Sibold, Jeremias

[["dc.bibliographiccitation.firstpage","15630"],["dc.bibliographiccitation.issue","28"],["dc.bibliographiccitation.journal","Physical Chemistry, Chemical Physics"],["dc.bibliographiccitation.lastpage","15638"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Bosse, Mathias"],["dc.contributor.author","Sibold, Jeremias"],["dc.contributor.author","Scheidt, Holger A."],["dc.contributor.author","Patalag, Lukas J."],["dc.contributor.author","Kettelhoit, Katharina"],["dc.contributor.author","Ries, Annika"],["dc.contributor.author","Werz, Daniel B."],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Huster, Daniel"],["dc.date.accessioned","2020-12-10T18:11:27Z"],["dc.date.available","2020-12-10T18:11:27Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1039/C9CP02501D"],["dc.identifier.eissn","1463-9084"],["dc.identifier.issn","1463-9076"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16738"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74015"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY-NC 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/3.0"],["dc.title","Shiga toxin binding alters lipid packing and the domain structure of Gb 3 -containing membranes: a solid-state NMR study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2171"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Chembiochem : a European journal of chemical biology"],["dc.bibliographiccitation.lastpage","2178"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Patalag, Lukas J."],["dc.contributor.author","Sibold, Jeremias"],["dc.contributor.author","Schütte, Ole M."],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Werz, Daniel B."],["dc.date.accessioned","2018-01-17T13:01:43Z"],["dc.date.available","2018-01-17T13:01:43Z"],["dc.date.issued","2017"],["dc.description.abstract","Glycosphingolipids are involved in a number of physiological and pathophysiological processes, and they serve as receptors for a variety of bacterial toxins and viruses. To investigate their function in lipid membranes, fluorescently labeled glycosphingolipids are highly desirable. Herein, a synthetic route to access Gb3 glycosphingolipids with fluorescently labeled fatty acids, consisting of pentaene and hexaene moieties either at the terminus or in the middle of the acyl chain, has been developed. The fluorescent properties of the Gb3 derivatives were investigated in small unilamellar vesicles composed of a raft-like mixture. Phase-separated giant unilamellar vesicles (GUVs) allowed the quantification of the apparent partitioning coefficients of the Gb3 compounds by means of confocal fluorescence laser scanning microscopy. The determined partition coefficients demonstrate that the Gb3 derivatives are preferentially localized in the liquid-disordered (ld ) phase. To analyze whether the compounds behave like their physiological counterparts, Cy3-labeled (Cy: cyanine) Shiga toxin B subunits (STxB) were specifically bound to Gb3 -doped GUVs. However, the protein was favorably localized in the ld phase, in contrast to results reported for STxB bound to naturally occurring Gb3 , which is discussed in terms of the packing density of the lipids in the liquid-ordered (lo ) phase."],["dc.identifier.doi","10.1002/cbic.201700414"],["dc.identifier.pmid","28941080"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11699"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1439-7633"],["dc.title","Gb3 Glycosphingolipids with Fluorescent Oligoene Fatty Acids: Synthesis and Phase Behavior in Model Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","17805"],["dc.bibliographiccitation.issue","49"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","17813"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Sibold, Jeremias"],["dc.contributor.author","Kettelhoit, Katharina"],["dc.contributor.author","Vuong, Loan"],["dc.contributor.author","Liu, Fangyuan"],["dc.contributor.author","Werz, Daniel B."],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2019-11-13T15:23:59Z"],["dc.date.accessioned","2021-10-27T13:12:45Z"],["dc.date.available","2019-11-13T15:23:59Z"],["dc.date.available","2021-10-27T13:12:45Z"],["dc.date.issued","2019"],["dc.description.abstract","The receptor lipid Gb3 is responsible for the specific internalization of Shiga toxin (STx) into cells. The head group of Gb3 defines the specificity of STx binding, and the backbone with different fatty acids is expected to influence its localization within membranes impacting membrane organization and protein internalization. To investigate this influence, a set of Gb3 glycosphingolipids labeled with a BODIPY fluorophore attached to the head group was synthesized. C24 fatty acids, saturated, unsaturated, α-hydroxylated derivatives, and a combination thereof, were attached to the sphingosine backbone. The synthetic Gb3 glycosphingolipids were reconstituted into coexisting liquid-ordered (lo )/liquid-disordered (ld ) giant unilamellar vesicles (GUVs), and STx binding was verified by fluorescence microscopy. Gb3 with the C24:0 fatty acid partitioned mostly in the lo phase, while the unsaturated C24:1 fatty acid distributes more into the ld phase. The α-hydroxylation does not influence its partitioning."],["dc.identifier.doi","10.1002/anie.201910148"],["dc.identifier.eissn","1521-3773"],["dc.identifier.issn","1433-7851"],["dc.identifier.pmid","31529754"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16659"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91719"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.issn","1433-7851"],["dc.relation.orgunit","Fakultät für Chemie"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject","carbohydrates; fatty acids; fluorescence; membranes; toxins"],["dc.subject.ddc","540"],["dc.title","Synthesis of Gb3 Glycosphingolipids with Labeled Head Groups: Distribution in Phase-Separated Giant Unilamellar Vesicles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.contributor.author","Sibold, Jeremias"],["dc.contributor.author","Ahadi, Somayeh"],["dc.contributor.author","Werz, Daniel B."],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2021-04-14T08:23:37Z"],["dc.date.available","2021-04-14T08:23:37Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/s00249-020-01461-w"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80987"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Chemically synthesized Gb3 glycosphingolipids: tools to access their function in lipid membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]