Napp, Joanna

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Public Library of Science"],["dc.bibliographiccitation.lastpage","11"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Rolf, Hans J."],["dc.contributor.author","Kierdorf, Uwe"],["dc.contributor.author","Kierdorf, Horst"],["dc.contributor.author","Schulz, Jutta"],["dc.contributor.author","Seymour, Natascha"],["dc.contributor.author","Schliephake, Henning"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Niebert, Sabine"],["dc.contributor.author","Wölfel, Helmuth"],["dc.contributor.author","Wiese, K. Günter"],["dc.date.accessioned","2019-07-10T08:12:58Z"],["dc.date.available","2019-07-10T08:12:58Z"],["dc.date.issued","2008-04-30"],["dc.description.abstract","The annual regeneration of deer antlers is a unique developmental event in mammals, which as a rule possess only a very limited capacity to regenerate lost appendages. Studying antler regeneration can therefore provide a deeper insight into the mechanisms that prevent limb regeneration in humans and other mammals, and, with regard to medical treatments, may possibly even show ways how to overcome these limitations. Traditionally, antler regeneration has been characterized as a process involving the formation of a blastema from de-differentiated cells. More recently it has, however, been hypothesized that antler regeneration is a stem cell-based process. Thus far, direct evidence for the presence of stem cells in primary or regenerating antlers was lacking. Here we demonstrate the presence of cells positive for the mesenchymal stem cell marker STRO-1 in the chondrogenic growth zone and the perivascular tissue of the cartilaginous zone in primary and regenerating antlers as well as in the pedicle of fallow deer (Dama dama). In addition, cells positive for the stem cell/progenitor cell markers STRO-1, CD133 and CD271 (LNGFR) were isolated from the growth zones of regenerating fallow deer antlers as well as the pedicle periosteum and cultivated for extended periods of time. We found evidence that STRO-1+ cells isolated from the different locations are able to differentiate in vitro along the osteogenic and adipogenic lineages. Our results support the view that the annual process of antler regeneration might depend on the periodic activation of mesenchymal progenitor cells located in the pedicle periosteum. The findings of the present study indicate that not only limited tissue regeneration, but also extensive appendage regeneration in a postnatal mammal can occur as a stem cell-based process."],["dc.identifier.fs","187915"],["dc.identifier.ppn","574912630"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4343"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61087"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","CC BY 2.5"],["dc.rights.uri","http://creativecommons.org/licenses/by/2.5"],["dc.subject.ddc","570"],["dc.subject.ddc","630"],["dc.title","Localization and Characterization of STRO-1+ Cells in the Deer Pedicle and Regenerating Antler"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","379"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Clinical Oral Implants Research"],["dc.bibliographiccitation.lastpage","386"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Materna, Thomas"],["dc.contributor.author","Rolf, Hans J."],["dc.contributor.author","Napp, Johanna"],["dc.contributor.author","Schulz, Jutta"],["dc.contributor.author","Gelinsky, Michael"],["dc.contributor.author","Schliephake, Henning"],["dc.date.accessioned","2018-11-07T11:16:24Z"],["dc.date.available","2018-11-07T11:16:24Z"],["dc.date.issued","2008"],["dc.description.abstract","Aims: The aim of the present report was to evaluate current methods of in vitro analysis of three-dimensional (3D) scaffolds seeded with human bone marrow stromal cells (hBMSCs) from six bone marrow aspirates for tissue engineered growth of bone. Methods: A series of experiments was conducted to compare methods of cell expansion and to validate analysis of proliferation and differentiation of hBMSCs in long term cultures of up to 40 days in 3D scaffolds of calcium carbonate (CaCO3) and mineralized collagen. Proliferation within the seeded scaffolds was monitored using cell counting, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), neutral red (NR) and DNA fluorescence assays and compared with empty controls. Differentiation was assessed by means of ELISA for osteocalcin (OC) and real time PCR for OC and collagen I (Coll I). Results: The results showed that the scaffold differed in seeding efficacy (CaCO3: 53.3%, min. Coll.: 83.3%). The precise identification of the number of cells in biomaterials by MTT, NR and DNA assays was problematic, as MTT and NR assay overestimated the number of cells, whereas DNA assay grossly underestimated the number of cells on the scaffolds. Monitoring of changes over time may be biased by unspecific material-dependent background activity that has to be taken into account. Identification of osteogenic differentiation is not reliable by identifying osteogenic markers such as OC in the supernatant but has to be done on the transcriptional level. Conclusions: It is concluded that monitoring of in vitro procedures for the construction of biohybrid scaffolds requires more emphasis in order to make the cell based approach a reliable treatment option in tissue engineering."],["dc.identifier.doi","10.1111/j.1600-0501.2007.01483.x"],["dc.identifier.isi","000253757900009"],["dc.identifier.pmid","18324959"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54577"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0905-7161"],["dc.title","In vitro characterization of three-dimensional scaffolds seeded with human bone marrow stromal cells for tissue engineered growth of bone: mission impossible? A methodological approach"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]