Cheng, I-Fen

[["dc.bibliographiccitation.firstpage","65"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cellular Reprogramming"],["dc.bibliographiccitation.lastpage","76"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Streckfuss-Bömeke, Katrin"],["dc.contributor.author","Jende, Jörg"],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2017-09-07T11:52:34Z"],["dc.date.available","2017-09-07T11:52:34Z"],["dc.date.issued","2014"],["dc.description.abstract","On the basis of their self-renewal capacity and their ability to differentiate into derivatives of all three germ layers, germ line–derived multipotent adult stem cells (maGSCs) from mouse testis might serve as one of preferable sources for pluripotent stem cells in regenerative medicine. In our study, we aimed for an efficient hepatic differentiation protocol that is applicable for both maGSCs and embryonic stem cells (ESCs). We attempted to accomplish this goal by using a new established co-culture system with OP9 stroma cells for direct differentiation of maGSCs and ESCs into hepatic cells. We found that the hepatic differentiation of maGSCs was induced by the OP9 co-culture system in comparison to the gelatin culture. Furthermore, we showed that the combination of OP9 co-culture with activin A resulted in the increased expression of endodermal and early hepatic markers Gata4, Sox17, Foxa2, Hnf4, Afp, and Ttr compared to differentiated cells on gelatin or on OP9 alone. Moreover, the hepatic progenitors were capable of differentiating further into mature hepatic cells, demonstrated by the expression of liver-specific markers Aat, Alb, Tdo2, Krt18, Krt8, Krt19, Cps1, Sek, Cyp7a1, Otc, and Pah. A high percentage of maGSC-derived hepatic progenitors (51% AFP- and 61% DLK1-positive) and mature hepatic-like cells (26% ALB-positive) were achieved using this OP9 co-culture system. These generated hepatic cells successfully demonstrated in vitro functions associated with mature hepatocytes, including albumin and urea secretion, glycogen storage, and uptake of low-density lipoprotein. The established co-culture system for maGSCs into functional hepatic cells might serve as a suitable model to delineate the differentiation process for the generation of high numbers of mature hepatocytes in humans without genetic manipulations and make germ line–derived stem cells a potential autologous and alternative cell source for hepatic transplants in metabolic liver disorders."],["dc.identifier.doi","10.1089/cell.2013.0057"],["dc.identifier.gro","3144969"],["dc.identifier.pmid","24380658"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2652"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","2152-4971"],["dc.title","Efficient Generation of Hepatic Cells from Multipotent Adult Mouse Germ-Line Stem Cells Using an OP9 Co-Culture System"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Heida, Nana-Maria"],["dc.contributor.author","Schroeter, M. R."],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Mueller, Jan-Peter"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Konstantindes, Stavros"],["dc.contributor.author","Schaefer, Katrin"],["dc.date.accessioned","2018-11-07T10:57:45Z"],["dc.date.available","2018-11-07T10:57:45Z"],["dc.date.issued","2007"],["dc.format.extent","839"],["dc.identifier.isi","000250394303788"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50323"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","80th Annual Scientific Session of the American-Heart-Association"],["dc.relation.eventlocation","Orlando, FL"],["dc.relation.issn","0009-7322"],["dc.title","Effects of obesity and weight loss on the functional properties of endothelial progenitor cells"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","200"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Arteriosclerosis, Thrombosis, and Vascular Biology"],["dc.bibliographiccitation.lastpage","206"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Heida, Nana-Maria"],["dc.contributor.author","Leifheit-Nestler, Maren"],["dc.contributor.author","Schroeter, Marco R."],["dc.contributor.author","Mueller, Jan-Peter"],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Henkel, Sarah"],["dc.contributor.author","Limbourg, Anne"],["dc.contributor.author","Limbourg, Florian P."],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Quigley, James P."],["dc.contributor.author","Ruggeri, Zaverio M."],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Konstantinides, Stavros"],["dc.contributor.author","Schaefer, Katrin"],["dc.date.accessioned","2017-09-07T11:46:10Z"],["dc.date.available","2017-09-07T11:46:10Z"],["dc.date.issued","2010"],["dc.description.abstract","Objective-To investigate the capacity of the adipokine leptin to promote angiogenesis by modulating the function of circulating angiogenic cells (CACs). Methods and Results-In vitro, leptin specifically promoted CAC adhesion to tubular endothelial structures and migration along outgrowing sprouts of endothelial cells. In vivo, stimulation of CACs