Niedmann, Paul Dieter

[["dc.bibliographiccitation.firstpage","107"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Clinical Biochemistry"],["dc.bibliographiccitation.lastpage","113"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Wieland, Eberhard"],["dc.contributor.author","Shipkova, Maria"],["dc.contributor.author","Schellhaas, Ulrike"],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Niedmann, Paul Dieter"],["dc.contributor.author","Armstrong, Victor William"],["dc.contributor.author","Oellerich, Michael"],["dc.date.accessioned","2021-06-01T10:50:05Z"],["dc.date.available","2021-06-01T10:50:05Z"],["dc.date.issued","2000"],["dc.description.abstract","Objectives: We have identified an acyl glucuronide (M-2) of the immunosuppressant mycophenolic acid (MPA). Acyl glucuronides have toxic potential and may contribute to drug toxicity. Whether acyl glucuronides are able to induce release of proinflammatory cytokines is unknown. Gastrointestinal disturbances have been observed during MPA therapy and may involve an inflammatory reaction. This study investigated whether M-2 can induce IL-6 and TNF-alpha release as well as gene expression of these cytokines in leukocytes. Design and methods: M-2 was produced by incubation of MPA with human liver microsomes. Human mononuclear leukocytes were incubated in the presence of M-2. Concentrations of IL-6 and TNF-alpha were measured by ELISA. Expression of mRNA was determined by quantitative RT-PCR. Results: Incubation of 3 x 10(6) cells with M-2 resulted in a time and dose dependent release of cytokines, whereas MPA or its phenolic glucuronide MPAG were without effect. Cytokine liberation depended on mRNA induction. Response to M-2 showed much inter individual variability (30-fold for IL-6, 3-fold for TNF-alpha). Conclusions: If M-2 promotes release of cytokines in vivo, these may mediate some of the toxic actions of MPA. Copyright (C) 2000 The Canadian Society of Clinical Chemists."],["dc.identifier.doi","10.1016/S0009-9120(99)00101-0"],["dc.identifier.isi","000086461900005"],["dc.identifier.pmid","10751588"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86520"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.relation.issn","0009-9120"],["dc.title","Induction of cytokine release by the acyl glucuronide of mycophenolic acid: A link to side effects?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","53"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Clinical Biochemistry"],["dc.bibliographiccitation.lastpage","57"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Wieland, Eberhard"],["dc.contributor.author","Shipkova, Maria"],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Braun, Felix"],["dc.contributor.author","Niedmann, Paul-Dieter"],["dc.contributor.author","Armstrong, Victor W."],["dc.contributor.author","Ringe, Burckhardt"],["dc.contributor.author","Svinarov, Dobrin A."],["dc.contributor.author","Oellerich, Michael"],["dc.date.accessioned","2021-06-01T10:50:04Z"],["dc.date.available","2021-06-01T10:50:04Z"],["dc.date.issued","2001"],["dc.description.abstract","Objectives: Little is known about the effect of ischemia/reperfusion with xenogenic blood on function and gene expression of CYP3A4, the enzyme largely responsible for the metabolism of the immunosuppressants Cyclosporin A (CsA) and Tacrolimus. Design and methods: In a pig liver perfusion model, we have compared the effect of perfusion (3 h) after 20 h cold storage, with either pig or human blood on CsA metabolism and CYP3A4-mRNA expression. CYP3A4-mRNA was quantified by RT-PCR, CsA and its major metabolites AM1, AM9, AM4N by RP-HPLC. IL-6 served as inflammation marker, GLDH and ALT to estimate tissue damage. Results: Inflammatory response and tissue damage were more extensive during xenoperfusion. CYP3A4 expression decreased similarly during xenogenic and allogenic perfusion. CsA conversion to its metabolites was also comparable during xeno- and alloperfusion. Conclusion: There is no evidence that during the early reperfusion period pig liver CYP3A4 is severely affected if the organ is xenoperfused with human blood in comparison with alloperfusion. (C) 2001 The Canadian Society of Clinical Chemists. All rights reserved."],["dc.identifier.doi","10.1016/S0009-9120(00)00203-4"],["dc.identifier.isi","000169234600010"],["dc.identifier.pmid","11239516"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86518"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.publisher.place","Oxford"],["dc.relation.conference","Analytical Conference 2000"],["dc.relation.eventlocation","MUNICH, GERMANY"],["dc.relation.issn","0009-9120"],["dc.title","Preliminary report on the effect of xenoperfusion with human blood on cyclosporin A metabolism and cytochrome-P-4503A4-mRNA expression in a pig liver perfusion model"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]