetime (LT)-gated image analyses were performed for background fluorescence suppression. In mice bearing HER2-positive tumors, autofluorescence subtraction together with the selective fluorescence enhancement of pH-Her solely in the tumor's acidic environment provided high contrast-to-noise ratios (CNRs). This led to an improved sensitivity of tumor detection compared to Alexa-Her. In contrast, LT-gated imaging using LTs determined in model systems did not improve tumor-detection sensitivity in vivo for either probe. In conclusion, pH-Her is suitable for sensitive in vivo monitoring of HER2-expressing breast tumors with imaging in the intensity domain and represents a promising tool for detection of weak fluorescent signals deriving from small tumors or metastases. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.7.076028]"],["dc.description.sponsorship","DFG [AL336/5-2 Alves, SPP1190]"],["dc.identifier.doi","10.1117/1.JBO.17.7.076028"],["dc.identifier.isi","000307989500055"],["dc.identifier.pmid","22894511"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26020"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Spie-soc Photo-optical Instrumentation Engineers"],["dc.relation.issn","1083-3668"],["dc.title","High-sensitivity detection of breast tumors in vivo by use of a pH-sensitive near-infrared fluorescence probe"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","160"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Biomaterials"],["dc.bibliographiccitation.lastpage","170"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Behnke, Thomas"],["dc.contributor.author","Mathejczyk, Julia Eva"],["dc.contributor.author","Brehm, Robert"],["dc.contributor.author","Wuerth, Christian"],["dc.contributor.author","Gomes, Fernanda Ramos"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Resch-Genger, Ute"],["dc.date.accessioned","2018-11-07T09:31:04Z"],["dc.date.available","2018-11-07T09:31:04Z"],["dc.date.issued","2013"],["dc.description.abstract","Current optical probes including engineered nanoparticles (NPs) are constructed from near infrared (NIR)-emissive organic dyes with narrow absorption and emission bands and small Stokes shifts prone to aggregation-induced self-quenching. Here, we present the new asymmetric cyanine Itrybe with broad, almost environment-insensitive absorption and emission bands in the diagnostic window, offering a unique flexibility of the choice of excitation and detection wavelengths compared to common NIR dyes. This strongly emissive dye was spectroscopically studied in different solvents and encapsulated into differently sized (15, 25, 100 nm) amino-modified polystyrene NPs (PSNPs) via a one-step staining procedure. As proof-of-concept for its potential for pre-/clinical imaging applications, Itrybe-loaded NPs were surface-functionalized with polyethylene glycol (PEG) and the tumor-targeting antibody Herceptin and their binding specificity to the tumor-specific biomarker HER2 was systematically assessed. Itrybe-loaded NPs display strong fluorescence signals in vitro and in vivo and Herceptin-conjugated NPs bind specifically to HER2 as demonstrated in immunoassays as well as on tumor cells and sections from mouse tumor xenografts in vitro. This demonstrates that our design strategy exploiting broad band-absorbing and -emitting dyes yields versatile and bright NIR probes with a high potential for e.g. the sensitive detection and characterization of tumor development and progression. (C) 2012 Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.biomaterials.2012.09.028"],["dc.identifier.isi","000311532400016"],["dc.identifier.pmid","23072943"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31456"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.relation.issn","1878-5905"],["dc.relation.issn","0142-9612"],["dc.title","Target-specific nanoparticles containing a broad band emissive NIR dye for the sensitive detection and characterization of tumor development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]