with leptin increased their capacity to promote new vessel formation in the chorioallantoic membrane of chicken embryos and to improve neovascularization of ischemic murine hind limbs. These effects required the phosphorylation of alpha v beta 5 integrins, which depended on the interaction of leptin with its receptor ObR, and on Janus kinase (JAK) 2- and phospholipase C (PLC) gamma-mediated activation of Src kinase. Protein tyrosine phosphatase 1B, a negative regulator of leptin signaling, was overexpressed in CACs from obese, hyperleptinemic individuals, and this was associated with insensitivity of CACs to the angiogenic effects of leptin. Weight loss (by 30 +/- 15 kg) normalized protein tyrosine phosphatase 1B expression in CACs and restored their responsiveness to leptin. A similar dose- dependent response was found after incubation of CACs from obese subjects with a protein tyrosine phosphatase 1B inhibitor ex vivo. Conclusion-Our results point to the ObR-Src kinase-alpha v beta 5 cross talk as a distinct novel component of the network of specific interactions between integrins and cytokine receptors in angiogenesis. (Arterioscler Thromb Vasc Biol. 2010; 30: 200-206.)"],["dc.identifier.doi","10.1161/ATVBAHA.109.192807"],["dc.identifier.gro","3142969"],["dc.identifier.isi","000273799900014"],["dc.identifier.pmid","19910644"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/431"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: National Institutes of Health [NIH HL-75736]"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1079-5642"],["dc.title","Leptin Enhances the Potency of Circulating Angiogenic Cells Via Src Kinase and Integrin alpha v beta 5 Implications for Angiogenesis in Human Obesity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Heida, Nana-Maria"],["dc.contributor.author","Schroeter, M. R."],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Deryugina, Elena I."],["dc.contributor.author","Korff, Thomas"],["dc.contributor.author","Quigley, James P."],["dc.contributor.author","Ruggeri, Zaverio M."],["dc.contributor.author","Mueller, Jan-Peter"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Konstantmides, Stavros"],["dc.contributor.author","Schaefer, Katrin"],["dc.date.accessioned","2018-11-07T10:57:41Z"],["dc.date.available","2018-11-07T10:57:41Z"],["dc.date.issued","2007"],["dc.format.extent","175"],["dc.identifier.isi","000250394300776"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50313"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","80th Annual Scientific Session of the American-Heart-Association"],["dc.relation.eventlocation","Orlando, FL"],["dc.relation.issn","0009-7322"],["dc.title","Leptin potentiates the angiogenic properties of endothelial progenitor cells in vitro and in vivo"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","122"],["dc.contributor.author","Schroeter, M. R."],["dc.contributor.author","Stein, Susanne"],["dc.contributor.author","Heida, Nana-Maria"],["dc.contributor.author","Leifheit-Nestler, Maren"],["dc.contributor.author","Christiansen, Hans"],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Shah, Ajay M."],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Konstantinides, Stavros V."],["dc.contributor.author","Schaefer, Katrin"],["dc.date.accessioned","2018-11-07T08:36:40Z"],["dc.date.available","2018-11-07T08:36:40Z"],["dc.date.issued","2010"],["dc.identifier.isi","000208231600521"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18371"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.issn","0009-7322"],["dc.title","Leptin Promotes the Mobilization of Fetal Liver Kinase 1-Positive Vascular Progenitor Cells From the Bone Marrow in a NOX2/MMP9-dependent Manner and Enhances Neovascularization after Ischemia"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Heida, Nana-Maria"],["dc.contributor.author","Mueller, Jan-Peter"],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Konstantinides, Stavros V."],["dc.contributor.author","Schaefer, Katrin"],["dc.date.accessioned","2018-11-07T11:09:53Z"],["dc.date.available","2018-11-07T11:09:53Z"],["dc.date.issued","2008"],["dc.format.extent","S509"],["dc.identifier.isi","000262104501258"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53098"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","81st Annual Scientific Session of the American-Heart-Association"],["dc.relation.eventlocation","New Orleans, LA"],["dc.relation.issn","0009-7322"],["dc.title","Mechanisms Mediating The Proangiogenic Effects Of Leptin On Endothelial Progenitor Cells From Lean And Obese Individuals"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","170"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cardiovascular Research"],["dc.bibliographiccitation.lastpage","180"],["dc.bibliographiccitation.volume","93"],["dc.contributor.author","Schroeter, Marco R."],["dc.contributor.author","Stein, Susanne"],["dc.contributor.author","Heida, Nana-Maria"],["dc.contributor.author","Leifheit-Nestler, Maren"],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Gogiraju, Rajinikanth"],["dc.contributor.author","Christiansen, Hans"],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Shah, Ajay M."],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Konstantinides, Stavros"],["dc.contributor.author","Schaefer, Katrin"],["dc.date.accessioned","2017-09-07T11:43:14Z"],["dc.date.available","2017-09-07T11:43:14Z"],["dc.date.issued","2012"],["dc.description.abstract","Aims Bone marrow (BM) progenitors participate in new vessel formation and endothelial repair. The leptin receptor (ObR) is expressed on hematopoietic cells; however, the effects of leptin on BM progenitor cells and their angiogenic potential are unknown. Methods and results In the present study, we show that the short-term administration of leptin (over five consecutive days) into wild-type mice increased the number of circulating, BM-derived sca-1(+), flk-1(+) vascular progenitors, 95 +/- 1.7% of which also expressed ObR. Ex vivo stimulation of BM cells with leptin enhanced the expression of NADPH oxidase isoform 2 (NOX2), and the leptin-induced increase in reactive oxygen species production, matrix metalloproteinase-9 (MMP9) expression and circulating soluble KitL levels was absent in mice lacking NOX2. Furthermore, intraperitoneal injections of leptin improved perfusion and increased the number of BM-derived, CD31-positive endothelial cells in ischaemic hindlimbs after femoral artery ligation. The effects of leptin on the mobilization of sca-1(+), flk-1(+) cells and neovascularization were abolished in mice transplanted with BM from ObR-deficient and in NOX2(-/-) mice. Conclusion Our findings suggest that the angiogenic effects of leptin involve sca-1(+), flk-1(+) vascular progenitor cells mobilized from the BM in response to ObR-mediated activation of NOX2, increased MMP9 expression, and sKitL release."],["dc.identifier.doi","10.1093/cvr/cvr275"],["dc.identifier.gro","3142608"],["dc.identifier.isi","000298381600022"],["dc.identifier.pmid","22065732"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0008-6363"],["dc.title","Leptin promotes the mobilization of vascular progenitor cells and neovascularization by NOX2-mediated activation of MMP9"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","39"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Future Cardiology"],["dc.bibliographiccitation.lastpage","51"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Baazm, Maryam"],["dc.date.accessioned","2021-06-01T10:48:38Z"],["dc.date.available","2021-06-01T10:48:38Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.2217/fca.11.78"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86002"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1744-8298"],["dc.relation.issn","1479-6678"],["dc.title","Human spermatagonial stem cells: a novel therapeutic hope for cardiac regeneration and repair?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Molecular and Cellular Cardiology"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Kaiser, Diana"],["dc.contributor.author","Cheng, I.-F."],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Schaefer, K."],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2018-11-07T11:10:39Z"],["dc.date.available","2018-11-07T11:10:39Z"],["dc.date.issued","2008"],["dc.format.extent","S1"],["dc.identifier.doi","10.1016/j.yjmcc.2008.09.592"],["dc.identifier.isi","000260844600003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53253"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Ltd- Elsevier Science Ltd"],["dc.publisher.place","London"],["dc.relation.conference","25th Annual Meeting of the Japanese Section of the International-Society-for-Heart-Research"],["dc.relation.eventlocation","Yokohama, JAPAN"],["dc.relation.issn","1095-8584"],["dc.relation.issn","0022-2828"],["dc.title","Distingished Award Lecture Adult Pluripotent Cells for Cardiac Regeneration"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Human Gene Therapy"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Kaiser, Diana"],["dc.contributor.author","Cheng, I-Fen"],["dc.contributor.author","Unsoeld, Bernhard W."],["dc.contributor.author","Toischer, Karl"],["dc.contributor.author","Schaefer, Katrin"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2018-11-07T11:22:42Z"],["dc.date.available","2018-11-07T11:22:42Z"],["dc.date.issued","2009"],["dc.format.extent","1387"],["dc.identifier.isi","000271441000119"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56032"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mary Ann Liebert Inc"],["dc.publisher.place","New rochelle"],["dc.relation.conference","Combined Meeting of the 17th European-Society-of-Gene-and-Cell-Therapy/16th German-Society-for-Gene-Therapy/4th German-Society-for-Stem-Cell-Research"],["dc.relation.eventlocation","Hannover, GERMANY"],["dc.relation.issn","1043-0342"],["dc.title","Generation of cardiovascular progenitors from multipotent adult mouse germline stem cells"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]